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Changes in bone mineral density of lumbar vertebrae after parturition in African green monkeys (Cercopithecus aethiops).

We examined the effect of lactation on the maternal bone components in laboratory-bred African green monkeys. Bone mineral density (BMD) with bone mineral content (BMC), bone area and mean width of lumbar vertebrae (L3-L5) were measured by dual-energy X-ray absorptiometry (DXA). Measurements were conducted at two-week intervals from parturition up to 40 weeks after parturition. The BMC and BMD values gradually decreased during the lactation period and then gradually increased after weaning, but the BMC and BMD of the maternal lumbar vertebrae did not recover completely to the values at parturition even at 40 weeks after parturition.

Absorptiometry, Photon

Changes in normal vaginal flora of African green monkeys (Cercopithecus aethiops) during the menstrual cycle.

Changes in normal vaginal flora of African green monkeys associated with the estrous cycle were examined by the swab method. Bacteroidaceae, corynebacteria, and streptococci were predominant throughout the whole estrous cycle, although individual differences were great. It was also clear that all bacterial species tended to decrease in the menstrual phase. Lactobacilli, the most predominant bacteria in the normal vagina of humans, were detected only in very low numbers in the African green monkey, suggesting that the normal vagina of the African green monkey has a different ecosystem from that of normal human vaginal flora.

Animals

[Age-related changes in bone mineral density, mean width and area of the lumbar vertebrae in male African green monkeys (Cercopithecus aethiops)].

We were able to measure bone mineral density (BMD), mean bone width and the area of the lumbar vertebrae in male African green monkeys with a dual-energy X-ray absorptiometry (DPX-alpha, Lunar, USA). For analysis of spinal bone mass, mean width and area of the bone, we scanned lumbar vertebrae L3-L5 using a pediatric analysis program. We used restraining bed made of formed polystyrol to support the animal in a supine position. The precision of the measuring technique was calculated by averaging the coefficients of variance of the 5 scans obtained in 5 animals aged from 5 to 7 years. The precision values for BMD, bone width and area were 1.9%, 0.9% and 2.6%, respectively. Furthermore, age-related changes in bone measurements were demonstrated using laboratory-bred males (n = 45) and, additionally, wild-originated males (n = 7, estimated ages over 15 years old). The values of B M D, bone width and area at 6 months old were about 0.3 g/cm2, 1.2cm and 4.5 cm2 respectively. These values increased with aging to about 0.7 g/cm2, 1.5 cm and 11cm2 at 5 years old, respectively, and were judged to reach plateau. However, animals over 10 years old and wild-originated animals which had been kept over 10 years in our breeding colony showed rather smaller BMD and area values than the animals aged 5 to 10 years. The animals aged between 5 and 10 years seemed to have retained a peak bone mass in this primate species.

Absorptiometry, Photon

[Relationship between serum biochemical measurements and spinal bone mineral density in African green monkeys (Cercopithecus aethiops)].

Spinal bone mineral density (BMD) of African green monkeys were measured by a dual-energy X-ray absorptiometry (DEXA). The value determined in male was rather higher (0.674 +/- 0.068g/cm2, Mean +/- S. D.) than that in female (0.611 +/- 0.105g/cm2), while the coefficient of variance was larger in female (17%) than in male (10%). Females were divided into two groups according to their diversity of BMD values. Serum biochemical values were compared among three groups, one male group (n = 37) and two groups of female (A: n = 31, B: n = 22), using a canonical discriminant analysis. As the result of the canonical discriminant analysis, closed relationship between serum biochemical properties and BMD was demonstrated. Lower BMD, lower serum protein concentration and higher serum inorganic phosphorus may be the reflection of malnutrition in this primate species. In conclusion, animals showing osteopenia were included in our breeding colony of the African green monkey judging by the value of bone measurements and serum biochemical measurements.

Absorptiometry, Photon

[Changes in bone mineral density of lumbar vertebrae during a six-month interval in African green monkeys (Cercopithecus aethiops)].

Changes in measurements of lumbar vertebrae (L3-L5) during a six-month interval in African green monkeys were demonstrated using dual-energy X-ray absorptiometry (DEXA, DPX-alpha, Lunar, USA). Area, mean width and bone mineral content (BMC) of L3-L5 were measured, and bone mineral density (BMD) was calculated using 62 laboratory-bred and 23 wild animals. Laboratory-bred animals were divided into 4 groups according to their gender and age, and in addition, two groups of wild animals were constructed according to gender. Although no remarkable change in BMD of laboratory-bred animals aged less than two years was observed at a six-month interval, significant decreases in BMD were detected in laboratory-bred animals aged over 6 years as well as in wild animals. Thus, DEXA seems to be useful for investigating the kinetics of BMD in aged animals during a relatively short period such as six-months.

Absorptiometry, Photon

Changes in WGA-lectin binding sites on sperm during epididymal transit in the Chacma baboon (Papio ursinus) and the vervet monkey (Cercopithecus aethiops).

Epididymal maturation of sperm entails molecular changes to the sperm plasma membrane. These changes are reflected by changes in lectin binding. The wheat germ agglutinin (WGA) lectin was used, since it binds to acetylglucosamine and sialic acid, which are involved in sperm function. The objective was to study changes in WGA-lectin binding during epididymal passage and in the ejaculate of the Chacma baboon and vervet monkey. The intensity and location of lectin binding on sperm from the ejaculate and the caput, corpus, and cauda epididymidis of 5 adult males from each species were studied. In both baboon and monkey sperm, the acrosomal region had the highest intensity stain and the equatorial region had the lowest intensity. Significant differences in staining of the different regions of sperm were found for the different epididymal sites and the ejaculate of both species. It was concluded that changes in the molecular composition of the sperm membrane, taking place during epididymal maturation, can at least in part be monitored by studying WGA-lectin binding on sperm. The changes noted are probably important for the organization of a membrane of the correct molecular conformation and mandatory for complete sperm function.

Animals

Experimental louse-borne relapsing fever in the grivet monkey, Cercopithecus aethiops. III. Crisis following therapy.

Eight grivet monkeys infected with Borrelia recurrentis received tetracycline (12.5mg/kg body weight) on the 3rd or 4th day of spirochetemia. Leukopenia, fever, hyperpnea, and tachycardia developed within 2 hours as spirochete counts fell to undetectable levels. These events closely simulated the crisis in human louse-borne relapsing fever in both timing and extent.

Animals