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Results for “Complement Fixation Tests”

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PubMed1991

Chlamydia pneumoniae respiratory tract infection: the interpretation of high titres in the complement fixation test.

Sera from 5 acute respiratory disease patients from Northern Norway presenting with high chlamydia complement fixation (CF) titres, were analyzed for Chlamydia pneumoniae-specific IgG and IgM in a micro-immunofluorescence (micro-IF) test that included antigens from the prototype strain TW 183, and a Norwegian isolate, FML 10. The patients were confirmed to have had C. pneumoniae infection based on serologic findings. This establishes the clinical significance of early CF results in this geographical area. However, different micro-IF titres against the 2 C. pneumoniae isolates tested suggest antigenic variability among the species.

Adolescent↗
PubMed1975

Complement fixation test as an aid in the diagnosis of farmer's lung.

Complement-fixing antibodies to Micropolyspora faeni were measured in farmers and controls. Farmers with known or suspected farmer's lung showed significantly higher titers than control groups. Variations in titer were measured and correlated with the clinical disease.

Antibodies, Bacterial↗
PubMedDate not supplied

Complement-fixation test for reticuloendotheliosis viruses: limits of sensitivity in infected avian cells.

A specific micro-complement-fixation procedure for assay of avian reticuloendotheliosis viruses (REV), designated by use as the COFAR test, was compared with an assay based on immunofluorescent (IF) antibody staining of infected chick embryo fibroblasts. Endpoint titrations in which REV strain T, chick syncytial virus, and spleen necrosis virus were used indicated that cultures infected with limiting dilutions of each strain were positive by both procedures within 6 days. Depending on cell density, infection of 2 to 9% of the cells cultured produced an unambiguous positive response (titer greater than or equal to 1:2) with COFAR test. When both tests were used in a study on the transmission and in vivo status of ducks infected with spleen necrosis virus, COFAR was no less, possibly more, sensitive than IF for detecting infection in cultures inoculated with plasma.

Animals↗
PubMed1985

[Serologic diagnosis of primary cytomegalovirus infections in patients following kidney transplantation using the indirect immunofluorescence technic and the complement-fixation test].

Sera of 22 recipients of kidney transplants with clinically and serologically (complement binding reaction) ascertained cytomegalovirus infection were retrospectively examined for virus-specific antibodies of the class IgM by means of the indirect immunofluorescence test. Already two to five days after the appearance of the adequate clinical symptoms (leucopenia, fever, T-lymphocytopenia, increase of creatinine and thrombocytopenia) CMV-IgM-antibodies could be proved. On average a positive complement binding reaction was found only two weeks later. In other 13 patients of the actual control programme the CMV-IgM-antibody findings first obtained were compared with the results of the complement fixing reaction obtained later. Neither falsely positive nor falsely negative results could be found.

Antibodies, Viral↗