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PubMed1998

[Actinomyces infection in a compound odontoma].

INTRODUCTION: Compound odontoma is an abnormal development of dental tissue which is characterized by regular and ordinate structures that resemble a normal tooth. Actinomyces is an abnormal inhabitant of the oral cavity which is able to become pathological whenever the specific or non specific defense mechanisms of an individual may fail. MATERIALS AND METHODS: Described is a case of 60 years old male with no deficit of his immune system confirmed by various analysis. There is one year history of an intermittent pain to the left half of his mandible. An x-ray suggests an odontoma. The gengiva, near the lesion, appears to be ulcerated; two fistulous tracts with the oral mucosa and one with the skin area are seen. After decalcification the tissue obtained from the lesion has been fixed in formalin and included in paraffin. The slides were stained with Haematoxilin-Eosin, PAS and Grocott's silver method. RESULTS: The lesion consists of well differentiated enamel and dentin including a fragment of the pulp featuring the typical aspects of compound odontoma. In addition the pulp shows an intense infiltration of granulocytes, plasma cells and numerous capillaries; around the pulp an extensive area of necrosis is present, in which there are eosinophilic aggregates of amorphic and granular matrix suggestive of an infection by actinomyces. This was confirmed with PAS and by an intense urgyrophilia. CONCLUSION: Described is a case of compound odontoma complicated by an actinomycosis infection in a patient with a well functioning immune system which appears to be the first case reported in the literature, to the best of our knowledge.

Actinomycosis↗
PubMed2000

Immunohistochemistry and microwave decalcification of human temporal bones.

Processing of human temporal bones is a long, expensive process and the resulting celloidin sections are difficult to use for immunohistochemistry. We tested the ability of immunohistochemical assays to work in human temporal bones that were decalcified using a microwave oven. Tissue was trimmed to an approximate cube (1.5-2 cm/side) containing only the cochlea and immersed in fresh EDTA with paraformaldehyde every 6 h. This sized block required 190-400 h to decalcify. The decalcified tissue was embedded in paraffin and sectioned. Sections were immunoassayed with anti-cytochrome c oxidase, anti-neurofilament or anti-peripherin. All three antibodies labeled the appropriate structures. This procedure may stimulate advancement in the understanding of human inner ear pathology.

Calcium↗
PubMed2003

Eustachian tube goblet cell density during and after acute otitis media caused by Streptococcus pneumoniae: a morphometric analysis.

BACKGROUND: Prior investigations have shown that the number of mucus-producing goblet cells in the middle ear mucosa is highly increased during and up to at least 6 months after experimental acute otitis media. This may, in conjunction with deteriorated eustachian tube function, predispose to subsequent development of secretory otitis media. One reason for the deteriorated tubal function after acute otitis media has been suggested to be an excessive accumulation of mucus secretions, blocking the tube and thereby clearance of the middle ear. This investigation determines the density of the mucus-producing goblet cells in the eustachian tube during and up to 6 months after experimental acute otitis media. METHODS: Middle ear inoculation of Streptococcus pneumoniae in 25 rats. Groups of five animals, killed on Days 4, 8, 16, 90, and 180. Dissection and decalcification of the eustachian tube, followed by paraffin embedding and serial transverse sectioning, periodic acid-Schiff/Alcian blue staining and morphometric determination of the goblet cell density in every 20th section, using a light microscope. RESULTS: The goblet cell density was increased on Day 8 and later in the tympanic orificium, in addition to the tympanic and midportion third of the tube. Increased goblet cell density was seen in the pharyngeal third on Days 8 and 16, whereas no changes were registered in the pharyngeal orificium. Pathologic intraepithelial glands formed after the infection and goblet cells were found in mucosal areas normally devoid of these. CONCLUSIONS: The eustachian tube goblet cell density is increased during and up to 6 months after acute otitis media. Indicated excessive secretion of mucus by more goblet cells may contribute to the deteriorated eustachian tube function found after acute otitis media and thus predispose, sustain, or aggravate middle ear disease.

Acute Disease↗
PubMed1996

Percutaneous transcatheter therapy of aorto-ostial stenoses.

The treatment of native coronary and saphenous vein graft aorto-ostial stenoses with balloon angioplasty (PTCA) has been associated with lower procedural success rates, more frequent in-hospital complications and a greater likelihood of late restenosis when compared with PTCA of non-ostial stenoses. The advent of ablative technologies and intracoronary stents have significantly altered both early and late outcomes of percutaneous intervention for aorto-ostial disease. The optimal approach to this complex lesion subset often involves decalcification or tissue ablation followed by stent deployment. Improvements in currently available stent technology including enhanced radial force and visibility, reduced thrombogenicity, and the availability of shorter stent lengths, may also facilitate stent deployment and further improve outcomes following percutaneous transcatheter therapy for aorto-ostial stenoses.

Angioplasty, Balloon, Coronary↗
PubMed1986

Sudden cardiac death and acute pathology of coronary arteries.

The pathology of sudden cardiac death still is a matter of controversy, particularly with respect to the state of the coronary arteries. A recent study has shown a high incidence of acute lesions and suggests a causal relationship. The present study has been designed to verify whether or not acute coronary arterial lesions occur frequently in patients with sudden cardiac death. Sixteen hearts were available. In each instance the patient had died within 6 hours from the onset of myocardial ischaemia. The coronary arterial system was extensively studied by post-mortem coronary angiography, decalcification, serial blocking and histological sectioning. Acute coronary arterial lesions, defined as plaque fissure, 'isolated' plaque haemorrhage and 'isolated thrombosis', were found in 14 of the 16 hearts (87.5%). The total number of acute lesions in the 14 hearts was 28. In 9 hearts plaque fissures were present, in 7 'isolated' plaque haemorrhages and in 5 an 'isolated thrombus'. Half of all acute lesions had occurred in an atherosclerotic plaque of pre-existing 50-75% luminal narrowing. The present study endorses the concept that acute coronary arterial lesions occur in a high proportion of patients with sudden cardiac death, plaque fissures with intramural haemorrhage and intraluminal thrombosis being the most common abnormality. It is tempting to attribute causal significance to such lesions in patients with sudden cardiac death.

Acute Disease↗
PubMed1994

Microwave histoprocessing of bone marrow trephine biopsies.

In recent years, the microwave oven has been increasingly used in the pathology laboratory for processing of tissue for diagnostic purposes with a remarkable reduction in processing time and also reports of excellent morphology and immunohistochemistry. We evaluated some of these processes on post mortem bone marrow trephine biopsies and describe a novel way of processing these biopsies in the microwave oven.

Biopsy↗
PubMed1975

Arteriosclerosis of coronary arteries in sudden, unexpected deaths.

The authors graphically studied the topographic pattern and severity of coronary arteriosclerosis in arteriosclerotic heart disease (ASHD) sudden deaths using an improved technique involving in toto removal and decalcification of the main coronary tree. The study involved 171 cases of ASHD sudden death and 154 deaths from other causes. White males were the most severely affected group. The majority of the ASHD deaths had three or four major coronary vessels showing greater than 75% luminal stenosis; single-artery disease was a rare occurence. The intra- and interarterial pattern of coronary stenosis was equally severe and diffuse, with the exception of the arteries to SA and AV nodes. No small intramyocardial blood vessel disease was evident. Severe chronic coronary stenosis was associated with a high incidence of old myocardial infarction. The anatomical and pathological pattern of coronary stenosis in ASHD deaths appears to have ominous therapeutic implications.

Adult↗
PubMed1993

Immunohistochemical detection of interstitial collagens in bone and cartilage tissue remnants in an infant Peruvian mummy.

We investigated the immunohistochemical presence of various collagen types in bone and cartilage tissue from an infant Peruvian mummy dating between 500 and 1000 A.D. which had been excavated at the necropolis of Las Trancas in the Nazca region in Peru. Following careful rehydration and decalcification of the tissue, the mummy tissue showed morphologically good preservation of the matrix, which could be shown to be composed of various collagen types in a typical pattern. Bone consisted of a collagen I matrix with a small rim of collagen III and V at the endosteal lining and a pericellular collagen V staining around osteocytic holes. In the hypertrophic cartilage of the epiphyseal growth plate, a typical pattern of collagen types II and X could be found. These observations provide evidence that in well-preserved mummy tissue the antigenic determinants of major matrix components are still adequately preserved for an immunohistochemical analysis. This technique may thus be a very helpful tool for the analysis of pathologic processes of historic bone tissue. It may also allow in certain circumstances a distinction between pseudopathologic tissue destruction and pathologic tissue alteration.

Bone and Bones↗
PubMed2004

Physiological, pharmacokinetic, and pharmacodynamic changes in space.

Medications have been taken since the first Mercury flight in 1967 and, since then, have been used for several indications such as space motion sickness, sleeplessness, headache, nausea, vomiting, back pain, and congestion. As the duration of space missions get longer, it is even more likely that astronauts will encounter some of the acute illnesses that are frequently seen on Earth. Microgravity environment induces several physiological changes in the human body. These changes include cardiovascular degeneration, bone decalcification, decreased plasma volume, blood flow, lymphocyte and eosinophil levels, altered hormonal and electrolyte levels, muscle atrophy, decreased blood cell mass, increased immunoglobulin A and M levels, and a decrease in the amount of microsomal P-450 and the activity of some of its dependent enzymes. These changes may be expected to have severe implications on the pharmacokinetic and pharmacodynamic properties of drug substances.

Aerospace Medicine↗
PubMed1978

[Methods of histological treatment of the labyrinth].

The otic labyrinth presents one of the most difficult objects for histological investigations as it is situated in the thick layer of the temporal bone and has a very complex structure. At the same time, a method for treating this object is not given in general hand-books on pathological techniques. The methods for histological treatment of the otic labyrinth elaborated by Wittmaack and later on modified are presented in the article. Methods for fixation, vital fixation including, decalcination, dehydratation and saturation of the objects prepared for histological investigation are described in details. Methods for the temporal bone orientation in order to obtain sections of the internal otic structures in the most advantageous plane depending on the purpose and aim of the investigation are recommended.

Animals↗
PubMed2002

[Morphological diagnosis of spinal diseases. Current technical possibilities and challenges for the histological preparation of transpedicular biopsies].

The spine is the central component for the mobility of the human body. Both locally limited and diffuse pathologies of the spine are a challenge for the treating physician due to the difficult anatomy. The biopsy of a pathologically altered vertebral body is a fast and reliable basis for further therapy but until now this has not regularly been made use of as a diagnostic standard for spinal diseases, since the tissue gained was often microfractured because of the difficult anatomical position. Our own experience with transpedicular vertebral biopsies of 70 patients with different diseases is reported because of the considerable improvement in the biopsy technique and the methodical possibilities for processing the bony tissue. Methods which have proven particularly valuable are contact radiographs, embedding in plastic, careful decalcifying with EDTA and immunohistological procedures. In this way a definite diagnosis can be made in 97% of the cases. A close cooperation with the clinician carrying out the biopsy and a greater use of methods other than just fast decalcification is recommended.

Biopsy↗
PubMed1976

Pathology of acute myocardial infarction with particular reference to occlusive coronary thrombi.

Analysis of the pathological findings in 500 cases of fatal acute myocardial infarction showed that in 469 this was localized to one transmural area of the left ventricle; in 31 there was diffuse subendocardial necrosis. In the former occlusive coronary thrombus was found in the related artery in 95 per cent of cases. Variation in the percentage of occlusions found was noted between different prosectors and when coronary artery calcification was present. Only 4 of the 31 patients with subendocardial necrosis had recent occlusion; triple vessel disease was common in this group suggesting general failure of coronary perfusion. It is essential in necropsy studies of the relation of coronary thrombosis to myocardial infarction to be sure that muscle necrosis is present, to distinguish the two forms of myocardial necrosis, and to employ a meticulous dissection technique with decalcification of the arteries when necessary.

Acute Disease↗
PubMed1998

Prenatal development of the normal human vertebral corpora in different segments of the spine.

STUDY DESIGN: Vertebral columns from 13 normal human fetuses (10-24 weeks of gestation) that had aborted spontaneously were investigated as part of the legal autopsy procedure. The investigation included spinal cord analysis. OBJECTIVES: To analyze the formation of the normal human vertebral corpora along the spine, including the early location and disappearance of the notochord. SUMMARY OF BACKGROUND DATA: Reference material on the development of the normal human vertebral corpora is needed for interpretation of published observations on prenatal malformations in the spine, which include observations of various types of malformation (anencephaly, spina bifida) and various genotypes (trisomy 18, 21 and 13, as well as triploidy). METHODS: The vertebral columns were studied by using radiography (Faxitron X-ray apparatus, Faxitron Model 43,855, Hewlett Packard) in lateral, frontal, and axial views and histology (decalcification, followed by toluidine blue and alcian blue staining) in and axial view. Immunohistochemical marking with Keratin Wide Spectrum also was done. RESULTS: Notochordal tissue (positive on marking with Keratin Wide Spectrum [DAKO, Denmark]) was located anterior to the cartilaginous body center in the youngest fetuses. The process of disintegration of the notochord and the morphology of the osseous vertebral corpora in the lumbosacral, thoracic, and cervical segments are described. Marked differences appeared in axial views, which were verified on horizontal histologic sections. Also, the increase in size was different in the different segments, being most pronounced in the thoracic and upper lumbar bodies. The lower thoracic bodies were the first to ossify. The morphologic changes observed by radiography were verified histologically. CONCLUSIONS: In this study, normal prenatal standards were established for the early development of the vertebral column. These standards can be used in the future--for evaluation of pathologic deviations in the human vertebral column in the second trimester.

Embryonic and Fetal Development↗
PubMed2000

[Demonstration of the calcified osseous component in decalcified bone marrow biopsies. Study of hematological cases].

In order to investigate the possibility of the evidentiation of the mineralized component of decalcified bone a series of Jamshidi-type consecutive bone marrow biopsies for various hematological disorders were pre-stained with von Kossa modified procedure for calcium. Methodologically the controls showed reliable silver staining as far as localization (osteo-medullary interface and mineralization front) and preservation (after decalcification) were concerned. The results were: 1) a good morphology of bone marrow tissue also concerning immunohistochemical stainings; 2) a better overview of the osseous components and related artifacts as induced by biopsy, processing and sampling; 3) precise identification of the osteoid seams and remarkably of the relative angle of sectioning for an appropriate measurement; 4) better evidentiation of the remodelling osteoblastic-osteoclastic units; 5) visualization of the osteocytic lacunae and canaliculi and the mineralized matrix to some extent depending on their effective permeability. Summarizing the osteologic features were: normality or minimal abnormality of difficult interpretation; classical osteometabolic alterations; lesions specifically due to hematological disorders; various combination of these findings. Theoretical and practical aspects are discussed. In conclusion this methodological variant in comparison with the usual paraffin procedure clearly gives more information concerning osteometabolic evaluation in routine hematological biopsies; offers a vicarious or complementary approach to osteometabolic diseases; represents a conceptual stimulus to interpret diagnostically and prognostically the osseous pathology as determined by routinely encountered hematological disorders.

Artifacts↗
PubMed1995

MIC2 detection in tumors of bone and adjacent soft tissues.

The diagnosis of Ewing's sarcoma has been based classically in large part on the exclusion of other similar small round-cell tumors by light microscopic and histochemical criteria. This study was undertaken to explore the use of a recently developed immunohistochemical stain directed against the glycoprotein p30/32MIC2 antigen (the gene product of MIC2), as a diagnostic tool and as a probe for the examination of potential interrelationships among the putative members of the family of peripheral primitive neuroectodermal tumors. Fifty-six small round-cell tumors of bone were selected for study from the files of the Armed Forces Institute of Pathology and Rhode Island Hospital; all tissues had been formalin fixed and paraffin embedded. Nine of 10 Ewing's sarcomas were MIC2 positive, as were 2 of 3 atypical Ewing's sarcomas (small round-cell tumors that diverged from the classic pattern of Ewing's sarcoma by exhibiting a greater degree of cytologic atypia and pleomorphism), and 7 of 8 Askin tumors of the thoracopulmonary region. Ten of 11 mesenchymal chondrosarcomas, 1 primitive neuroectodermal tumor of bone, 10 small cell osteosarcomas, 10 malignant lymphomas, and 3 sarcomas of bone (not additionally subclassified) were negative. The finding of MIC2 positivity in the majority of Ewing's sarcomas and Askin tumors provides additional support for earlier proposals (based on a shared cytogenetic abnormality, among other criteria) that these lesions be considered members of the same family, the peripheral primitive neuroectodermal tumors. The present study, drawing on archival and current case material (including decalcified and undecalcified specimens), indicates that neither the specimen age nor the application of any of a variety of decalcification solutions appears to adversely influence MIC2 staining of paraffin-embedded tissues. This suggests that this antibody has use in retrospective and prospective studies. The rare occurrence of false negative (in the case of Ewing's sarcoma) and positive results in tumors other than peripheral primitive neuroectodermal tumors (as in 1 of the mesenchymal chondrosarcomas) suggests that MIC2 staining should not be relied on as the sole criterion for identification or exclusion of Ewing's sarcomas and related tumors.

12E7 Antigen↗
PubMed2002

A histologic study of fractured human vertebral bodies.

Twenty-seven fractured human vertebral bodies and 24 unfractured human vertebrae from adjacent levels were studied postmortem using histologic and high-resolution radiographic techniques. The findings were compared with those in the vertebral bodies of individuals without spinal fracture. Forty-six human thoracolumbar spines were obtained from individuals at autopsy. Standard radiographs were made of all specimens. Twelve of the 46 individuals had a total of 27 fractured vertebral bodies by plain radiographic criteria. Attention was focused on these fractured vertebrae as well as on 24 unfractured vertebral bodies that were harvested from a level immediately adjacent to the fractured vertebral bodies. Twelve vertebral bodies from four individuals with no evidence of fracture or inflammatory spondyloarthropathy were also studied for comparison. The vertebral bodies were graded by their mineral density as measured by dual-energy x-ray absorptiometry and sectioned into 3-mm sagittal cuts. High-resolution contact radiographs were prepared for each section prior to decalcification and tissue sectioning on a large format microtome. Mid-and parasagittal tissue sections of each vertebra were prepared for standard hematoxylin and eosin stains. A total of 126 sections were studied. The histologic characteristics of the fractured vertebrae (n = 27) were compared with those of adjacent unfractured levels (n = 24) and with vertebrae from individuals without fracture (n = 12). Vertebral bodies with fractures secondary to osteoporosis were consistently characterized histologically by focal areas of endochondral new bone formation adjacent to avascular necrotic bone and unreactive marrow. Such ongoing new bone and new vessel formation adjacent to nonhealing areas were also documented in radiographically unfractured vertebral bodies from individuals with osteoporotic fractures at adjacent levels. No areas of endochondral new bone formation or areas of focal necrosis were found in vertebral bodies from individuals without radiographic evidence of osteoporosis. A vascular necrosis of the vertebral body is a common histologic finding in individuals with osteoporosis. Indeed, our histologic observations suggest subclinical fractures and microfractures of the vertebral body may be the underlying pathologic process leading to avascular necrosis in individuals with osteoporosis. Microtrabecular fractures and endplate fractures were commonly seen in osteoporotic vertebral bodies, often in vertebrae that appeared to be uninvolved on specimen radiographs.

Adult↗
PubMed1992

Immunohistochemical localization of collagenous components in healthy periodontal tissues of the rat and marmoset (Callithrix jacchus). I. Distribution of collagen types I and III.

The distribution of collagen types I and III was demonstrated in healthy periodontal tissues of the rat and marmoset using immunofluorescent localization after decalcification of the maxillae and mandiblae in 0.2 N HCl. An intense fluorescence in the alveolar bone and cementum matrix, as well as in the soft periodontal tissue, was demonstrated with anti-collagen type I antibodies. In the gingival connective tissue and in the periodontal ligament thick fibers of collagen type I could be observed. The fluorescent reaction in the rat periodontal ligament was not strong in comparison to the marmoset periodontal ligament. Sharpey's fibers, inserting into the cementum and alveolar bone, were also stained. On the other hand, collagen type III could not be demonstrated in the hard periodontal tissues, but could be in the bone marrow stroma and the incremental lines as well as around the Sharpey's fibers of the cementum, in accordance to previous studies. In the gingival connective tissue a strong staining was evident, especially near the basement membrane. The periodontal ligament showed an intense fluorescence that was, in some areas, continuous with Sharpey's fibers inserting into the cementum. The distribution of collagen types I and III was demonstrated with immunohistochemical techniques in the rat and marmoset periodontium. These results provide necessary information on healthy tissues that will be required for future studies on the effects of pathological, reparative and regenerative processes.

Alveolar Process↗
PubMed1991

Immunohistochemical localization of collagenous components in healthy periodontal tissues of the rat and marmoset (Callithrix jacchus). II. Distribution of collagen types IV, V and VI.

The immunohistochemical distribution of collagen types IV, V and VI has been demonstrated in healthy periodontal tissues of rats and marmosets following decalcification of the maxillae and mandibulae in 0.2 N HCl. An intense fluorescence with anti-collagen type IV antibodies was demonstrated in the basement membranes of the epithelium and of the blood vessels and nerves. In the alveolar bone stroma and in the periodontal ligament (PL) collagen type IV was present only in the basal membranes of the blood vessels and nerves. In comparison, collagen type V was observed in a fibrillar pattern in the gingival connective tissue, as well as the PL. In the PL, type V collagenous fibers demonstrated a parallel distribution with stronger fluorescence near the cementum surface. Collagen type VI could be demonstrated in fine fibers present in the gingival connective tissue and the PL. Blood vessels and nerves were not stained in the marmoset, but were in the rat, where a localization of collagen type VI was demonstrated in these areas. Alveolar bone and cementum, as well as the Sharpey's fibers embedded in these tissues, were not stained with antibodies against collagen type V and type VI, but a pericellular localization of these collagenous components could be observed. Collectively, these results provide basic information on the relative distribution of different collagen types in normal tissues of rats and marmosets that will be required for future studies on the effects of pathological, reparative and regenerative processes.

Animals↗