Research note on the preparation of tomato peptone agar medium.
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A new medium is described for the isolation of P. aeruginosa organisms which have been damaged by chlorination. This medium utilises the fact that with arginine. P. aeruginosa produces a strongly alkaline reaction resulting in an easily identified colour change from gray-green to blue-violet. The indicators used for this purpose are bromothymol blue and cresol red. The medium may be used with either the membrane filter or the liquid enrichment technique. Incubation is at 37 degrees C for 24 or 48 h.
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Basal and meal-stimulated gastric acid secretion was measured before and after a 3-week-period of regular consumption of a calcium carbonate-containing antacid with 6 therapeutic doses per day in normal healthy volunteers. Furthermore the effect of a single large dose of 2000 mg calcium ions was studied on meal-stimulated gastric acid secretion before and after this period. The results suggest that (1) even a large single dose of calcium ions seems to have no major effect on meal-stimulated gastric acid secretion and (2) chronic consumption of calcium carbonate containing antacids over a 3-week-period does not change basal- and meal-stimulated gastric acid secretion in healthy volunteers nor does it increase the response to 2 g calcium ions.
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