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Results for “Transillumination”

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PubMed1988

Submicrogram quantities of unstained proteins are visualized on polyvinylidene difluoride membranes by transillumination.

Unstained proteins on polyvinylidene difluoride membranes which have been dried and saturated with 20% methanol appear as clear areas when placed on a white light box. Sensitivity is comparable to that obtained with Coomassie Blue R250. This procedure greatly reduces the potential for unintended chemical modification during staining procedures, provides a nondestructive means of determining the major features of a protein pattern on a blot and makes it possible to initiate sequencing within minutes after blotting is complete.

Membranes, Artificial↗
PubMed1989

[Differentiation of organ dose distribution during exposure to plain film and transillumination in relation to the path of the rays].

The analysis of present radiation exposure from exposure parameters with a computer program gives an orientation for the radiation burden to various organs of risk in dependence from geometric imaging parameters also for spot film radiographs taken under fluoroscopy. Recommendations for practical proceeding are derived. Considering the relation between fluoroscopy and spot film dose, minimum field size and optimum field location is important in fluoroscopy. With rare-earth-screens instead of conventional high speed screens a reduction of total dose by 27% can be achieved in conventional myelography, that was used as a model for our calculations.

Adult↗