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Corn silage hybrid effects on intake, digestion, and milk production by dairy cows.

Three corn hybrids harvested as whole-plant silage were evaluated in three separate feeding trials with lactating dairy cows. In trial 1, 24 multiparous Holstein cows were used in a replicated 4 x 4 Latin square with 28-d periods. Treatments were conventional (Pioneer 3563) and leafy (Mycogen TMF 106) corn silage hybrids, each planted at low (59,000 plants/ha) and high (79,000 plants/ha) plant populations. There were no milk production differences between treatments. Total-tract digestibility of dietary starch was higher for leafy compared with conventional corn hybrids. In trial 2, 26 multiparous Holstein cows were assigned randomly to diets containing either conventional (48% forage diet) or brown-midrib (60% forage diet) corn silage in a crossover design with 8-wk periods. Milk yield was lower, but milk fat percentage and yield were higher, for the high-forage diet containing brown-midrib corn silage. In trial 3, 24 multiparous Holstein cows were used in a replicated 4 x 4 Latin square with 28-d periods. Treatments were corn silage at two concentrations of neutral detergent fiber (Garst 8751, 39.2% NDF; Cargill 3677, 32.8% NDF) each fed in normal- (53% of dry matter) and high- (61 to 67% of dry matter) forage diets. Milk production was not different between corn hybrids. Increased concentrate supplementation increased DMI and milk production. There were minimal benefits to the feeding of leafy or low-fiber corn silage hybrids. Feeding brown-midrib corn silage in a high-forage diet increased milk fat percentage and yield compared with conventional corn silage fed in a normal-forage diet.

Animals↗

Site of nutrient digestion by dairy cows fed corn of different particle sizes or steam-rolled.

Five primiparous Holstein cows were cannulated in the rumen, duodenum, and ileum and were fed diets containing 50% alfalfa silage and 36.6% coarse-, medium-, or fine-ground corn (CGC, MGC, and FGC, respectively, with mean particle sizes of 4.8, 2.6, or 1.2 mm), steam-rolled corn (SRC; density of 0.53 kg/L), or a 50:50 mix of CGC and SRC (SC) to evaluate how corn processing affects site of digestibility of nonstructural carbohydrates (NSC) and neutral detergent fiber (NDF). Decreasing the particle size of corn quadratically affected true ruminal digestibility of NSC (49.8, 46.5, and 87.0%, respectively). Because of compensatory digestion postruminally, a smaller increase (from 91.3 to 98.0%) in total tract digestibility of NSC was noted as particle size decreased. A small but significant linear shift in NDF digestion from the rumen to the large intestine was detected as corn particle size decreased. The addition of SRC to CGC linearly increased true NSC digestibility in the rumen about 20 percentage units but had much smaller effects on total tract digestibility. Despite the large impact of corn processing on NSC digestibility in the rumen, flow of bacterial N to the rumen was not affected by treatment. Reducing the particle size of corn decreased the apparent escape of corn protein, but steam-rolling had no effect. Corn should be finely ground to maximize total tract OM digestibility or steam-processed to densities less than 0.53 kg/L for maximal starch digestibility. However, fine-grinding or steam-processing of corn may have only a modest impact on total tract OM digestibility.

Animals↗

Ruminal fermentation and intestinal flow of nutrients by lactating cows consuming brown midrib corn silages.

Rumen characteristics and digestive kinetics of brown midrib corn silage were evaluated with five late-lactation (221 DIM +/- 20 d) multiparous cows fitted with ruminal and duodenal cannula. Dietary treatments were applied by using a single reversal design with two 21-d periods where either brown midrib (BM3) or isogenic (ISO) corn silage were included in a total mixed ration formulated to be 40% concentrate and 60% corn silage on a dry matter (DM) basis. Rumen and total tract digestibilities of DM, organic matter, neutral detergent fiber, acid detergent fiber, starch, and N were determined and rumen characteristics evaluated. Apparent rumen DM and organic matter digestibilities were greater for the BM3 corn silage (7.1 and 4.7 percentage units, respectively). Dietary intake and duodenal flow of starch were greater and rumen and total tract starch digestibilities were lower for BM3 corn silage diets than ISO corn silage diets. However, more starch (1.1 kg/d) was apparently digested and absorbed postruminally in cows fed the BM3 corn silage diets. Duodenal flow of neutral detergent fiber was 0.9 kg/d lower, and ruminal (15.9 percentage units) and total tract digestibilities (4.4 percentage units) were higher for BM3 treatment compared with the ISO treatment. Digestive patterns of ADF were similar for the BM3 and ISO treatments. Ruminal pH was lower in the cows fed the BM3 corn silage than those fed the ISO corn silage. As a result of a tendency for decreased N excretion in urine and slight increases in N intake due to increased DM intake, N balance tended to be greater for the BM3 treatment compared with the ISO treatment. These results may partially explain the benefits of feeding BM3 corn silage to cows during early lactation, as the observed increases in fiber component digestibility and improved N economy may combine to enhance DM intake and better support the nutritional demands of milk production for the high producing dairy cow.

Animal Nutritional Physiological Phenomena↗

Corn silage management: effects of maturity, inoculation, and mechanical processing on pack density and aerobic stability.

Three experiments were conducted to evaluate the effects of inoculation, maturity, and mechanical processing of corn silage on aerobic stability and pack density. Corn silage was stored in 20-L mini silos for the three aerobic stability experiments. Corn silage was stored in 80-L mini silos for the three pack-density experiments. The wet pack density of corn silage tended to decrease as maturity advanced in all of the pack-density experiments, and processed corn silage had a greater wet pack density compared with unprocessed corn silage in two of the three 20-L mini silo experiments. Aerobic stability, measured as the number of hours to reach 1.7 degrees C above ambient, was greater for processed corn silage in two of the three 20-L mini silo experiments, and was greater for inoculated corn silage across the three 20-L mini silo experiments. Inoculation of corn silage with lactic acid producing bacteria tended to improve aerobic stability of corn silage more than maturity and mechanical processing.

Aerobiosis↗

Corn silage management: effects of hybrid, maturity, chop length, and mechanical processing on rate and extent of digestion.

Five experiments were conducted to evaluate the effects of hybrid, chop length, maturity, and mechanical processing of corn silage on dry matter and nutrient disappearance in the rumen. Corn silage that had not been dried or ground was incubated in macro in situ bags (30 x 35 cm) for 8, 16, 24,48, and 96 h. Experiments la and 2 evaluated the effects of maturity and mechanical processing for two corn silage hybrids harvested at two theoretical lengths-of-cut. Experiments 3 through 5 evaluated the effects of chop length and mechanical processing for two corn silage hybrids harvested at two-thirds milkline. The hybrid with low neutral fiber (NDF) concentrations had greater dry matter and starch disappearance than the hybrid with high NDF concentrations. The effect of chop length on nutrient disappearance was variable across experiments 3 through 5. Processing improved dry matter disappearance in experiments la, 2 (two-thirds milkline and blackline), 3, 4, and 5 at the majority of ruminal incubation timepoints. Starch disappearance was greater for unprocessed corn silage in experiment la (hard dough and two-thirds milkline) and was greater for processed corn silage in experiments 2 and 5. However, there was no consistent trend in starch disappearance between processed and unprocessed corn silage in experiments 3 and 4. This can be partially explained by the high disappearance of starch (experiment 3, > or = 98% and experiment 4, > or = 94%) by 24 h of ruminal incubation. Minimal differences were detected in NDF disappearance between processed and unprocessed corn silage across maturities. In experiments 2 and 5, crude protein disappearance was improved due to processing at some incubation timepoints. Rate of dry matter, starch (one-third milkline and two-thirds milkline), and NDF disappearance tended to increase when corn silage was mechanically processed in experiment 2. Dry matter, starch, and crude protein disappearance tended to be greater for corn silage harvested at the early maturity (one-third milkline) in experiment 2 compared with advanced maturities (two-thirds milkline and blackline). Disappearance of NDF was greater at early maturities compared with advanced maturities in experiments 1a and 2. Rate of dry matter (hybrid 3845), starch, and NDF (hybrid 3845) disappearance tended to decrease as maturity advanced from one-third milkline to blackline in experiment 2. These results suggest that the macro in situ method can be used to evaluate nutrient disappearance in the rumen.

Animals↗

The energy content of wet corn distillers grains for lactating dairy cows.

Forty-five energy balances were completed with 12 multiparous, lactating Holstein cows in a study designed to determine the energy content of wet corn distillers grains. Treatments were applied in a repeated switchback design and consisted of total mixed diets containing 31.4% corn silage, 18.4% alfalfa hay, and either 30.7% rolled corn and 16.7% soybean meal or 17.0% rolled corn and 31.2% wet corn distillers grains (dry matter basis). Replacement of corn and soybean meal with wet corn distillers grains reduced dry matter intake 10.9% but did not affect milk production. Neither digestible nor metabolizable energy were affected by diet composition. Heat and milk energy output did not differ by diet, but body energy retained was 2.8 Mcal/d less in cows fed the wet corn distillers grains diet. Multiple regression estimates of maintenance metabolizable energy requirement and partial efficiencies of metabolizable energy used for lactation and body energy deposition did not differ by diet. Pooled estimates were 136.2, 0.66, and 0.85, kcal of metabolizable energy/ body weight0.75 per day, respectively. Calculated by difference, wet corn distillers grains was estimated to contain 4.09, 3.36, and 2.27 Mcal/kg of dry matter as digestible, metabolizable, and lactational net energy, respectively. These energy estimates were 7 to 11% and 10 to 15%, respectively, greater than those reported for dried corn distillers grains by the 1989 and 2001 dairy NRC publications.

Animal Feed↗

Effect of steam-flaked or steam-rolled corn with or without Aspergillus oryzae in the diet on performance of dairy cows fed during hot weather.

The objective of this study was to determine the effects of steam-rolled versus steam-flaked corn in the diet with or without the addition of a culture of Aspergillus oryzae on the performance of high producing dairy cows during hot summer weather. Thirty-two Holstein cows averaging 92 (+/- 60) d in milk were fed a pretreatment diet for 21 d followed by a 70-d experimental period in a completely randomized block design with a 2 x 2 factorial arrangement of treatments. Diets were 1) steam-flaked corn plus 3 g/d of A. oryzae, 2) steam-flaked corn, 3) steam-rolled corn plus 3 g/d of A. oryzae, and 4) steam-rolled corn. Intake was not affected significantly by grain processing or addition of A. oryzae. Compared with effects from steam-rolled corn in the diet, steam-flaked corn increased milk production; percentage of milk protein; yields of milk protein, lactose, and SNF; and the efficiency of conversion of dry matter to fat-corrected milk. Addition of A. oryzae tended to increase protein percentage and increased the percentage of SNF. Changes in body weight and body condition score tended to be higher, and somatic cell count tended to be lower, for cows fed the flaked corn than for cows fed the rolled corn. No interactions were significant. Treatments did not affect rectal temperatures or respiration rates; however, high mean values measured at 1400 h once weekly indicated thermal stress. These data show improved milk production from cows fed steam-flaked corn but not from those fed diets supplemented with A. oryzae.

Animal Feed↗

The effect of drying method and storage time on citrinin activity in corn.

Sterilized whole corn was inoculated with a Penicillium spp.,3 a citrinin-producing fungus, and incubated at room temperature for 8 weeks. The corn was then blended and divided into two parts, which were dried either in air at room temperature (ADC) or in a forced draft oven at 60 C for 24 hr (ODC). Each batch of corn was ground and stored in plastic containers. The corn was fed to broiler chicks prior to and after 3, 6, 9, and 12 months of storage at 4 C. The inoculated corn was fed undiluted or blended with noninoculated corn to give mixtures containing one-third and two-thirds of inoculated corn. Noninoculated corn, inoculated corn, and blends of the two were fed to chicks for 5 hr as the only feed. Feed consumption, water intake, and excretion were measured at the end of the test. The citrinin contents of the ADC at 3, 6, 9, and 12 mo were 824, 599, 667, and 590 ppm and were 928, 541, 781, and 490 ppm for the ODC at the same periods. Water consumption and water excretion increased as the amount of citrinin intake increased. Neither drying method nor length of storage affected the citrinin activity in the corn. The citrinin in ODC was neither bound nor converted to a derivative that was unextractable.

Animal Feed↗

Nutritional value for growing turkeys of corn of light test weight.

An experiment was conducted to determine the relationship between test weight of corn and growth, efficiency of feed utilization, nutrient retention, and carcass composition of young turkeys. The MEn values of corn were also measured. Two samples of corn differing substantially in test weight were fed to male turkey poults (Nicholas Large White) from 1 to 20 d of age. The test weights of the light-weight corn and the normal-weight corn were 55 kg/hL (43 lb/bu) and 70 kg/hL (54 lb/bu), respectively. The light-weight corn was lower in crude protein concentration (6.63%) than the normal-weight corn (8.53%). The determined MEn of the corn samples did not differ (3.22 vs 3.25 kcal/g). The light-weight corn diet had modest adverse effects on growth and feed efficiency of poults during the 1 to 7 d after hatching. Poult carcass composition and retention of nitrogen and energy were not affected by the test weight of corn.

Animal Feed↗

Stability and problems in recovery of fumonisins added to corn-based foods.

Because the natural occurrence of fumonisins is so far known almost exclusively in corn, we have limited our investigations on their stability to corn-based foods. In these studies, distinction must be made between real losses, binding, and any matrix-related method problems. Fumonisins B1 (FB1) and B2 (FB2) were about 40% recovered when heated in corn meal at 190 degrees C, about 20-30% recovered when heated in moist corn meal at 190 degrees C, and completely unstable in corn meal at 220 degrees C. Average recoveries of FB1 and FB2 added to blank heated matrixes were 69-107% in control experiments. Baking corn meal muffins spiked with 2.5 micrograms FB1 and FB2/g corn meal at 220 degrees C also resulted in losses of fumonisins. Little or no fumonisins were recovered from corn bran flour when methanol-water (3 + 1) was used as extraction solvent. However, when methanol-borate buffer (pH 9.2) (3 + 1) was used, recoveries averaged 91 +/- 17 and 84 +/- 9%, respectively, for FB1 and FB2; and natural contamination of the corn bran flour with FB1 and FB2 at levels of 1.9 and 0.95 microgram/g, respectively, was revealed. Comparable recoveries were observed for 1 brand of a corn bran breakfast cereal, but the binding effect was not seen with a second brand, for which methanol-water (3 + 1) alone was a good extraction solvent. Recoveries of FB1 and FB2 from a mixed cereal for babies were only about 50% with either extraction solvent mixture.(ABSTRACT TRUNCATED AT 250 WORDS)

Drug Stability↗

Interaction between orosensory and postingestional stimulation in the control of corn oil intake by rats.

The microstructure of the ingestive behavior of rats ingesting corn oil emulsions was analyzed to describe quantitatively how postingestional stimulation interacts with orosensory stimulation to control the intake of corn oil. Seventeen hour food deprived rats were given 30-min access intake tests with corn oil emulsions ranging in concentration from 0.125% to 64%. The volume ingested showed an inverted U shaped function over this concentration range. The Weibull function fitted to the rate of licking function showed that the initial rate of licking was an increasing linear function of the log of the concentration of corn oil. The slope of the licking rate function was an increasing curvilinear function of the log of corn oil concentration. Together these results indicate that the negative feedback effect of corn oil in the gastrointestinal tract increases more rapidly at the higher concentrations of corn oil than does its orosensory stimulating effect resulting in a decrease in the volume ingested. Unlike carbohydrates, where the increase in the initial rate of licking is a result of an increase in the size of the clusters, the increase with concentration in the initial rate of ingesting corn oil was caused by an increase in the number of clusters rather than their size. This study provides a quantitative analysis of the interaction between orosensory and postingestional stimulation, two variables that play an important role in the control of ingestion of corn oil.

Animals↗

Opioidergic contribution to conditioned place preference induced by corn oil in mice.

We previously reported that voluntary intake of corn oil in the light box showed place preference in the conditioned place preference (CPP) test in mice. In the present study, we investigated the contribution of opioidergic systems to the corn oil-induced CPP in mice. Acquisition of the place preference by corn oil intake was blocked by i.p. injections of an opioid mu antagonist, naloxone (0.1 and 0.3 mg/kg), and delta antagonists, 7-benzylidenenaltrexone (0.5 mg/kg) and naltriben (0.5 mg/kg) 15 min before conditioning. The opioid kappa agonist U-50488H (1 and 3 mg/kg i.p.) also blocked corn oil-induced CPP. Naloxone (1 mg/kg, i.p.) and naltriben (0.5 mg/kg, i.p.) did not affect corn oil intake in the home cage. However, 7-benzylidenenaltrexone (0.5 mg/kg, i.p.) and U-50488H (1 mg/kg i.p.) decreased and increased the corn oil intake, respectively. These results suggested that the rewarding effects of corn oil in the CPP test are at least partially mediated via opioidergic systems through mu and delta receptors. Further, we showed that an opioid kappa agonist reduced the rewarding effects of corn oil in the CPP test in mice, although it increased corn oil intake.

Animals↗

Performance of commercial laying hens when six percent corn oil is added to the diet at various ages and with different levels of tryptophan and protein.

Three experiments were conducted to evaluate the use of corn oil in the diet of commercial layers as a method of increasing egg weight. In the first experiment, the performance of commercial layers receiving 6% added corn oil beginning at 18 to 30 wk at 2-wk intervals was evaluated. In the second experiment, comparisons were made between performance of young and old commercial layers when 6% corn oil was added to the diet. The third experiment was conducted to evaluate the effects upon commercial layers when 6% corn oil was added to diets varying in Trp content. In experiment 1, egg weights increased during the first 2 wk that corn oil was added to the diet. During this time, the intake of Trp was greater than or equal to the hen requirements. However, during the last 2 wk of the experiment, when Trp intakes were low, egg weights decreased when corn oil was added to the diet. In experiment 2, egg weights from young and old hens increased during the first 2 wk after corn oil was added to the diet. During the last 2 wk, egg weights from young hens decreased, whereas egg weights from old hens increased. In experiment 3, egg weight was not affected when the diet contained 0.166 or 0.176% Trp. However, egg weights significantly increased when corn oil was added to the diet containing 0.193% Trp. The data in these experiments indicate that the diet of a laying hen must contain a high level of Trp to get an increase in egg weight from the addition of corn oil to the diet. Also the need for other amino acids must be met. Therefore, the hen eats to meet her energy requirement for maximum egg production, and her amino acid intake determines the egg weight.

Aging↗

Increased flavor preference and lick activity for sucrose and corn oil in SWR/J vs. AKR/J mice.

Nutrient preferences and orosensory responses were characterized in two mouse inbred strains. In two-bottle solution tests (tastant vs. vehicle; ascending concentrations), the effects of strain and chow type (12 or 26% fat) on preference thresholds for sucrose and corn oil were compared in AKR/J and SWR/J mice. SWR/J mice displayed lower preference thresholds and ingested more sucrose than AKR/J mice did. SWR/J mice also showed lower preference thresholds and consumed more corn oil than AKR/J mice did; corn oil preference was suppressed 3.5-fold in AKR/J mice compared with SWR/J mice when fed 26% fat chow. Next, licking was recorded during 30-s access to sucrose or corn oil across a range of concentrations. SWR/J mice licked the tastants more than AKR/J mice did. Analysis of modal interlick intervals during lick training revealed that SWR/J mice licked water faster than AKR/J mice when water deprived, suggesting that motor as well as sensory factors may determine lick responses to tastants in brief-access tests. Finally, in two-bottle tests pitting maximally preferred concentrations of sucrose (8 or 16%) against corn oil (20%), SWR/J mice highly preferred sucrose over corn oil at either sucrose concentration. AKR/J mice preferred corn oil over 8% sucrose but reversed their preference when 16% sucrose was offered. These results support a primary role of flavor in the nutrient preferences of SWR/J mice. In AKR/J mice, the low lick activity for sucrose and corn oil and greater suppression of corn oil preference by the high-fat chow suggest that their preferences depend more on postingestive factors than on flavor.

Animal Nutritional Physiological Phenomena↗

Diets containing high oil corn and tallow for dairy cows during early lactation.

Four multiparous Holstein cows averaging 49 DIM and fitted with ruminal cannulas were utilized in a 4 x 4 Latin square design with 21-d periods to determine the effects of diets containing high oil corn grain and tallow. Cows were fed diets of alfalfa haylage and concentrate (37:63, DM basis) for ad libitum intake. Treatments were 1) control, no added fat; 2) high oil corn grain replacing regular corn grain; 3) high oil corn grain and 2.5% tallow; and 4) high oil corn grain and 5% tallow. Intake of DM, milk production, and yields of milk fat, milk protein, milk SNF, and 4% FCM were not affected by dietary fat, although DMI tended to be lower when cows were fed 5% tallow. Percentages of protein and SNF in milk were lower when cows were fed diets containing fat. Percentage of milk fat was lower when cows were fed diets containing tallow. Supplemental fat decreased total VFA concentrations in ruminal fluid. Cows fed high oil corn had a greater molar proportion of acetate and a larger acetate to propionate ratio in ruminal fluid than cows fed high oil corn and tallow. Digestibility of total fatty acids decreased when tallow was added to diets containing high oil corn. No differences were observed among treatments for total tract apparent digestibilities of DM and fiber or utilization of energy and N. Dietary high oil corn and 5% tallow tended to decrease DMI and to alter ruminal fermentation characteristics.

Animal Feed↗

Novel approach of corn fiber utilization.

The corn wet milling process produces a 10% (w/w of the processed corn) byproduct called corn fiber, which is utilized worldwide as a low-value feedstock for cattle. The aim of this study was to find a higher value use of corn fiber. The main fractions of corn fiber are: 20% starch, 40% hemicellulose, 14% cellulose, and 14% protein. Extraction of the highly valuable, cholesterol-lowering corn fiber oil is not feasible owing to its low (2% w/w) concentration in the fiber. The developed technology is based on simple and inexpensive procedures, like washing with hot water, dilute acid hydrolysis at 120 degrees C, enzymatic hydrolysis of cellulose, screening, drying, and extraction. The main fractions are sharply separated in the order of starch, hemicellulose, cellulose, lipoprotein, and lignin). The lipoprotein fraction adds up to 10% of the original dry corn fiber, and contains 45% corn fiber oil, thus yielding more oil than direct extraction of the fiber. It is concluded that the defined method makes the extraction of the corn fiber oil economically feasible. The fractionation process also significantly increases the yield of cholesterol-lowering substances (sterols and sterolesters). At the same time clear and utilizable fractions of monosaccharides, protein, and lignin are produced.

Cellulose↗

Corn wet milling: separation chemistry and technology.

Corn wet milling is a complicated, large-scale, and efficient industrial process designed to separate the chemical components from corn kernels. The success of wet milling, in terms of maximum yields, is largely dependent on the success of the steeping process. Improper steeping, or steeping of corn kernels that have unusual physical or chemical structures, results in lost product and lower profits. Steeping processes, however, are still based largely on an art that was developed more than 100 years ago. In the next 20 years, major seed corn producers expect that the market for corn with unique starch characteristics and corn bred to produce speciality chemicals or have an altered composition will expand substantially. As the market increases for speciality corn, corn genetically bred with unique starch charcteristics or corn with altered chemical composition, a thorough scientific understanding of steeping chemistry and the entire wet milling process will become increasingly important.

Absorption↗

Physicochemical changes in the hull of corn grains during their alkaline cooking.

The alkaline cooking of corn in a solution of Ca(OH)2 to produce corn-based foods is oriented to make corn proteins available, to incorporate Ca to the cooked grains, and also to remove the corn hull. This process (nixtamalization) is known in Mexico and Guatemala from prehispanic times; however, the effect of the alkaline cooking on the corn hull remains poorly documented. In this work, the physicochemical changes that take place in the corn hull during its cooking in a saturated solution of Ca(OH)2 were studied using infrared, X-ray diffraction, 13C cross-polarization/magic-angle spinning (CP/MAS) NMR, confocal imaging microscopy, differential scanning calorimetry, and thermogravimetry techniques. The main effect of this treatment on the hull is the removal of hemicelluloses and lignin, increasing the hull permeability and, as a consequence, facilitating the entry of the alkaline solution into the corn kernel. No significant changes were observed in the cellulose fiber network, which remains as native cellulose I, with a crystalline index, according to 13C CP/MAS NMR spectra, of 0.60. The alkaline treatment does not allow the cellulose fibers to swell and their regeneration in the form of cellulose(II). It seems any attempt to make use of the Ca binding capacity of the hull to increase the Ca availability in nixtamalized corn-based foods requires a separated treatment for the hull and kernel. On alkaline cooking, the hull hemicellulose fraction dissolves, losing its ability to bind Ca as a way to incorporate this element into foods elaborated from nixtamalized corn.

Calcium Hydroxide↗