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PubMed2003

Visceral obesity is characterized by impaired nitric oxide-independent vasodilation.

BACKGROUND: Endothelial dysfunction has been described in obesity. This study examines the impact of visceral obesity on nitric oxide-independent relaxation in the human forearm. METHODS AND RESULTS: In ten viscerally obese and ten matched controls forearm blood flow (FBF) was measured by venous occlusion plethysmography during intrabrachial infusion of: (1) sodium nitroprusside; (2) bradykinin, before and after inhibition of vasoactive prostaglandins and nitric oxide; (3) potassium; (4) ouabain (Na(+)/K(+)ATPase inhibitor) alone or (5) in combination with BaCl(2)(K(IR)inhibitor). Baseline FBF and endothelium-independent vasodilatation were similar in the two groups. In obese patients, bradykinin-induced increase of FBF was significantly less than in controls (P<0.01). Irrespective of prostaglandins and nitric oxide inhibition, bradykinin response was lower in the viscerally obese. Intrabrachial potassium determined a significantly blunted response (P<0.05). Ouabain caused a similar, moderate decrease in basal FBF in the two groups; the coinfusion of BaCl(2)caused a more intense decline in FBF which was significantly relevant in obese (-24+/-5%, P<0.01). CONCLUSIONS: In obese patients there is a blunted nitric oxide-independent relaxation determined by a decreased response of inwardly rectifying potassium channels.

Adult↗
PubMed2000

Agonistic effects of the opioid buprenorphine on the nociceptin/OFQ receptor.

The nociceptin/orphanin FQ (N/OFQ) receptor (e.g. the human ortholog ORL1) has been shown to be pharmacologically distinct from classic opioid receptors. Recently, we have identified buprenorphine as a full ORL1 agonist using a reporter gene assay. For further functional analysis, buprenorphine's effects on ORL1 receptors were investigated using a K(+) channel (GIRK1) assay in Xenopus oocytes and GTPgammaS assay in CHO-K1 membrane preparations. In both assays, buprenorphine behaved as a partial agonist compared to nociceptin itself. The N/OFQ agonism of buprenorphine might contribute to actions of buprenorphine in pain models in vivo beside its mu- or kappa-opioid receptor mediated effects.

Analgesics, Opioid↗
PubMed2000

Direct composite inlays versus conventional composite restorations: 5-year follow-up.

OBJECTIVES: To determine at 5 year follow-up the failure rate, wear rates and other aspects of clinical performance of direct composite inlays compared with conventional composite restorations placed incrementally. METHODS: 100 matched pairs of restorations were originally entered into the trial. Each pair consisted of a direct composite inlay and a conventional composite restoration made from the same material. At 5 years it was possible to recall 65 pairs, of which 54 were complete. Clinical assessments were made using USPHS criteria (indirect measurements of occlusal wear were made using Ivoclar standard dies) and annual bite wing radiographs. RESULTS: There was a trend to more failure of inlays than conventional composites (17.4 c.f. 7.5%) but this was not significant. The clinical performance of both types of restoration was similar and compared favourably with the results of studies of other materials. Secondary decay was diagnosed in only one restoration. Between 3 and 5 years there was some deterioration in cavo-marginal discoloration, marginal adaptation (occlusally) and surface roughness (occlusally). There was no apparent deterioration in colour match, proximal contact, shim stock contacts and Gingival Index. Wear rates of both types of restoration showed no significant difference and were essentially linear with a mean of 33-34 microm per year. CONCLUSIONS: Both inlays and conventional composite restorations complied with ADA specification minimum requirements for posterior composite restorations. In this study the direct inlay technique gave no clinical advantage over conventional, incremental placement.

Adult↗
PubMed2001

Marginal quality and microleakage of adhesive class V restorations.

OBJECTIVES: The aim of this in vitro study was to determine the marginal quality and microleakage of composite resin class V restorations. METHODS: Standardized mixed class V cavities (diameter: 4mm, depth: 2mm) with half of the finish lines limited within dentin were cut in 90 freshly extracted human molars and randomly assigned to nine groups (n=10). After etching enamel and dentin, the cavities were restored with nine different restorative systems (Syntac Sprint/Tetric Ceram=SS, Syntac Single-Component/Tetric Ceram=SC, Onestep/Aeliteflo=OS, Aquaprep+Onestep/Aeliteflo=OA, Prime & Bond 2.1/TPH=PB, Optibond Solo/Prodigy=OP, Singlebond/Z100=SB, Tenure Quik/Marathon=TQ, Solobond M/Arabesk=SM) using a wet-bonding procedure. After finishing and polishing, the teeth were stored for 24h in distilled water at 37 degrees C before they were subjected to thermocycling (5/55 degrees C, 1000x). Epoxy replicas were made for margin analysis in the SEM. Specimens were stained in methylene blue, sectioned, and evaluated for microleakage. Dye penetration was scored on a 0-3 ordinal scale. RESULTS: Statistical analysis (Kruskal-Wallis H-test, Mann-Whitney U-test) revealed significant differences (P<0.05) among the groups at dentin and enamel margins for the microleakage scores as well as for the results of the quantitative SEM margin analysis. SC revealed a significantly higher percentage of perfect margins in the SEM than OS and SM in enamel and dentin, respectively. OA exhibited significantly more leakage in enamel than all other groups. CONCLUSIONS: None of the tested restorative systems achieved a perfect seal in dentin and enamel of mixed class V cavities. Marginal quality and sealing ability of adhesive systems to dentin, using a wet-bonding procedure, is still inferior compared with enamel margins.

Barium Compounds↗
PubMed2001

The effect of fluoridated and non-fluoridated rewetting agents on in vitro recurrent caries.

OBJECTIVE: This study examined the in vitro caries inhibiting potential of fluoridated and non-fluoridated rewetting agents that are applied to acid-etched enamel and dentine before the use of a water-free, dentine adhesive. MATERIALS AND METHODS: Twelve caries-free premolars were divided into three groups of four teeth each. 2 x 3 x 1.5 mm cavities were prepared on the mesial and distal surfaces of each tooth, with half of the cavosurface margin in enamel and half in root dentine. In Group I (control), One-Step (Bisco, Schaumburg, USA) was applied without etching or rewetting agents. In Group II, cavities were acid-etched, rinsed, dried, and rewetted with Aqua-Prep (Bisco), a non-fluoridated rewetting agent, and then bonded with One-Step. Treatment for Group III was similar to Group II, except that Aqua-Prep F (Bisco), a fluoridated rewetting agent was used. Bonded cavities were restored with a non-fluoride-containing flowable composite (AEliteFlo, Bisco). Artificial carious lesions were induced in these specimens, from which multiple 100+/-20 microm thick longitudinal sections were prepared, yielding 16 specimens per group for evaluation with polarised light microscopy (PLM) and microradiography (MRG). Representative sections were processed for transmission electron microscopy (TEM) examination and scanning transmission electron microscopy/energy dispersive X-ray (STEM/EDX) analyses. RESULTS: The differences in demineralisation of dentine among the groups were not statistically significant for 'relative' lesion depth (p > 0.05, ANOVA, Student-Neuman-Keuls test), but highly significant for 'relative' lesion area (p < 0.001). Wall lesions were consistently present in Group I, while inhibition zones were invariably observed in Group III. 87.5% of Group II specimens exhibited neither wall lesion nor inhibition zone. TEM showed that remnant dentine apatite crystallites within the inhibition zones in Group III were larger and denser than those present within the corresponding wall lesions. STEM/EDX analyses confirmed the presence of calcium, phosphorus and fluorine in these plate-like crystallites. CONCLUSION: When used with a water-free, single-bottle dentine adhesive, a non-fluoridated rewetting agent is able to reduce, but cannot completely prevent recurrent caries. The use of a fluoridated rewetting agent is useful under the situation when microleakage occurs, by providing the additional benefit of fluoride-induced demineralisation inhibition.

Analysis of Variance↗
PubMed2003

Volumetric shrinkage of composites using video-imaging.

OBJECTIVE: This study involves investigation of the use of video-imaging for measurement of volumetric shrinkage of composites. METHODS: Six composites were tested for volumetric shrinkage using video-imaging. The volumetric shrinkage was measured using the single- and multi-view volumetric reconstruction modes. All composites were cured using a VIP(TM) curing light for 40s at 500 mW/cm(2). Dynamic shrinkage was measured using the single-view mode with a red filter placed over the detector opening. RESULTS: Analysis of the volumetric shrinkage values by a one way ANOVA for each composite showed no difference for the single- and multi-view measurement mode. The shrinkage values determined by video-imaging were compared to those measured for the same composites by mercury dilatometry by one way ANOVA followed by a paired comparison using the Bonferroni method. CONCLUSION: The video-imaging technique gives reproducible results for volumetric shrinkage of composites comparable to those measured by dilatometry.

Acrylic Resins↗
PubMed1998

Effect of a resin-modified glass ionomer liner on volumetric polymerization shrinkage of various composites.

OBJECTIVE: The aim of this study was to evaluate the volumetric polymerization shrinkage of a selection of composite resins alone, and in contact with a resin-modified glass ionomer (RMGI) liner. METHODS: Volumetric polymerization shrinkage was measured for five different composite resins by means of the Watts and Cash deflecting disk method. Shrinkage measurements were determined for 300 s for each composite alone, and when placed over pre-cured RMGI liner. Each sample was placed within a brass ring fixed to a glass microscope slide. A linear vertical displacement transducer (LVDT) was brought into contact with a flexible glass coverslip placed over the composite sample. On curing, the contraction of the setting composite sample caused distortion of the coverslip, the resulting deflection being recorded via the LVDT by means of data-logging software. All RMGI samples were prepared at 85% relative humidity (RH) and 35 degrees C. In all cases the composite was light-cured at ambient RH and 35 degrees C. RESULTS: Analysis of the data demonstrated statistically significantly less shrinkage for each composite when cured in contact with the set RMGI liner than when cured alone, at p < 0.001. SIGNIFICANCE: Use of an RMGI liner was found to significantly reduce volumetric polymerization contraction for all the light-curing composite resin restorative materials tested.

Analysis of Variance↗
PubMed1996

The site for GTP hydrolysis on the archaeal elongation factor 2 is unmasked by aliphatic alcohols.

An appropriate mixture of ethylene glycol and BaCl2 enhanced the otherwise very low intrinsic GTPase activity of the elongation factor 2 isolated from the archaeon Sulfolobus solfataricus (SsEF-2). The enzymatic activity became up to 300-fold higher than that of the SsEF-2 GTPase measured in the absence of any stimulator, but remained 20-fold lower than that stimulated by ribosome. The stimulatory effect of ethylene glycol/Ba2+ was attributed to the increased affinity for GTP, probably related to a conformational change occurring in a hydrophobic region near the catalytic site.

Adenosine Diphosphate Ribose↗
PubMed2000

Separation of chloride and sulfate ions in univalent and divalent cation forms from aqueous streams.

The precipitation and separation of chloride and sulfate in several cation forms (sodium, potassium, magnesium, calcium, strontium, and barium) from aqueous streams were studied using isopropylamine (IPA) and ethylamine (EA) as precipitation solvents. The precipitation fractions (P) of the tested chloride salts at 5000 and 10,000 ppm by both IPA and EA over the studied range of solvents volume ratio (V(R)) were relatively identical (18-60%) and their small variations were within their experimental uncertainty. The P of combined sulfate at 1000 ppm (56-99.5%) and chloride at 5000 ppm (28-62%) in the form of calcium by IPA over the studied range of V(R) were appreciably higher than the P of sulfate (10-98.5%) from calcium sulfate in the absence of calcium chloride, or the P of chloride (18-58%) from calcium chloride in the absence of calcium sulfate. The P of chloride from oil-field-produced waters at 106,654 ppm (20-88%) by both IPA and EA were higher than the P of chloride from diluted produced water at 20,000 (17-68%) and 10,000 ppm (16-65%) over the studied range of V(R). The small amounts of sulfate present in the produced waters (e.g., 435 ppm) were completely removed at V(R) of 0.1 (the first stage of precipitation). Consistency tests performed on the acquired data indicated a good level of experimental consistency. Two model equations (2-Suffix and 3-Suffix) derived from thermodynamic principles of solid-liquid equilibrium (SLE) criteria were employed to correlate the acquired data. While both equations were adequate for correlating the precipitation data, the 3-Suffix equation was more accurate.

Algorithms↗
PubMed1997

In vitro administration of 17 beta-estradiol inhibits drug-induced contractions of the rat isolated seminal vesicle.

1. The rat isolated seminal vesicle responded to noradrenaline (NA), acetylcholine (ACh), potassium chloride (KCl) and barium chloride (BaCl2) with reproducible contractions. 2. 17 beta-estradiol (17 beta E) cumulatively added in the isolated organ bath reduced the number of contractions with all agonists used in the rank order of potency: BaCl2 > or = KCl > ACh > NA. The dose-response curves constructed in the presence of 17 beta E (2 x 10(-5) mol/l) produced a rightward shift and a reduction in the maximum response showing inhibitory activity. 3. When the calcium content in the normal Krebs medium (2.5 mmol/l) was reduced to half, the inhibitory activity of 17 beta E was potentiated. The maximum inhibition rates to KCl (phasic and tonic), BaCl2 and ACh were significantly (P < 0.05) different from each other. 4. The inhibitory effects of 17 beta E against all agonists tested were found to be similar in their responses to verapamil, but were much lower in potency. 5. The inhibitory effects of 17 beta E was seen only when the hormone was present in the tissue environment and was readily reversible as soon as the tissue was washed with the Krebs medium, suggesting that the effect of 17 beta E is localized. 6. It is suggested that the in vitro application of 17 beta E on the rat isolated seminal vesicle interferes with the process of translocation of calcium ions from the extracellular medium.

Acetylcholine↗
PubMed1997

Carbon dioxide regulates the tonic activity of locus coeruleus neurons by modulating a proton- and polyamine-sensitive inward rectifier potassium current.

The electrophysiological effects of CO2 on locus coeruleus noradrenergic neurons were investigated in rat brain slices. Under control conditions, when slices were perfused with artificial cerebrospinal fluid containing 24 mM NaHCO3/5% CO2 (pH approximately 7.34, 33 degrees C) and exposed to 5% CO2/95% O2 arriving through an interface chamber, locus coeruleus neurons discharged spontaneously at approximately 1 Hz. Extracellular recordings showed that lowering CO2 that arrived through the chamber below 5% resulted in reductions in firing rate, often with a complete cessation of activity when exogenous CO2 was removed completely. Intracellular recordings revealed that lowering CO2 produced an outward current with an increase in slope conductance and a reversal potential near the potassium equilibrium potential; doubling the concentration of external potassium shifted the reversal potential of the current activated by CO2 removal by approximately +20 mV. Raising CO2 above 5% induced an increase in firing rate, an inward current, a decreased slope conductance at potentials near resting membrane voltage, and an increased slope conductance at more negative potentials. These effects of CO2 were mimicked by other manipulations that are known to affect intracellular pH. For example, NH4Cl, which acutely induces intracellular alkalinization, caused a marked reduction in firing rate, an outward current and an increased slope conductance that reversed near the potassium equilibrium potential. Bath-applied barium blocked the effects induced by removal of CO2 or addition of NH4Cl. The polyamine spermine (tetrahydrochloride) applied via intracellular micropipettes blocked the outward current induced by removal of CO2 or addition of NH4Cl. Spermine (free base) or an equivalent concentration of putrescine failed to alter the CO2 (0%)- or NH4Cl-induced effects. We conclude that CO2 maintains the tonic activity of locus coeruleus neurons by decreasing intracellular pH which, in turn, closes inward rectifier potassium channels, an effect that may be mediated by a protonated polyamine. According to this model, when there is alkalinization of locus coeruleus cells through removal of CO2 or addition of NH4Cl, endogenous spermine or a similar polyamine becomes partially deprotonated, releasing the channel block and allowing the cell to hyperpolarize. The possible implications of these results for the physiological effects of CO2 in the locus coeruleus are discussed.

Ammonium Chloride↗
PubMed1999

Hyperpolarization-activated inward potassium and calcium-sensitive chloride currents in beating pacemaker insect neurosecretory cells (dorsal unpaired median neurons).

Hyperpolarization-activated inward currents were studied in single adult cockroach Periplaneta americana pacemaker neurosecretory cells, identified as dorsal unpaired median neurons using the whole-cell patch-clamp technique. Under current clamp, injection of negative current produced a hyperpolarization of the cell membrane with a sag in the membrane potential toward the resting value. Under voltage clamp, the whole-cell current-voltage relationship exhibited an unexpected biphasic aspect. The global hyperpolarization-activated inward current could be dissociated by means of 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonic acid and tetraethylammonium chloride sensitivity, ionic selectivity, voltage dependence and activation threshold as inward potassium and calcium-sensitive chloride currents. The inward potassium current was activated around -80 mV. The reversal potential followed the potassium equilibrium potential when the extracellular potassium concentration was raised. This current was not dependent on the external sodium concentration and was sensitive to 10 mM tetraethylammonium chloride or 5 mM barium chloride. The hyperpolarization-activated inward calcium-sensitive chloride current was activated in a range of potential 20 mV more positive than the potassium current. The estimated reversal potential (-71 mV) was very close to the equilibrium potential for chloride ions ( 73 mV). Intracellularly applied 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonic acid, 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid and external application of 1 mM zinc chloride, calcium-free saline or high concentrations of intracellular 1,2-bis-(2-aminophenoxy)ethane-N,N,N',N'-tetra-acetate blocked the inward chloride current. Current-clamp experiments indicated that the inward potassium current accounted for inward rectification of dorsal unpaired median neurons. Our findings report, for the first time in pacemaker neurosecretory cells, the co-existence of two distinct hyperpolarization-activated inward currents which have specialized function in pacemaker activity.

4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid↗
PubMed2001

Suicidal poisoning with barium chloride.

A 49-year-old male pharmacist suffering from depression phoned the emergency services telling of how he had ingested barium chloride. He was found semicomatose in bed and resuscitation attempts were to no avail and he died at the scene. A white plastic container labelled "Barium chloride... Poison", and a book with a writing on a blank page... "give sulphate... SO(4)" were found. At autopsy, 1l of whitish-yellow fluid was found in the stomach. Autopsy barium levels were: blood 9.9mg/l; bile 8.8mg/l; urine 6.3mg/l; gastric 10.0g/l. Cause of death was given as cardiorespiratory arrest due to barium chloride poisoning. The issue of barium toxicity in a variety of itatrogenic and non itatrogenic situation is discussed together with the two only other cases of suicidal barium ingestion, and the feasibility of early intervention at the scene by an emergency team.

Autopsy↗
PubMed1997

Thrombogenicity of small-diameter prosthetic grafts: relative contributions of graft-associated thrombin and factor Xa.

PURPOSE: We evaluated the contributions of coagulation factors IIa (thrombin) and Xa to small-diameter prosthetic graft thrombogenicity in vivo. METHODS: Preclotted and nonpreclotted (collagen-coated) polyester grafts were studied before and 24 hours after implantation into pig femoral arteries. After incubation of explanted grafts was performed with plasma depleted of vitamin K-dependent coagulation factors by barium chloride adsorption (Ba-plasma), graft-associated thrombin activity was determined by radioimmunoassay for fibrinopeptide A. Fibrinopeptide A levels reflect thrombin-mediated fibrin formation. Factor Xa activity was characterized by measuring activation of prothrombin added to Ba-plasma. RESULTS: Thrombin and factor Xa were associated with the luminal surfaces of preclotted grafts before and 24 hours after implantation. Nonpreclotted grafts had negligible procoagulant activity before implantation. After 24 hours in vivo graft-associated factor Xa activity was similar in both nonpreclotted and preclotted grafts; however, more thrombin was bound to nonpreclotted coated grafts (p < 0.01). CONCLUSIONS: The procoagulant activity of small-diameter prosthetic grafts persists for 24 hours after implantation and is attributable not only to graft-associated thrombin but also to de novo thrombin elaboration induced by factor Xa. Moreover, graft-associated procoagulant activity is not dependent on preclotting because it develops on nonpreclotted, collagen-coated grafts as well. Treatment strategies to attenuate graft thrombosis may require the inhibition of both thrombin and factor Xa.

Adsorption↗
PubMed1998

Alcohol-induced vascular damage of brain is ameliorated by administration of magnesium.

Ethanol ingestion can cause irreversible neuronal and vascular damage in the brain and stroke-like events. Using an intact in vivo rat brain (pial) model, TV image-intensification, cultured cerebral vascular muscle cells, digital-image analysis, and a novel Mg2+ ion-selective electrode to measure extracellular ionized Mg2+, studies were designed to determine whether: 1) perivascular or systemic administration (i.v. or intra-arterial) of magnesium aspartate HCI (MgA) exert vasodilator effects on arterioles (65-130 microm o.d.) and venules (60-135 microm); 2) nonvasodilator doses of MgA could modify vascular spasms induced by BaCl2 and ethanol; 3) nonvasodilator doses of MgA could ameliorate or prevent the cerebral vascular damage induced by high doses of ethanol; and 4) ethanol depletes cerebral vascular muscle of intracellular Mg ions ([Mg2+]i). Perivascular application of MgA (0.01-100 micromol) produced dose-dependent vasodilatation of cerebral arterioles and venules; arterioles yielded greater vasodilator responses compared to venules. Nonvasodilator doses of Mg (1.0, 4.0 micromol/min), administered i.v. or intra-arterially, into a branch of the internal carotid artery, prevented: 1) the spasmogenic actions of ethanol and Ba2+; and 2) the vasculotoxic actions (rupture of postcapillary venules and focal hemorrhages) of ethanol. In addition, ethanol depleted cerebral vascular muscle cells of [Mg2+]i; blood levels of ionized Mg2+ rose after IP ethanol. Despite the fact that systemic infusion of low nonvasodilator doses did not result in dilatation of the pial arterioles and venules, plasma total and ionized Mg rose 18-230%, depending upon dose of MgA and time of plasma sampling. These data support the idea that Mg2+ can act as a local vasodilator on brain microvessels and possess antispasmodic properties on brain arterioles and venules. In addition, our results indicate that Mg may possess some unique cerebral vascular protective properties against the vasculotoxic effects of ethanol. Lastly, these findings suggest ethanol-induced cerebrovasospasm and vascular damage appear to be associated with a rapid loss of [Mg2+]i from cerebral vascular muscle cells.

Animals↗
PubMed2002

Healthcare system factors and colorectal cancer screening.

BACKGROUND: Developing effective programs to promote colorectal cancer (CRC) screening requires understanding of the effect of healthcare system factors on access to screening and adherence to guidelines. METHODS: This study assessed the role of insurance status, type of plan, the frequency of preventive health visits, and provider recommendation on utilization of CRC screening tests using a cross-sectional, random-digit-dial survey of 1002 Massachusetts residents aged > or =50. RESULTS: A broad definition of CRC screening status included colonoscopy or barium enema (screening or diagnostic) within 10 years, flexible sigmoidoscopy (FSIG) within 5 years, and fecal occult blood testing (FOBT) in the past year as options; 51.7% of subjects aged 50 to 64 and 61.5% of older subjects were current. The uninsured had the lowest current testing rate. Among insured participants, type of insurance had little impact on CRC testing; older subjects enrolled in HMOs had marginally higher rates, although not statistically significant. Increased frequency of preventive health visits and ever receiving a physician's recommendation for FSIG or ever receiving FOBT cards were associated with higher rates of CRC screening among both age groups. CONCLUSIONS: Even when broad criteria are used to define current CRC screening status, a substantial proportion of the age-eligible population remains underscreened. Obtaining regular preventive care and receiving a physician's recommendation for screening appear to be potent facilitators of screening that should be considered in designing promotional efforts.

Aged↗
PubMed1993

[Voluntary barium poisoning].

A 45-year-old man attempted to commit suicide by ingesting a large amount of barium. In some hours, he experienced generalized muscle weakness with hypokalaemia, treated by large dose of potassium (440 mmol in the first day). This weakness resulted in difficulties in swallowing and respiratory failure requiring mechanical ventilation. An anuric renal insufficiency started early, requiring haemodialysis for three weeks. It was induced probably by renal toxicity of barium and recovered completely. Later, the patient experienced an extrapyramidal syndrome initiated by tremor and myoclonia. Hypertonia induced a parkinsonian rheumatism, fixing the two hands in an irreducible position. There was also a contracture of superior sphincter of oesophagus, with severe disturbance of deglutition, ending after three months only. MRI study showed a bilateral hypersignal in basal ganglia and thalamus. It remains unknown whether this neurological syndrome was toxic or ischaemic. This patient remained under mechanical ventilation for three months because of disturbances of deglutition. He was discharged to his home at the 6th month. One year later he was still adynamic, but able to carry our rather precise movements.

Acute Kidney Injury↗
PubMed2000

Tryptophan fluorescence quenching by alkaline earth metal cations in deionized bacteriorhodopsin.

Tryptophan quenching by the addition of alkaline earth metal cations to deionized bacteriorhodopsin suspensions was determined. The results show that the addition of cation primarily quenches fluorescence from surface tryptophan residues. The quenched intensity exhibits a 1/R dependence, where R is the ionic radius of the corresponding metal ion. This observation results from a stronger energy transfer coupling between the tryptophan and the retinal. The membrane curvature may be involved as a result of cations motion and correlated conformational changes.

Bacteriorhodopsins↗