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Reversing cell polarity: evidence and hypothesis.

The long, rod-shaped cells of myxobacteria are polarized by their gliding engines. At the rear, A-engines push while pili pull the front end forward. An hypothesis is developed whereby both engines are partially dis-assembled, then re-assembled at the opposite pole when cells reverse their movement direction. Reversals are induced by an Mgl G-protein switch that controls engine polarity. The switch is driven by an oscillatory circuit of Frizzy proteins. In growing cells, the circuit gives rise to an occasional reversal that makes swarming possible. Then, as myxobacteria begin fruiting body development, a rising level of C-signal input drives the oscillator and changes the reversal pattern. Cells reverse regularly every eight minutes in traveling waves, the reversal period is then prolonged enabling cells to form streams that enlarge tiny random aggregates into fruiting bodies.

Bacterial Proteins↗

Heteromorphic incompatibility and efficiency of pollination in two distylous Pentanisia species (Rubiaceae).

BACKGROUND AND AIMS: Distyly has been hypothesized to promote cross-pollination by reducing intrafloral and geitonogamous self-pollination, and enhancing intermorph pollination. Distylous plants typically display both reciprocal herkogamy and a heteromorphic incompatibility system, which allows mating only between morphs. Distyly and its pollination consequences were examined in two Pentanisia species with long-tubed flowers which are pollinated almost exclusively by butterflies. METHODS: Anther and stigma heights were measured to quantify reciprocal herkogamy. The type of incompatibility system was determined by observing pollen tubes and seed production following controlled hand pollination. Pollen loads on pollinators and stigmas were also examined to assess the efficiency of intermorph pollen flow. KEY RESULTS: Pentanisia prunelloides and P. angustifolia exhibit reciprocal herkogamy and a host of ancillary dimorphisms, including pollen colour, exine sculpturing, stigmatic papilla shape and floral-tube pubescence. Controlled hand-pollinations revealed the presence of a strong heteromorphic incompatibility system in both species. The site of incompatibility differed between the morphs; intramorph pollen tubes were blocked in the style of the short-styled morph and on the stigmatic surface of the long-styled morph. Butterflies carried pollen from the short- and long-styled morphs primarily on their head and proboscis, respectively. Natural pollination resulted in a higher proportion of pollen transfer from long- to short-styled plants than vice versa. Nevertheless, fruit set did not differ between morphs. CONCLUSIONS: Both Pentanisia species are fully distylous. Reciprocal herkogamy results in pollen from the two morphs being carried on different locations on pollinators' bodies, which in turn promotes intermorph pollination. Intramorph pollination does not result in fertilization, because of an effective heteromorphic incompatibility system.

Flowers↗

Possible involvement of Pseudomonas fluorescens and Bacillaceae in structural modifications of Tuber borchii fruit bodies.

Previous studies on Tuber borchii fruit bodies in early maturation stages suggested a role of bacteria in sporocarp structural modifications. In order to verify this hypothesis, in the present study we investigated by means of microbial and ultrastructural approaches, the bacterial population of T. borchii sporocarps from intermediate maturation phases to advanced decomposition stages, paying particular attention to chitinolytic and cellulolytic bacteria and to their relationships with ascii and ascospores. We found that Pseudomonas fluorescens and spore-forming Bacillaceae, both able to degrade cellulose and chitin, are present inside the sporocarps in all maturation stages investigated. Moreover, rod-shaped bacteria seem able to erode ascus walls and colonize the interior of ascii containing mature spores. These results suggest a possible role of these bacteria in the process of ascus opening. Moreover, the presence of P. fluorescens and Bacillaceae on isolated mature spores after decontamination suggests an intimate association between these bacteria and the ascospores.

Ascomycota↗

The wake dynamics and flight forces of the fruit fly Drosophila melanogaster.

We have used flow visualizations and instantaneous force measurements of tethered fruit flies (Drosophila melanogaster) to study the dynamics of force generation during flight. During each complete stroke cycle, the flies generate one single vortex loop consisting of vorticity shed during the downstroke and ventral flip. This gross pattern of wake structure in Drosophila is similar to those described for hovering birds and some other insects. The wake structure differed from those previously described, however, in that the vortex filaments shed during ventral stroke reversal did not fuse to complete a circular ring, but rather attached temporarily to the body to complete an inverted heart-shaped vortex loop. The attached ventral filaments of the loop subsequently slide along the length of the body and eventually fuse at the tip of the abdomen. We found no evidence for the shedding of wing-tip vorticity during the upstroke, and argue that this is due to an extreme form of the Wagner effect acting at that time. The flow visualizations predicted that maximum flight forces would be generated during the downstroke and ventral reversal, with little or no force generated during the upstroke. The instantaneous force measurements using laser-interferometry verified the periodic nature of force generation. Within each stroke cycle, there was one plateau of high force generation followed by a period of low force, which roughly correlated with the upstroke and downstroke periods. However, the fluctuations in force lagged behind their expected occurrence within the wing-stroke cycle by approximately 1 ms or one-fifth of the complete stroke cycle. This temporal discrepancy exceeds the range of expected inaccuracies and artifacts in the measurements, and we tentatively discuss the potential retarding effects within the underlying fluid mechanics.

Animals↗

Paul E. Meehl's influence on experimental psychopathology: fruits of the nexus of schizotypy and schizophrenia, neurology, and methodology.

Paul E. Meehl made numerous contributions to clinical science and a hallmark of many of these contributions was their integrative nature. Meehl's positions on complex topics, especially one such as schizophrenia, were reflective of input from a variety of disciplines and levels of analysis. In this essay the authors focus on Meehl's uniquely rich contribution to our understanding of schizophrenia through his theoretical model of schizotypy, his abiding interest in exploring neurologically based indicators of schizophrenia liability and encouragement to others to pursue such indicators, and his passion for rigorous research methodology. Meehl's contributions in each of these areas continue to influence the direction and research strategies used in experimental psychopathology to illuminate the fundamental nature of schizophrenia. These contributions have also shaped inquiry into many other psychopathological entities.

History, 20th Century↗

Aging is a deprivation syndrome driven by a germ-soma conflict.

Evolution through natural selection can be described as driven by a perpetual conflict of individuals competing for limited resources. Recently, I postulated that the shortage of resources godfathered the evolutionary achievements of the differentiation-apoptosis programming [Rev. Neurosci. 12 (2001) 217]. Unicellular deprivation-induced differentiation into germ cell-like spores can be regarded as the archaic reproduction events which were fueled by the remains of the fratricided cells of the apoptotic fruiting body. Evidence has been accumulated suggesting that conserved through the ages as the evolutionary legacy of the germ-soma conflict, the somatic loss of immortality during the ontogenetic segregation of primordial germ cells recapitulates the archaic fate of the fruiting body. In this heritage, somatic death is a germ cell-triggered event and has been established as evolutionary-fixed default state following asymmetric reproduction in a world of finite resources. Aging, on the other hand, is the stress resistance-dependent phenotype of the somatic resilience that counteracts the germ cell-inflicted death pathway. Thus, aging is a survival response and, in contrast to current beliefs, is antagonistically linked to death that is not imposed by group selection but enforced upon the soma by the selfish genes of the "enemy within". Environmental conditions shape the trade-off solutions as compromise between the conflicting germ-soma interests. Mechanistically, the neuroendocrine system, particularly those components that control energy balance, reproduction and stress responses, orchestrate these events. The reproductive phase is a self-limited process that moulds onset and progress of senescence with germ cell-dependent factors, e.g. gonadal hormones. These degenerate the regulatory pacemakers of the pineal-hypothalamic-pituitary network and its peripheral, e.g. thymic, gonadal and adrenal targets thereby eroding the trophic milieu. The ensuing cellular metabolic stress engenders adaptive adjustments of the glucose-fatty acid cycle, responses that are adequate and thus fitness-boosting under fuel shortage (e.g. during caloric restriction) but become detrimental under fuel abundance. In a Janus-faced capacity, the cellular stress response apparatus expresses both tolerogenic and mutagenic features of the social and asocial deprivation responses [Rev. Neurosci. 12 (2001) 217]. Mediated by the derangement of the energy-Ca(2+)-redox homeostatic triangle, a mosaic of dedifferentiation/apoptosis and mutagenic responses actuates the gradual exhaustion of functional reserves and eventually results in a multitude of aging-related diseases. This scenario reconciles programmed and stochastic features of aging and resolves the major inconsistencies of current theories by linking ultimate and proximate causes of aging. Reproduction, differentiation, apoptosis, stress response and metabolism are merged into a coherent regulatory network that stages aging as a naturally selected, germ cell-triggered and reproductive phase-modulated deprivation response.

Aging↗

Adult phyllostomid (bat) enamel by scanning electron microscopy--with a note on dermopteran enamel.

This study assesses the enamel of five phyllostomids of differing feeding habits; only one example of the microchiropteran super-family Phyllostomoidae having previously been studied by SEM. A dermopteran was also examined to ascertain whether the enamel might reveal insectivore, chiropteran or primate characteristics. The five phyllostomids were found to display the additional crystallite discontinuity feature (minor boundary plane or seam) which is a major characteristic of all the bats we have so far examined - with the exception of two megachiropterans. The enamel of the four fruit and nectar feeders (Phyllostomus, Carollia, Glossophaga and Artibeus) is essentially similar and different to that of the blood feeder (Desmodus). The differentiating factor for the two groups is the poor degree of prism development in Desmodus; the prisms being restricted to the inner two thirds of the enamel over the cusps or sectorial ridge, and lacking in the greater part of the axial and the sulcular enamel. The poor prism development in the vampire bat raises interesting questions from both an ontological and a phylogenetic point of view. The dermopteran (Cynocephalus sp.) displays horse-shoe shaped prisms with associated minor boundary planes (seams); an appearance entirely similar to those microchiroptera we have examined. This finding could be advanced as evidence for a close phylogenetic relationship between the Dermoptera and Chiroptera as these features are not found to the same extent in insectivores or in primates; the other two orders to which dermopterans are assigned. The evolutionary significance of the seam feature is being studied further; it is very likely to be of importance in unravelling the history of mammalian enamel.

Animals↗

Three-dimensional localization of wild-type and myosin II mutant cells during morphogenesis of Dictyostelium.

Dictyostelium amoebae that lack myosin II (mhcA-) are unable to undergo morphogenesis. The cells aggregate slowly to form hemispherical mounds, but the mounds never extend a tip upward. Expression of developmentally regulated genes appears normal in the absence of morphogenesis. When mixed with an excess of wild-type cells, some mutant cells form differentiated spores; however, rescue is extremely inefficient (Knecht and Loomis, 1988). In order to assess how morphogenesis is normally accomplished and why mutants lacking myosin II cannot develop, a new method has been developed that allows individual amoebae to be localized and tracked at high resolution within the multicellular organism during development. Amoebae are labeled with a fluorescent dye at the beginning of starvation, mixed with an excess of unlabeled cells, and allowed to develop. The three-dimensional position of labeled cells in the multicellular organism is then determined using a laser scanning confocal microscope. Using this methodology, we have shown that labeled wild-type cells are randomly distributed throughout the organism and complete development normally. When labeled mhcA- mutant cells are mixed with a 20-fold excess of wild-type cells, they are non-randomly localized even at the earliest stages of development. Mutant cells in aggregation streams are found primarily at the edges of the streams and many cells never become part of the streams or are left behind as the wild-type cells complete aggregation. Those that are incorporated into the aggregate are found at the edge and base, the backs of slugs and the base of the fruiting bodies. A few mutant cells can be found in the sorus, where they presumably become spores. The segregation of mhcA- mutant cells to the outside of the wild-type aggregation streams argues that the mutant cells are unable to penetrate a mass of adhered, wild-type cells. We hypothesize that mutant cells lacking cortical integrity are unable to generate sufficient protrusive force to break the adhesion of wild-type cells to each other. This would make the mutants incapable of moving through a mass of cells (either mutant or wild type) or of changing shape when adhered to other cells. We propose that mutants lacking myosin II are unable to accomplish morphogenesis because they cannot move correctly in a three-dimensional mass of adhered cells.

Animals↗

Trading nutrition for education: nutritional status and the sale of snack foods in an eastern Kentucky school.

Overweight and poor nutrition of children in the United States are becoming issues of increasing concern for public health. Dietary patterns of U.S. children indicate they are consuming too few fruits and vegetables and too many foods high in fat and sugar. Contributing to this pattern of food consumption is snacking, which is reported to be on the increase among adults and children alike. One place where snacking is under increased scrutiny, and where it is being increasingly criticized, is in U.S. schools, where snack foods are often sold to supplement inadequate budgets. This article takes a biocultural approach to understanding the nutritional status of elementary school children in a rural community in eastern Kentucky. It pays particular attention to the ways in which the school's nutrition environment shapes overweight and nutritional status for many of the children, focusing on the sale of snack foods and the reasons behind the principal's decision to sell snack foods in the school.

Anthropology, Cultural↗

Detection of strawberry vein banding virus by polymerase chain reaction and dot blot hybridization.

Strawberry vein banding virus (SVBV) is one of seventeen members of the family Caulimoviridae. Natural infection with the virus is known in Fragaria species only. Infections caused by SVBV are often symptomless (1), but their significance increases in mixed infections with strawberry crinkle or strawberry latent C viruses (2,3). This virus has been originally found on strawberries in USA and firstly described by Frazier (4), but it is probably world-wide distributed by planting or breeding materials. SVBV has been observed on cultivated strawberries in North America, Australia, Brazil, Japan (5) and recently in Europe (6,7). The concentration of SVBV in infected plants is usually very low. Its detection by ELISA is impossible because of lack of specific antibodies. Evidence of the caulimovirus nature of SVBV has been confirmed by its circular dsDNA genome, shape and size of viral particles (8), presence of cytoplasmic inclusion bodies typical for caulimoviruses, and distant serological relationship with cauliflower mosaic virus (CaMV, 9). In this paper we present detection of SVBV by combination of two detection methods--polymerase chain reaction (PCR) and dot blot hybridization with a non-radioactive probe.

Caulimovirus↗

Homeobox genes and growth factors in regulation of craniofacial and tooth morphogenesis.

Homeobox genes encode a special group of transcription factors that regulate gene expression in the developing embryo. The so-called Hox-cluster genes were first discovered in the Drosophila (fruit fly). They specify the identity of body segments and their patterning along the anteroposterior axis. Other homeobox-containing genes appear to regulate patterning of the head and face. The function of the Msx-1 homeobox gene has been shown to be necessary for tooth development. In general, it is thought that special combinations of homeobox genes specify the patterning of individual structures. Bone morphogenetic proteins (BMPs) are growth factors belonging to the family of transforming growth factor-beta (TGF-beta). BMPs regulate bone and cartilage development, and individual BMPs have been shown to contribute to the shaping of various skeletal elements. BMPs regulate bone and dentin formation also postnatally, and they have therapeutic potential in reparative osteogenesis and odontogenesis. BMPs also act as inductive signals between tissue layers in the embryo, and they regulate the expression of several transcription factors, including homeobox-containing genes. BMP-4 has been identified as an epithelial inductive signal in tooth development. As it is produced by early dental epithelium and regulates tooth-specific gene expression in the dental mesenchyme, including Msx-1 expression, it may be an important signal for the initiation of tooth development.

Animals↗

The pollination of Bromelia antiacantha (Bromeliaceae) in southeastern Brazil: ornithophilous versus melittophilous features.

Bromelia antiacantha flowered from December to February and during this period the central leaves and bracts displayed a bright red colour. The inflorescence bears 150-350 flowers, with 10-35 flowers opening per day over 4-5 days. The flowers are dark magenta coloured with white margins, tubular-shaped with a wide opening, and their stigma is situated below the anthers. Anthesis began around 4:00 h and flowers lasted approximately 15 h. The highest nectar volume and sugar concentration occurred between 4:00-6:00 h; after this period, both decreased throughout the day. B. antiacantha is partially self-incompatible, non-autogamous, and therefore, pollinator dependent. The hummingbirds Thalurania glaucopis, Amazilia fimbriata, and Ramphodon naevius were its most frequent pollinators (55% of the visits), visiting flowers mainly in the afternoon. The scattered distribution of B. antiacantha promoted trap-lining behaviour of the hummingbirds, which favoured fruit set through xenogamy. Corolla colour, wide flower opening, sweet odour and concentrated nectar early in the day favoured bee visitation. Of the 38 % of bee visits, 96% were made by Bombus morio, mainly in the morning and their behaviour promoted self-pollination. The bee Trigona spinipes frequently acted as a pollen and nectar thief early in the morning, excluding most of the hummingbirds. Bromelia antiacantha has ornithophilous and melittophilous features, and despite being pollinated by two kinds of agents, its reproductive success depends on a given set of circumstances.

Animals↗

Floral morphometrics and the evolution of sexual dimorphism in Lycium (Solanaceae).

Plants of Lycium californicum, L. exsertum, and L. fremontii produce flowers that are either male-sterile (female) or hermaphroditic, and populations are morphologically gynodioecious. As is commonly found in gynodioecious species, flowers on female plants are smaller than those on hermaphrodites for a number of floral traits. Floral size dimorphism has often been hypothesized to be the result of either a reduction in female flower size that allows reallocation to greater fruit and seed production, or an increase in hermaphroditic flower size due to the increased importance of pollinator attraction and pollen export for hermaphroditic flowers. We provide a test of these two alternatives by measuring 11 floral characters in eight species of Lycium and using a phylogeny to reconstruct the floral size shifts associated with the evolution of gender dimorphism. Our analyses suggest that female flowers are reduced in size relative to the ancestral condition, whereas flowers on hermaphrodites have changed only slightly in size. Female and hermaphroditic flowers have also diverged both from one another and from ancestral cosexual species in several shape characteristics. We expected sexual dimorphism to be similar among the three dimorphic taxa, as gender dimorphism evolved only a single time in the ancestor of the American dimorphic lineage. While the floral sexual dimorphism is broadly similar among the three dimorphic species, there are some species-specific differences. For example, L. exsertum has the greatest floral size dimorphism, whereas L. fremontii had the greatest size-independent dimorphism in pistil characters. To determine the degree to which phylogenetic uncertainty affected reconstruction of ancestral character states, we performed a sensitivity analysis by reconstructing ancestral character states on alternative topologies. We argue that investigations such as this one, that examine floral evolution from an explicitly phylogenetic perspective, provide new insights into the study of the evolution of floral sexual dimorphism.

Biological Evolution↗

Taxonomic clarification of Cladosporium trichoides Emmons and its subsequent synonyms.

Cladosporium trichoides Emmons has been treated by some mycologists as a synonym of Cladosporium bantianum (Sacc.) Borelli and has been transferred to the genus Xylohypha (Fr.) Mason. In the present study, a herbarium specimen of C. bantianum (Torula bantiana Sacc.) Borelli, prepared by Saccardo, was compared with a herbarium specimen and a living type culture of C. triochoides by light and scanning electron microscopy (SEM) and was found to be dissimilar. Herbarium specimens and living cultures of Xylohypha nigrescens, the type species of the genus Xylohypha, were also compared with those of C. trichoides and other pathogenic Cladosporium species. Fundamental differences were found between X. nigrescens and Cladosporium species, in colony morphology, manner of sporulation and conidial morphology. All Cladosporium isolates produced olive-black colonies regardless of environmental conditions, bore brown pigment on the walls of the vegetative hyphae as well as on the walls of the fruiting structures and produced branched chains of conidia either from well differentiated or poorly differentiated conidiophores, or directly from the hyphae. By SEM, conidia showed strong to moderately protruded hila, and the basal contour of the conidia was always truncated. On germination, hyphal tubes were produced randomly from the surface of the conidia. In contrast, X. nigrescens produced white colonies with or without brown centres, depending on the culture medium, bore pigment on the conidial walls and on conidiogenous cells but not on the vegetative hyphae and produced infrequently branched conidial chains, usually from intercalary conidiogenous cells which were globose to hat-shaped. Conidial hila were nonprotruding but, instead, were deeply concave and pore-like. The basal contour of the conidia was round and germ tubes were produced only from the pore-like hila. These results indicate that C. triochoides Emmons is different from C. bantianum (Sacc.) Borelli and that the reclassification of C. trichoides into the genus Xylohypha was not warranted.

Animals↗

Attitudes of adolescents to their body build and the problem of juvenile obesity.

Prevalence of obesity, attitude to body weight and dimensions, eating habits, opinions on good nutrition and on the causes and prevention of obesity were studied in two groups of Israeli children, each comprising about 500 boys and girls, 13--14 years old. Mean relative weight was close to median weight for height, although 9 per cent of both boys and girls weighed more than 120 per cent of median weight. Weight was closely related to triceps skinfold thickness. Twenty-six per cent of the boys and 15 per cent of the girls rated themselves as thin, 61 per cent of children of both sexes as medium, and 13 per cent of the boys and 25 per cent of the girls as obese. Self-perception of body build was more closely related to weight than to skinfold thickness. Two-thirds of the boys and over one-half of the girls were satisfied with their weight, but dissatisfaction with size and shape was often expressed. Boys wished to have larger muscles and chest circumference and many girls, even when not obese, wanted to be smaller in size and shape. Most of the obese children wanted to lose weight. Of 499 children examined, 16 boys (7 per cent of the sample) and 58 (21 per cent) girls reported for dietary therapy. While most of these children were obese, by either subjective or objective criteria, an appreciable number of nonobese children were also dieting. The children's own perception of their body build seemed to be a strong motivation to diet for weight control. Most of the dieters did not receive any dietary advice from professional people. Over two-thirds of both boys and girls believed that daily consumption of milk, bread, fruits, eggs, cheese, meat, and tomatoes is desirable. More overweight than thin and normal-weight children indicated that, to prevent obesity, all kinds of food are permissible, but only in limited amounts. Most children believed in the fattening value of cakes, sweets, fried and fatty foods, potatoes, bread and nuts. Overweight children, particularly girls, reported eating less bread, cake and cream, adding less sugar to beverages, and eating sweets and ice cream less frequently than thin and normal-weight children. Overweight teen-agers appear to be more conscious of their food intake than under- and normal-weight children.

Adolescent↗

MPP+ analogs acting on mitochondria and inducing neuro-degeneration.

This review focuses on the mechanisms of action and the injurious effect of complex I inhibitors, of which 1-methyl-4-phenylpyridinium ion (MPP(+)) is a well studied example. These compounds can be divided into two groups, i.e. competitive inhibitors with respect to ubiquinone, such as piericidine A, and non-competitive inhibitors such as rotenone. Complex I inhibitors such as MPP(+) have been reported to induce anatomical, behavioral, and biochemical changes similar to those seen in Parkinson's disease, which is characterized by nigrostriatal dopaminergic neuro-degeneration. Spectroscopic analyses and structure-activity relationship studies have indicated that the V-shaped structure of the rotenone molecule is critical for binding to the rotenone binding site on complex I. Many isoquinoline derivatives, some of them endogenous, are also complex I inhibitors. Many lines of evidence show that complex I inhibitors elicit neuronal cell death. Recently, it was reported that chronic and systemic exposure to low-dose rotenone reproduces the features of Parkinson's disease. This work further focused attention on compounds acting on mitochondria, such as MPP(+). In Guadeloupe, the French West Indies, patients with atypical parkinsonism or progressive supranuclear palsy are frequently encountered. These diseases seem to be associated with ingestion of tropical herbal teas or tropical fruits of the Annonaceae family, which contain complex I inhibitors such as benzylisoquinoline derivatives and acetogenins. Complex I inhibitors may not simply result in reactive oxygen species generation or ATP exhaustion, but may influence complex downstream signal transduction processes. An understanding of these changes would throw light on the ways in which complex I inhibitors induce a wide range of abnormalities.

1-Methyl-4-phenylpyridinium↗

Profits for nonprofits: find a corporate partner.

Here's a familiar story. A nonprofit organization joins forces with a corporation in a caused-related marketing campaign. It seems like a win-win deal, but the nonprofit--and the media--find out several weeks into the campaign that the corporation's business practices are antithetical to the nonprofit's mission. The nonprofit's credibility is severely damaged. Is the moral of the story that nonprofits should steer clear of alliances with for-profit organizations? Not at all, Alan Andreasen says. Nonprofit managers can help their organizations avoid many of the risks and reap the rewards of cause-related marketing alliances by thinking of themselves not as charities but as partners in the marketing effort. More than ever, nonprofits need what many companies can offer: crucial new sources of revenue. But nonprofits offer corporate partners a great deal in return: the opportunity to enhance their image--and increase the bottom line--by supporting a worthy cause. Consider the fruitful partnership between American Express and Share Our Strength, a hunger-relief organization. Through the Charge Against Hunger program, now in its fourth year, American Express has helped contribute more than +16 million to SOS. In return, American Express has seen an increase in transactions with the card and in the number of merchants carrying the card. How can nonprofit managers build a successful partnership? They can assess their organization to see how it can add value to a corporate partner. They can identify those companies that stand to gain the most from a cause-related marketing alliance. And they can take an active role in shaping the partnership and monitoring its progress.

Breast Diseases↗

Three-dimensional analysis of mandibular morphology in Otolemur.

Euclidean distance matrix analysis (EDMA) of three-dimensional data is used here to examine mandibular morphology between two species of galagos. Otolemur crassicaudatus consumes primarily exudates, while O. garnettii is more frugivorous. Acquisition of exudates involves either gouging or scraping tree bark, and may involve different forces at the mandible than incising fruits. Previous studies of mandibular morphology in exudate-feeding primates produced mixed results, some suggesting that morphological specializations reflect adaptations for greater force at the anterior dentition, while others suggest specializations for producing a large gape. This study addresses these controversies by testing predictions associated with O. crassicaudatus generating greater force at the anterior dentition or producing a larger gape relative to O. garnettii. In addition, this study tests predictions associated with specializations of the anterior dentition in O. crassicaudatus as related to exudate-feeding. Crania and mandibles from 28 O. crassicaudatus and 17 O. garnettii were digitized in three dimensions, using 18 landmarks that summarize the shape of the jaw. Two-dimensional measurements were taken to assess incisor robusticity. All three-dimensional data were analyzed using EDMA, and bootstrap tests were executed to identify specific interlandmark differences that were driving any significant (P < 0.05) overall shape differences. Two-dimensional data were analyzed using Student's t-test for independent measures. Results revealed that there was a significant shape difference in mandibles between species, and that mandibles of O. crassicaudatus showed higher condyles, longer mandibles, decreased incisor procumbency, and greater incisor robusticity relative to O. garnettii. It is suggested that the results of the present study reflect adaptations for scraping in O. crassicaudatus rather than gouging.

Animals↗