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At least 325 records · Page 18Linked to original sources
PubMed2003

Effects of pH, temperature, and pre-pulsed electric field treatment on pulsed electric field and heat inactivation of Escherichia coli O157:H7.

This investigation was undertaken to study the inactivation of Escherichia coli O157:H7 by pulsed electric field (PEF) treatment and heat treatment after exposure to different stresses. E. coli O157:H7 cells exposed to different pHs (3.6, 5.2, and 7.0 for 6 h). different temperatures (4, 35, and 40 degrees C for 6 h), and different pre-PEF treatments (10, 15, and 20 kV/cm) were treated with PEFs (20, 25, and 30 kV/cm) or heat (60 degrees C for 3 min). The results of these experiments demonstrated that a pH of 3.6 and temperatures of 4 and 40 degrees C caused significant decreases in the inactivation of E. coli O157:H7 by PEF treatment and heat treatment (P < 0.05). Pre-PEF treatments, pHs of 5.2 and 7.0, and a temperature of 35 degrees C, on the other hand, did not result in any resistance of E. coli O157:H7 cells to inactivation by PEF treatment and heat treatment (P > 0.05).

Colony Count, Microbial↗
PubMed2003

[Study of elliptical centric view ordering technique with spectrally selected inversion recovery pulse (spec-IR pulse)].

Among several techniques for contrast-enhanced MRA, the elliptical centric view ordering method, originally developed at the Mayo Clinic, is a promising one. It has been difficult to apply a fat-suppression prepulse, mainly because the conventional fat suppression method requires a longer acquisition time during sampling of the low-frequency domain in k-space, and it causes severe image distortion due to the great change in longitudinal magnetization derived from fat tissue. We developed a novel method to append fat saturation to the elliptical centric view ordering technique, and assessed the feasibility of its use. Our method is to apply fat-saturation pulses only at selected sampling points when any gradient is applied in the slice-encoding direction. In this way, we achieved efficient suppression of fat-derived signal within a relatively short time, comparable to that of the conventional fat-saturation method, and succeeded in minimizing artifacts.

Artifacts↗
PubMed1992

Buccal pulse oximeter is more accurate than finger pulse oximeter in measuring oxygen saturation.

Although there have been several anecdotal reports of the use of buccal pulse oximeter monitoring (Spo2) when digital Spo2 monitoring cannot be used, there have been no objective evaluations of the accuracy of buccal Spo2 monitoring. The purpose of this study was to systematically compare buccal Spo2 monitoring to both digital Spo2 and arterial O2 saturation monitoring (Sao2) in both generally anesthetized patients in the operating room (n = 31) and critically ill patients in the intensive care unit (n = 23). Buccal Spo2 probes were prepared by taping a malleable metal bar securely over the back of a Nellcor Oxisensor D-25 probe and bending the metal bar and buccal probe firmly around the corner of the patient's mouth. All buccal and finger Spo2 and Sao2 measurements were made simultaneously during hemodynamic stability. We found that buccal Spo2 was higher than finger Spo2 and agreed more closely with Sao2 for both patient groups (98.1% +/- 2.6%, 96.8% +/- 3.5%, 98.5% +/- 2.5%, respectively [mean +/- SD]). The operating room patients had higher buccal and finger Spo2 and Sao2 (99.3% +/- 1.5%, 98.9% +/- 1.4%, 99.5% +/- 0.7%, respectively) than the intensive care unit patients (96.4% +/- 2.9%, 94.1% +/- 3.5%, 96.6% +/- 3.5%, respectively). Although buccal Spo2 monitoring has several disadvantages (i.e., the probe requires preparation, can be more difficult to place, may be less readily accepted in awake patients, and is often mechanically dislodged during airway maneuvers), we conclude that buccal Spo2 monitoring is a more than adequate oximetry alternative when digital Spo2 monitoring is not an option (digits are unavailable or available digits are mechanically interfered with).

Blood Gas Analysis↗