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At least 325 records · Page 18Linked to original sources
PubMed1999

Alpha-synuclein immunoisolation of glial inclusions from multiple system atrophy brain tissue reveals multiprotein components.

Immunohistochemical studies have shown that oligodendroglial inclusions in multiple system atrophy contain alpha-synuclein, a synaptic protein also found in Lewy bodies in Parkinson's disease. We have now used density gradient enrichment and an anti-alpha-synuclein immunomagnetic technique to isolate pure and morphologically intact oligodendroglial inclusions from brain white matter of patients dying with multiple system atrophy. Filamentous inclusion structures were obtained only from multiple system atrophy tissue, but not from normal brain tissues, or from multiple system atrophy tissue processed without anti-alpha-synuclein antibody. We confirmed the purity and morphology of isolated inclusions by electron microscopy. The inclusions comprised multiple protein bands after separation by polyacrylamide gel electrophoresis. Immunoblotting demonstrated that these proteins included alpha-synuclein, alphaB-crystallin, tubulins, ubiquitin, and prominent, possibly truncated alpha-synuclein species as high-molecular-weight aggregates. Our study provides the first biochemical evidence that oligodendroglial inclusion filaments consist of multiple protein components, suggesting that these inclusions may form as a result of multiprotein interactions with alpha-synuclein.

Aged↗
PubMed1999

[Formation of neuronal inclusions and neurodegeneration: with special reference to dentatorubral-pallidoluysian atrophy].

A new entity--the conformational disease--an otherwise diverse group of disorders, includes several currently incurable brain diseases such as Alzheimer's disease, prion encephalopathies and inherited polyglutamine diseases (PGDs) in which it is proposed that conformational changes in certain proteins and their subsequent aggregation represent the common pathogenic mechanism of neurodegeneration. In several PGDs including dentatorubral-pallidoluysian atrophy (DRPLA), neuronal intranuclear inclusions (NIIs) have been identified. Here I describe our recent studies on DRPLA. Formation of both intranuclear and intracytoplasmic inclusions by mutant DRPLA proteins with an expanded polyglutamine stretch was demonstrated in an in vitro experiment. Inclusion-containing non-brain cells were found to undergo apoptosis. In human DRPLA, similar intranuclear inclusions were detected in neurons in various brain regions. However, intracytoplasmic inclusions were found exclusively in neurons in the cerebeller dentate nucleus. The pontine nuclei neurons with NIIs, for instance, appeared to be small (atrophic), but healthy. The question of whether these NIIs actually kill brain cells in the human disease, remains unclear. Recently, NIIs were studied immunohistochemically using antibodies against transcription factors, and were found to contain TAFIIp130, Sp 1 and CREB-1. These results are of great interest when considering not only how the neurodegenerative mechanism progresses but also why the inclusions are located in nuclei in PGDs. Finally, Tsuji and colleagues recently established a transgenic mouse model of DRPLA. In these mice, NIIs, which were identical to those seen in the human disease, were confirmed in various brain regions. Further detailed studies of this model will contribute to a better understanding of the formation of neuronal inclusions and neurodegeneration in DRPLA.

Animals↗
PubMedDate not supplied

Ubiquitinated neuronal inclusions in the neostriatum in patients with amyotrophic lateral sclerosis with and without dementia--a study of 60 patients 31 to 87 years of age.

OBJECTIVE AND METHODS: Neuronal degeneration in amyotrophic lateral sclerosis (ALS) is associated with ubiquitinated cytoskeletal alterations in the motor neuron system. Ubiquitin-positive inclusions are also seen in the limbic system in ALS with dementia (ALS-D). Recently, similar inclusions were reported to occur in the neostriatum in a case of ALS-D. We, therefore, immunohistochemically examined the neostriatum from 60 patients with ALS and 60 control subjects. RESULTS: Two forms of ubiquitin-positive inclusions were found in the ALS neostriatum: rod-like inclusions in the large neurons and crescent-shaped inclusions in the small neurons. The former were found in 14 ALS and 18 control subjects, whereas the latter were specific to ALS; the crescent-shaped inclusions in small neurons were found in 27 ALS patients, and the immunohistochemical and ultrastructural features were identical to those of the extra-motor inclusions of ALS. Moreover, characteristic temporal lesions consistent with those seen in ALS-D were found in 8 patients, of whom 2 had shown dementia. CONCLUSION: The present findings strongly suggest that neostriatal small neurons are also involved in the disease process in ALS.

Adult↗
PubMed2001

Evidence that newly synthesized esterified cholesterol is deposited in existing cytoplasmic lipid inclusions.

Esterified cholesterol (EC) and triglyceride (TG) can be stored in cells as cytoplasmic inclusions. The physical state of the EC in these lipid droplets varies from liquid to liquid crystalline, depending on a number of factors, including the amount of TG co-deposited in the inclusion. The lipid in these droplets undergoes turnover via hydrolysis and resynthesis. We determined whether newly synthesized lipid is incorporated into existing cytoplasmic droplets, forms a discrete cytoplasmic droplet, or forms a small inclusion that fuses with an existing droplet. This was accomplished by monitoring the physical state of the lipid within the cytoplasmic inclusions following sequential deposition of TG and EC. Fu5AH cells were initially grown in media containing oleic acid to produce TG-rich, isotropic inclusions. The cells were then incubated with medium containing free cholesterol-phospholipid dispersions to promote synthesis and deposition of EC. To inhibit cytoplasmic TG hydrolysis, the lipase inhibitor, diethylumbelliferyl phosphate (UBP), was added at the time of cholesterol enrichment. The phase behavior of lipid droplets isolated from the lipid-rich cells was determined using polarizing light flow cytometry and microscopy. An anisotropic droplet population (EC-rich inclusions) was not detected, although there was an increase in cellular EC mass and no change in cellular TG mass. Therefore, under conditions where there is no turnover of cytoplasmic TG, newly synthesized EC is incorporated into existing TG inclusions.

Animals↗
PubMedDate not supplied

Relationship between age of flock seroconversion to hemorrhagic enteritis virus and appearance of adenoviral inclusions in the spleen and renal tubule epithelia of turkeys.

A retrospective study was conducted to evaluate the temporal relationship between flock seroconversion to hemorrhagic enteritis virus (HEV) and the appearance of adenoviral inclusions in the spleen and renal tubular epithelium. The study was conducted on samples of turkey poults submitted to the Fresno Branch of the California Veterinary Diagnostic Laboratory System during May to December 1988. The study included 78 submissions (four to eight poults per submission) of ages ranging from 6 to 15 weeks. Sera were tested for antibodies to HEV using the agar gel immunodiffusion test. Spleen and kidney samples were examined by light microscopy for the presence of inclusions in the mononuclear phagocytes of the spleen or in the renal tubular epithelium of the kidney. Logistic regression statistical analysis was used to evaluate the association between the age of the bird and the likelihood of the presence of inclusions in the spleen and kidney, as well as the likelihood of seroconversion to HEV. A significant association (P less than 0.05) was found between the presence of splenic inclusion bodies and the age of the bird. The probability of splenic inclusions was higher in younger birds (6 weeks of age), and decreased as the birds became older, approaching zero at 11 weeks of age. The kidney inclusions were significantly associated with age. The probability of detecting the inclusions increased with age, reached a maximum at 10 weeks, and then declined, approaching zero by 14 weeks. However, the probability of seroconversion to HEV increased significantly with age up to 10 weeks and then remained positive throughout the remainder of the study period.

Adenoviridae Infections↗
PubMed1992

The phase behavior of cholesteryl esters in intracellular inclusions.

Differential scanning calorimetry and polarizing light microscopy have been used to investigate kinetic and thermodynamic properties of the phase behavior of cholesteryl ester contained in Fu5AH rat hepatoma cells and J774 murine macrophages. These cultured cells store cholesteryl esters as cytoplasmic inclusions of approximately 1-micron diameter and thus are models of the foam cells characteristic of atherosclerotic plaque. Simple binary mixtures of cholesteryl palmitate and cholesteryl oleate, the predominant cholesteryl esters in cellular inclusions in both cell types serve as models to explain important aspects of the phase behavior of these inclusions. Although inclusions should exist as stable crystals at 37 degrees C under conditions of thermodynamic equilibrium, microscopic examination of cells indicates that inclusions exist as metastable liquid crystals at 37 degrees C for extended periods of time. Using an analytical model based on nucleation theory, we predict that the cholesteryl ester inclusions should be liquid-crystalline in the cytoplasm of living cells. This may not be true either for lysosomal cholesteryl ester or for extracellular cholesteryl ester present in advanced atherosclerotic plaque where fusion of droplets can enhance the possibility of crystallization. The enhanced metastability of the relatively fluid liquid-crystalline state in cellular inclusions should result in increased activity of the neutral cholesteryl ester hydrolase in living cells.

Animals↗
PubMed1975

Lead-induced inclusion bodies: results of ethylenediaminetetraacetic acid treatment.

Earlier studies have shown that lead-induced nuclear inclusion bodies are composed of a lead-protein complex. Twenty-four hours after treatment of a lead-poisoned rat with a single dose of ethylenediaminetetraacetic acid (EDTA), urinary excretion of lead is maximally increased and the lead content of the kidneys is significantly decreased. Inclusion bodies in renal cell nuclei at this time are found in various stages of dissolution and migration out of the nucleus. The outer fibrillary margin becomes loosened, and slight disruption occurs at the periphery of the inclusion. Some inclusion bodies appear to be moving through the nuclear membrane. There is an invagination of both inner and outer nuclear membranes. Numerous cytoplasmic vacuoles occur which contain fibrillar material resembling portions of intact nuclear inclusions. Kidney nuclei of rats 24 hours after three daily injections of EDTA have no inclusion bodies. Whether the nuclear-cytoplasmic exchange occurs by dilation of a nuclear pore or by another mode of separation of nuclear membranes cannot be determined. This experiment demonstrates that nuclear inclusion bodies formed in lead poisoning are disrupted and removed from the nuclei by the administration of EDTA. This change corresponds with peak urinary excretion of lead. The sharp increase in urinary lead following EDTA therapy must reflect, at least in part, chelation and excretion of sequestered lead bound to nuclear protein.

Animals↗
PubMedDate not supplied

Neuronal intranuclear hyaline inclusion disease: report of a case and review of the literature.

A case of neuronal intranuclear hyaline inclusion disease (NIHID) is described. The patient was a 26-year-old man who died of a progressive neurologic disorder, the onset of which occurred at the age of 11 years. Clinically, the disease presented as juvenile parkinsonism, and pathologically it was characterized by multiple-system degeneration in conjunction with the ubiquitous presence of intranuclear hyaline inclusions in neurons of the central and peripheral nervous system including the autonomic ganglia. Smaller and less eosinophilic intranuclear inclusions were also present in a small number of glial cells. The neuronal inclusions emitted a strong yellow-green autofluorescence under ultraviolet light and were composed of filaments 10-15 nm in diameter. The glial inclusions also consisted of similar filaments but their autofluorescence could not be determined with certainty because of their small size and background autofluorescence. A review of the literature revealed 19 similar autopsy cases up to 1987. Since the clinical presentation and distribution of neuronal loss as well as the characteristics of the inclusions showed some differences among the cases, some authors speculated that NIHID represented more than one variant of a multiple-system degenerative disease. However, about half of the reported cases had favorable sites of neurodegeneration, such as the pallidum, substantia nigra, motor nuclei of the brain stem, anterior horn cells, Clarke's column and spinal ganglion as well as similarities among the inclusions. Thus, there seems to be a discrete group among cases of NIHID.

Adult↗
PubMed1994

Intracytoplasmic granulofilamentous inclusion bodies in a meningioma. Immunohistochemical and ultrastructural studies.

A case of cerebellar tentorial meningioma, with abundant, large, eosinophilic intracytoplasmic inclusions, was studied immunohistochemically and ultrastructurally. These intracellular inclusions were periodic acid-Schiff negative and ultrastructurally appeared as a condensation or aggregation of numerous intracytoplasmic intermediate filaments. These inclusions were not related to microvillus-lined ductules, indicating that these inclusions were distinct from pseudopsammoma bodies. In addition, these inclusions were intensely immunopositive for vimentin, whereas staining for glial fibrillary acidic protein, epithelial membrane antigen, cytokeratin, neurofilament, desmin, actin, or type IV collagen was negative. To our knowledge, this is the second reported case of meningioma with such unusual inclusions, but the first one to demonstrate that the intracytoplasmic intermediate filaments and their related inclusions were mainly composed of vimentin.

Cytoplasm↗
PubMed1994

Twisted tubulofilaments of inclusion body myositis muscle resemble paired helical filaments of Alzheimer brain and contain hyperphosphorylated tau.

We immunostained muscle biopsies of 8 patients with sporadic inclusion body myositis (S-IBM), 7 patients with autosomal recessive hereditary inclusion body myopathy (H-IBM) (both diseases being characterized by similar muscle fiber vacuoles containing inclusions), and 11 normal and disease controls. We used the following well-characterized antibodies against tau protein: Tau-1, Alz-50, and anti-paired helical filament (PHF) antiserum. By light microscopy, in all S-IBM muscle biopsies virtually all vacuoles immunoreactive for ubiquitin and beta-amyloid protein also contained inclusions immunoreactive with Alz-50 and anti-PHF antiserum. With tau-1 antibody, strong immunoreactivity in the vacuoles was obtained only after dephosphorylation of muscle sections. By electronmicroscopy, all three antibodies immunodecorated exclusively cytoplasmic twisted tubulofilaments (TTFs). In H-IBM, virtually all ubiquitin and beta-amyloid-positive muscle fiber vacuoles contained inclusions immunoreactive with anti-PHF antiserum, but in only 40% of those fibers were the inclusions immunoreactive with Alz-50. In six H-IBM patients there were no tau-1 immunoreactive inclusions in any of their vacuolated muscle fibers; in one patient, 24% of the vacuolated fibers had tau-1 immunoreactivity. By demonstrating that hyperphosphorylated tau, which is characteristic of Alzheimer brain PHFs, is a component of S-IBM-muscle TTFs (which are also ultrastructurally similar to PHFs), our study: 1) provides the first demonstration of abnormally accumulated tau in nonneural tissue and 2) suggests that the cytopathogenesis in Alzheimer brain and S-IBM muscle may share some similar mechanisms. Whether the difference in tau immunoreactivity between S-IBM and most of the H-IBM patients reflects a difference in genetically determined transcriptional or posttranslational modifications of tau protein or other factors remains to be determined.

Adult↗
PubMed1993

Inclusion body formation and protein stability in sequence variants of interleukin-1 beta.

Inclusion body formation during recombinant protein expression in bacteria is of both fundamental interest and practical importance. To elucidate molecular mechanisms of this process, we are examining the in vitro folding and stability properties of a series of human interleukin-1 beta (IL-1 beta) sequence variants which exhibit widely differing tendencies to form inclusion bodies. Of 67 variants surveyed, nine, including wild type, were purified and their in vitro stability properties determined. One of these, a high inclusion body mutant, exhibited very low solubility in native buffer after purification and was not pursued further. For the other eight sequence variants, no strong correlations were observed between extent of inclusion body formation and either thermodynamic or thermal stability. In particular, a Lys97-->Val mutation produces substantially more IL-1 beta in inclusion bodies than the wild type (61 versus 8%) despite generating a protein more thermodynamically stable than wild type. Furthermore, the Lys97-->Val mutant forms substantial levels of inclusion bodies at 32 degrees C but requires incubation at temperatures greater than 48 degrees C for thermally induced aggregation in vitro. This and other data suggest that the tendency of at least some IL-1 beta variants to form inclusion bodies is most likely related to the stability or solubility of folding intermediates rather than native states. Implications of the structural locations of these mutations are also discussed.

Amino Acid Sequence↗
PubMed2002

Design and structural extension of a supramolecular inclusion-compound host made by the formation of dimers of isonicotinic acid and thiocyanato coordinating bridges.

A new host design for an inclusion compound with a preference for large planar aromatic guest molecules has been proposed. Our host design includes a rectangular cavity made using a long and a short building block based on the concept of supramolecular chemistry. The long building block facilitates the inclusion of large guests, and the short building block prevents the formation of an interpenetrated structure, which is often observed in frameworks with large void spaces. The long building block is made when dimers of 4-pyridinecarboxylic acid (isoH) form through hydrogen bonding between the two carboxylic acid moieties. This isoH dimer can link two transition metal centers using the N atoms at both ends to act as a long building block. For the short building block, the thiocyanato ion was used. This makes a bent bridge between two metal centers to form a 1D double-chain [M(SCN)2]infinity complex. From the self-assembly of isoH, SCN- and Ni2+, a 2D network of [Ni(SCN)2(isoH)2]infinity, in which the 1D [Ni(SCN)2]infinity complexes are linked by the isoH dimers, is built up. The rectangular cavity is formed as a mesh within the 2D network. The crystal of our inclusion compound has a layered structure of 2D networks, and a 1D channel-like cavity penetrating the layered 2D networks is formed where guests may be included. Moreover, our host design has the advantage of easy extension of the host structure. Replacement of isoH with another component and use of three components is possible for making the long building block. In the latter case, a linear spacer having two carboxy groups is inserted into the isoH dimer to form a long building block with a trimer structure. Based on our host design, a series of new inclusion compounds were synthesized. The crystal structures of three compounds were determined by single crystal X-ray diffraction. These were a biphenyl inclusion compound [Ni(SCN)2(isoH)2].1/2C12H10 (the basic case), a 9,10-dichloroanthracene inclusion compound [Ni(SCN)2(acrylH)2].1/2C14H8Cl2, where isoH is replaced with 3-(4-pyridinyl)-2-propenoic acid (acrylH), and a perylene inclusion compound [Ni(SCN)2(isoH)2(fumaricH2)].1/2C20H12, whose long building block is a trimer inserted with fumaric acid (fumaricH2) as a linear spacer.

Journal Article↗
PubMed1979

Intramitochondrial rod-like inclusions in human breast tumors.

Ultrastructural screening of human breast tumor biopsies has revealed, in 43% of the carcinomas, and in 16% of the benign lesions examined. the presence of rod-like inclusions in mitochondria of epithelial cells. The inclusions are cylindrical in cross section, with a diameter of about 50 nm, and show regular striations in longitudinal sections. They are probably derived from cristae. Mitochondria with inclusions are usually enlarged and are found most frequently in epithelial cells of invasive tumors and near zones of tumor necrosis. Similar although not identical inclusions have been observed by other investigators in the liver of women with hydatidiform mole or choriocarcinoma, of pregnant women, and of women taking oral contraceptives. Identical inclusions have been found in mitochondria of HeLa cells treated with anti-HeLa serum. Although no clear-cut correlations were found between the finding of inclusions and parameters such as age of patients, nuclear grade or lymphocytic infiltration of the breast tumors, it is conceivable that the presence of mitochondrial inclusions reflects the effect of hormonal or of immunological factors.

Adenofibroma↗
PubMed1994

Composition of Gomori-positive inclusions in astrocytes of the hypothalamic arcuate nucleus.

BACKGROUND: Astrocytes within the hypothalamic arcuate nucleus contain Gomori-positive inclusions that exhibit a nonenzymatic peroxidase activity. The source and composition of these Gomori-positive inclusions are currently unknown. Recent evidence, derived from cultured astrocytes, suggests that Gomori-positive inclusions may consist of autophagocytized accumulations of altered mitochondria and that the peroxidase activity is generated by iron or other metals which accumulate in these mitochondria. METHODS: The present study applies electron microscopy, energy dispersive X-ray microanalysis, and immunocytochemistry in conjunction with confocal microscopy to determine the structure and composition of Gomori-positive inclusions in vivo. RESULTS: The results indicate that Gomori-positive inclusions are heterogeneous structures often associated with microtubules and that they contain conspicuous mitochondrial components. Gomori-positive inclusions exhibit X-ray emission peaks for copper and, less often, chromium, either of which could account for the peroxidase activity. CONCLUSIONS: These results support the hypothesis that Gomori-positive inclusions are autophagosomes in which mitochondria are prominent.

Animals↗
PubMed2006

Site-selective formation of optically active inclusion complexes of alkoxo-subphthalocyanines with beta-cyclodextrin at the toluene/water interface.

Several subphthalocyanine derivatives that contain an alkoxo substituent as an axial ligand (RO-Subpc, R = 9-anthracenemethyl, benzyl, phenyl, 3,5-dimethylbenzyl, 3,5-dimethylphenyl, 4-methylbenzyl, and 4-methylphenyl) were synthesized. The formation of inclusion complexes of RO-Subpc with beta-CD in DMSO and at the toluene/water interface was investigated by UV/Vis absorption spectroscopy, induced circular dichroism (ICD), and nuclear magnetic resonance (NMR) measurements. Interfacial tension measurements suggested that beta-CD adsorbed as a monolayer at the toluene/water interface and probably orientated towards the toluene phase with its primary face. The 1:1 composition of beta-CD.RO-Subpc inclusion complexes was confirmed in DMSO and at the toluene/water interface for BzO-Subpc, PhO-Subpc, MeBzO-Subpc, and MePhO-Subpc. A 2:1 inclusion complex of AnO-Subpc formed in DMSO. The observed ICD spectra of beta-CDRO-Subpc inclusion complexes are discussed with respect to molecular modeling and the simulation based on Tinoco-Kirkwood theory. Interestingly, the ICD spectra of beta-CD.BzO-Subpc and beta-CD.MeBzO-Subpc inclusion complexes exhibited a negative sign in DMSO and a positive sign at the toluene/water interface. This reversal of the ICD sign strongly suggests a difference in the structure of the inclusion complexes: beta-CD at the interface formed the inclusion complex with its primary face, whereas the secondary face of beta-CD bound favorably to RO-Subpc in DMSO.

Carbocyanines↗
PubMed1992

Paramyxovirus-like nuclear inclusions identical to those of Paget's disease of bone detected in giant cells of primary oxalosis.

Nuclear inclusions, identical to those characteristic of Paget's disease of bone, were observed in giant cells in four of eight cases of primary oxalosis. The giant cells containing nuclear inclusions were directly involved in phagocytosis of large oxalate crystals in the context of typical foreign body granulomas in the bone marrow. Cytochemically, all of them exhibited strong tartrate-resistant acid phosphatase activity, and a proportion of them also tartrate-resistant acid ATPase. The inclusions consisted of typical arrays of filamentous material as described in Paget's disease, admixed with variable proportions of electron-dense material closely reminiscent of nucleolar pars fibrillaris and fibrillary centres. These data indicate: (a) the occurrence of Paget-like inclusions in a bone disease unrelated to Paget's disease, not causally related to viral infection, and resulting from an inborn metabolic derangement; and (b) the occurrence of Paget-like inclusions in foreign body giant cells as opposed to osteoclasts. We suggest that the occurrence of paramyxovirus-like nuclear inclusions in either osteoclasts or giant cells may represent an epiphenomenon of cell fusion and giant cell formation whenever appropriate stimuli act on latently infected precursor cells. Furthermore, our data suggest that nucleoli may represent the specific site of virus-like inclusion formation.

Acid Phosphatase↗
PubMed1988

An electron microscopic study of MPTP-induced inclusion bodies in an old monkey.

Eosinophilic inclusion bodies have recently been found in the nerve cells of the substantia nigra in aged primates with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced parkinsonism. This report describes the ultrastructure of the inclusion-bearing nerve cells in the substantia nigra from one of these animals. By electron microscopy the inclusions were less well demarcated and less conspicuous than those seen in hematoxylin-eosin stained paraffin sections. The bodies corresponded to portions of the nerve cell that were devoid of Nissl substance, but contained an increased number of randomly oriented, thick filamentous or tubular structures with some resemblance to neurotubules. The inclusion bodies were compared to Pick bodies: argentophilic inclusions in Pick's disease, a rare form of dementia, and to Lewy bodies: the inclusions characteristically present in Parkinson's disease. The MPTP-induced inclusions differed from both Pick bodies and Lewy bodies, but could represent immature Lewy bodies, similar to Lewy bodies in the cerebral cortex. The mechanism of formation of these structures, their relationship to Parkinson's disease and the reason why they appear to require the substrate of an aged nervous system, remain to be determined.

1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine↗
PubMed2005

Preparation and study on the inclusion complexes of two tanshinone compounds with beta-cyclodextrin.

Solid inclusion complexes of two tanshinones (Tans): tanshinone IIA (Tan IIA), tanshinone I (Tan I) with beta-cyclodextrin (beta-CD) were synthesized by coprecipitation method. The solid inclusion complexes were characterized by using several analytical techniques: (1)H NMR spectra, IR spectra and thermal analysis. Stoichiometry of the inclusion complexes of Tans with beta-CD or HP-beta-CD is 1:1 which was investigated in solution. The formation constants of the complexes were determined by UV spectrophotometry. For same kind of CD, the stability was in the order: Tan IIA > Tan I; for same guest, the stability was in the order: HP-beta-CD > beta-CD. The effect of temperature on the inclusion interaction was examined and the thermodynamic parameters of inclusion process, Delta G, Delta H, Delta S were determined as well. The experimental results indicate that the inclusion process was an exothermic and enthalpy-driven process accompanied with a negative entropic contribution. The inclusion interaction between CD and Tans satisfied the law of enthalpy-entropy compensation.

Abietanes↗