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At least 343 records · Page 19Linked to original sources
PubMed2005

Sunflower seed husk agar: a new medium for the differentiation of Candida dubliniensis from Candida albicans.

A sunflower (Helianthus annuus) seed husk agar medium has been developed and evaluated for differentiation of Candida dubliniensis from Candida albicans on the basis of colony morphology and chlamydospore production. All C. dubliniensis isolates (n=40) produced rough colonies with hyphal fringes and abundant chlamydospores whereas 101 of 105 (96.2%) C. albicans isolates produced smooth colonies with no evidence of chlamydospore production. Since this medium is free from oil droplets, chlamydospores can be examined with greater clarity by Dalmau plate technique. This medium provides a simple and cost-effective tool for the presumptive differentiation of C. dubliniensis from C. albicans and is particularly suited for clinical microbiology laboratories where biochemical or molecular methods for the differentiation of these two species are not available.

Agar↗
PubMed2003

Distribution of Candida species in different clinical sources in Delhi, India, and proteinase and phospholipase activity of Candida albicans isolates.

Eighty-five isolates of Candida recovered from three hundred and fifty diverse clinical sources, viz. respiratory tract (sputum, bronchial washing,bronchoalveolar lavage, tracheal aspirate), blood, urine, high vaginal swab, skin and plastic devices, were studied in detail for their morphological and biochemical characters. Seven species of Candida were identified, viz., C. albicans (45.8%), C. tropicalis (24.7%), C. parapsilosis (10.5%), C. krusei (7.0%), C. kefyr (7.0%), C. guilliermondii (3.5%), and C. glabrata (1.1%). C. albicans was the predominant species isolated from all clinical specimens, except blood from which C. krusei was most frequently (38.4%) recovered. Out of 39 isolates of C. albicans, 26 (66.6%) and 19 (48.7%) exhibited strong proteinase and phospholipase activity respectively. There was a higher prevalence of proteinase producing strains amongst the vaginal and skin isolates than that in urinary and respiratory isolates. Also a greater number of phospholipase producing strains was observed in the vaginal and urinary isolates than that in the respiratory and skin isolates.

Candida albicans↗
PubMedDate not supplied

Myeloid differentiation factor 88 (MyD88) is required for murine resistance to Candida albicans and is critically involved in Candida -induced production of cytokines.

We have studied the role of myeloid differentiation factor 88 (MyD88), the universal Toll-like receptor (TLR) adaptor protein, in murine defenses against Candida albicans. MyD88-deficient mice, experimentally infected in vivo, had a very significant impaired survival, and a higher tissue fungal burden when compared with control mice. The recruitment of neutrophils to the site of infection was also significantly diminished in MyD88-\- mice. In vitro production of proinflammatory cytokines such as TNF-alpha, IFN-gamma and IL-12p70, by antigen-stimulated splenocytes from mice intravenously infected with the low-virulence C. albicans PCA2 strain, could not be detected in MyD88-\- mice. This default of production of Th1 cytokines in MyD88-deficient mice correlated with a greatly diminished frequency of IFN-gamma-producing CD4 + T lymphocytes. Also, the frequency of IFN-gamma-producing CD8 + T lymphocytes was lower in MyD88-\- mice than in control mice. Although C. albicans-specific antibody titers in PCA2-infected mice appeared more quickly in MyD88-\- mice than in control mice, the MyD88-\- group was not able to maintain the Candida-specific IgM nor IgG titers at the third week of infection. The complexity of antigens recognized by sera from MyD88-\- mice was quite similar to that from infected control mice. Taken together, these data show that MyD88-\- mice are extremely susceptible to C. albicans infections, suggesting that MyD88-dependent signaling pathways are essential for both the innate and adaptive immune responses to C. albicans.

Adaptor Proteins, Signal Transducing↗
PubMed1990

Epidemiology of Candida infection. I. Use of killer system for typing of Candida albicans strains.

A killer system was used for epidemiological differentiating of 350 Candida albicans strains isolated from human sources of different anatomical and geographical origin. By using 9 killer strains from genera: Hansenula, Pichia, Torulopsis, Saccharomyces it was possible to differentiate 87 killer types. Our findings showed differences in the occurrence of the types according to the origin of the strains. The killer system proves to be valid for the differentiation of strains within the Candida species for epidemiological purposes.

Bacterial Typing Techniques↗
PubMedDate not supplied

[Isolation of surface antigens of Candida maltosa responsible for Candida-sensitization and their immunochemical characteristics].

Surface antigens from Candida maltosa were shown to be of heterogeneous nature while common component was present in all the preparations. These antigens were able to induce both the slow type hypersensitivity and the reaction related apparently to the later phase of immediate hypersensitivity. Hypersensitivity of slow and immediate types developed in response to invasion of living culture of C. maltosa A strain. The data obtained suggest that surface antigens from Candida might be used for production of diagnostic allergens.

Animals↗
PubMed1988

Auxotrophic heterozygosities and the ploidy of Candida parapsilosis and Candida krusei.

Evidence is presented that the type strain of Candida parapsilosis, ATCC 22019, is heterozygous MET/met and that Candida krusei Y 10930 is heterozygous URA/ura. This URA gene determines orotidine-5'-phosphate decarboxylase activity. Because each of these strains is demonstrably disomic for at least one chromosome, they are not haploid but must be diploid or aneuploid.

Candida↗
PubMed1983

[Effects of cadmium, zinc, lead and mercury on the growth and accumulating ability of Saccharomyces cerevisiae, Saccharomycopsis lipolytica, Candida tropicalis, and Candida utilis].

The effects of cadmium, zinc, lead, and mercury on the growth and the accumulating ability of Saccharomyces cerevisiae, Saccharomycopsis lipolytica, Candida tropicalis, and Candida utilis had been studied. Generally the experiments resulted in the following findings: Very small concentrations of cadmium and mercury already decrease the growth intensity of the yeasts by lengthening the lag period and the doubling time. The cell yield is not reduced. Zinc increases the productivity of Saccharomyces cerevisiae and Saccharomycopsis lipolytica by shortening the lag period. The lead concentrations investigated show no significant influence on the growth. Low pH values intensify the effectiveness of the four heavy metals. The yeasts tested accumulate heavy metals, especially cadmium, to high concentrations and therefore substrates containing heavy metals are only limited suitable for the scp-production with yeasts.

Ascomycota↗
PubMed1982

Antibacterial activities of Candida yeasts. Partial purification and characterization of the active substance of Candida guilliermondii.

The activity spectra of 25 Candida strains on strains of different species of bacteria were recorded. The active substance produced by Candida guilliermondii strain 848 was partially purified by cation exchange and chromatography gel filtration on Sephadex G-15. The substance is heat stable (80 degrees C, 10 min), not susceptible to treatment with proteases and most probably of a molecular weight smaller than 3500 Dalton. At a concentration of 50 A.U./ml it acts bacteriostatically on a Staphylococcus epidermidis strain (Fig. 1).

Anti-Bacterial Agents↗
PubMed1977

Mixed cultures of different yeasts species and yeasts with filamentous fungi in the SCP production. I. Production of single cell protein by mixed cultures Candida lipolytica and Candida tropicalis.

The aim of this study was to determine the application of mixed cultures Candida lipolytica and Candida tropicalis in the SCP production. N-paraffin fraction of crude oil and individual n-alkanes C:7--C:17 and glucose were used as carbon sources. The cultures were grown on laboratory scale in shaking flasks and in a 7 1 fermentor. It was found that the mixed cultures gave about 18% higher yield of biomass than the individual cultures.

Alkanes↗
PubMed1977

[Correlation among different Candida tropicalis, Candida rhagii and Salmonella cholerae suis 0 6,7 antigen].

The immune sera for Candida tropicalis (C. benhamii) CBS-5701, Candida tropicalis (C. vulgaris) CBS-2310 and C. rhagii (C. tropicalis v. rhagii) CBS-618 agglutinated Salmonella cholarae suis 211 (0 6,7). The immune serum for S. cholerae suis agglutinated C. tropicalis (C. benhamii), C. tropicalis (C. vulgaris) and C. rhagii (C. tropicalis v. rhagii). Absorption and agglutination cross tests demonstrated common antigen factors in the tested species and in S. cholerae suis (0 6,7).

Absorption↗
PubMedDate not supplied

[Comparative study of Candida zeylanoides and Candida vinaria].

Candida zeylanoides (Castellani) Langeron et Guerra 1920 and Candida vinaria Ohara, Nonomura et Yunome ex. M. Th. Smith 1973 were comparatively studied. The species were found to differ in the assimilation of nine carbon sources, the vitamin requirements, the resistance to elevated NaCl concentrations, the ability to utilize hydrocarbons. The ultrafine structure of the cell wall and the type of budding in C. vinaria are as in ascomycetous yeasts while the cell wall structure and the formation of buds in C. zeylanoides are as in basidiomycetous ones.

Candida↗
PubMed2004

Metschnikowia chrysoperlae sp. nov., Candida picachoensis sp. nov. and Candida pimensis sp. nov., isolated from the green lacewings Chrysoperla comanche and Chrysoperla carnea (Neuroptera: Chrysopidae).

Fourteen yeast isolates comprising three taxa were cultured from digestive tracts of adult Chrysoperla species (Neuroptera: Chrysopidae) and their eggs. The yeast taxa were distinguished based on an estimated molecular phylogeny, DNA sequences and traditional taxonomic criteria. The new yeasts are closely related to Metschnikowia pulcherrima but are sufficiently distinguished by sequence comparison of rRNA gene sequences to consider them as novel species. Here, three novel species are described and their relationships with other taxa in the Saccharomycetes are discussed. Metschnikowia chrysoperlae sp. nov. (type strain, NRRL Y-27615T = CBS 9803T) produced needle-shaped ascospores and was the only teleomorph found. Large numbers of chlamydospores similar to those observed in M. pulcherrima were also produced. The other two novel species are asexual yeasts, Candida picachoensis sp. nov. (type strain, NRRL Y-27607T = CBS 9804T) and Candida pimensis sp. nov. (type strain, NRRL Y-27619T = CBS 9805T), sister taxa of M. chrysoperlae and M. pulcherrima. A specialized relationship between yeasts and lacewing hosts may exist, because the yeasts were isolated consistently from lacewings only. Although M. chrysoperlae was isolated from eggs and adult lacewings, suggesting the possibility of vertical transmission, no yeast was isolated from larvae.

Animals↗
PubMed1994

Efficacy of escalating doses of liposomal amphotericin B (AmBisome) against hematogenous Candida lusitaniae and Candida krusei infection in neutropenic mice.

Immunosuppressed CF1 mice were infected intravenously with two strains of Candida krusei and four strains of Candida lusitaniae (two of which were resistant to amphotericin B). Mice were treated with 1 or 2 mg of amphotericin B desoxycholate per kg of body weight per day or escalating doses of liposomal amphotericin B (8 to 30 mg/kg/day) or were left untreated. Higher doses of liposomal amphotericin B were as effective as standard dose of amphotericin B desoxycholate in prolonging survival but were significantly more effective in reducing the fungal burden in the kidneys of animals infected with both C. krusei strains and the C. lusitaniae strains that were susceptible to amphotericin B desoxycholate. This advantage of liposomal amphotericin B therapy could not be demonstrated in mice infected with the C. lusitaniae strains that were resistant to amphotericin B desoxycholate.

Amphotericin B↗
PubMed2000

[Utility of random amplified polymorphic DNA in the discrimination between Candida albicans and Candida dubliniensis].

Candida dubliniensisis a recently described species closely related to Candida albicans. Since the discrimination between both species by conventional mycological methods is not easy, many researchers have been trying DNA-related techniques in order to identify C. dubliniensis correctly. In this study, we propose the use of the random amplification of polymorphic DNA (RAPD) with a commercialized short primer which discriminates between both species. This oligonucleotide, AB1-12, allowed also separating C. albicans isolates into four different genotypes. These genotypes were different from the unique genotype observed in C. dubliniensis.

English Abstract↗
PubMed1991

Candida tropicalis and Candida albicans fungemia in children with leukemia.

The records were reviewed for all patients hospitalized at a pediatric oncology center for complications of leukemia (n = 822) or lymphoma (n = 290) during an 8-year period. The results of surveillance cultures (throat, rectal, and urine) and blood cultures were analyzed to identify cases of Candida tropicalis and C. albicans colonization and/or fungemia. None of the patients with lymphoma who had positive surveillance cultures for C. albicans (n = 89) or C. tropicalis (n = 23) had fungemia. Among patients with leukemia, significant fungal infection was documented in 12 of 107 colonized with C. tropicalis (11.2%) versus 14 of 700 (2%) colonized with C. albicans (P less than 0.001). The two groups of children with fungemia were similar in primary diagnoses (predominantly acute lymphoblastic leukemia) and in the frequency of several known risk factors for infection, including the duration of neutropenia (absolute neutrophil counts, less than 500/microliters). Patients with C. tropicalis fungemia all had disseminated disease compared with nine of 14 patients with C. albicans fungemia. Also, subcutaneous abscesses were unique to patients with C. tropicalis in this series. Two patients in each group died of their infection; central nervous system involvement was present in both fatal cases of C. tropicalis fungemia. A high index of suspicion and the early institution of appropriate antifungal therapy are critical to the successful management of these infections in patients with leukemia.

Adolescent↗
PubMed1996

Candida biotypes in patients with oral leukoplakia and lichen planus. Candida biotypes in leukoplakia and lichen planus.

Prevalence of yeasts in 35 leukoplakia and 34 oral lichen planus patients was compared with that observed in persons without oral diseases. Serotype and morphotype were determined on Candida albicans isolates. Yeasts were isolated from the oral cavity specimens of 43.7% of the patients. C. albicans (serotype A) was the predominant species (76% in leukoplakia, 88.2% in lichen planus and 60.8% in healthy persons). Sixteen morphotypes were encountered on malt extract agar, being 732, 733, 734, 753 and 754 the most frequently found. Morphotypes SP1N and SP1Y were the most common on Sabouraud-trypheniltetrazolium agar (68.4% of the isolates from leukoplakia and 73.3% from lichen planus, but only 46.6% of the isolates from healthy oral mucosa showed SP1N morphotype). Presence of oral lesions was associated with a marked reduction in the yeast species and C. albicans biotypes, suggesting that C. albicans and particularly some of its biotypes, show a high potential of adaptation to the changes associated with the development of oral leukoplakia and lichen planus.

Adult↗
PubMed1992

Factors involved in the adherence of Candida albicans and Candida tropicalis to protein-adsorbed surfaces. An in vitro study using immobilized protein.

The adherence of Candida albicans and C. tropicalis to protein-adsorbed surfaces was investigated with surface-modified glass slides to which serum or salivary proteins were covalently bound. A specific adherence like a ligand-receptor interaction was observed between C. albicans and mucin- or salivary protein-immobilized glass slides. This interaction was eliminated by deglycosylation of the slides, suggesting that the receptor may be an oligosaccharide(s) contained mucin or saliva. A similar specific interaction was also observed between C. tropicalis and fibrinogen-immobilized glass surfaces. When the numbers of adherent cells to deglycosylated protein-immobilized glass glides were plotted against zeta potentials and contact angles of these protein-immobilized glass slides, a significant correlation was observed between the numbers of adherent cells and zeta potentials in the case of C. albicans (r = -0.87), whereas a significant correlation was observed between cell numbers and contact angles (r = 0.82) in the case of C. tropicalis. These results suggest that the forces governing the adherence of fungi to pellicle in dentures may vary depending upon the surface properties of fungi and substrate.

Adsorption↗
PubMed1990

Structure of the D-mannan of Candida stellatoidea IFO 1397 strain. Comparison with that of the phospho-D-mannan of Candida albicans NIH B-792 strain.

The structure of the D-mannan of Candida stellatoidea IFO 1397 strain, which has properties identical to those of the phospho-D-mannan of C. albicans serotype B strain, does not contain phosphate groups, and its 1H- and 13C-n.m.r. spectra are quite similar to those of the phospho-D-mannan of C. albicans NIH B-792 strain. However, the 1H-n.m.r. and 1H-13C-correlation n.m.r. spectra of the products obtained by digestion with alpha-D-mannosidase of C. stellatoidea D-mannan considerably differed from those of the corresponding digestion products of the C. albicans phospho-D-mannan. Additionally, the enzyme-linked immunosorbent assay, by means of a monoclonal antibody corresponding to (1----2)-linked beta-D-oligomannosyl residues, of the phospho-D-mannan of the same C. albicans strain indicated that the C. stellatoidea D-mannan does not contain any (1----2)-linked beta-D-oligomannosyl residues. The absence of these residues may be used as one of the criteria of chemotaxonomical identification of C. stellatoidea spp.

Candida↗