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At least 325 records · Page 18Linked to original sources
PubMed1981

Cerebrospinal fluid IgE and the diagnosis of cerebral cysticercosis.

Cerebral cysticercosis (CC) is a common cause of seizures and other central nervous system disorders in the Mexican-American population. The elevation of serum IgE in many parasitic diseases led us to study CSF and serum IgE in patients with CC diagnosed by pathologic, radiologic or clinical criteria. A paper disc radioimmunoassay, sensitive to 0.01 IU/ml, was used to measure CSF and serum IgE in 69 patients. The mean CSF IgE level in Mexicans with CC was 0.44 IU/ml compared to 0.28 IU/ml in Mexicans without CC and 0.07 IU/ml in non-Mexicans without CC.

Brain Diseases↗
PubMed1981

A simple and rapid solid-phase radioimmunoassay for serum progesterone, using the protein A of Staphylococcus aureus as immunoadsorbent.

A simple, rapid, and inexpensive radioimmunoassay method for serum progesterone is described, which uses a solid-phase technique for separation of antibody-bound from antibody-free progesterone. Rabbit antiprogesterone immunoglobulins are adsorbed on the protein A of formaldehyde- and heat-treated Staphylococcus aureus cells (Pansorbin; Calbiochem-Behring Corp., La Jolla, Calif.). The suspension of antibody-coated pansorbin retains all its binding activity (23% for 500 microliter of a 0.033% suspension) of 1-2-H(N)-progesterone (20,000 cpm in 100 microliter) when kept at +4 degrees or at -25 degrees C for at least 4 months. Dose-response curves obtained with ether-serum extracts and with the progesterone standard do not deviate significantly from parallelism. The progesterone standard gives identical dose-response curves whether diluted in the assay buffer or in a progesterone-free ether-serum extract. The sensitivity of the assay is 0.02 ng/assay tube. The intra-assay variation coefficient is 16%, and the routine interassay variation coefficient is 17%. The mean serum progesterone concentrations were 0.55 ng/ml during the follicular phase of the menstrual cycle and 12.5 ng/ml during the luteal phase. The average blank value for distilled water was 0.02 ng/assay tube.

Adolescent↗
PubMedDate not supplied

[Immunoglobulin levels at different ages in the healthy child population].

In this study, we report the IgE, IgA, IgM and IgG levels in the serum and the secretory IgA obtained from a group of children of different ages with normal morphgram, with no personal or family history of allergies and without recurrent infectious diseases. The IgG, IgA and IgM and salivary secretory IgA were determined by the immunonephelometry technique, using specific anti-serum for heavy chains obtained form rabbits and purified by affinitive chromatography. The IgE was determined by radioimmunoassay, using the PRIST technique. If we look at all published works about normal levels of immunoglobulins in serum, we can see many differences among the results obtained by different authors. Our results are different as well; however, there is no difference in IgE levels. In our work, the secretory IgA level cannot be compared as we do not have any other study available for comparison. We think that our results differ from those obtained by other authors because of differences in laboratory and statistical techniques and other factors such as environment, constitution, nutrition, etc.

Age Factors↗
PubMed1980

[Differences in the levels of serum immunoglobulin E determined by the competitive radio-immuno-sorbent and the paper-radioimmuno-sorbent methods: Removal of these differences by pretreatment of sera with polyethylene glycol (author's transl)].

The serum IgE of patients with chronic nonspecific lung diseases was determined with the competitive radio-immuno-sorbent and the paper-radio-immuno-sorbent methods (Phadebas). Some samples showed markedly higher values with the competitive radio-immuno-sorbent than with the paper-radio-immuno-sorbent method. These discrepancies were abolished by pretreatment of the sera: polyethylene glycol 6000 (final concentration 35 g/l) was added, followed by centrifugation at 0 degrees C for 30 min at 20 000 g, and IgE was determined in the supernatant. This treatment had no effect on the values of the paper-radio-immuno-sorbent method, but the values of the competitive radio-immuno-sorbent method were decreased so that the two values agreed within the experimental confidence limits.

Binding, Competitive↗
PubMed1980

Demonstration and quantification oomycosis.

Using a radioimmunoassay technique, we have determined the total IgE concentration and specific IgE antibody level against Paracoccidioides brasiliensis in the sera of patients with mycologically confirmed active paracoccidioidcosis. Patients with aspergilloma, or individuals with antibodies against Cadida albicans, and sera of apparently healthy individuals from the endemic area were also examined. The level of total IgE was significantly elevated in 9 out of 10 cases of paracoccidioidomycosis. The mean level of specific IgE against P. brasiliensis was significantly higher in the serum of the patients in comparison with control individuals. The percentage of specific IgE relative to the total varied from 0.38 to 2.28%. The differences between IgE levels in pare other mycoses were statistically significant. Our results suggest a possible involvement of specific IgE antibodies against P. brasiliensis in the immune response of patients with paracoccidioidomycosis. The hyperimmunoglobulinemia E may be related to the T-cell defect that characterizes the severe forms of the disease.

Adolescent↗
PubMedDate not supplied

Immunological study of human strongyloidiasis. I. Analysis of IgE levels.

Total IgE serum levels were determined by the radioimmunosorbent method (RIST) in 18 patients with strongyloidiasis presenting mild or asymptomatic disease. Results showed elevated IgE serum levels in 7 out of 18 patients (38.8%), ranging from 150 to 3600 ng/ml with a mean concentration of 973 ng/ml.

Adult↗
PubMed1996

Radioimmunoprecipitation assay for glutamic acid decarboxylase antibodies evaluated clinically with sera from patients with insulin-dependent diabetes mellitus.

We evaluated a new, commercially developed radioimmunoprecipitation assay for measuring glutamic acid decarboxylase (GAD) antibodies by using recombinant human GAD65. The intra- and interassay CVs were 8.0% (n = 20) and 8.6% (n = 15), respectively. We found GAD antibodies in 74% (23 of 31; 95% confidence interval 55-88%), 70% (14 of 20; 46-88%), and 65% (28 of 43; 49-79%) of patients at, respectively, < or = 1 year, 1-2 years, and 2-4 years after the onset of insulin-dependent diabetes mellitus (IDDM) and in 30% (30 of 99; 21-40%) of patients with long-term diabetes (4-22 years). We also detected GAD antibodies in 8% (9 of 106; 4-16%) of patients with non-insulin-dependent diabetes mellitus (NIDDM). The frequency of GAD antibodies in the NIDDM group was markedly higher in the insulin-deficient patients [67% (6 of 9; 30-93%)], who initially were nonketotic and non-insulin-dependent for > or = 6 months but later became insulin dependent, than in the non-insulin-deficient patients [3% (3 of 97; 1-9%)]. This new commercial assay is easy to use and provides a specific and sensitive method for evaluating GAD antibodies in IDDM.

Adolescent↗
PubMed2006

Accuracy of B-type natriuretic peptide tests to exclude congestive heart failure: systematic review of test accuracy studies.

BACKGROUND: Congestive heart failure (CHF) is a major public health problem. The use of B-type natriuretic peptide (BNP) tests shows promising diagnostic accuracy. Herein, we summarize the evidence on the accuracy of BNP tests in the diagnosis of CHF and compare the performance of rapid enzyme-linked immunosorbent assay (ELISA) and standard radioimmunosorbent assay (RIA) tests. METHODS: We searched electronic databases and the reference lists of included studies, and we contacted experts. Data were extracted on the study population, the type of test used, and methods. Receiver operating characteristic (ROC) plots and summary ROC curves were produced and negative likelihood ratios pooled. Random-effect meta-analysis and metaregression were used to combine data and explore sources of between-study heterogeneity. RESULTS: Nineteen studies describing 22 patient populations (9 ELISA and 13 RIA) and 9093 patients were included. The diagnosis of CHF was verified by echocardiography, radionuclide scan, or echocardiography combined with clinical criteria. The pooled negative likelihood ratio overall from random-effect meta-analysis was 0.18 (95% confidence interval [CI], 0.13-0.23). It was lower for the ELISA test (0.12; 95% CI, 0.09-0.16) than for the RIA test (0.23; 95% CI, 0.16-0.32). For a pretest probability of 20%, which is typical for patients with suspected CHF in primary care, a negative result of the ELISA test would produce a posttest probability of 2.9%; a negative RIA test, a posttest probability of 5.4%. CONCLUSIONS: The use of BNP tests to rule out CHF in primary care settings could reduce demand for echocardiography. The advantages of rapid ELISA tests need to be balanced against their higher cost.

Biomarkers↗