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At least 343 records · Page 19Linked to original sources
PubMed2002

Comparison of histomorphometric descriptors of bone architecture with dual-energy X-ray absorptiometry for assessing bone loss in the orchidectomized rat.

We have compared different methods for measuring bone loss in the orchidectomized (ORX) rat model of male osteoporosis: densitometry (DXA), ash weight, anatomic bone indices, histomorphometry and two-dimensional trabecular architecture analysis. Forty-eight male Wistar rats were studied at 2, 4, 8 and 16 weeks (four groups). In each group, 6 rats were ORX and 6 sham-operated were used as control. DXA was performed on the whole body, tibia and femur. Histomorphometry was performed on the secondary spongiosa of the tibia: trabecular bone volume (BV/TV) and trabecular characteristics (number, separation and thickness) were measured. Architecture analysis comprised strut identification, star volume of the marrow spaces and trabeculae, Euler-Poincaré number (E) and Kolmogorov fractal dimension (Dk). Bone mineral densities of the whole body, tibia and femur were reduced at 16 weeks in the ORX group. BV/TV was significantly decreased in the ORX group from the fourth week. Differences in the sensitivity of the architectural methods were found. There were no differences in trabecular thickness nor in trabecular star volume between ORX and controls even after 16 weeks. E became different at 8 weeks. Trabecular number, node count, star volume of the marrow spaces and trabecular separation became significantly different at 4 weeks Dk was modified after 2 weeks (p < 0.05 at 2 weeks, p < 0.001 from 4 weeks). In the ORX model, Dk appeared the most potent descriptor of trabecular bone disorganization by revealing the earliest changes at the network level.

Absorptiometry, Photon↗
PubMed2005

Acute administration of the novel serotonin and noradrenaline reuptake inhibitor, S33005, markedly modifies sleep-wake cycle architecture in the rat.

RATIONALE: The interrelationship between depressive states and sleep-wake cycle architecture is characterised by a decreased latency to the first paradoxical sleep (PS) episode, together with an enhancement of PS during the first part of the night. Conversely, slow-wave sleep (SWS) is decreased and intermittent awakenings increased. Notably, antidepressant treatment is generally associated with a diminution of PS. OBJECTIVES: In light of these observations, we examined the influence of acute administration of the novel mixed serotonin-noradrenaline reuptake blocker, (-)1-(1-dimethylaminomethyl 5-methoxybenzocyclobutan-1-yl)-cyclohexanol HCl (S33005), upon sleep-wake architecture in rats. METHODS: Animals were injected with vehicle or incremental doses of S33005 at the onset of either the dark or light periods. Digitised polygraphic recordings were performed, and changes evoked by S33005 were determined over 24-h recording periods, i.e., number and duration of sleep-wake episodes, latencies to PS and SWS, power band spectra of the electroencephalogram (EEG) and circadian changes. RESULTS: At 0.04 mg/kg, S33005 was inactive, whereas at 0.63 mg/kg, it modestly increased PS latencies and diminished PS duration during the light period. At 10 mg/kg, S33005 reduced markedly PS duration for about 4-h when injected prior to both light and dark periods. Latency to PS was prolonged, and the circadian acrophase was delayed. These effects are in keeping with previous studies of monoamine reuptake inhibitors, but, notably, SWS duration was increased when S33005 was injected at the onset of the light phase (+4%). These changes occurred without marked modifications in circadian rhythmicity or EEG spectral band power. Finally, even at the highest dose of S33005, only a limited rebound of SWS (+5%) and PS (+10%) was apparent. Amongst antidepressant to date examined, this is an original profile of influence upon sleep patterns. CONCLUSIONS: These results demonstrate a pattern of influence of S33005 upon sleep-wake architecture in rats which is globally consistent with antidepressant properties, but with a distinctive enhancement of restorative slow-wave sleep.

Adrenergic Agonists↗
PubMed2006

Selection for chromosome architecture in bacteria.

Bacterial chromosomes are immense polymers whose faithful replication and segregation are crucial to cell survival. The ability of proteins such as FtsK to move unidirectionally toward the replication terminus, and direct DNA translocation into the appropriate daughter cell during cell division, requires that bacterial genomes maintain an architecture for the orderly replication and segregation of chromosomes. We suggest that proteins that locate the replication terminus exploit strand-biased sequences that are overrepresented on one DNA strand, and that selection increases with decreased distance to the replication terminus. We report a generalized method for detecting these architecture imparting sequences (AIMS) and have identified AIMS in nearly all bacterial genomes. Their increased abundance on leading strands and decreased abundance on lagging strands toward replication termini are not the result of changes in mutational bias; rather, they reflect a gradient of long-term positive selection for AIMS. The maintenance of the pattern of AIMS across the genomes of related bacteria independent of their positions within individual genes suggests a well-conserved role in genome biology. The stable gradient of AIMS abundance from replication origin to terminus suggests that the replicore acts as a target of selection, where selection for chromosome architecture results in the maintenance of gene order and in the lack of high-frequency DNA inversion within replicores.

Base Sequence↗
PubMed2003

Relationship between muscle architectural features and oxygenation status determined by near infrared device.

This study tested whether regional differences in oxygenation status could result from differences in muscle fiber architecture. Architectural properties, oxygen supply, and consumption in the medial head of the gastrocnemius muscle (GM) were determined in vivo in six men using B-mode ultrasound and functional near infrared (NIR) imaging devices. Fascicle length, fascicle angle, NIR-O(2) saturation (deoxygenated Hb or oxygenated Hb), and NIR-blood volume (sum of deoxygenated and oxygenated Hb) were obtained in the distal and proximal portions of the GM at rest and during contraction. Exercise consisted of 2 min of standing plantar flexion at 1 Hz with an additional load of 50% of each subject's weight. Plantar flexion produced larger decreases (Delta: difference between rest and exercise values) in NIR-O(2) saturation [mean saturation (SD) of 0.14 (0.05) vs 0.07 (0.04) optical density units] and NIR-blood volume [mean saturation (SD) of -0.23 (0.08) vs -0.13 (0.04) optical density units] in the distal compared with the proximal portion ( P<0.05 for all comparisons). It also produced larger changes (Delta) in fascicle length [mean length (SD) of -16.5 (4.7) vs -8.2 (4.2) mm] and fascicle angle (mean angle (SD) of 10.8 (1.4) degrees vs 3.9 (2.1) degrees ] in the distal compared with the proximal portion ( P<0.05 for all comparisons). There were significant correlations between DeltaNIR-O(2 )saturation and Deltafascicle length ( r=-0.84, P<0.05), and between DeltaNIR-O(2) saturation and Deltafascicle angle ( r=-0.90, P<0.05), between DeltaNIR-blood volume and Deltafascicle length ( r=0.91, P<0.05), between DeltaNIR-blood volume and Deltafascicle angle ( r=-0.85, P<0.05). In conclusion, the plantar flexion exercise produced regional differences in oxygenation status consistent with regional differences in muscle architecture.

Adult↗
PubMed2000

Sonographic studies of human soleus and gastrocnemius muscle architecture: gender variability.

The purpose of this study was to establish if there are gender differences in muscle architecture in relaxed human soleus and gastrocnemius muscles of normal, live subjects. Ultrasonography was used to measure fiber bundle length, muscle thickness, and angles of pennation in a total of ten predetermined sites in the medial and lateral heads of gastrocnemius and the anterior and posterior soleus in 19 males and 16 females. Percentage differences between males and females for each parameter were recorded. Gender differences were statistically analyzed using multivariate analysis of variance. In the gastrocnemius and soleus muscles of males and females the differences between the overall fiber bundle length, angle of pennation and muscle thickness were statistically significant (P < 0.05). Overall, females were found to have longer average muscle fiber bundle length and males thicker muscles and larger angles of pennation. The greatest percentage differences of the architectural parameters between males and females were in the posterior soleus: 13% difference in fiber length and 26% difference in angle of pennation in the midline of posterior soleus and 26% difference in muscle thickness of the lateral part of posterior soleus. No correlation was found between leg length and fiber length, angle of pennation or muscle thickness. Fiber length (decreased), angle of pennation (greater) and muscle thickness (greater) of most parts of the gastrocnemius and soleus muscles were significantly different in males and females. Leg length of males and females did not correlate to these architectural parameters.

Adult↗
PubMed1998

Forming independent components via temporal locking of reconstruction architectures: a functional model of the hippocampus.

The assumption is made that the formulation of relations as independent components (IC) is a main feature of computations accomplished by the brain. Further, it is assumed that memory traces made of non-orthonormal ICs make use of feedback architectures to form internal representations. Feedback then leads to delays, and delays in cortical processing form an obstacle to this relational processing. The problem of delay compensation is formulated as a speed-field tracking task and is solved by a novel control architecture. It is shown that in addition to delay compensation the control architecture can also shape long-term memories to hold independent components if a two-phase operation mode is assumed. Features such as a trisynaptic loop and a recurrent collateral structure at the second stage of that loop emerge in a natural fashion. Based on these properties a functional model of the hippocampal loop is constructed.

Animals↗
PubMed2000

Role of architecture in determining passive electrical properties in gap junction-connected cells.

The electrical properties of gap junction-connected cells were analysed in terms of their architectural organization. Two major architectural categories were considered: trees and rings. Trees are described by means of Bethe lattices (lattices with no rings) with arbitrary co-ordination and rings by two-dimensional periodic lattices with fourfold (square) or sixfold (triangular) co-ordination. The Bethe lattice is solved analytically by the transfer constant method, which allows the introduction of several physiologically relevant effects in a very simple manner. The experimental data for the length constant and the input resistance were fitted by varying the coupling and membrane resistances for various morphologies. The large variations in the length constant observed experimentally in two systems (turtle retina horizontal cells with and without dopamine and pancreatic beta-cells in the active and silent phases) could not be explained by means of the Bethe lattice, indicating that the cell arrangements form rings. Subsequent analysis by means of a linear chain and the square and triangular lattices showed the crucial relevance of architecture in deriving the electrical characteristics of gap junction-connected cells from experimental data.

Animals↗
PubMed2005

Quantitative architectural analysis: a new approach to cortical mapping.

Recent progress in anatomical and functional MRI has revived the demand for a reliable, topographic map of the human cerebral cortex. Till date, interpretations of specific activations found in functional imaging studies and their topographical analysis in a spatial reference system are, often, still based on classical architectonic maps. The most commonly used reference atlas is that of Brodmann and his successors, despite its severe inherent drawbacks. One obvious weakness in traditional, architectural mapping is the subjective nature of localising borders between cortical areas, by means of a purely visual, microscopical examination of histological specimens. To overcome this limitation, more objective, quantitative mapping procedures have been established in the past years. The quantification of the neocortical, laminar pattern by defining intensity line profiles across the cortical layers, has a long tradition. During the last years, this method has been extended to enable a reliable, reproducible mapping of the cortex based on image analysis and multivariate statistics. Methodological approaches to such algorithm-based, cortical mapping were published for various architectural modalities. In our contribution, principles of algorithm-based mapping are described for cyto- and receptorarchitecture. In a cytoarchitectural parcellation of the human auditory cortex, using a sliding window procedure, the classical areal pattern of the human superior temporal gyrus was modified by a replacing of Brodmann's areas 41, 42, 22 and parts of area 21, with a novel, more detailed map. An extension and optimisation of the sliding window procedure to the specific requirements of receptorarchitectonic mapping, is also described using the macaque central sulcus and adjacent superior parietal lobule as a second, biologically independent example. Algorithm-based mapping procedures, however, are not limited to these two architectural modalities, but can be applied to all images in which a laminar cortical pattern can be detected and quantified, e.g. myeloarchitectonic and in vivo high resolution MR imaging. Defining cortical borders, based on changes in cortical lamination in high resolution, in vivo structural MR images will result in a rapid increase of our knowledge on the structural parcellation of the human cerebral cortex.

Algorithms↗
PubMed2003

Impact of two fluorescent pseudomonads and an arbuscular mycorrhizal fungus on tomato plant growth, root architecture and P acquisition.

The ability of fluorescent pseudomonads and arbuscular mycorrhizal fungi (AMF) to promote plant growth is well documented but knowledge of the impact of pseudomonad-mycorrhiza mixed inocula on root architecture is scanty. In the present work, growth and root architecture of tomato plants (Lycopersicon esculentum Mill. cv. Guadalete), inoculated or not with Pseudomonas fluorescens 92rk and P190r and/or the AMF Glomus mosseae BEG12, were evaluated by measuring shoot and root fresh weight and by analysing morphometric parameters of the root system. The influence of the microorganisms on phosphorus (P) acquisition was assayed as total P accumulated in leaves of plants inoculated or not with the three microorganisms. The two bacterial strains and the AMF, alone or in combination, promoted plant growth. P. fluorescens 92rk and G. mosseae BEG12 when co-inoculated had a synergistic effect on root fresh weight. Moreover, co-inoculation of the three microorganisms synergistically increased plant growth compared with singly inoculated plants. Both the fluorescent pseudomonads and the myco-symbiont, depending on the inoculum combination, strongly affected root architecture. P. fluorescens 92rk increased mycorrhizal colonization, suggesting that this strain is a mycorrhization helper bacterium. Finally, the bacterial strains and the AMF, alone or in combination, improved plant mineral nutrition by increasing leaf P content. These results support the potential use of fluorescent pseudomonads and AMF as mixed inoculants for tomato and suggest that improved tomato growth could be related to the increase in P acquisition.

Fungi↗
PubMed2005

Effects of combined elcatonin and alendronate treatment on the architecture and strength of bone in ovariectomized rats.

We examined the combined effects of elcatonin (ECT) and alendronate (ALN) on bone mass, architecture, and strength in ovariectomized (OVX) rats. Fifty female Sprague Dawley rats, aged 13 weeks, were divided into Sham, OVX, OVX+ECT, OVX+ALN, and OVX+ECT+ALN groups (n = 10). Immediately after ovariectomy, ECT was administered at a dose of 15 units (U)/kg three times a week, and ALN was administered daily at a dose of 2.0 microg/kg, subcutaneously for 12 weeks. The three-dimensional architecture of the bone in the distal femoral metaphysis was analyzed using a microfocus X-ray computed tomography system (microCT), and bone strength was measured using a material-testing machine. Trabecular bone volume (BV/TV) and number (Tb.N) were significantly greater in the OVX+ECT and OVX+ALN groups than in the OVX group. In the OVX+ECT+ALN group, BV/TV and Tb.N were significantly greater when compared with those in the OVX+ECT and OVX+ALN groups. Trabecular thickness (Tb.Th) was significantly greater in the OVX+ECT+ALN group than in the OVX+ALN group. With regard to bone strength, the compression strength in the femoral metaphysis was significantly lower in the OVX group than in the Sham group. The reduction of compression strength was slightly lower in the OVX+ECT and OVX+ALN groups. In the OVX+ECT+ALN group, the compression strength in the femoral metaphysis significantly increased when compared with the OVX and OVX+ECT groups. These results suggest that the combined treatment of ECT and ALN does not alter the individual effects of each drug and that it exerts an additive effect on trabecular architecture and bone strength in OVX rats.

Alendronate↗
PubMed2006

Tumor microvascular architecture phenotype (T-MAP) as a new concept for studies of angiogenesis and oncology.

Heterogeneities exist in endothelial cells and microvascular architecture during tumor angiogenesis. We found significantly variable expression profiles of EC markers, including ephrinB2 and its receptor EphB4, and various types of the architecture. We propose a new concept of tumor microvascular architecture phenotype (T-MAP), reflecting the density, morphology, structure and the three-dimensional distribution of newly formed vessels. The pattern of T-MAP may represent the invasiveness of the tumor therefore predict the outcome of therapy. The presence of T-MAP heterogeneity (T-MAPH) may be utilized as additional diagnostic criteria and for therapeutic designs for antiangiogenesis.

Brain Neoplasms↗
PubMed2005

The plant architecture of rice (Oryza sativa).

Plant architecture, a collection of the important agronomic traits that determine grain production in rice, is mainly affected by factors including tillering, plant height and panicle morphology. Recently, significant progress has been made in isolating and collecting of mutants that are defective in rice plant architecture. Although our understanding of the molecular mechanisms that control rice tillering, panicle development and plant height are still limited, new findings have begun to emerge. This review, therefore, summarizes the recent progress in exploring the mechanisms that control rice plant architecture.

Gene Expression Regulation, Developmental↗
PubMed2000

Influence of Low-Flow Oxygen Supply on Sleep Architecture in Patients with Severe Heart Failure (NYHA III-IV) and Cheyne-Stokes Respiration.

Patients suffering from severe heart failure may develop breathing pattern disorders during sleep, especially in the form of Cheyne-Stokes respiration. Results may be severe disturbances in sleep architecture and worsening of hemodynamics and of prognosis of these patients. Causes of the periodic breathing disorders are probably hypocapnia, hypersensitivity of respiratory control centers, hypoxemia, and prolonged blood circulation time. This study examined the influence of different concentrations of continously administered oxygen during the nighttime on breathing pattern disorders, oxygen saturation, and sleep architecture in 65 patients with severe heart failure (NYHA III-IV). Fifty-two of 65 patients showed an improvement of sleep architecture. Total sleeping time increased significantly (p < 0.01). Fragmentations of sleep by arousals decreased ( p < 0.01); time of random eye movement (REM) sleep and non-REM sleep III and IV increased significantly.

Journal Article↗
PubMed1997

A colonic tissue architecture assay applied to human colon carcinoma cells.

A two-component tissue architecture assay system has been devised that tests the ability of human colon carcinoma cells to conform to the specific three-dimensional cell-cell and cell-substratum interactions characteristic of normal colonic tissues. Dissociated fetal rat colonic cells (FRCC) were allowed to reaggregate in suspension with or without the addition of different proportions (0.1%, 1%, and 10% of the total cells) of the human colon carcinoma cell lines, SW-1222 and LS-174T. Cellular aggregates obtained after 36 hours, incubation exhibited cell sorting by the formation of recognizable epithelial colonic crypt-like structures with glandular lumens in a mesenchyme-like background. Carcinoembryonic antigen (CEA)-positive SW-1222 cells in 10% mixed aggregates were organized into numerous well-formed glandular structures with a polarized apical distribution of CEA. LS-174T cells, on the other hand, were self-sorted but structurally disorganized with a continuous cell surface CEA distribution. Pure FRCC and mixed aggregates were implanted under the kidney capsules of Swiss nu/nu (nude) or CD-1 nu/nu mice and allowed to grow for a period of 7-10 days. Whereas the normal FRCC readily formed colonic tissue, the SW-1222 cells exhibited a capacity for differentiation into colonic crypts which became progressively less normal and more tumor-like as the proportion of carcinoma cells in the aggregates was increased. The LS-174T cells demonstrated poor differentiation at all concentrations. Cell surface levels of CEA and the CEA family member nonspecific crossreacting antigen (NCA), both overexpressed in colon cancer, were higher in LS-174T than in SW-1222 cells, whereas family member biliary glycoprotein (BGP), downregulated in colon carcinoma was higher in the SW-1222 cells. These results thus support the suggestion that deregulated expression of CEA family members can be involved in the ability of colonocytes to differentiate and conform to normal tissue architecture as assessed by the assay. The assay is therefore amenable to genetic analysis of normal and perturbed architectural phenotypes.

Animals↗
PubMed1994

Preserved features of thalamocortical projection neuron dendritic architecture in the somatosensory thalamus of the rat, cat and macaque.

A number of studies have shown that the organization of the mammalian somatosensory thalamus varies between species. As differences in cellular and synaptic thalamic organization would be expected to influence neuronal dendritic architecture, we compared somatosensory thalamocortical projection (TCP) neurons from the rat, cat and macaque. The results show that key features of the dendritic branching pattern remain unchanged despite large differences in the size of TCP neurons between the species. The features examined were: (i) ratio of the length of terminal branches to the length of the entire dendritic tree; (ii) the percentage of branch points that gave rise to two daughter branches as opposed to those that gave rise to three or more daughter branches; (iii) the proportional sum of absolute deviations (a measure of branching symmetry), and (iv) the mean branch order of the terminal segments. The present study provides evidence that somatosensory TCP neurons in these species comprise a homogeneous class and share a common dendritic architecture that is conserved across species despite changes in other aspects of thalamic circuitry. This suggests that TCP neuronal form is based on relatively stable genetic blueprint and that epigenetic factors (e.g. synaptic input) resulting from evolutionary changes in thalamic organization have had less influence on dendritic architecture.

Animals↗
PubMed1996

A new clinical laboratory information system architecture from the OpenLabs project offering advanced services for laboratory staff and users.

The OpenLabs project aims to improve the efficiency and effectiveness of clinical laboratory services by integrating decision support systems with laboratory information systems and equipment. Standards for electronic data interchange between laboratories and other medical systems using the EUCLIDES/OpenLabs coding scheme and an open architecture for clinical laboratory information systems have been specified. This article gives an account of the proposed architecture and outlines new software applications being developed using the architecture which provide advanced services for ordering and reporting of laboratory tests, advanced instrument workstation and laboratory management services, including an OpenLabs Service Manager application which co-ordinates the available services.

Chemistry, Clinical↗
PubMed1987

A therapy planning architecture that combines decision theory and artificial intelligence techniques.

Through our experience with the ONCOCIN cancer therapy consultation system, we have identified a set of medical planning problems to which no single existing computer-based reasoning technique readily applies. In response to the need for automated assistance with this class of problems, we have devised a computer program called ONYX that combines decision-theoretic and artificial intelligence approaches to planning. We discuss our rationale for devising a new planning architecture and describe in detail how that architecture is implemented. The program's planning process consists of three steps: (i) the use of rules derived from therapy planning strategies to generate a small set of plausible plans, (ii) the use of knowledge about the structure and behavior of the human body to create simulations that predict possible consequences of each plan for the patient, and (iii) the use of decision theory to rank the plans according to how well the results of each simulation meet the treatment goals. This architecture explicitly manages the uncertainty inherent in many planning tasks, introduces a possible mechanism for the dissemination of decision-theoretic therapy advice, and potentially increases the number of problem solving domains in which expert system techniques can be effectively applied.

Artificial Intelligence↗
PubMed1991

Muscle architecture in relation to function.

Animal muscles generate forces and induce movements at desirable rates. These roles are interactive and must be considered together. Performance of the organism and survival of the species also involve potential optimization of control and of energy consumption. Further, individual variability arising partly via ontogeny and partly from phylogenetic history often has pronounced and sometime conflicting effects on structures and their uses. Hence, animal bodies are generally adequate for their tasks rather than being elegantly matched to them. For muscle, matching to role is reflected at all levels of muscular organization, from the nature of the sarcoplasm and contractile filaments to architectural arrangements of the parts and whole of organs. Vertebrate muscles are often analyzed by mapping their placement and then "explaining" this on the basis of currently observed roles. A recent alternative asks the obverse; given a mass of tissue that may be developed and maintained at a particular cost, what predictions do physical principles permit about its placement. Three architectural patterns that deserve discussion are the classical arrangement of fibers in pinnate patterns, the more recent assumption of sarcomere equivalence, and the issue of compartmentation. All have potential functional implications. 1. The assumption of equivalence of the sarcomeres of motor units allows predictions of the fiber length between sites of origin and insertion. In musculoskeletal systems that induce rotation, the observed (but not the pinnation-associated) insertion angle will differ with the radial lines on which the fibers insert. In a dynamic contraction inducing rotation, a shift of moment arm has no effect for muscles of equal mass. 2. Classical pinnate muscles contain many relatively short fibers positioned in parallel but at an angle to the whole muscle, reducing the per fiber force contribution. However, the total physiological cross-section and total muscle force are thus increased relative to arrangements with fibers parallel to the whole muscle. Equivalent muscles may be placed in various volumetric configurations matching other demands of the organism. The loss of fiber force due to (pinnate, not equivalent) angulation is compensated for by the reduced shortening of fibers in multipinnate arrays. 3. Compartmentation, i.e., the subdivision of muscles into independently controlled, spatially discrete volumes, is likely ubiquitous. Differential activation of the columns of radial arrays may facilitate change of vector and with this of function. Compartmentation is apt to be particularly important in strap muscles with short fiber architecture; their motor units generally occupy columnar, rather than transversely stacked, subdivisions; this may affect recovery from fiber atrophy and degeneration.(ABSTRACT TRUNCATED AT 400 WORDS)

Animals↗