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At least 325 records · Page 18Linked to original sources
PubMed1987

Tyrosine phosphorylation of common and specific sets of cellular proteins rapidly induced by insulin, insulin-like growth factor I, and epidermal growth factor in an intact cell.

KB cells respond to insulin and insulin-like growth factor I (IGF-I) in a closely similar way (induction of membrane ruffling, stimulation of pinocytosis, and amino acid transport) but respond to epidermal growth factors (EGF) in a similar but distinct way. In the KB cells, using phosphotyrosine-specific antibody we have found that: the receptors for insulin (beta subunit), IGF-I (beta subunit), and EGF undergo tyrosine phosphorylation as early as 10 s after addition of their respective ligands; a 185-kDa protein is rapidly (less than 10 s) tyrosine phosphorylated by insulin and IGF-I through their respective receptor kinases but not EGF; tyrosine phosphorylation of a 190-kDa glycoprotein is rapidly (less than 10 s) induced by EGF through EGF receptor kinase; and tyrosine phosphorylation of a 240-kDa protein is stimulated within 30 s by all three growth factors. These patterns of tyrosine phosphorylation could be causally related to biological responses induced by the three growth factors.

Carcinoma, Squamous Cell↗
PubMed1990

Subcellular distribution of a nitroxide spin-labeled netropsin-acridine hybrid in living KB cells: Electron Spin Resonance Study.

NETGA is an hybrid derivative which possesses an intercalating heterocyclic nucleus related to amsacrine and a minor groove binding squeletton related to netropsin. Cellular uptake of this drug has been studied by Electron Spin Resonance (ESR) spectroscopy using a spin-label derivative of NETGA (SL-NETGA). ESR determination of the kinetics of the drug repartition between the cytoplasm and nucleus showed that NETGA accumulated very rapidly and predominantly in the nucleus. Analysis of the anisotropic ESR spectra recorded in the nuclear compartment are in agreement with a strong binding of the drug to the DNA besides confirmed by a maximum delta Tm of 12 degrees C between the spin-label compound-DNA complex and the DNA alone.

Acridines↗