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PubMed1993

Mixed segregation and recombination of chromosomes and YACs during single-division meiosis in spo13 strains of Saccharomyces cerevisiae.

Diploid yeast strains, homozygous for the mutation spo13, undergo a single-division meiosis and form dyads (two spores held together in one ascus). Dyad analysis of spo13/spo13 strains with centromere-linked markers on five different chromosomes and on a pair of human DNA YACs shows that: (a) in spo13 meiosis, chromosomes undergo mixed segregation, namely some chromosomes segregate reductionally whereas others, in the same cell, segregate equationally; (b) different chromosomes exhibit different segregation tendencies; (c) recombination between homologous chromosomes might not determine that a bivalent undergoes reductional rather than equational segregation.

Cell Division↗
PubMed1996

Effects of homology, size and exchange of the meiotic segregation of model chromosomes in Saccharomyces cerevisiae.

In most eukaryotic organisms, chiasmata, the connections formed between homologous chromosomes as a consequence of crossing over, are important for ensuring that the homologues move away from each other at meiosis I. Some organisms have the capacity to partition the rare homologues that have failed to experience reciprocal recombination. The yeast Saccharomyces cerevisiae is able to correctly partition achiasmate homologues with low fidelity by a mechanism that is largely unknown. It is possible to test which parameters affect the ability of achiasmate chromosomes to segregate by constructing strains that will have three achiasmate chromosomes at the time of meiosis. The meiotic partitioning of these chromosomes can be monitored to determine which ones segregate away from each other at meiosis I. This approach was used to test the influence of homologous yeast DNA sequences, recombination intiation sites, chromosome size and crossing over on the meiotic segregation of the model chromosomes. Chromosome size had no effect on achiasmate segregation. The influence of homologous yeast sequences on the segregation of noncrossover model chromosomes was negligible. In meioses in which two of the three model chromosomes experienced a crossover, they nearly always disjoined at meiosis I.

Chromosome Mapping↗
PubMed1987

A comparative examination of the residential segregation of persons 65 to 74 and persons 75 and above in 18 United States metropolitan areas for 1970 and 1980.

Indexes of dissimilarity were calculated for 18 standard metropolitan statistical areas in 1970 and 1980. The indexes measured the level of residential segregation of persons 65 and older, 65 to 74 and 75 and older. Results indicated a significantly higher level of segregation for persons 75 and older than persons 65 to 74 in both 1970 and 1980. The difference in the level of residential segregation between persons 65 to 74 and 75 and older significantly increased from 1970 to 1980. When measured as a single age group aged 65 and above, there was no significant change in the level of residential segregation from 1970 to 1980. This result indicates that failure to disaggregate the elderly population into two distinct age groups would have resulted in missing changes in the level and trend of residential segregation among the elderly population.

Aged↗
PubMedDate not supplied

Simple segregation analysis: a review of its history and terminology.

The history of simple segregation analysis is reviewed with the aim of clarifying the present confusion in terminology. Although Weinberg's contribution to simple segregation analysis is considerable, it is noted that he was not responsible for one of the methods often attributed to him. For the benefit of biologists attempting to understand simple segregation analysis, the various names given by different authors to some of the methods of segregation analysis that are still in use are incorporated into a table. All the confusion in terminology can be avoided, however, by using an extension (due to Davie) of the singles method that combines a very simple and efficient estimate of segregation frequency with a very quick and simple estimate of its variance, and that is valid for all levels of ascertainment.

Animals↗
PubMedDate not supplied

Racial residential segregation: a fundamental cause of racial disparities in health.

Racial residential segregation is a fundamental cause of racial disparities in health. The physical separation of the races by enforced residence in certain areas is an institutional mechanism of racism that was designed to protect whites from social interaction with blacks. Despite the absence of supportive legal statutes, the degree of residential segregation remains extremely high for most African Americans in the United States. The authors review evidence that suggests that segregation is a primary cause of racial differences in socioeconomic status (SES) by determining access to education and employment opportunities. SES in turn remains a fundamental cause of racial differences in health. Segregation also creates conditions inimical to health in the social and physical environment. The authors conclude that effective efforts to eliminate racial disparities in health must seriously confront segregation and its pervasive consequences.

Black or African American↗
PubMed1997

Bulked segregant analysis using microsatellites: mapping of the dominant white locus in the chicken.

In order to perform a linkage study, the genotypes of a large number of individuals from a segregating population need to be determined. In case the phenotype to be mapped is influenced by a single locus or a major gene, sampling of the DNA from individual animals with the same phenotype into a single pool (bulked segregant) can reduce the number of typings. In this study we used bulked segregant analysis in order to map the Dominant White locus in the chicken. In a pilot experiment, we showed that allele frequencies can be accurately estimated from pooled samples using fluorescently labeled microsatellite markers. A segregating population for the Dominant White locus was obtained by performing a cross between a white male chicken (Genotype li for Dominant White) and a black female chicken (ii). The resulting progeny of 21 white and 18 black chickens were divided in two pools. Genotypes for both the parents and the pools were determined using 168 fluorescently labeled microsatellite markers, of which 68 were informative. The relative allele frequencies between the pools were estimated for these 68 informative markers. One marker (MCW188) was found to segregate with the Dominant White locus. Subsequent typing of all individuals from this cross and an additional 148 animals from five different families showed only two recombinants between the marker and the Dominant White locus, resulting in a LODlinkage score (log10 of odds) of 36. Using the pooled DNA approach, the Dominant White locus was successfully mapped on linkage group 22 of the East Lansing reference family at a distance of 2 cM from MCW188.

Animals↗
PubMed2004

Seasonal sexual segregation in two Thalassarche albatross species: competitive exclusion, reproductive role specialization or foraging niche divergence?

Sexual segregation by micro- or macrohabitat is common in birds, and usually attributed to size-mediated dominance and exclusion of females by larger males, trophic niche divergence or reproductive role specialization. Our study of black-browed albatrosses, Thalassarche melanophrys, and grey-headed albatrosses, T. chrysostoma, revealed an exceptional degree of sexual segregation during incubation, with largely mutually exclusive core foraging ranges for each sex in both species. Spatial segregation was not apparent during brood-guard or post-guard chick rearing, when adults are constrained to feed close to colonies, providing no evidence for dominance-related competitive exclusion at the macrohabitat level. A comprehensive morphometric comparison indicated considerable species and sexual dimorphism in wing area and wing loading that corresponded, both within and between species, to broad-scale habitat preferences relating to wind strength. We suggest that seasonal sexual segregation in these two species is attributable to niche divergence mediated by differences in flight performance. Such sexual segregation may also have implications for conservation in relation to sex-specific overlap with commercial fisheries.

Animals↗
PubMed1979

Genetic instability of sporulation-associated characters in a Bacillus subtilis mutant: relationship between sporulation, segregation and the synthesis of extracellular enzymes (kinetic studies).

In the genetically unstable, protease-overproducing 'medusa (M) strains of Bacillus subtilis, segregation of stable, wild-type-like B cells occurred mainly during sporulation. After the end of the exponential growth phase, a small fraction of M cells sporulated quickly and formed M spores, while the majority of the cells, after a 'critical period', gave rise to B segregants which sporulated after a delay. Segregation occurred without cell division. Delayed sporulation, segregation and protease overproduction are related. Similar but more complex results were obtained with the highly unstable TD strains. Sporulation and the kinetics of protease overproduction were also followed in several stable segregants. Depending on the strain, either the rate of protease production or both the rate and time course were affected. The results are interpreted in terms of sequential activation and de-activation of sporulation genes. The production of the alkaline and the neutral proteases was, in general, under common genetic control. In some strains alpha-amylase was also overproduced.

Alleles↗
PubMed1980

Patterns of cell polarity and chromosome segregation in chains of sporulating Bacillus megaterium.

The asymmetry of the DNA duplex due to polynucleotide strand complementarity could be the molecular basis of cell polarity in spore-forming bacteria. To test this possibility, the relationship of DNA strand segregation to the spore location pattern in chains of sporangia was investigated in Bacillus megaterium. Spores containing one chromosomes labelled in one of the complementary strands were formed from cells that had been allowed to segregate pulse-labelled chromosomes in minimal medium at 30 degree C. A second crop of spores was then formed from cells originating from the labelled spore population. The second generation spores inherited labelled strands from the first sporepopulation by random segregation. In contrast, the patterns of spore positions in sporangial chains were non-random. Furthermore, the non-randomness of patterning was stable and was unaffected by growth temperature (15 to 37 degrees C) or by enrichment of the minimal medium used in the segregation experiments. Since the pattern of DNA strand segregation is random and the spore location pattern in chains of sporangia is non-random, the asymmetry of the DNA duplex cannot be the determinant of cell polarity.

Bacillus megaterium↗
PubMed1991

Vacuolar segregation to the bud of Saccharomyces cerevisiae: an analysis of morphology and timing in the cell cycle.

Vacuoles of Saccharomyces cerevisiae were visualized by phase-contrast microscopy. Visualization was enhanced by adding polyvinylpyrrolidone. Vacuolar segregation during the cell cycle was analysed in 42 individual cells of strain X2180 by time-lapse photomicrography. Within 15 min of bud emergence, more than 80% of the cells contained a vacuolar segregation structure in the form of either a tubule or an alignment of vesicles. The structure emerged from one point of the mother vacuole, then elongated and moved into the bud in a few minutes. The vacuolar segregation structure disappeared, usually within 20 min, before nuclear migration, leaving a separate vacuole in the bud. To test the generality of this observation several strains were grown in the presence of the vacuolar vital dye fluorescein isothiocyanate. The bud size was used to measure progress in the cell cycle. All strains formed vacuolar segregation structures in cells with small buds, although with variations in duration and timing in the cell cycle. In the presence of nocodazole vacuolar segregation occurred normally, thus, microtubules seem not to be essential in this process.

Cell Cycle↗
PubMed1999

Colony morphotypes on Congo red agar segregate along species and drug susceptibility lines in the Mycobacterium avium-intracellulare complex.

Isolates of the Mycobacterium avium-intracellulare complex (MAC) have long been known to segregate into transparent opaque and rough colony morphotypes that differ from each other in clinically important parameters including drug susceptibility and virulence. Here the authors report additional morphotypic variation that occurs on two levels: interspecific (between M. avium and M. intracellulare) and intraspecific (within individual M. avium isolates). Clinical isolates of M. avium grown on Congo red (CR) plates formed red, pink or mixed (red and white) opaque colonies, while M. intracellulare isolates formed purely white opaque colonies. A quantitative CR binding assay showed that this interspecific differential applies to transparent as well as opaque colony variants; however, it was less pronounced among laboratory reference strains than among recent clinical isolates. Opaque colonies of M. avium isolates with 'mixed' phenotypes segregated into stable opaque red and white variants with shared IS1245 banding patterns (intraspecific segregation). White segregants of M. avium were more flocculent and significantly more resistant to ciprofloxacin and rifamycin drugs than were red segregants. Thus, cultivation on CR agar revealed a previously unknown multidrug resistant colony morphotype of M. avium.

Anti-Bacterial Agents↗
PubMed2002

Simulational studies of axial granular segregation in a rotating cylinder.

Discrete particle simulation methods have been used to study axial segregation in a horizontal rotating cylinder that is partially filled with a mixture of two different kinds of granular particles. Under suitable conditions segregation was found to occur, with the particles separating into a series of bands perpendicular to the axis. In certain cases the band structure exhibited time-dependent behavior, including band formation, merging and motion along the axis, all corresponding to phenomena that arise experimentally. In order to examine how the many parameters specifying the problem affect the segregation process, simulation runs were carried out using a variety of parameter settings, including combinations of friction coefficients not realizable experimentally. Both segregation and desegregation (mixing) were investigated, and cylinders with both explicit end caps and periodic ends were used to help isolate the causes of segregation.

Journal Article↗
PubMed2003

Competition between interchain and intrachain phase segregation.

Single-molecule observations of giant DNA have clarified that individual molecules undergo a marked discrete transition between an elongated coil state and a compact globule state. There is a relatively wide region of coexistence between the coil and the globule states, i.e., interchain phase segregation, with a change in intensive variables such as the concentration of the condensing agent, salt concentration, temperature. Very recently, the coexistence of coil and globule conformations within a single long DNA chain, i.e., intrachain phase segregation, has been reported under certain experimental conditions. In this study, we investigated general conditions for intrachain phase segregation in a single polyelectrolyte molecule, based on a simple statistical model. We consider the contribution of condensed counterions and the interaction energy of a charged coiled region. Intrachain phase segregation is stable with regard to free energy within a suitable parameter region. Our results suggest that intrachain phase segregation occurs when the electrostatic screening effect by the salt solution is negligible or when the screening effect is large and there is attractive interaction between polyelectrolyte segments.

Biophysical Phenomena↗
PubMed2003

Inertial particle segregation by turbulence.

We study collections of heavy and light small spherical particles initially well mixed with each other, subjected to linear (Stokes) drag force and gravity, and falling through a fluid turbulence. We introduce the segregation power spectrum, which we use to define the segregation length scale. Kinematic simulation predicts that the turbulence can segregate heavy and light falling particles and leads to a well-defined segregation length scale. The properties of this length scale and of the segregation power spectrum used to define it are discussed and, where possible, explained.

Journal Article↗
PubMed2003

Role of friction in compaction and segregation of granular materials.

We investigate the role of friction in compaction and segregation of granular materials by combining Edwards' thermodynamic hypothesis with a simple mechanical model and mean-field based geometrical calculations. Systems of single species with large friction coefficients are found to compact less. Binary mixtures of grains differing in frictional properties are found to segregate at high compactivities, contrary to granular mixtures differing in size, which segregate at low compactivities. A phase diagram for segregation versus friction coefficients of the two species is generated. Finally, the characteristics of segregation are related directly to the volume fraction without the explicit use of the yet unclear notion of compactivity.

Journal Article↗
PubMed2003

Competition between chaos and order: mixing and segregation in a spherical tumbler.

We investigate the competition between granular mixing and segregation in a sphere rotating and rocking on two orthogonal axes. Operation corresponds to the continuous-flow regime and the flow within the sphere is three-dimensional and time-periodic. Experimental results are organized in a frequency/amplitude phase diagram showing modes of segregation (band formation/no axial bands); segregated bands are remarkably robust and survive rocking amplitudes as large as 60 degrees over a wide range of frequencies. Details differ, but the phenomenon occurs under both dry and slurry conditions, that is, when all air is replaced by a liquid. Experimental space-time plots of the stationary segregated patterns agree well with Poincaré maps obtained using a continuum model of the flow, suggesting that the final segregation patterns are relatively independent of materials tumbled.

Journal Article↗
PubMed2004

Sensitivity of granular segregation of mixtures in quasi-two-dimensional fluidized layers.

Size segregation is studied using a quasi-two-dimensional rotating cylinder system for mixtures of different size, near-spherical particles. Flow occurs only in a thin surface layer, whereas the remaining particles rotate as a fixed bed. In most of the systems studied, the measured radial weight fraction profiles in the bed show significant double segregation (a core of small particles as well as a thin layer of small particles at the periphery). The profiles are found to be sensitively dependent on the surface roughness of the particles in the mixture, and double segregation reduces with particle roughness. Double segregation is also sensitive to cylinder diameter and no double segregation is observed for the smaller diameter cylinders used. The system, however, shows two unexpected scalings: (i). the scaled profiles are nearly the same for different cylinder diameters, when the cylinder diameter to the cylinder length ratio is the same, and (ii). the profiles obtained are found to be insensitive to the size of the large particles in the mixture but depend strongly on the size of the small particles.

Journal Article↗
PubMed1985

Preliminary evidence of segregation distortion in the SLA system.

The segregation of 11 well-defined SLA haplotypes was investigated in 40 Land-race and 48 Large White Danish and Swiss litters. Among the 11 haplotypes, the segregation of one (SLA 20-8.2.11) deviated significantly from the expected 1:1 segregation ratio in back-cross families. Tests indicated that these families were homogeneous with respect to segregation distortion, although the distortion was more pronounced in litters sired by heterozygous Danish boars than by heterozygous Swiss boars and Danish and Swiss sows. The data presented do not allow any definite conclusion about the cause of the segregation distortion. The possibility of the distortion being caused either by a complex similar to the T/t-complex found in mouse and contemplated in man or directly by the SLA region is discussed.

Animals↗