Morphological changes of pigeon crop-sac mucosa and pituitary lactotrophs after systemic and intraventricular administration of neuroleptic drugs.
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Animals↗
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Self-excision of the antibiotic resistance gene nptII using a heat inducible Cre-loxP system from transgenic potato.
Resistance to antibiotics mediated by selectable marker genes remains a powerful selection tool for transgenic event production. However, regulatory agencies and consumer concerns favor these to be eliminated from food crops. Several excision systems exist but none have been optimized or shown to be functional for clonally propagated crops. The excision of the nptII gene conferring resistance to kanamycin has been achieved here using a gene construct based on a heat-inducible cre gene producing a recombinase that eliminates cre and nptII genes flanked by two loxP sites. First-generation regenerants with the Cre-loxP system were obtained by selection on kanamycin media. Following a heat treatment, second generation regenerants were screened for excision by PCR using nptII, cre, and T-DNA borders primers. Excision efficiency appeared to be at 4.7% depending on the heat treatment. The footprint of the excision was shown by sequencing between T-DNA borders to correspond to a perfect recombination event. Selectable marker-free sprouts were also obtained from tubers of transgenic events when submitted to similar heat treatment at 4% frequency. Spontaneous excision was not observed out of 196 regenerants from untreated transgenic explants. Biosafety concerns are minimized because the expression of cre gene driven by the hsp70 promoter of Drosophila melanogaster was remarkably low even under heat activation and no functional loxP site were found in published Solanum sequence database. A new plant transformation vector pCIP54/55 was developed including a multiple cloning site and the self-excision system which should be a useful tool not only for marker genes in potato but for any gene or sequence removal in any plant.
Anti-Bacterial Agents↗
Effect of cisplatin treatment on speckled distribution of a serine/arginine-rich nuclear protein CROP/Luc7A.
The C-half of cisplatin resistance-associated overexpressed protein (CROP), an SR-related protein, comprises domains rich in arginine and glutamate residues (RE domain), and is rich in arginine and serine residues (RS domain). We analyzed the role of the individual domains of CROP in cellular localization, subnuclear localization, and protein-protein interaction. CROP fused with green fluorescent protein, GFP-CROP, localized exclusively to the nucleus and showed a speckled intranuclear distribution. The yeast two-hybrid system revealed that CROP interacted with SF2/ASF, an SR protein involved in RNA splicing, as well as CROP itself. The RE and RS domains were necessary for both the intranuclear speckled distribution and the protein-protein interaction. CROP was phosphorylated by mSRPK1, mSRPK2, and Clk1 in vitro, and when cells were treated with cisplatin the subnuclear distribution of GFP-CROP was changed. These results suggest that cisplatin affects RNA splicing by changing the subnuclear distribution of SR proteins including CROP.
Animals↗
Agronomic aspects and environmental impact of reusing marginal water in irrigation: a case study from Egypt.
Egypt produces approximately 2.4 million m3 of secondary treated wastewater (TWW) annually, used for irrigation directly or indirectly by blending with agricultural drainage water (BDW). The annual re-use of (BDW) is approximately 4 million m3. The safe and efficient use of marginal water (BDW and TWW) is a core objective of this study which has been operating from 1997 to date. After six growing seasons the main results can be summarized as follows: MAXIMIZING CROP PRODUCTION: TWW can be used for high production of oil crops (canola, soybean sunflower or maize) compared to fresh water, while BDW can be used for high production of tolerant crops (cotton and sugar beet). CROP QUALITY: using marginal water increases the concentration of elements (Pb, B, Ni, Co) in all crops but these elements were under critical levels (there were no toxicity hazards). It is better to use alternative irrigation with fresh water under a drip irrigation system to maximise crop production and minimise the adverse effects of such water in field crops quality. SOIL POLLUTION AND SALINITY BUILD UP: A drip irrigation system under alternative irrigation by fresh with TWW or BDW reduces salinity build up risks and the levels of elements (Pb, B, Ni, Co) in soil compared to re-use marginal water. SOIL PATHOGENS: Using marginal water slightly contaminated the soil with total faecal coliform (TFC), mites, shigella and salmonella. PLANT ANATOMY: No great changes in anatomical disturbance where induced in different structures of plants which were reduced at maturity stage. PRIMARY GUIDELINES FOR RE-USING MARGINAL WATER: From obtained results it can be recommended to use marginal water with salinity content ranged between 1.1 to 3.64dS/m, and elemental contents (Pb 3.0-3.51 ppm), (B 0.05-1.67 ppm), (Co 0.04-0.07 ppm), (Ni 0.08-0.15 ppm) for safe (field, vegetable and medicinal) crops production. REUSE BIO SOLIDS FOR CROP PRODUCTION: Sewage sludge produced from treated wastewater can be safely used by mixing with rice straw (1:1 w/w) for economic crop production and saving mineral fertilisers. GENERAL CONCLUSION: In the North Nile Delta, marginal water (especially BDW) can be safely used without significant negative impact on the environment, but there is a need for multi-disciplinary, long-term research to investigate irrigation with marginal water in terms of the environment, public health and agricultural productivity.
Agriculture↗
A chamberless field exposure system for determining the effects of gaseous air pollutants on crop growth and yield.
A modification of the chamberless Zonal Air Pollution System (ZAPS) has been developed to permit the exposure of crops to various regimes of exposure to gaseous air pollutants under true field conditions. It provides 12 different levels and patterns of pollutant exposure, simulating a wide range of ambient conditions. The different levels and patterns of exposure are achieved through different rates of discharge of pollutant-enriched gas through manifolds suspended above individual plots. A feature of the layout is that, since the plots are grouped together in blocks of four, wind speed and direction dictate the actual exposure patterns received in any plot. For studies with ozone, O(3), enrichment of the ambient air occurs daily between 0700 and 2059 h (PDT). The overall O(3) concentration supplied to the manifolds is maintained proportional to that in the ambient air, except during the first 3 and the last 3 h of each enrichment period when enrichment is slowly increased and decreased, respectively, to provide smooth transitions from and to the ambient level. Additional control progressively reduces enrichment at low wind speeds. Over a season, the ZAPS results in typical unimodal distributions of concentrations on each plot, with good vertical mixing and reasonable horizontal distributions achieved by the large numbers of discharge orifices in the manifolds. Summary results from two seasons' experiments with processing peas are presented to provide examples of the use of the ZAPS to determine crop yield responses to ozone, based on simple linear regressions of yield against two different exposure indices.
Journal Article↗
Stable genetic transformation of tomato plastids and expression of a foreign protein in fruit.
Transgenic chloroplasts offer unique advantages in plant biotechnology, including high-level foreign protein expression, absence of epigenetic effects, and gene containment due to the lack of transgene transmission through pollen. However, broad application of plastid genome engineering in biotechnology has been largely hampered by both the lack of chloroplast transformation systems for major crop plants and the usually low plastid gene expression levels in nongreen tissues such as fruits, tubers, and other storage organs. Here we describe the development of a plastid transformation system for tomato, Lycopersicon esculentum. This is the first report on the generation of fertile transplastomic plants in a food crop with an edible fruit. We show that chromoplasts in the tomato fruit express the transgene to approximately 50% of the expression levels in leaf chloroplasts. Given the generally very high foreign protein accumulation rates that can be achieved in transgenic chloroplasts (>40% of the total soluble protein), this system paves the way to efficient production of edible vaccines, pharmaceuticals, and antibodies in tomato.
Biotechnology↗
Lotus japonicus as a platform for legume research.
The major role of 'model plants' is to provide knowledge and technologies obtained in related systems to researchers studying crop plants. Lotus japonicus was chosen as a model system first for legume genetics and then for legume genomics. A large number of L. japonicus mutants that have alterations in legume-specific phenomena have been generated and phenotypically characterized, and genomics has drastically accelerated the molecular characterization of these mutants. Substantial resources of information and experimental materials, including genomic and cDNA sequences, corresponding DNA libraries and high-density linkage maps demonstrate that L. japonicus is an excellent model system. Transfer of knowledge from L. japonicus to other legumes, especially crop legumes, is a matter for urgent consideration.
Chromosome Mapping↗
Sunflower (Helianthus annuus L.).
Sunflower (Helianthus annuus L.) is considered one of the recalcitrant species in terms of transformation and regeneration. A routine transformation system of this crop requires competent cell cultures for efficient plant regeneration as well as an effective method for gene delivery. A transformation system was developed by an Agrobacterium tumefaciens-mediated method using split mature embryonic axis explants from the Ha89 genotype. Mean transformation efficiency obtained (measured as PCR+ plants/treated explants) varied from 1 to 5.2% depending on the use of the EHA105 or the C58 strain containing a plasmid with a gene of agronomic interest. The system developed has applicability to several Agrobacterium strains and plasmids with both reporter genes or genes of agronomic interest. Plants obtained with this protocol were confirmed by PCR and Southern blot. Stable inheritance of transgenes was successfully followed until generation T4 in several independent lines.
Agrobacterium tumefaciens↗
Greenhouse gases in intensive agriculture: contributions of individual gases to the radiative forcing of the atmosphere
Agriculture plays a major role in the global fluxes of the greenhouse gases carbon dioxide, nitrous oxide, and methane. From 1991 to 1999, we measured gas fluxes and other sources of global warming potential (GWP) in cropped and nearby unmanaged ecosystems. Net GWP (grams of carbon dioxide equivalents per square meter per year) ranged from 110 in our conventional tillage systems to -211 in early successional communities. None of the annual cropping systems provided net mitigation, although soil carbon accumulation in no-till systems came closest to mitigating all other sources of GWP. In all but one ecosystem, nitrous oxide production was the single greatest source of GWP. In the late successional system, GWP was neutral because of significant methane oxidation. These results suggest additional opportunities for lessening the GWP of agronomic systems.
Journal Article↗
The nutrition and health impact of cash cropping in west Africa: a historical perspective.
The impact of cash cropping in West Africa cannot be isolated from its social and historical background. Among the many changes brought to West African economies by cash cropping since the beginning of the century, the present document shows how the extension of trade with European merchants and colonizers created new sets of values and criteria for wealth. Food crops gradually lost their prominent cultural and economics roles to the benefit of export crops or goods. Traditional systems of agricultural production were profoundly disrupted by military actions. They imposed colonial rule and control of trade of tropical crops and goods. Forced labor and compulsory (poorly paid) work assignments were instituted for private and public enterprises: construction of roads, railways, public buildings and plantations. The main justification was the need for cheap labor to cultivate, transport and build roads for the extraction of raw materials. This in turn caused massive migrations from countries such as Burkina Faso (Upper Volta) to Ivory Coast. Cash cropping made systematic collection of taxes possible. An imposition on a per capita basis became the rule and the major incentive of small farmers to engage in commercial farming. Cash cropping made also possible extensive monetarization of West Africa. This results in both favorable and unfavorable effects on the quality of the diet. In profoundly disrupted traditional societies, the diffusion of new consumption patterns was easier and faster. It led to massive food imports of wheat, rice, sugar, alcohol, etc. Cash cropping was (and still is) practiced as a 'mining' agriculture, exhausting soils and deteriorating their fertility for extended periods of time. In the Sudanian and Sahelian zones cash cropping conflicted with the cultivation of grains because peak demands for labor were similar. Therefore, millet and sorghum production declined. Cash cropping was developed in response to the need of European economies for tropical products. However, colonization as a tool to obtain these raw materials was highly inefficient and associated with a considerable misuse of public funds. Together with cash cropping extension was created a network of intermediaries of large European corporations and shopkeepers who would efficiently drain all the earnings from cash cropping away from productive agricultural investments. It made possible the birth of local bourgeoisies and states dedicated almost exclusively to the extraction of a surplus value from the peasantry through cash cropping. It led to a displacement of the role of women; by attributing to them the less profitable food crops, the new production system managed to feed local cities and migrant workers at low cost. Cash cropping provides indirectly a new mean of exploiting women's labor.(ABSTRACT TRUNCATED AT 400 WORDS)
Africa, Western↗
Assessing potential dietary toxicity of heavy metals in selected vegetables and food crops.
Heavy metals, such as cadmium, copper, lead, chromium and mercury, are important environmental pollutants, particularly in areas with high anthropogenic pressure. Their presence in the atmosphere, soil and water, even in traces can cause serious problems to all organisms, and heavy metal bioaccumulation in the food chain especially can be highly dangerous to human health. Heavy metals enter the human body mainly through two routes namely: inhalation and ingestion, ingestion being the main route of exposure to these elements in human population. Heavy metals intake by human populations through food chain has been reported in many countries. Soil threshold for heavy metal toxicity is an important factor affecting soil environmental capacity of heavy metal and determines heavy metal cumulative loading limits. For soil-plant system, heavy metal toxicity threshold is the highest permissible content in the soil (total or bioavailable concentration) that does not pose any phytotoxic effects or heavy metals in the edible parts of the crops does not exceed food hygiene standards. Factors affecting the thresholds of dietary toxicity of heavy metal in soil-crop system include: soil type which includes soil pH, organic matter content, clay mineral and other soil chemical and biochemical properties; and crop species or cultivars regulated by genetic basis for heavy metal transport and accumulation in plants. In addition, the interactions of soil-plant root-microbes play important roles in regulating heavy metal movement from soil to the edible parts of crops. Agronomic practices such as fertilizer and water managements as well as crop rotation system can affect bioavailability and crop accumulation of heavy metals, thus influencing the thresholds for assessing dietary toxicity of heavy metals in the food chain. This paper reviews the phytotoxic effects and bioaccumulation of heavy metals in vegetables and food crops and assesses soil heavy metal thresholds for potential dietary toxicity.
Biological Availability↗
Concepts and applications of trap cropping in pest management.
Interest in trap cropping, a traditional tool of pest management, has increased considerably in recent years. In this review we propose a broader definition of trap cropping that encompasses the inherent characteristics of the trap crop plants themselves as well as the strategies associated with their deployment. Inherent characteristics of a trap crop may include not only natural differential attractiveness for oviposition and feeding, but also other attributes that enable the trap crop plants to serve as a sink for insects or the pathogens they vector. Successful deployment of trap crops within a landscape depends on the inherent characteristics of the trap crop and the higher value crop, the spatial and temporal characteristics of each, the behavior and movement patterns of insect pests, and the agronomic and economic requirements of the production system. Thus, trap cropping is more knowledge-intensive than many other forms of pest management. We review recent references on trap cropping, classify them according to their modalities and level of implementation, and provide a synthesis of the factors that influence the success of trap cropping. Last, we provide a list of recommendations and guidelines that should prove helpful in moving trap cropping forward to its full potential.
Agriculture↗
Comparing the impact of conventional pesticide and use of a transgenic pest-resistant crop on the beneficial carabid beetle Pterostichus melanarius.
The potential impact of a chemical pesticide control method has been compared with that of transgenic plants expressing a protease inhibitor conferring insect resistance by utilising a tritrophic system comprising the crop plant Brassica napus (L.) (Oilseed rape), the pest mollusc Deroceras reticulatum (Müller) and the predatory carabid beetle Pterostichus melanarius (Illiger). Cypermethrin, as the most widely used pesticide in UK oilseed rape (OSR) cultivation, was selected as the conventional treatment. OSR expressing a cysteine protease inhibitor, oryzacystatin-1 (OC-1), was the transgenic comparator. In feeding trials, D. reticulatum showed no significant long-term effects on measured life history parameters (survival, weight gain, food consumption) as a result of exposure to either the cypermethrin or OC-1 treatment. However, D. reticulatum was able to respond to the presence of the dietary inhibitor by producing two novel proteases following exposure to OC-1-expressing OSR. Similarly, P. melanarius showed no detectable alterations in mortality, weight gain or food consumption when feeding on D. reticulatum previously fed either pesticide-contaminated or GM plant material. Furthermore, as with the slug, a novel form of protease, approximately M(r) 27 kDa, was induced in the carabid in response to feeding on slugs fed OC-1-expressing OSR.
Animals↗
Quantitative real-time PCR assay for determining transgene copy number in transformed plants.
The development of transgenic events can be limited by many factors. These include expression levels, insert stability and inheritance, and the identification of simple insertion events. All of the factors can be related to the copy number of the transgene. Traditionally, copy number has been determined by laborious blotting techniques. We have developed an alternative approach that utilizes the fluorogenic 5' nuclease (TaqMan) assay to quantitatively determine transgene copy level in plants. Using this assay, hundreds of samples can be analyzed per day in contrast to the low throughput encountered with traditional methods. To develop the TaqMan copy number assay, we chose to utilize our highly efficient Agrobacterium-mediated transformation system of maize. This transformation procedure generates predominantly low copy number insertion events, which simplified assay development. We have also successful applied this assay to other crops and transformation systems.
Computer Systems↗
Reproductive disorders of crossbred dairy cows in the central highlands of Ethiopia and their effect on reproductive performance.
The study was conducted to estimate the proportion of reproductive disorders and to determine factors affecting reproductive performance of crossbred dairy cows under four different production systems in the central highlands of Ethiopia. The principal postpartum reproductive disorders were retained fetal membranes (14.7%) and uterine infection (15.5%). Anoestrus was the major postpartum reproductive problem in the mixed crop-livestock production system (38.6%) and was significantly associated with this production system. Apart from anoestrus, the occurrence of reproductive disorders was not significantly associated with a production system. Most of the reproductive disorders occurred as a complex rather than as a single abnormality. Two or more abnormal conditions were seen in 11.4% of the cases. Each reproductive trait measured was affected adversely by reproductive disorders. Cows with reproductive disorders in each production system, lactation group and suckling and non-suckling group had longer intervals from calving to first service and to conception (p < 0.001) and required more services per conception (p < 0.001). Pregnancy rate and conception to first service were 84.7% and 51.7%, respectively, for cows without reproductive health problems; and 64.2%, and 15.1%, respectively, for cows with reproductive disorders (p < 0.001). Overall, intervals from calving to first service were shorter (p < 0.05) than in younger cows. Intervals from calving to first service and to conception were longer in suckling than in non-suckling cows (p > 0.05). Cows with a good body condition score (> 3.5) at calving had shorter calving to first service and conception intervals than cows in poor condition (p < 0.001). The results showed that reproductive abnormalities, coupled with poor body condition, are important factors that contributed to reproductive inefficiency. An appropriate reproductive health management, a reliable artificial insemination service and supplementary feeding could be the management options to reduce or alleviate some of the problems.
Anestrus↗
[Digital modeling of light distribution within the crop canopy in jujube-wheat interplanting system].
On the basis of field observation and with the principles and methods of agricultural meteorology, the daily variations of the shadow width of jujube tree belts and the relative light intensity were analyzed in the jujube-wheat interplanting system, and the characteristics of vertical distribution of the light within the wheat canopy inside and outside the shadow were studied. The sunshine vertical distribution curves in the wheat canopy inside and outside the shadow were simulated by mathematical methods. The results showed that the shadow area of the south-north planting jujube tree belts was fairly wider, but the relative light illuminance was larger in early morning and late afternoon than at about noon. Although the sunshine vertical distribution in the wheat canopy inside and outside the shadow was obviously different, they both observed the decreasing law for Gauss Curve.
Crops, Agricultural↗
Impact of land use practices on faunal abundance, nutrient dynamics and biochemical properties of desert pedoecosystem.
Increased dependence of resource-poor rural communities on soils of low inherent fertility are the major problem of desert agroecosystem. Agrisilviculture practices may help to conserve the soil biota for maintaining essential soil properties and processes in harsh climate. Therefore, the impacts of different land use systems on faunal density, nutrient dynamics and biochemical properties of soil were studied in agrisilviculture system of Indian desert. The selected fields had trees (Zizyphus mauritiana, Prosopis cineraria, Acacia nilotica) and crops (Cuminum cyminum, Brassica nigra, Triticum aestivum) in different combinations. Populations of Acari, Myriapoda, Coleoptera, Collembola, other soil arthropods and total soil fauna showed significant changes with respect to different land use practices and tree species, indicating a strong relation between above and below ground biodiversity. The Coleoptera exhibited greatest association with all agrisilviculture fields. The Z. mauritiana system indicated highest facilitative effects (RTE value) on all groups of soil fauna. Soil temperature, moisture, organic carbon, nitrate- and ammonical-nitrogen, available phosphorus, soil respiration and dehydrogenase activity were greater under tree than that of tree plus cropping system. It showed accumulation of nitrate-nitrogen in tree field and more utilization by crops in cultivated lands. Positive and significant correlation among organic carbon, nitrate- and ammonical-nitrogen, phosphorus, soil respiration and dehydrogenase activity clearly reflects increase in soil nutrients with the increase in microbial and other biotic activity. P. cineraria field was the best pedoecosystem, while C. cyminum was the best winter crop for cultivation in desert agroforestry system for soil biological health and soil sustainability. The increase in organic carbon, soil nutrients and microbial activity is associated with the increase in soil faunal population which reflect role of soil fauna in fertility building. This suggests that strategies may be developed for nurturing fertility-building soil fauna and managing degraded pedoecosystem in desert just by adopting suitable agricultural practices.
Agriculture↗
Controlling internal parasites in grazing ruminants without recourse to anthelmintics: approaches, experiences and prospects.
Interest in ways of raising stock without using anthelmintics has been stimulated by the desire for intensive grazing systems to adopt more sustainable methods of internal parasite control and by potential premiums for "organic" produce. This requires grazing management systems which are practicable and achieve levels of parasite control sufficient to meet realistic production objectives. This paper will summarise 3 years experience of lamb and cattle production without a recourse to anthelmintics on 2 production systems: a mixed cropping, lamb finishing, and cattle rearing unit and an all grass, mixed-stock, hill country unit in which all non-replacement lambs are sold at weaning. On both units parasite control was almost entirely dependent on integrated grazing management of sheep and cattle and strict systems of grazing management had to be rigidly applied. Acceptable productivity could be more readily achieved in sheep than cattle. Rams selected for resistance to nematodes were also used in both sheep flocks. Their influence on lamb production was equivocal. There is a need for more information on factors influencing parasite epidemiology and for consideration of strategies other than alternate grazing. The impact of pasture species on parasite epidemiology needs to be clarified. Current research indicates substantial differences between grasses in terms of parasite burdens acquired and production losses suffered by lambs grazing them. Grazing management may need to vary with pasture species. Also, specialty forage crops, particularly those containing condensed tannins (i.e. Hedysarum coronarium, Lotus pedunculatus and Lotus corniculatus) hold special promise as a means of countering parasite-induced production losses and dagginess. Using biological control of free-living larval stages and vaccination, may in the long term, also prove useful. Developing effective and acceptable systems for raising stock without using anthelmintics presents a considerable challenge, to parasitologists, as well as to plant breeders, agronomists, and farming systems researchers.
Animal Husbandry↗