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Pelvic stresses in vitro--I. Malsizing of endoprostheses.

Seven embalmed human cadaveric hemi-pelves have been stripped of all soft tissues except the articular cartilage and instrumented with 25 strain-gauge rosettes on both the medial and lateral surfaces. Loads up to 2.5 kN were applied, without simulated muscle forces, to mimic the line-of-action of the resultant joint-force in a single-legged stance. In this parametric study, endoprostheses were studied at the 'correct' size and in sizes of 1 and 2 mm above and below the correct size. The correct size of endoprosthesis produced similar periacetabular stress data to those obtained with the normal femoral head; however, significant changes in stress on the medial and lateral aspects of the ilium suggested the correctly-sized spherical endoprosthesis had a different moment arm than its anatomical counterpart. Endoprostheses of 1 mm greater than the correct size caused major increases in the magnitude of both periacetabular and medial-wall stresses, while prostheses of +2 mm created dramatic and significant increases in magnitudes of these stresses. Endoprostheses smaller than the correct size caused only small but significant changes in the pattern of stresses up to -2 mm malsizing and, in particular, were associated with the disappearance of all periacetabular tensile stresses; however, stress data on the medial and lateral aspects of the ilium were more closely matched to the 'normal' for this -2 mm of undersizing.

Hip Prosthesis↗

Pelvic stresses in vitro--II. A study of the efficacy of metal-backed acetabular prostheses.

Six embalmed human cadaveric hemi-pelves with their associated proximal femurs have been tested in vitro using 25 strain-gauge rosettes on each hemi-pelvis. Loads were applied up to 2.5 kN and principal stresses were computed from the principal strain data. Acetabular prostheses, cemented in place upon a cartilage-devoid but intact subchondral bone-plate, showed little change in stress-patterns when compared with the normal data, regardless of whether or not the component employed metal-backing. The use of 30 anchoring holes of 6.4 mm diameter, in the intact subchondral bone-plate, had little effect upon the stress-patterns, regardless of whether metal-backing was employed upon the prosthesis. When the subchondral bone-plate was removed, there were notable changes in the stress-pattern in the periacetabular region and on the medial wall of pelvis. The metal-backed prosthesis did not produce such notable changes as its plastic counterpart, when the subchondral bone-plate was removed. The use of a plastic prosthesis cemented in a Protrusio ring, in an acetabulum devoid of subchondral bone, produced notable changes in the stress-patterns in the whole periacetabular region and on the medial wall.

Acetabulum↗

Experimental study of periacetabular deformations before and after implantation of hip prostheses.

This work is based upon an experimental simulation of one-legged stance, under loads close to the average body weight. Skeletons, harvested from fresh non-embalmed cadavers, included pelvis, two lumbar vertebrae, and both femurs. Periacetabular deformations were studied using tridirectional strain gauges before and after implantation of different types of acetabular prostheses in eight pelves: conventional polyethylene-cemented sockets, polyethylene sockets cemented into a metallic device, metal-backed cemented sockets, and hemispherical cementless press-fit cups with porous or hydroxyapatite coating. Strain measurements showed the persistence of important bone deformations at implant periphery. Mean values of normal periacetabular deformations were significantly reduced in two out of five acetabula implanted with cemented implants. In contrast, mean values of normal and circumferential deformations increased when polyethylene sockets were cemented into acetabular reinforcement devices, but this augmentation was significant in only one out of two cases. After implantation of cementless hemispherical metal-backed cups, periacetabular bone deformations also increased, but modifications were significant in a single case. Results are discussed according to the type of fixation (cemented or cementless), and to the difference in rigidity between implant and bone. There was no clear relation between implant stiffness and the magnitude of bone deformations.

Acetabulum↗

Creutzfeldt-Jakob disease in an adolescent.

A 16-year-old boy was stricken with a progressive neurologic disorder characterized primarily by dementia progressing to severe neurologic debility in 12 months and death 28 months following the first symptoms. Pathologic examination showed a spongiform encephalopathy, consistent witha clinical diagnosis of Creutzfeldt-Jakob disease (CJD). The noteworthy features of the case are the age of onset, the somewhat prolonged course an the amount of white matter change. These are discussed within the frame of reference of CJD and the spongiform encephalopathies of infancy and childhood. Animal inoculation studies employing post-mortem embalmed brain as inoculum are currently in progress to determine the transmissibility of this patient's disease.

Adolescent↗

Ultrasonic non-destructive measurements of cortical bone thickness in human cadaver femur.

The knowledge of the cortical bone thickness profile in human bone has a two-fold clinical significance: to study the stress occurring in a loaded bone structure to optimize the design of prostheses; and to predict the onset of advanced bone disease such as osteoporosis. In this study, the cortical bone thickness in three embalmed human cadaver femora were measured non-destructively using an ultrasonic technique. These thickness measurements were also made using a computed tomographic (CT) scanning method. Subsequently bones were sectioned and the actual bone thicknesses in the same regions were measured using a micrometer. The correlation coefficient between the actual thickness and the ultrasonically measured thickness was 0.95 and with the CT was 0.62. Thus, these results show that, under present experimental conditions, ultrasonic thickness measurements compare well with the micrometer actual thickness results. This technique, when fully developed, can be used as a non-destructive tool for quantitative cortical bone thickness measurements. Moreover, the ultrasonic technique does not use ionizing radiation.

Bone and Bones↗

Use of uncalibrated biplanar radiography for the measurement of skeletal coordinates around the shoulder girdle.

Mechanical modelling of the musculoskeletal system is dependent upon information regarding the bony attachments of the relevant muscles; in order to study the biomechanics of the shoulder girdle the authors have identified the muscle attachments in three embalmed cadavers. A simple biplanar radiographic technique was then used to determine the attachment coordinates using frames of reference defined for each bone. This technique, using hand positioning without special fixtures was believed to be sufficiently accurate, bearing in mind the likely degree of biological variation. In order to test this assumption, the accuracy of the technique has been studied by measuring the agreement between the two measurements of the common coordinate in the pairs of radiographs. It was found that for the trunk, the errors in the common coordinate were always less than the natural variation; for the scapula they were of a similar magnitude but, for the humerus, the measurement errors frequently exceeded the variation in the coordinates of muscle attachments. It was concluded that, in general, uncalibrated biplanar radiography was sufficiently accurate for the determination of the spatial coordinates of muscle attachments.

Adult↗

The dorsal carpal ligaments: their anatomy and function.

The dorsal carpal ligaments were studied in 50 embalmed wrists. The radial collateral ligament was thin and oblique. The distribution of the dorsal radio-carpal ligament varied and it was classified into four subtypes. Neither ulnar collateral ligament nor dorsal radio-ulnar ligament was isolated as a discrete structure: instead, there were confluent soft tissues on the dorso-ulnar aspect of the wrist. The dorsal radio-carpal ligament and the dorso-ulnar component of the triangular fibro-cartilage complex, as well as the fibrous septa and the extensor tendons, were found to be the most important stabilizers of the wrist dorsally.

Humans↗

Incidence of cartilaginous and ligamentous lesions of the radio-carpal and distal radio-ulnar joint in an elderly population.

51 wrists of 30 embalmed cadavers have been used to perform an anatomical and radiological study relating cartilaginous and ligamentous lesions of the wrist with sex, age, ulnar variance (UV) and the state of the triangular fibrocartilage complex (TFCC) in an elderly population (mean 76.6 years). Two-thirds of all wrists (66%) showed cartilaginous lesions, mainly on the lunate (22, or 44%). The TFCC was perforated in 23 wrists (46%), and most were central degenerative perforations. Correlations were found between ulnar variance and TFCC thickness (P < 0.05) and ulnar variance and TFCC perforations (P < 0.05). A significant relation was observed between age and proximal row intercarpal ligamentous ruptures (P < 0.05) and between age and ulnar variance (P < 0.05). No statistical correlation was seen between ulnar variance and cartilaginous lesions on the lunate (P < 0.05) in this slightly ulnar negative population (mean-0.37 mm).

Age Factors↗

Evaluation of experimental and finite element models of synthetic and cadaveric femora for pre-clinical design-analysis.

The aim of this study was to determine the validity with which the finite element method could model synthetic bone and thereby determine the appropriateness of such femur analogues for application in pre-clinical tests. The performance of these synthetic femora was compared with cadaveric bone when employing the same geometric and material definition protocols. A four-point bend loading configuration was selected for this analysis. Four synthetic femurs and an embalmed cadaveric bone were tested experimentally to determine the structural bending stiffness (k) for the diaphysis of these bones. A finite element (FE) model was generated and an analysis performed for each bone type to estimate the Young's modulus (E) required to obtain a model stiffness equivalent to that obtained experimentally. The estimated material elastic modulus in the FE model for the synthetic femur was found to be very similar to available data for this bone analogue. The estimated cadaveric bone modulus however was found to differ significantly from documented values for cortical bone. A theoretical analysis demonstrated the great sensitivity of the estimated modulus value to the accuracy of the geometric definition. The very low variability found in the experimental test on the synthetic bones together with their more regular geometry and the possibility of achieving greater accuracy in geometric definition was shown to enable the production of a valid FE model of this bone for an isotropic homogeneous material description. Conversely, the greater irregularity of geometry, together with the less obvious differentiation between the cortical and cancellous bone in the cadaveric specimen makes accurate geometric description of this bone very difficult. This fact, together with the uncertainty concerning the quality of the cadaveric bone and its viscoelastic response during mechanical testing, makes reproduction of its behaviour in a FE model a much more demanding task. It is suggested that this greater capability of reproducing the experimental behaviour of the synthetic bone makes them a very useful model for both experimental and numerical studies which involve in-vitro pre-clinical testing of implant design and stem-bone behaviour.

Artificial Organs↗

The mobility of the sacroiliac joints in healthy volunteers between 20 and 50 years of age.

The nature and amplitude of movement in the sacroiliac joint (SIJ) is still open to controversy. Whereas some authors using modern measuring techniques have reported on the range of motion in the SIJ of patients and in embalmed elderly humans, the following is a presentation of our observations related to healthy individuals between 20 and 50 years of age. Using a three-dimensional stereophotogrammetric method, the motion in the joints of 15 males and nine females was investigated with change in posture from the upright standing position. The general description of spatial motion, as obtained through the helical axis concept, has been used. For comparison of the results obtained, the motion is also specified as components of rotation about vertical, anteroposterior and transverse axes, or in horizontal, frontal and sagittal planes respectively. The average values for total rotation and translation were low, being 1.7 degrees and 0.7 mm respectively. One of the test subjects who was known to have occasional trouble with his sacroiliac joints exhibited more than 6 degrees rotation. No statistically significant differences could be demonstrated with respect to sex, age, or parturition. RELEVANCE:--No data are available in the literature on the motion of the sacroiliac joints of healthy men and women in the age group 20-50 years. Measurements were carried out with the aid of percutaneously introduced external markers, using conventional light photography. This is a definite advantage over the use of X-rays and radio-opaque markers that would probably remain implanted in the bone indefinitely. Therefore this method might also be considered for future use in clinical research involving the mobility of the SIJ in patients.

Journal Article↗

Post-mortem digoxin levels--two unusual case reports.

Digoxin levels in post-mortem specimens are reported for two unusual cases. The blood level of digoxin in one of these cases was five times greater than any previously reported. The other case involved specimens from an embalmed body.

Autopsy↗

An unusual morphine fatality.

A case is presented of a 90-year-old white female who apparently died of natural causes in a nursing home. Subsequent investigation indicated that the decedent had been administered an unauthorized bolus of morphine just prior to her death. The embalmed body was exhumed, an autopsy performed, and specimens were collected for toxicologic analysis. The liver contained 1.5 mg/kg of free morphine as measured by radioimmunoassay. The presence of morphine was confirmed by gas chromatography/mass spectrometry. A review of the literature as well as in-house data collected from narcotic deaths clearly suggest that the morphine measured in this case was consistent with a lethal concentration. The manner of death was homicide.

Aged↗

Mechanical properties of osteopenic vertebral bodies monitored by acoustic emission.

A microdamage event, either bone microfracture or microcrack propagation, releases energy. Some of this energy is in the form of acoustic waves. We measured acoustic emission (AE) in normal and osteopenic vertebral bodies during compression loading to confirm the microdamage accumulation. The 2nd to 4th lumbar vertebrae taken from the embalmed cadavers of a 37-year-old male and a 75-year-old female were used. Bone mineral densities (BMDs) were measured by dual energy X-ray absorptiometer (DEXA). The male vertebrae had normal BMDs, while the female vertebrae had lower BMDs than the normal range and were graded osteopenic. Mechanical parameters (maximum load, maximum stress, stiffness, strain at maximum load, and apparent elastic modulus) and AE event count rates in the load-deformation curve were measured during quasi-static compression loading (deformation rate 0.1 mm/min). For all mechanical parameters, the normal vertebrae had higher values than the osteopenic vertebrae. Cumulative AE event counts until maximum load of the osteopenic vertebrae were much greater than that of normal vertebrae. The vertebrae which were well compressed to the plastic range in the load-deformation curve displayed dome-shaped fracture lines just above the end plates. These results are consistent with the hypotheses that microcracks of osteopenic vertebral bodies are generated and accumulate at lower strains than those of normal vertebrae at a specific site.

Absorptiometry, Photon↗

Insertional pattern of the inferior oblique muscle.

PURPOSE: We aimed to define the anatomy and anatomic variations of the inferior oblique muscle (IO) and classify its insertional pattern with photographs and drawings. DESIGN: Cohort study. METHODS: This study included 60 intact orbits of 30 (17 male and 13 female) embalmed adult cadavers. The inferior oblique muscle was dissected microanatomically from the origin to the insertion by using a dissection microscope. For each specimen, we photographed the insertional pattern of the muscle. A Vernier caliper was used to measure the width and length of the muscle at the insertion and from the division to the insertion, respectively. RESULTS: We classified the inferior oblique muscle into four groups according to the insertional pattern. In the first group, the muscle was formed of only one belly ("single," five eyes, 8.3%). In the second group, the muscle had a main and a secondary belly ("double," 30 eyes, 50%). In the third group, the muscle had three bellies ("triple," 16 eyes, 26.6%). In the fourth group, the muscle had more than three bellies ("multiple," 9 eyes, 15%). CONCLUSIONS: We found double, triple, and multiple muscle bellies in 91.7% of 60 cadaveric eyes. Ocular surgeons who do not frequently perform inferior oblique surgery should carefully examine the inferior temporal quadrant of the sclera to avoid missing portions of the inferior oblique muscle at the time of surgery.

Adult↗

Degradation of plastic polyoxymethylene brackets and the subsequent release of toxic formaldehyde.

PURPOSE: Heat, acids, alkalis, oxygen, abrasion, enzymes, and radiation are all viable mechanisms for the chemical breakdown of polyoxymethylene (POM), a plastic material used in some esthetic orthodontic brackets. The aim of this study was to establish the thermal characteristics of POM brackets and the chemical by-products in the as-received bracket, during thermal analyses of the bracket, and after abrasion of the slot and base of the bracket. MATERIAL: Plastic brackets and control rods made of POM were evaluated ex vivo by thermal and chemical analyses. RESULTS: POM brackets produce toxic formaldehyde gas whether heated or mechanically abraded. Patients who wear these brackets are being exposed to, at the very least, a potential irritant. Thermal analyses showed that the melting temperature of a POM bracket was approximately 178 degrees C, comparable to that for a commercial rod of POM. Both POM products started to degrade at approximately 250 degrees C, and, by 420 degrees C, both products had completely decomposed into their fundamental molecular structure, formaldehyde. A colorimetric assay with a fuchsin-aldehyde reagent (Schiff's reagent) showed that aldehydes were present in the effluent from thermal heating, from mechanical abrasion of the bracket's slot or base, and even from the as-received bracket. The only difference between the 6 to 8-week assays and the 12 to 14-week assays was the intensification of the color, which occurred because of an increase in aldehyde formation and the concomitant increase in double-bond formation. These observations are consistent with a report in the tribologic (ie, friction and wear) literature, which documented the degradation of POM when it rubs against steel. CONCLUSIONS: Because formaldehyde's inherent uses as a disinfectant base and embalming fluid preclude its beneficial presence in the human body, further use of POM for orthodontic brackets, crowns for children, and other prosthetic appliances is contraindicated because even radiography will promote its degradation.

Biodegradation, Environmental↗

Resident's ridge: assessing the cortical thickness of the lateral wall and roof of the intercondylar notch.

PURPOSE: The purpose of this study was to better delineate the anatomy of "resident's ridge," a term coined by William Clancy Jr., M.D., to describe the raised bony landmark commonly visualized just anterior to the femoral attachment of the anterior cruciate ligament (ACL). This landmark can mislead the novice surgeon into misplacing the femoral tunnel of ACL reconstructions. TYPE OF STUDY: Cadaveric anatomic study. METHODS: Ten human distal femurs harvested from embalmed specimens were fixed, sectioned, and analyzed for the presence and descriptive characteristics of resident's ridge. A single, blinded examiner evaluated slope, cortical thickness at 4 sites, and the presence or absence of a distinct ridge relative to the attachment of the ACL. RESULTS: A defined resident's ridge was present in 9 of 10 specimens. This was directly associated with a change in slope of the intracondylar roof in the same 9 of 10 patients. The mean cortical thickness at the ACL attachment site was 1.6 mm. This was thicker than at resident's ridge (mean, 0.90 mm), the cartilage-intercondylar notch junction (mean, 0.96 mm), and a point midway between the ACL attachment and the cartilage-intercondylar notch junction (mean, 0.90 mm). CONCLUSIONS: The phenomenon of "resident's ridge" is accounted for by a distinctive change in slope of the femoral notch roof that occurs just anterior to the femoral attachment of the ACL. The density change apparent at the time of notchplasty is probably caused by the transition between normal cortical thickness just anterior to the ACL and the cortical thickness of the ACL attachment. No distinctive increased cortical thickness can be identified as "resident's ridge."

Anterior Cruciate Ligament↗

Anatomic study of blood supply of the dorsum of the foot and ankle.

PURPOSE: This project was to study the different patterns of the anterior tibal and dorsalis pedis arteries in relation to the blood supply of the dorsum of the foot and ankle. METHODS: A reliable sample of 150 human embalmed cadavers was dissected. RESULTS: Four different patterns were identified. The dorsalis pedis artery was most frequently (287 cases, 95.7%) found to be the continuation of the anterior tibial artery distal to the ankle, and lay between the tendon of extensor hallucis and the first tendon of extensor digitorum longus. The other 13 cases (4.3%) showed 3 variant patterns of the anterior tibial-dorsalis pedis vascular axis: the anterior tibial artery took a more lateral course, passing in front of the lateral malleolus (6 cases, 2%); the perforating branch of the peroneal artery assumed the expected course of the dorsalis pedis artery (4 cases, 1.3%); the anterior tibal artery gave a lateral branch that replaced the perforating branch of the peroneal artery to supply the lateral aspect of the ankle (3 cases, 1%). CONCLUSION: Arterial variations of the anterior tibial-dorsalis pedis axis occurred in almost 5% of cases. CLINICAL RELEVANCE: An awareness of the existence of such variations is helpful during a preoperative assessment and could prevent injury during surgery.

Aged↗

Biomechanics of temporo-parietal skull fracture.

UNLABELLED: This paper presents an analysis of research on the biomechanics of head injury with an emphasis on the tolerance of the skull to lateral impacts. The anatomy of this region of the skull is briefly described from a biomechanical perspective. Human cadaver investigations using unembalmed and embalmed and intact and isolated specimens subjected to static and various types of dynamic loading (e.g., drop, impactor) are described. Fracture tolerances in the form of biomechanical variables such as peak force, peak acceleration, and head injury criteria are used in the presentation. Lateral impact data are compared, where possible, with other regions of the cranial vault (e.g., frontal and occipital bones) to provide a perspective on relative variations between different anatomic regions of the human skull. The importance of using appropriate instrumentation to derive injury metrics is underscored to guide future experiments. RELEVANCE: A unique advantage of human cadaver tests is the ability to obtain fundamental data for delineating the biomechanics of the structure and establishing tolerance limits. Force-deflection curves and acceleration time histories are used to derive secondary variables such as head injury criteria. These parameters have direct application in safety engineering, for example, in designing vehicular interiors for occupant protection. Differences in regional biomechanical tolerances of the human head have implications in clinical and biomechanical applications.

Acceleration↗