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PubMed1998

Heat acclimation--mechanisms of adaptation to exercise in the heat.

Repeated exposures to exercise and heat produce acclimatization, changes in physiological function by which the tolerance to heat stress is improved. The main issues to be discussed are the possible mechanisms for the increase in plasma volume, the increase in sweating rate and the endocrine responses to exercise with acclimation to both dry and humid heat. This will be discussed on the basis of the literature and our recent and ongoing experiments. We have tried to analyze this by comparing different acclimation procedures: exercise at 50-60% V/O2max, 60-90 min, dry heat 40 degrees C, 20% RH; 40-50% VO2max, 45-51 min, humid heat 35 degrees C, 85% RH; and 70-75% VO2max, 30-35 min, dry heat 40 degrees C, 20% RH. Subjects exercised in dry or humid heat for 8-12 consecutive days. Acclimation was achieved by all procedures, as indicated by a lower heart rate, increased plasma volume and sweating rate. We hypothesize that it is the repeated exposures to high core temperature that induce the changes, possibly via endocrine factors activated by the rising core temperature and the prolonged exercise. The increased sensitivity of the sweat glands for thermal and hormonal stimuli after acclimation may be obtained through an increase in receptor density for neural and humoral stimuli, an increase in the size, or, in number of active sweat glands.

Acclimatization↗

PubMed1981

Recovery of protein synthesis after heat shock: prior heat treatment affects the ability of cells to translate mRNA.

A mild heat shock at 35 degrees C, which induces heat shock gene expression, greatly enhances survival and the recovery of protein synthesis in Drosophila cells after a higher temperature heat shock. The 35 degrees C treatment is also effective in preventing heat-induced developmental defects in pupae. We show here that the major larval mRNAs are present in approximately normal (25 degrees C) concentrations after a 40.1 degrees C heat shock whether or not the animals receive a pretreatment. This indicates that the pretreatment affects translation directly rather than messenger concentration. We also observe selective translation of heat shock messages and some 25 degrees C messages during recovery from heat shock.

Animals↗

PubMed1976

Mechanisms of heat damage in proteins. 7. The significance lysine-containing isopeptides and of lanthionine in heated proteins.

1. Studies have been made with solvent-extracted chicken muscle, bovine plasma albumin (BPA) and other proteins, all severely heated in the absence of carbohydrates so as to cause a large decrease in their fluorodinitrobenzene (FDNB)-reactive lysine contents. 2. epsilon-N-(beta-L-aspartyl)-L-lysine and epsilon-N-(gamma-L-glutamyl)-L-lysine isopeptides were determined after enzymic digestion of heated chicken muscle, and their content was found to increase as the material was subjected to more heat treatment. Heated chicken muscle was not found to contain lanthionine. Heated BPA, on the other hand, was found to contain lanthionine but not the isopeptides. Both lanthionine and isopeptide cross-linkages were detected in most of the other heated proteins. There was some difficulty in quantifying the amounts of isopeptides formed on heat treatment, because the enzymic digestion procedure used in their isolation appeared to be incomplete. Neither lysinoalanine nor ornithinoalanine was detected in any of the test materials.

Alanine↗

PubMed1980

Heat loss from humans measured with a direct calorimeter and heat-flow meters.

1. Heat loss from three men and three women was measured in a direct calorimeter over 2 or 3 h periods and compared with that determined simultaneously from heat-flow meters attached to the skin surface at the waist. The comparisons were made at each of four ambient temperatures, 15, 20, 25 and 30 degrees. Each subject wore a cotton boiler-suit and minimal underwear. 2. Oral temperatures and skin and clothing temperatures on both trunk and forearm were determined, thus enabling the subjects' internal and external insulation to be calculated. 3. Heat loss determined by the meters was lower than that determined by the calorimeter. The difference increased with increase in ambient temperature. 'Meter' heat loss decreased linearly as ambient temperature was raised. 4. It was concluded that heat-flow meters could provide a useful estimate of total heat loss when the evaporative component is low. The estimate might be improved if the subject is calibrated while wearing the meters in a calorimeter over several short periods. Heat-flow meters could therefore be of particular value in sedentary individuals, when the heart-rate method for estimating energy expenditure can be inappropriate.

Adult↗

PubMed1991

Effect of step-down heating on the interaction between heat and radiation in a C3H mammary carcinoma in vivo.

The effect of step-down heating (SDH) on the interaction between heat and radiation was investigated in a C3H mammary carcinoma in vivo. SDH consisted of an initial sensitizing treatment (ST) performed at 44.5 degrees C or 43.5 degrees C followed by a lower temperature test treatment (TT) in the range 41.0-43.0 degrees C. Step-up heating (SUH), i.e. TT followed by ST, and single heating were used as controls. The end-point was the radiation dose needed to control 50% of the tumours (TCD50). The results were evaluated by calculating the thermal enhancement ratio (TER) defined as TER = TCD50 (radiation alone)/TCD50 (radiation and heat). For a simultaneous application of TT and radiation a significant enhancement of direct heat radiosensitization was observed with increasing ST time or ST temperature using SDH. In contrast, only a minor increase was seen with SUH. A comparison between TCD50 values for the corresponding SUH and SDH schedules revealed that the SDH effect was largest at 41.0-42.0 degrees C and decreased with increasing TT temperature. The radiosensitizing effect of SDH also decreased if an interval was allowed between ST and TT or between TT and radiation. However, as a result of an increased cytotoxicity towards hypoxic tumour cells, the TCD50 value for SDH remained significantly smaller than for SUH, even with a sequential combination of radiation and heat.

Animals↗

PubMed1991

Efficacy of nine heat and moisture exchangers for intraoperative airway heat conservation.

The performance of heat and moisture exchangers (HMEs) were clinically evaluated for the effectiveness of intraoperative heat conservation. Ten groups of patients were studied, nine groups with commercially available HMEs (Gibeck Humidvent 1, Terumo Brethaid, Portex Thermovent 600, NMI Pneumoist 2, Pall HME15-22, Gambro Engström Edith 1000, Gambro Engström Edith 1500, Gambro Engström Edith Flex and Siemens Servo Humidifier 153) and a control group with no HMEs. Deep temperature of the forehead (107 patients) and heat and humidity at the airway (55 patients) were measured. The temperature decrease in one hour, the total body heat loss, the temperature and absolute humidity of inspired gas and the heat loss from the airway were much better for all groups with HMEs than for the group with no HMEs, and the nine HMEs had different efficiency. There were significant correlations between the index values of body temperature and the index values of heat and humidity at the airway. In conclusion, HMEs were useful devices for preventing heat loss from the airway and maintaining body temperature.

Adult↗

PubMed1997

Airway humidification in mechanically ventilated neonates and infants: a comparative study of a heat and moisture exchanger vs. a heated humidifier using a new fast-response capacitive humidity sensor.

OBJECTIVE: To study the efficiency of a heated humidifier and a heat and moisture exchanger in mechanically ventilated neonates and infants. DESIGN: Prospective, controlled, clinical study. SETTING: University pediatric intensive care unit. PATIENTS: Forty neonates and infants who needed mechanical ventilation were enrolled in the study. INTERVENTIONS: None. MEASUREMENTS AND MAIN RESULTS: A heat and moisture exchanger and active airway humidification were alternately used in the same patients to exclude interindividual differences in airway humidification. Airway humidity was measured by a new fast-response capacitive humidity sensor which measures airway humidity with an acquisition rate of 20 Hz throughout the respiratory cycle. The humidity sensor was placed at the endotracheal tube adapter. Measurements were done at the beginning and at the end of three consecutive sessions of passive, active, and again passive airway humidification, each session lasting 6 hrs. There was no significant difference between mean inspiratory airway humidity with the heated humidifier (33.8 +/- 2.9 mg/L) and with the heat and moisture exchanger (34.0 +/- 2.6 mg/L). Moreover, the mode of airway humidification did not significantly influence body temperature or PCO2. No serious side effects such as endotracheal tube occlusion were observed. CONCLUSIONS: Passive airway humidification by a heat and moisture exchanger is effective in mechanically ventilated neonates and infants over a 6-hr period. However, the performance and safety of a heat and moisture exchanger in prolonged mechanical ventilation remain to be proven.

Costs and Cost Analysis↗

PubMed1991

Heat Shock Proteins in Two Lines of Zea mays L. That Differ in Drought and Heat Resistance.

Synthesis of heat shock proteins (HSPs) in the leaves of a drought- and heat-resistant (line ZPBL 1304), and a drought- and heat-sensitive (line ZPL 389) line of maize (Zea mays L.) was studied under two environmental stress treatments: (a) soil drying and high temperature and (b) high temperature. In the first treatment 13-day-old plants were exposed to 7-day soil drying followed by high temperature stress (45 degrees C), and in the second treatment 20-day-old plants were exposed to high temperature stress (45 degrees C). Second leaves were labeled with [(35)S]methionine. During the labeling period line ZPBL 1304 showed no signs of leaf dehydration under soil drying and high temperature stress conditions. In contrast, line ZPL 389 was dehydrated 23%, as determined by relative water content. Incorporation of [(35)S]methionine into protein was greater in the resistant than in the sensitive line in both treatments. The pattern of synthesis of HSPs in the two lines was similar in treatments 1 and 2. Both lines synthesized a high molecular mass set and a low molecular mass set of HSPs. Proteins from both sets from both lines of maize appeared similar to each other, with respect to the molecular mass. Heated plants of the drought- and heat-resistant line ZPBL 1304 synthesized a band of HSP(s) of approximately 45 kilodaltons which was not found in heated plants of the drought and heat sensitive line ZPL 389. This is the first report on qualitative intraspecific difference in the synthesis of HSPs in maize.

Journal Article↗

PubMed1989

Molecular organization of RNP complexes containing P11 antigen in heat-shocked and non-heat-shocked Drosophila cells.

Immunofluorescence analysis of polytene chromosomes of Drosophila melanogaster using the monoclonal antibody P11 has shown that after heat-shock the 38-kDa P11 antigen almost exclusively localizes at heat-shock puff 93D where it is part of giant puff-specific RNP granules. The biochemical experiments reported here show that, independent of growth temperature, the P11 antigen is a component of nuclear 10S RNP particles. The P11-containing 10S snRNPs can be stabilized in CsCl with 20 mM Mg2+ and possess a buoyant density of rho = 1.4 g/cm3. Sucrose gradient analysis of nuclear RNP extracts of heat-shocked Schneider's S-3 tissue culture cells shows that, after a 37 degree C heat-shock, the 10S RNPs associate with large RNP complexes sedimenting at 170-220S. The change in distribution is a temperature-dependent process with intermediate forms at 29 degrees C and 33 degrees C. In thermotolerant cells this observed change in distribution is strongly reduced. DEAE-Sephacel column chromatography and sucrose gradient analysis of nuclear RNP, followed by Northern blot analysis using 93D-specific probes of the TaqI repeat and immunoblotting experiments, show that the P11-containing 10S snRNPs are distinct from the RNP complexes formed by the 93D transcripts, suggesting an indirect association after heat-shock. Our experiments demonstrate that, despite the fact that a 37 degrees C heat-shock does not affect the overall integrity of nuclear RNP, it imposes changes on the general organization and interaction of the nuclear RNP population, resulting in the formation of large nuclear RNP aggregates and complexes. Such changes may be important for the survival strategy of the cell and for hnRNA processing and storage events which are effected by heat-shock.

Animals↗

PubMed1982

Frequency of Escherichia coli strains producing heat-labile toxin or heat-stable toxin or both in children with and without diarrhea in São Paulo.

Enterotoxigenic Escherichia coli strains were isolated from 32 (13.4%) of 245 children with diarrhea and from 11 (11.4%) of 96 children of the control group. Strains producing heat-labile toxin were found more frequently in normal children than in children with diarrhea. Strains producing heat-stable toxin and both heat-labile and heat-stable toxins were isolated only from children with diarrhea. Association of these strains with diarrhea was highly significant as shown by statistical analysis. The O:H types and the colonization factors of strains producing heat-stable toxin and both heat-labile and heat-stable toxins are presented.

Bacterial Toxins↗

PubMed1982

Effect of heating rate on evaporative heat loss in the microwave-exposed mouse.

Male CBA/J mice were administered heat loads of 0-28 J X g-1 at specific absorption rates (SARs) of either 47 or 93 W X kg-1 by exposure to 2,450-MHz microwave radiation at an ambient temperature of 30 degrees C while evaporative heat loss (EHL) was continuously monitored with dew-point hygrometry. At an SAR of 47 W X kg-1 a threshold heat load of 10.5 J X g-1 had to be exceeded before EHL increased. An approximate doubling of SAR to 93 W X kg-1 reduced the threshold to 5.2 J X g-1. Above threshold the slopes of the regression lines were 1.15 and 0.929 for the low- and high-SAR groups, respectively. Thus the difference in threshold and not slope attributes to the significant increase in EHL when mice are exposed at a high SAR (P less than 0.02). In separate experiments a SAR of 47 W X kg-1 raised the deep body temperature of anesthetized mice at a rate of 0.026 degrees C X s-1, whereas 93 W X kg-1 raised temperature at 0.049 degrees C X s-1. Hence the sensitivity of the EHL mode of heat dissipation is directly proportional to the rate of heat absorption and to the rate of rise in body temperature. These data contradict the notion that mammals have control over whole-body heat exchange only (i.e., thermoregulation) but instead indicate that the EHL system is highly responsive to the rate of heat absorption (i.e., temperature regulation).

Animals↗

PubMed2006

Preventive effect of egg yolk phosvitin on heat-insolubilization of egg white protein and its application to heat-induced egg white gel.

The effects of phosvitin (PV) on insolubilization of egg white protein (EWP) and ovotransferrin (OT) were examined by measuring turbidity after heating at 80 degrees C in a pH range of 5 to 8. PV showed preventive ability against heat-insolubilization of EWP, especially heat-labile OT. The preventive ability of PV was reduced by adding NaCl to a PV-OT mixture on heating. Native PAGE and gel filtration analyses showed that PV prevented an insolubilization of heat-denatured OT through ionic interactions. The preventive effects of PV on insolubilization of EWP and OT resulted in the formation of a firm, transparent gel from EWP in coexistence with PV on heating. The addition of PV might make possible the preparation of liquid egg white without insoluble products even on heat-treatment at high temperatures.

Chromatography, Gel↗

PubMed1990

[Heat distribution within the bone tissue by rotary cutting instrument for IMZ implant. Heat analysis by a real-time thermography].

A highly skilled surgical technic is an important factor for the success of implant. But the heat generation during bone preparation will greatly affect the healing process of bone and prognosis of implant. In this study, the heat occurrence and distribution during bone preparation which performed on an in vitro mandible model (ribs of pig) were considered with the aid of a real-time thermography. IMZ implant drills and bur, designed for cylindrical type implant were used for the bone preparation. The conclusion are as follows: 1. Any drill or bur generates higher heat without water irrigation. 2. Heat distribution produced different shape of range according to different type of drills. 3. On a relationship of preparating time to heat generation, spiral drill takes the longest time to complete drilling but heat rising ratio is slow. Round bur and Canon drill take short time to complete drilling but it is accompanied with rapid heat rising.

Animals↗

PubMed2006

Heat-treatment of bovine colostrum. II: effects of heating duration on pathogen viability and immunoglobulin G.

Batches (30-L) of first-milking bovine colostrum, inoculated with Mycoplasma bovis (10(8) cfu/mL), Listeria monocytogenes (10(6) cfu/mL), Escherichia coli O157:H7 (10(6) cfu/mL), Salmonella enteritidis (10(6) cfu/mL), and Mycobacterium avium subsp. paratuberculosis (Map; 10(3) cfu/mL), were heat-treated at 60 degrees C for 120 min in a commercial on-farm batch pasteurizer system. Duplicate 50-mL subsamples of colostrum were collected at 15-min intervals throughout the heat-treatment process for the purpose of bacterial culture and for measurement of IgG concentration (mg/mL) and antibody activity [log2(bovine viral diarrhea virus type 1 serum neutralization titer)]. Four replicate batches of colostrum were run for each of the 5 pathogens studied. There was no effect of heating moderate- to high-quality colostrum at 60 degrees C for at least 120 min on mean IgG concentration (pre = 60.5 mg/mL; post = 59.1 mg/mL). Similarly, there was no effect of heat-treatment on the mean log2 bovine viral diarrhea virus type 1 serum neutralization titer (pre = 12.3; post = 12.0). Viable M. bovis, L. monocytogenes, E. coli O157:H7, and S. enteritidis added to colostrum could not be detected after the colostrum was heat-treated at 60 degrees C for 30 min. Average bacteria counts showed that Map was not detected when batches were heated at 60 degrees C for 60 min. Although the authors believe that heat-treating colostrum at 60 degrees C for 60 min should be sufficient to eliminate Map from colostrum in most situations, further research is needed to determine whether these findings may be replicated, given that variability was observed in Map culture results.

Animals↗

PubMed1984

The effect of rate of heating or cooling prior to heating on tumor and normal tissue microcirculatory blood flow.

Single vessel responses to hyperthermia were studied in tumor and normal tissues using a transparent access window chamber. Rates of heating less than or equal to .68 degrees C/minute preserved relatively better vascular function in normal than tumor tissue. A rate of heating of 1.0 degrees C/minute lowered normal tissue statis temperatures so they were no different from tumor. Cooling to 30 degrees C prior to heating slowed normal arteriolar flows to less than 5% of 38 degrees C controls. Heating resulted in increased flow in those vessels, but maximum flows never exceeded 5% of flows achieved in similar vessels which were not cooled first. The implications of this work are that rate of heating and cooling prior to heating can alter normal tissue vascular response to heat in a way that could prove deleterious to maintaining efficient vascular function in that tissue relative to tumor.

Adenocarcinoma↗

PubMed1977

Effect of heat stable and heat labile Escherichia coli enterotoxins, cholera toxin and theophylline on unidirectional sodium and chloride fluxes in the proximal and distal jejunum of weanling swine.

Acute, isolated loops of proximal and distal jejunum of weanling swine were exposed to either heat stable porcine Escherichia coli enterotoxin, heat labile porcine Escherichia coli enterotoxin, cholera toxin or theophylline. Unidirectional sodium fluxes in response to heat stable in the proximal jejunum were dependent on the length of time that the intestinal mucosae was exposed to the enterotoxin. Net water, sodium and chloride and unidirectional sodium and chloride flux measurements in the proximal jejunum in response to each agent uniformly indicated that net secretion of fluid and electrolytes was the result of increased unidirectional sodium secretion or blood-to-lumen flux and decreased unidirectional chloride absorption or lumen-to-blood flux. In addition heat stable cholera toxin and theophylline but not heat labile decreased unidirectional chloride secretion a small but significant amount in the proximal jejunum. Sodium and chloride flux measurements in the distal jejunum demonstrated that all four secretory agents could stimulate net secretion of water, sodium and chloride in that region. The response to these secretory agents as measured by sodium and chloride unidirectional flux rates was not similar to changes observed in the proximal jejunum. In the distal small intestine, whereas heat labile cholera toxin and theophylline induced similar qualitative changes in unidirectional sodium and chloride fluxes, that induced by heat stable differed.

Animals↗

PubMed1988

Wear, creep, and frictional heating of femoral implant articulating surfaces and the effect on long-term performance--Part II, Friction, heating, and torque.

In Part I, (J.A. Davidson and G. Schwartz, "Wear, creep, and frictional heating of femoral implant articulating surfaces and the effect on long-term performance--Part I, A review," J. Biomed. Mater. Res., 21, 000-000 (1987) it was shown that lubrication of the artificial hip joint was complex and that long-term performance is governed by the combined wear, creep, and to a lesser extent, oxidation degradation of the articulating materials. Importantly, it was shown that a tendency for heating exists during articulation in the hip joint and that elevated temperatures can increase the wear, creep, and oxidation degradation rate of UHMWPE. The present study was performed to examine closely the propensity to generate heat during articulation in a hip joint simulator. The systems investigated were polished Co-Cr-Mo alloy articulating against UHMWPE, polished alumina ceramic against UHMWPE, and polished alumina against itself. Frictional torque was also evaluated for each system at various levels of applied loads. A walking load history was used in both the frictional heating and torque tests. The majority of tests were performed with 5 mL of water lubricant. However, the effect of various concentrations of hyaluronic acid was also evaluated. Results showed frictional heating to occur in all three systems, reaching an equilibrium after roughly 30 min articulation time. Ceramic systems showed reduced levels of heating compared to the cobalt alloy-UHMWPE system. The level of frictional torque for each system ranked similar to their respective tendencies to generate heat. Hyaluronic acid had little effect, while dry conditions and the presence of small quantities of bone cement powder in water lubricant significantly increased frictional torque.

Hip Prosthesis↗

PubMed1983

Sensitivity of xenografts of human pancreatic adenocarcinoma in nude mice to heat and heat combined with chemotherapy.

The sensitivity of two human pancreatic adenocarcinomas (Capan-1 and Capan-2) to heat and heat combined with chemotherapy was studied using xenografts of the tumors in the foot of athymic nude mice. Heat was applied by immersion of the tumor in a water bath at 43.5 degrees for 1 hr. A single i.p. dose of mitomycin C, cisplatin, 5-fluorouracil, or 0.9% NaCl solution was given at 1 hr prior to treatment. Heat treatment alone significantly suppressed tumor growth (p less than 0.001), with 35% of the tumors showing complete regression. Combined treatment using heat plus chemotherapy yielded significantly greater suppression of tumor growth (p less than 0.05) with mitomycin for both tumors and with cisplatin or 5-fluorouracil for Capan-1. Combined treatment also gave higher rates of complete tumor regression: 55 and 64%, respectively, for Capan-1 and Capan-2 as compared with 18 and 47% for the respective tumors treated by heat alone. These observations suggest that human pancreatic carcinomas are sufficiently sensitive to heat combined with chemotherapeutic treatment to warrant a clinical trial of these modalities.

Adenocarcinoma↗