PubMed1999
Transplantable tumors of the thyroid gland have been produced by feeding of thiouracil (TU) to inbred Fischer 344 rats followed by the transplantation, initially, of pieces of hyperplastic thyroid gland, and in later generations, of pieces of transplanted tissue into similar rats or into rats fed a high iodine diet. In early generations, transplants grew only in the rats fed the TU diet, and this tissue was called dependent, whereas if the tissue grew in rats fed the high iodine diet in the absence of TU, it was called independent. Dependent tumors were, initially, either papillary or of follicles distended with colloid. Later generations of some sublines were cellular or microfollicular in pattern and some became progressively more heterogeneous with later generations. Independent tumors began to appear by the third transplant generation. They were, initially, relatively uniform in pattern, and some tended to remain so, whereas other sublines exhibited some heterogeneity. Tumors had patterns that were cellular, or microfollicular, or follicular or had open follicles, etc.; there was one cellular ascites tumor subline. Other observations were made of vascular patterns, connective tissue, necrosis, and metastases.