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Application of conventional (DC) and differential pulse polarography (DPP) to the quality control of benzothiadiazines in tablets. Simultaneous determination of hydrochlorothiazide and dihydralazine sulphate in mixtures with minimization of interaction by the use of a zero covariance in the calibration.

A previous study of the polarographic behaviour of 14 benzothiadiazines having diuretic properties enabled possible reaction mechanisms to be proposed and gave the opportunity to determine the best conditions for analytical application. The results obtained with benzthiazide as an example of the unsaturated compounds, bendrofluazide as the dihydro compound, and hydrochlorothiazide determined together with dihydralazine are reported. The electroactivity of these last two substances was made the object of a mathematical study for the correction of reciprocal interference.

Bendroflumethiazide↗

Polarography of Walker tumor submitted to radiotherapy.

A polarographic study of oxygen was done in 57 rats inoculated with Walker 256 tumor and platinum electrode implanted in muscle and in tumor. The goal of the research was the study of oxygen in tumor before and after irradiation. Tumor growth caused a decrease in tumoral oxygen. Oxygen was always lower in the tumor than in the muscle. Radiotherapy with 2000 rad (but not with 1000 rad) increased oxygen in the tumor.

Animals↗