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PubMed1979

Further observations on noise levels in infant incubators.

The purpose of this study was to conduct an acoustic analysis of incubator noise under two conditions: when the incubator was associated with different types of life-support equipment; and when impulse noise was created by striking the side of the incubator or by opening and closing the doors of the storage unit. It was found that the life-support equipment increased the overall noise levels of incubators by as much as 15 to 20 dB. Much of this increased energy was in the high frequency region. Impulse signals created by striking the side of the incubator ranged from 130 to 140 dB. A representative impulse for opening the incubator was 92.8 dB, whereas closing the door produced a peak amplitude of 114 dB.

Hearing Loss, Noise-Induced↗
PubMed1977

The reversible antithrombin activity of incubated plasma.

The thrombin time of normal citrated plasma is progressively prolonged on incubation in open glass tubes at 37 degrees C. The phenomenon is dependent on the temperature and duration of incubation, on the pH, and on the concentration of calcium ions present. Platelet-rich citrated plasma fails to exhibit augmented antithrombin activity when similarly incubated, and the addition of washed platelets to platelet-poor plasma inhibits this activity. The clot retarding action of incubated plasma against thrombin is also manifested against Arvin (Ancrod), but not against Reptilase-R. This thrombin time lengthening may be inhibited by incubation with anti-antithrombin III antiserum thus indicating that the phenomenon of thrombin time lengthening is consistent with enhanced activity of antithrombin III. It is unlikely that alterations in the activity of alpha2-macroglobulin, is important in the reduced thrombin-coagulability of incubated plasma. Interference with the polymerisation of fibrin monomers by the physico-chemical changes may contribute to the observed phenomenon.

Ancrod↗
PubMed1999

Is the AIDS incubation time changing? A back-calculation approach.

Contradictory literature was recently published on possible changes in AIDS incubation time over the period 1978-1994. The purpose of this work was to test if a change in incubation time (shortening or lengthening) was observed in France, either globally or in specific transmission groups (homosexual-bisexual men, heterosexual subjects), using a back-calculation approach. An age dependent TSI model (time since infection), which took into account a temporary treatment effect and allowed us to test for a change in the incubation time, was applied to the French AIDS cases (Réseau National de Santé Publique). The EM algorithm was used to maximize the likelihood and the best model was selected on the basis of the likelihood ratio statistic. The analysis on all AIDS cases indicated a shortening of the AIDS incubation time estimated to begin in 1983 (95 per cent CI 1982-1984). This shortening of incubation time was also apparent when analysis was restricted to homosexual-bisexual men and to heterosexual subjects. This shortening corresponded to a median incubation time of 9.6 years (95 per cent CI 8.1-10.5) for people infected at 30 years of age in 1983, versus 12.7 years for people infected at 30 years of age before the change.

Acquired Immunodeficiency Syndrome↗
PubMed2000

Uncertainty of AIDS incubation time and its effects on back-calculation estimates.

Incubation time is the period from the onset of HIV infection to AIDS. The distribution of the incubation time is one of the main parameters of the back-calculation method for the estimation of incidence of HIV infection. Because of the long and variable incubation time, the assessment of its distribution is uncertain and this uncertainty spreads through the back-calculation method and affects the estimation of the precision of incidence of HIV infection. We propose a method to investigate the sensitivity of the estimates to variations of the incubation times, with particular regard to the covariate AGE in the modelling of the incubation period, making use of the parametric bootstrap. An application to the HIV epidemic in Italy is presented. The amplification of the uncertainty of the HIV incidence estimates resulting from the implementation of our proposed method tends to concentrate around the earlier periods of the epidemic, corresponding to the right tail of the incubation time distribution, which is very sensitive to small perturbations.

Acquired Immunodeficiency Syndrome↗
PubMed2006

Effect of inhibition treatment, type of inocula, and incubation temperature on batch H2 production from organic solid waste.

Two types of induction treatments (heat-shock pretreatment, HSP, and acetylene, Ac), inocula (meso and thermophilic) and incubation temperatures (37 and 55 degrees C) were tested according to a full factorial design 2(3) with the aim of assessing their effects on cumulative H(2) production (P(H), mmol H(2)/mini-reactor), initial H(2) production rate (R(i,H), micromol H(2)/(g VS(i) x h)), lag time (T(lag), h), and metabolites distribution when fermenting organic solid waste with an undefined anerobic consortia in batch mini-reactors. Type of inocula did not have a significant effect on P(H), T(lag), and R(i,H) except for organic acids production: mini-reactors seeded with thermophilic inocula had the highest organic acid production. Concerning the induction treatment, it was found that on the average Ac only affected in a positive way the P(H) and T(lag). Thus, P(H) in Ac-inhibited units (6.97) was 20% larger than those in HSP-inhibited units (5.77). Also, Ac favored a shorter T(lag) for P(H) in comparison with HSP (180 vs. 366). Additionally, a positive correlation was found between H(2) and organic acid production. In contrast, solvent concentration in heat-shocked mini-reactors were slightly higher than in reactors spiked with Ac. Regarding the incubation temperature, on the average mesophilic temperature affected in a positive and very significant way P(H) (10.07 vs. 2.67) and R(i,H) (2.43 vs. 0.76) with minimum T(lag) (87 vs. 459). The positive correlation between H(2) and organic acids production was found again. Yet, incubation temperature did not seem to affect solvent production. A strong interaction was observed between induction treatment and incubation temperature. Thus, Ac-inhibited units showed higher values of P(H) and R(i,H) than that HSP-inhibited units only under thermophilic incubation. Contrary to this, HSP-inhibited units showed the highest values of P(H) and R(i,H) only under mesophilic conditions. Therefore, the superiority of an induction treatment seems to strongly depend on the incubation temperature.

Acetylene↗
PubMed1982

Acceleration of the biosynthesis of rat striatal dopamine by incubation and by administration of gamma-butyrolactone.

The concentration of dopamine in striatal slices obtained from rats treated with an MAO inhibitor was increased significantly by a 50-minute incubation in oxygenated Krebs buffer at 37 degrees C. Administration of alpha-methyl-p-tyrosine did not antagonize this small increase in dopamine. The extent of the incubation-induced increase was much greater in tissue obtained from control rats and was also greater than that resulting from the intraperitoneal administration of gamma-butyrolactone (GBL). Prior administration of alpha-methyl-p-tyrosine, d-amphetamine, apomorphine, or lergotrile significantly antagonized the increases in striatal dopamine levels induced by GBL administration or by incubation of striatal slices obtained from control rats. The prior administration of phenylethylamine, however, antagonized the incubation-induced, but not the GBL-induced, increase in striatal dopamine. In conclusion, the effect of incubating striatal slices on their dopamine levels was similar to that of administering GBL in that the incubation enhanced the activity of tyrosine hydroxylase that in turn produced an accelerated synthesis of dopamine.

4-Butyrolactone↗
PubMed1984

Anion permeability of toad skeletal muscle incubated in plasma and ringer solution.

Isolated semitendinosus muscles of the toad were incubated in both Ringer solution and toad plasma for periods ranging from 45 to 170 minutes. The extracellular volume (ECV), as measured from the equilibrium distribution of 35SO4(2-), was found to increase (in gram extracellular (EC) water/gram wet weight) from 0.15 +/- 0.01 to 0.20 +/- 0.02 in muscles incubated in Ringer solution, whereas the ECV was found to remain constant in tissues incubated in plasma (0.16 +/- 0.01 to 0.17 +/- 0.01), for periods up to 170 minutes. Since the true ECV, as measured morphometrically, was found to remain unchanged for muscles incubated in Ringer solution for up to 3 hours, the data for these muscles were interpreted to represent a change in anion permeability of the cellular membranes in response to the absence of some plasma fraction from the Ringer solution (with an electrolyte composition and pH similar to that of plasma). When toad plasma albumin was added to the Ringer solution, in physiological concentrations, the ECV of the muscles was observed to remain relatively constant during the 3-hour incubation period. This albumin effect on anion permeability was found to be albumin concentration-dependent. Further, other sources of albumin were found to have varying degrees of effect on the anion permeability of toad muscle, with bovine albumin serving as the most acceptable substitute for toad albumin, when incubating semitendinosus muscles.

Animals↗
PubMed2005

Modelling the incubation period of anthrax.

Models of the incubation period of anthrax are important to public health planners because they can be used to predict the delay before outbreaks are detected, the size of an outbreak and the duration of time that persons should remain on antibiotics to prevent disease. The difficulty is that there is little direct data about the incubation period in humans. The objective of this paper is to develop and apply models for the incubation period of anthrax. Mechanistic models that account for the biology of spore clearance and germination are developed based on a competing risks formulation. The models predict that the incubation period distribution depends critically on the rate that spores are cleared from the lung and to a lesser extent on the dose of inhaled spores. The models are used in a statistical analysis of data from an anthrax outbreak that occurred in Sverdlovsk, Russia. The analysis suggests that spores are cleared from the lung at a rate between 8 per cent per day and 14 per cent per day, which is in good agreement with experimental studies of animals. The analysis suggests that at low doses, the overall median incubation period time is about 10 days, which includes a median lag of about 2 days between spore germination and onset of symptoms. Male gender and younger ages were associated with longer incubation periods as was lower dose of inhaled spores.

Adult↗
PubMed2005

The estimation of SARS incubation distribution from serial interval data using a convolution likelihood.

The incubation period of SARS is the time between infection of disease and onset of symptoms. Knowledge about the distribution of incubation times is crucial in determining the length of quarantine period and is an important parameter in modelling the spread and control of SARS. As the exact time of infection is unknown for most patients, the incubation time cannot be determined. What is observable is the serial interval which is the time from the onset of symptoms in an index case to the onset of symptoms in a subsequent case infected by the index case. By constructing a convolution likelihood based on the serial interval data, we are able to estimate the incubation distribution which is assumed to be Weibull, and justifications are given to support this choice over other distributions. The method is applied to data provided by the Ministry of Health of Singapore and the results justify the choice of a ten-day quarantine period. The indirect estimate obtained using the method of convolution likelihood is validated by means of comparison with a direct estimate obtained directly from a subset of patients for whom the incubation time can be ascertained. Despite its name, the proposed indirect estimate is actually more precise than the direct estimate because serial interval data are recorded for almost all patients, whereas exact incubation times can be determined for only a small subset. It is possible to obtain an even more efficient estimate by using the combined data but the improvement is not substantial.

Adult↗
PubMed1999

Daily estradiol and progesterone levels relative to laying and onset of incubation in canaries.

Female birds may optimize reproduction by modifying clutch size and the timing for the onset of incubation. We measured fecal estradiol-17beta (E) and progesterone (P) in laying canaries to better understand how onset of incubation might regulate clutch size. Both E and P rose sharply to maxima 1 day before the first egg was laid. Thereafter, E steadily declined, but P remained high through 2 days after the first egg was laid, after which both hormones had returned to low levels. Clutch size did not explain variation in E or P output during the laying cycle. When analyzed with respect to onset of incubation, E and, to a lesser extent, P dropped significantly on the day incubation began, irrespective of whether or not females had finished ovulating. We suggest that factors initiating incubation also cause the decline in E production by small follicles, which in turn may inhibit yolk sequestration in large follicles. Further experiments in which onset of incubation is manipulated may reveal the mechanisms by which this behavior regulates clutch size and reproductive output.

Animals↗
PubMed2000

Sex steroid levels across the reproductive cycle of female leopard geckos, Eublepharis macularius, from different incubation temperatures.

Incubation temperature during embryonic development determines gonadal sex in many reptiles, including the leopard gecko (Eublepharis macularius). In this study, we examined the hormonal and behavioral changes that occur during the reproductive cycle of female leopard geckos from four (i.e., 26, 30, 32.5, and 34 degrees C) incubation temperatures. Controlling for reproductive status, plasma levels of dihydrotestosterone (DHT), testosterone (T), and progesterone (P) varied with incubation temperature but estradiol 17-beta (E2) levels did not. Controlling for the effects of incubation temperature, DHT and T levels were low when females were previtellogenic, increased slightly during early vitellogenesis, increased dramatically during late vitellogenesis (i.e., prior to ovulation), and then decreased to previtellogenic levels after ovulation. In contrast, E2 levels increased gradually from the previtellogenic stage to the early vitellogenic stage, peaked during late vitellogenesis, and decreased to previtellogenic levels after ovulation. Levels of P increased from the previtellogenic stage to the early vitellogenic stage, remained elevated during late vitellogenesis, and then decreased after ovulation. Moreover, we determined that females were not sexually receptive when previtellogenic, were somewhat receptive during early vitellogenesis (approximately 20% receptive), were most receptive during late vitellogenesis (approximately 80% receptive), and were again unreceptive after ovulation. Incubation temperature did not influence receptivity. Overall, these data show that hormone levels and behavior change coordinately during the reproductive cycle. Although incubation temperature has persistent effects on endocrine physiology in adult female leopard geckos, these effects are modest compared to hormonal changes across the reproductive cycle.

Animals↗
PubMed1983

Covalent protein binding of vinyl chloride metabolites during co-incubation of freshly isolated hepatocytes and hepatic sinusoidal cells of rats.

Proteins of isolated rat hepatic sinusoidal cells incubated with 14C-vinyl chloride, rat liver microsomes and an NADPH-regenerating system were alkylated by vinyl chloride metabolites formed by microsomes. This suggests that reactive vinyl chloride metabolites can penetrate sinusoidal cells. Protein alkylation in isolated hepatic sinusoidal cells was higher when these were co-incubated with isolated hepatocytes, indicating that reactive vinyl chloride metabolites formed by hepatocytes are stable enough to diffuse out of hepatocytes into sinusoidal cells. Glutathione added to the incubation medium inhibited the covalent protein binding of vinyl chloride metabolites in sinusoidal cells as well as in hepatocytes incubated separately and deleted the increased protein binding in sinusoidal cells co-incubated with hepatocytes. The data indicate that glutathione present in the incubation medium traps reactive vinyl chloride metabolites formed by hepatocytes which otherwise would react with cell constituents of sinusoidal cells. If similar conditions exist in vivo, the alkylation of DNA of liver endothelial cells by vinyl chloride metabolites formed in hepatocytes is possible. This would explain the induction of hemangioendotheliomas of the liver by vinyl chloride.

Alkylation↗
PubMed1983

Time-dependent alteration in endoplasmic reticulum membrane profiles during in vitro incubation of Krebs II ascites cells.

Krebs II ascites cells were harvested from the mouse peritoneum 6-8 days after inoculation and incubated in vitro in roller suspension culture for up to 22 hr. Within 2 hr of incubation a large portion of the cells entered S phase as judged by the incorporation of 3H-thymidine into DNA. The incorporation of radioactive precursors into phospholipid, protein and RNA increased rapidly during in vitro incubation indicating a high degree of macromolecular synthesis. The rates of incorporation were maximal within 4 hr of incubation. When cells labeled with 3H-choline were disrupted by nitrogen cavitation and endoplasmic reticulum (ER) membranes analyzed for subfractions on discontinuous sucrose gradients, it was observed that only small amounts of radioactivity could be detected in the HR region after 1/2 hr incubation while 63.2% of total radioactivity in ER membranes appeared in the LR fraction. Between 8-18 hr there was a considerable increase in the amount of HR membranes. Of the total radioactivity in ER membranes that in the HR fraction increased from 16.9% to 53.9% during this period. There were only small changes in the amounts of radioactivity in LR and S membranes between 8 and 18 hr. The results suggest a time-dependent appearance of ER membrane subfractions during a 22 hr period of in vitro incubation of Krebs II ascites cells.

Animals↗
PubMed1988

The effect of the incubation temperature on the cleavage rate of mouse embryos in vitro.

We have studied the effect of an elevated incubation temperature on the in vitro cleavage rate of one- and two-cell mouse embryos. Two-cell embryos demonstrated a significantly higher rate of cleavage when incubated at 39 degrees C as compared to 37 or 41 degrees C. When recovered at the one-cell stage, the difference in cleavage rate between the groups incubated at 37 and 39 degrees C did not reach significance. While the morphology of the embryos incubated at 37 degrees C did not differ from that of the embryos incubated at 39 degrees C in either group, a significantly higher rate of degeneration was noted in the group of two-cell embryos incubated at 41 degrees C. These findings may apply to the human in vitro fertilization and embryo transfer system (IVF-ET), where the existing "lag" between embryo and endometrium could be narrowed if embryo cleavage rates could be accelerated. Further documentation of the normality of these "advanced" embryos is required.

Animals↗
PubMed2001

Loading process of sugars into cabbage petiole and asparagus shoot apex cells by incubation with hypertonic sugar solutions.

The freezing tolerance of cabbage petioles and asparagus shoot apexes was increased by preincubation with 0.8 M sugar solutions. In cabbage petioles with an initial freezing tolerance of -3 degrees C (temperature for 50% cell survival), as determined by both electrolyte leakage and fluorescein diacetate vital staining, the freezing tolerance was increased to -13 degrees C by incubation with sorbitol solutions for 3 h. In meristematic cells of asparagus shoot apexes with an initial freezing tolerance of -7.5 degrees C, as determined by fluorescein diacetate vital staining, the freezing tolerance was increased to -30 degrees C by incubation with 0.8 M sugar solutions for 3 h, although other cells in the shoot apexes were killed by higher freezing temperatures. During incubation of both cabbage petioles and asparagus shoot apexes with sugar solutions, sugars were intracellularly taken up by osmotically induced fluid-phase endocytotic vesicles, as indicated by comovement of Lucifer Yellows carbohydrazide (LYCH) observed with a confocal laser scanning microscope. The amounts of intracellularly taken up sugars increased concomitantly with the formation of endocytotic vesicles depending on the time of incubation in parallel with a gradual increase of freezing tolerance. However, the endocytotic vesicles and their contents were retained not only after prolonged incubation after maximum freezing tolerance had been achieved but also after recovery of these tissue cells to isotonic conditions or after freeze-thawing. These results suggest that although sugars are intracellularly taken up by endocytotic vesicles, they might be sequestered within vesicles, casting doubt on their protective role to the plasma membranes as a main site of freezing injury. The pretreatment with 1 mM p-chloromercuribenzenesulfonic acid (PCMBS), an inhibitor of sugar transport, reduced the amounts of intracellular sugar uptake without affecting the formation of endocytotic vesicles, suggesting that sugars were, at least partly, taken up by sugar transporters. In the pretreatment with PCMBS, the freezing tolerance of incubated tissues with sugar solutions was significantly reduced, although addition of PCMBS per se did not affect survival. These results suggest that sugars taken up by sugar transporters, rather than sugars taken up by endocytotic vesicles, are mainly responsible for the increased freezing tolerance of cabbage petioles and asparagus shoot apexes. Furthermore, we aimed to study the occurrence of fluid-phase endocytosis with LYCH in an isotonic condition. Our results indicated that uptake of LYCH by fluid-phase endocytotic vesicles was not detected microscopically in isotonic condition, although LYCH was spectrofluorimetrically taken up in isotonic condition. Spectrofluorimetric uptake of LYCH was inhibited by addition of probenecid, an anion transport inhibitor. These results suggest that in cabbage petioles and asparagus shoot apexes, LYCH is taken up by anion transport but not by fluid-phase endocytosis in isotonic condition, and uptake of LYCH by fluid-phase endocytosis is restricted to occur only in hypertonic condition.

4-Chloromercuribenzenesulfonate↗
PubMed1985

Subfractionation and characterization of native and incubation enlarged human plasma high density lipoprotein particles by high performance gel filtration.

Incubation of human plasma in vitro at 37 C results in an increase of the mean particle size of the high density lipoproteins (HDL) accompanied by an almost complete disappearance of the original particles present prior to incubation. A rapid high performance gel filtration technique has been developed in order to study the chemical composition of subfractions of native and incubation enlarged HDL particles as a function of particle size. Subfractionation of HDL isolated by preparative ultracentrifugation from 3 normal human plasmas incubated in vitro at 0 and 37 C for 24 hr have been performed using a 150 cm long TSK-G 3000 SW column. The separation time was less than 65 min. The curves obtained at high performance gel filtration of HDL, by monitoring the effluents from the column at 280 nm, agreed well both in positions of peak maxima and relative peak intensities with the particle distribution patterns observed at polyacrylamide gradient gel electrophoresis of the corresponding HDL preparations run in parallel. The different HDL particle subfractions of the effluents from the gel filtration column have been characterized by quantification of free and esterified cholesterol, total phospholipids and apolipoprotein A-I and A-II. The incubation enlarged HDL particles, subfractionated by the high performance gel filtration technique, were found to have a composition which differed from that of native HDL particles of corresponding size. Incubation enlarged HDL had a generally higher and almost constant relative cholesteryl ester content over the whole particle range compared to native HDL in which a continuous increase in relative cholesteryl ester content could be observed when going from large to small particles. The molar ratio of phospholipids to free cholesterol was higher in small native HDL particles than in the corresponding large ones. The relation between apolipoprotein A-I and A-II remained nearly constant between small and large HDL particles in each subfractionation experiment. The results demonstrate that the high performance gel filtration technique is a rapid and reproducible means for studying the composition of subfractions of HDL particle populations.

Antibodies↗
PubMed2000

Plant Species and Plant Incubation Conditions Influence Variability in Epiphytic Bacterial Population Size.

The influences of plant species and plant incubation conditions on the variability in bacterial population sizes among leaves were investigated in field and growth chamber studies. Pseudomonas syringae strains TLP2 and Cit7 were inoculated onto plants and population sizes were measured at intervals after inoculation. Total bacterial population sizes were also assessed in field studies. Levels of leaf-to-leaf variability in both P. syringae population size and bacterial community size differed significantly among plant species. For all plant species, variability among leaves in population sizes of inoculated bacteria was consistently greater than the leaf-to-leaf variability in numbers of total bacteria. Considering levels of variability in population size immediately prior to and following incubation under either wet or dry physical conditions, leaf-to-leaf variability in the population sizes of inoculated P. syringae strains increased significantly following incubation under dry, but not under wet, conditions. Measurements of leaf-to-leaf variability immediately prior to and following incubation were positively correlated regardless of whether the incubation was under wet or dry conditions, though the correlation was greater following dry incubation. These data provide insight into the biological and physical factors that may be important in generating variability in bacterial population sizes among leaves, and they have important implications for the design of appropriate strategies for sampling leaf surface microbial populations. </hea

Journal Article↗
PubMed1997

[Liver metabolism during cold ischemic incubation in UW solution in the rat model].

Simple cold storage of livers for transplantation activates glycolysis due to lack of oxygen. Energy derived from glycolysis may be critical for cell survival and liver cell death may occur once glycolysis is inhibited in the liver due to accumulation of end products or lack of substrates (glycogen). The relationship between cell death (lactate dehydrogenase, LDH release), anaerobic glycolysis (lactate production), and glycogen content of liver tissue was studied during cold incubation of liver slices in UW solution. Rat livers slices from male Sprague Dawley rats were incubated at 4 degrees C in UW solution, with continuous gentle shaking, under conditions of chemical hypoxia (KCN, 5 mM). The rate of lactate production, LDH release-ATP and glycogen content were measured spectrophotometrically and by HPLC. Lactate increased nearly linearly for the first 48 h of incubation; total lactate which had accumulated after 48 h was 33.9 +/- 0.81 mumol/g and at 96 h nearly the same, 31.3 +/- 1.2 mumol/g. Glycolysis stopped, apparently, because of the depletion of liver slice glycogen which was initially 228.8 +/- 1.7 mumol/g wet wt. It decreased to 34.7 +/- 2.7 mumol/g at 48 h and to 18.7 +/- 1.1 mumol/g at 72 h and remained at this level for the next 24 h. An increased leakage of LDH occurred once glycogen metabolism (and accumulation) ceased. LDH release could be stimulated after only a few hours of cold incubation of liver tissue slices by adding glycolysis inhibitor (iodoacetic acid) to the medium. After 24 h. LDH release was 24.4 +/- 1.8% and increased to 52.8 +/- 5.2% (P < 0.05, Student's t-text) with iodoacetic acid. Adding a glycolytic substrate (fructose, 10 mM) to the medium maintained lactate production for 96 h. The stimulation of glycolysis by fructose also reduced cell death: LDH release was significantly lower at 72- and 96-h incubation (P < 0.001, two-way ANOVA). The ATP content was significantly higher with fructose (P < 0.001). Adding glucose (20 mM) and fructose (10 mM) in combination resulted in prolonged cell survival, significantly delayed glycogen depletion and significantly higher ATP content at 48 and 72 h (two-way ANOVA). Livers from rats who had fasted for 24 h demonstrated the same LDH release at 48 h when incubated with glucose (20 mM) and fructose (10 mM). In conclusion, LDH leakage from hypoxic cold-stored liver slices is related to anaerobic glycolysis. Anaerobic glycolysis appears to continue slowly under hypothermia and provides sufficient energy for maintenance of cell viability. A stimulation of glycolysis in the cold is possible by fructose and results in prolonged cell survival under hypothermic conditions. Glycogen depletion can be slowed down by combining glucose and fructose.

Adenosine↗