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PubMed1996

Vegetable, fruit, and cereal fiber intake and risk of coronary heart disease among men.

OBJECTIVE: To examine prospectively the relationship between dietary fiber and risk of coronary heart disease. DESIGN: Cohort study. SETTING: In 1986, a total of 43,757 US male health professionals 40 to 75 years of age and free from diagnosed cardiovascular disease and diabetes completed a detailed 131-item dietary questionnaire used to measure usual intake of total dietary fiber and specific food sources of fiber. MAIN OUTCOME MEASURE: Fatal and nonfatal myocardial infarction (MI). RESULTS: During 6 years of follow-up, we documented 734 cases of MI (229 were fatal coronary heart disease). The age-adjusted relative risk (RR) for total MI was 0.59 (95% confidence interval [CI], 0.46 to 0.76) among men in the highest quintile of total dietary fiber intake (median, 28.9 g/d) compared with men in the lowest quartile (median, 12.4 g/d). The inverse association was strongest for fatal coronary disease (RR, 0.45; 95% CI, 0.28 to 0.72). After controlling for smoking, physical activity and other known nondietary cardiovascular risk factors, dietary saturated fat, vitamin E, total energy intake, and alcohol intake, the RRs were only modestly attenuated. A 10-g increase in total dietary fiber corresponded to an RR for total MI of 0.81 (95% CI, 0.70 to 0.93). Within the three main food contributors to total fiber intake (vegetable, fruit, and cereal), cereal fiber was most strongly associated with a reduced risk of total MI (RR, 0.71; 95% CI, 0.55 to 0.91 for each 10-g increase in cereal fiber per day). CONCLUSIONS: Our results suggest an inverse association between fiber intake and MI. These results support current national dietary guidelines to increase dietary fiber intake and suggest that fiber, independent of fat intake, is an important dietary component for the prevention of coronary disease.

Adult↗
PubMedDate not supplied

Chemiluminescence of cereal products II. Chemiluminescence spectra.

Spectra of ultraweak chemiluminescence (CL) accompanying auto-oxidation and hydration of cereal products have been measured using single photon counting and cut-off filters. The spectra cover the 380-880 nm spectral range with maxima centred around 600 nm. Analytically pure air-dried carbohydrates like agar, cellulose and nitrocellulose give emission too weak for spectral measurements. The emission from water pure carbohydrates is on average 4-12 times higher and emission spectra are similar to those from cereal products. The effect of free radical scavengers, SOD and O2* (1 delta g)-quenchers on CL spectra indicates a contribution of radical reactions with the participation of excited carbonyls, O2- and excited molecular oxygen dimoles. Moreover, possible mechanisms of chemi-excitation due to a cooperative H-bond formation during the hydration of carbohydrates and/or recombination of trapped radicals and electron-holes are discussed. It is also postulated that the excitation energy transfer to natural sensitizers occurring in cereal products may account for non-specific broad spectra and differences in the intensity of CL.

Bread↗
PubMedDate not supplied

Chemiluminescence of cereal products III. Two-dimensional photocount imaging of chemiluminescence.

Two-dimensional (2-D) spatial distributions of ultra-weak chemiluminescence (photon imaging) from auto-oxidizing and water-hydrated cereal food products were measured by means of a high-sensitivity 2-D photon counting system--an intensified charge coupled device (CCD) camera. The 2-D images obtained reveal the dynamics and emission patterns of very slow auto-oxidation reactions and much faster processes of water penetration into cereal products. The enhancement of chemiluminescence by the addition of water appears to involve complex processes with an inhomogenous spatial and energy distribution within cereal products. The effect of antioxidants, free radical promoters and scavengers suggests that oxidative radical reactions contribute significantly to the observed chemiluminescence. The possible involvement of hydrophilic interactions, H2O-biopolymers and recombination of trapped radicals is also discussed.

Biopolymers↗
PubMed1999

Bioactive antinutritional peptides derived from cereal prolamins: a review.

Alcohol-soluble endosperm proteins (prolamins) from some cereals (e.g. wheat, barley, and rye) give origin upon proteolytic digestion to biologically-active antinutritional peptides able to adversely affect in vivo the intestinal mucosa of coeliac patients, whereas prolamins from other cereals (e.g. maize and rice) do not. These antinutritional peptides are also able to: (a) prevent in vitro recovery of atrophic coeliac mucosa; (b) to inhibit differentiation of isolated rat fetal and chick fetal intestines; and (c) to interact with undifferentiated cells either agglutinating them or affecting their proliferation and metabolism. Studies performed with A-gliadin, a highly purified bread wheat prolamin fraction, and its fragments obtained either by chemical cleavage of A-gliadin or by synthesis from aminoacids, clearly pointed out to a few small sequences very rich in glutamine and proline residues as the biologically-active agents. Several protective substances, including mannan and N,N',N"-triacetylchitotriose, have been identified as being able to prevent the effects of these peptides in vitro, but the evidence of their in vivo activity is still missing. The present paper provides a synthetic overview of the available data concerning this highly complex matter and offers a critical appraisal of present hypotheses on the action mechanism of biologically-active peptides derived from cereal prolamins.

Amino Acid Sequence↗
PubMed1983

Mycotoxins in cereal grain. Part IX. Zearalenone and Fusaria in wheat, barley, rye and corn kernels.

Toxigenic Fusaria, producing zearalenone, appeared in 31% of cereal grain samples. However zearalenone was present only in 0.5% of 584 cereal samples assayed during 1979-1981 at level 0.2-1.2 mg/kg. Fungi able to form zearalenone were present mainly in grain collected from breeding stations. Fusarium culmorum was dominant among toxigenic isolates from domestic cereals and yield of zearalenone was up to 700 mg/kg.

Animal Feed↗
PubMed2005

Development of a perfusion reversed-phase HPLC method for the separation of soybean and cereal (wheat, corn, and rice) proteins in binary mixtures. Application to the detection of soybean proteins in commercial bakery products.

A perfusion reversed-phase HPLC method enabling the simultaneous separation of soybean and cereal (wheat, corn, and rice) proteins in commercial bakery products has been proposed for the first time. The method utilises an acetonitrile-water gradient containing an ion-pairing agent. Different ion-pairing agents were tried, 0.3% (v/v) acetic acid being observed to enable the separation of soybean from wheat, rice, and corn proteins while with 0.1% (v/v) trifluoroacetic acid only the separation of soybean and corn proteins was possible. Optimisation of the solubilisation conditions for proteins was achieved by testing different acetonitrile concentrations for the simultaneous extraction of soybean and cereal proteins: best recoveries were found with 25% (v/v) acetonitrile + 0.3% (v/v) acetic acid and with 40% (v/v) acetonitrile + 0.1% (v/v) trifluoroacetic acid. Chromatographic conditions such as gradient, temperature, and wavelength detection were also optimised. The method enabled the separation of soybean and cereal proteins in binary mixtures (soybean and wheat, soybean and corn, or soybean and rice proteins) in less than 5 minutes in a total analysis time of 20 min.

Bread↗
PubMed1993

Key features of cereal genome organization as revealed by the use of cytosine methylation-sensitive restriction endonucleases.

Unlike mammalian genomes, cereal (Gramineae) genomes exhibit little suppression of CpG dinucleotides. In cereal genomes, however, most of the numerous potential recognition sites for CpG methylation-sensitive restriction enzymes are methylated. Analysis of cereal genomic libraries and of regions flanking genes indicates that unmethylated NotI sites are useful landmarks for regions containing genes/single-copy sequences. Studies of a rye chromosome arm indicate that its pericentromeric region has a reduced density of unmethylated NotI (and MluI) sites and therefore of genes. Unmethylated MluI and NruI sites are distributed nonrandomly in the genomes of wheat, barley, and rice. Analysis of the genomic blocks defined by these sites in wheat and barley indicates that they are most likely to have arisen by amplification. These observations form the basis of a proposed model for the organization and evolution of the wheat, barley, and rice genomes.

Bacterial Proteins↗
PubMed1977

A possible relationship between high-extraction cereal and rickets and osteomalacia.

The evidence of the present study suggests a strong and possibly causal relationship between high-extraction cereal and rickets and osteomalacia. It seems likely that this occurs when vitamin-D status is border-line. The rachitogenic component of high-extraction cereal remains to be identified; dietary phytate now seems unlikely and phytate-derived polyphosphate esters or dietary phosphorus may be incriminated. The present study strongly indicates that Mellanby's original suggestion of an "anti-calcifying substance" in high-extraction cereal remains valid.

Adult↗
PubMed1984

Effects of an increased content of cereal fibre in the diet of Type 2 (non-insulin-dependent) diabetic patients.

The metabolic effects of an increased dietary content of cereal fibre were studied in 14 Type 2 diabetic subjects. They were given two isoenergetic diets in randomised order during two consecutive 3-week periods. A diabetic diet, containing 18.9 g dietary fibre/6.7 MJ (1600 kcal), was compared with a diet of identical composition except for an increased content of cereal fibre (42.4 g dietary fibre/6.7 MJ). The mean blood glucose level and the urinary glucose excretion were significantly lower in patients on the cereal-fibre-rich diet, while the serum insulin concentrations were similar. The mean blood glucose level was significantly reduced at 0700 h by 6% (p less than 0.05) and at 1100 h by 13% (p less than 0.01) on the high-fibre diet. Consequently the insulin/glucose ratio was higher (33%, p less than 0.02) in patients on the fibre-enriched diet. There were only minor differences with regard to the serum lipoprotein concentrations. The lipoprotein lipase activities were similar in the two dietary groups. The reduction of blood glucose concentrations together with unchanged serum insulin concentrations is compatible with improved peripheral insulin sensitivity.

Aged↗
PubMed1990

A survey of the natural occurrence of Fusarium mycotoxins, deoxynivalenol, nivalenol and zearalenone, in cereals harvested in the Netherlands.

A survey for the natural occurrence of Fusarium mycotoxins, deoxynivalenol (DON), nivalenol (NIV) and zearalenone (ZEN), in Dutch cereals (totaling 29 samples) harvested in 1984/1985, showed that 90%, 79% and 62% of samples were contaminated with DON, NIV and ZEN, respectively. Average contents (ng/g) in the total of positive samples were 221 (DON), 123 (NIV) and 61 (ZEN). Among the cereals examined, the highest concentrations (ng/g) was 3198 (DON), 1875 (NIV) and 677 (ZEN) in a yellow corn sample for animal feed. The results of this survey show that Dutch cereals were relatively significantly contaminated with Fusarium mycotoxins.

Edible Grain↗
PubMed1994

In vitro availability of iron from cereal meal with the addition of protein isolates and fenugreek leaves (Trigonella foenum-graecum).

In vitro iron availability was determined from a standard cereal meal (C) with and without the addition of protein isolates from bovine milk (BP), groundnut milk (GP) and soybean milk (SP). A second set of studies were done to investigate the potentially available iron from fenugreek leaves per se and the effect of their addition on the available iron from the cereal meal. The potentially available iron from the meal increased from 4.91% to 6.73% on the addition of BP. The next best enhancer was GP (4.91% to 6.17%). Although the addition of SP increased the total iron content of the cereal meal significantly, the percent available iron decreased compared to the other milk proteins. The addition of fenugreek leaves (100 g/meal) to the meal increased the total iron content of the meal significantly (3.24 mg to 9.12 mg) (p < .001), however, the available iron was observed to decrease (4.91% vs 4.34%).

Biological Availability↗
PubMed1986

[Cadmium contents in cereal samples in the past--a comparison with the present].

Samples of wheat, rye and barley from previous harvests, some of them dating back even to the past century and placed at the authors' disposal by a number of seed growers and governmental research institutions, were examined for their cadmium content by AAS or ICP-AES. Although no statistically valid data could be obtained owing to the low number of samples, it should nevertheless be stressed that the cadmium content found in cereal samples from harvests of the past were within the range of present-day wheat samples. Thus, the variation in wheat samples from former harvests was 20-100 micrograms/kg which is coincident with an average cadmium content of 50-60 micrograms/kg measured in 2000 what samples from the harvests of the last 10 years as determined in a special survey by the Federal Ministry of Food, Agriculture and Forestry. Comparison of the results for cereals samples from harvests of the past and the samples of the survey conducted by the Ministry does not indicate a trend towards a continuously rising cadmium contents of bread cereals.

Cadmium↗
PubMed1993

[Determination of the folic acid content of grain, cereal products, baked goods and legumes using high-performance liquid chromatography (HPLC)].

The folic acid content of grain, cereal products (including beer), bakery products and legumes was determined by means of high-performance liquid chromatography (HPLC). Free folate (monoglutamate forms) and total folate (monoglutamate + polyglutamate forms) were differentiated. Of the grain analysed, rye, with a mean value of 143 micrograms/100 g, contained more total folate than wheat (mean = 91 micrograms/100 g). The total folate content of bakery products ranged from 14 micrograms/100 g (whole grain rye bread) to 88 micrograms/100 g (crispbread). Beer had a very low total folate content (mean = 3 micrograms/100 ml). The mean of the free folate portion was 76.5% in grain and cereal products and 65.6% in bakery products. Of the legumes analysed, beans (mean = 128 micrograms/100 g) had the highest content of total folate, followed by lentils (103 micrograms/100 g) and peas (57 micrograms/100 g). The mean value of the free folate portion in legumes (73.1%) was comparable with the values of grain, cereal products and bakery products. In addition to tetrahydrofolic acid (THF), 5-methyl-THF and 5-formyl-THF, pteroylglutamic acid (PteGlu) and 10-formyl-PteGlu were determined in all products (except beer). Their proportion (PteGlu + 10-formyl-PteGlu) of the total folate content ranged from 23.5% to 44.4%.

Beer↗
PubMed1994

[Effects of doses of cereal foods and zinc on different blood parameters in performing athletes].

A total of 51 athletes, randomly divided into two groups (with or without zinc supplementation) and respective two subgroups (with or without cereal diet) had been tested for their serum levels in respect of zinc, iron, copper, phosphorus and potassium over a period of 8 weeks, i.e. during a period of competition. Furthermore, subpopulations of lymphocytes were defined. Modifications of the immunologic defense mechanism with special regard to the zinc level could, however, not be detected. Minerals, i.e. zinc and iron revealed to decrease significantly during simultaneous uptake of certain amounts of the cereal products. This is due to the relatively high amounts of phytates in cereals which are capable to bind the trace elements in form of complex salts.

Absorption↗
PubMed1985

[Gas chromatographic study of deoxynivalenol and nivalenol in cereals following derivatization with heptafluorobutyrates].

A method for the analysis of deoxynivalenol (DON) und nivalenol (NV) in cereals is described. This method is suitable for routine use for control purposes. The extract is purified on a florisil column. Derivatization with heptafluorobutyrylimidazole is followed by quantitative determination by means of capillary gas chromatography and electron capture detection (ECD). For verification purposes two-dimensional thin layer chromatography is applied to gc-positive extracts. 67 cereal samples and cereal products from the Berlin market place have been assayed. Depending on the type of sample the limit of detection was 20 to 30 micrograms/kg for DON, and 100 to 300 micrograms/kg for NV.

Chemical Phenomena↗
PubMed1997

Effect of processing on dietary fiber content of cereals and pulses.

Total dietary fiber (TDF), insoluble dietary fiber (IDF) and soluble dietary fiber (SDF) content of rice, wheat, sorghum, maize, ragi, bajra, whole grains of pigeonpea, chickpea, green gram and lentil as well as their dehusked split dhals were analyzed. Cereals except rice flours were made into chapati (unleavened bread), while rice and dhals were cooked in a pressure cooker. After the processing, IDF and SDF contents of these foods were also analyzed. Among the cereals, rice had the lowest TDF (4.1%) and wheat had the highest (12.5%). TDF content of whole pulses ranged from 15.8% in lentil to 28.3% in chickpea. IDF as % of TDF constituted 85 to 89% in whole pulses. Dehusking of pulses into dhals decreased TDF and IDF contents significantly. Among the dhals, green gram dhal had the lowest (8.2%, 6.5%) and chickpea dhal (15.3%, 12.7%) had the highest TDF and IDF contents, respectively. Processing of cereals had no effect on their TDF and IDF contents, with the exception of ragi, where a significant increase in TDF and IDF was observed. Cooking of dhals brought about a significant increase in their TDF and IDF contents.

Dietary Fiber↗
PubMed1997

Isolation and characterization of a 20 kD prolamin from kodo millet (Paspalum scrobiculatum) (L.): homology with other millets and cereals.

The homologus 20 kD prolamin from kodo millet and other minor millets viz. barnyard, little and foxtail millets, were purified using preparative gel electrophoresis and reversed phase high performance liquid chromatography (RP-HPLC). The amino acid composition of the purified 20 kD prolamin protein from different minor millets revealed higher content of glutamic acid, alanine, leucine and serine and lower quantity of lysine and methionine. They contain 55 to 58 percent of non-polar amino acids which make them more hydrophobic than other protein fractions. The total number of amino acid residues per polypeptide chain ranged from 152 to 155 based on theoretical calculation. Peptide mapping of the 20 kD prolamin hydrolyzed with trypsin gave fewer cleavage products than expected. The antigenic relationships among these minor millets and cereals viz. wheat, maize, rice, sorghum, finger millet and pearl millet were studied using the antibody raised against the 20 kD prolamin. Cross reactivity was seen in all the minor millets at the 20 kD region. But in barnyard and little millets lower molecular weight polypeptides also cross reacted with the antibody. Immunoblotting studies revealed that the prolamins from other cereals and millets are related to the 20 kD prolamin of kodo millet. Rice was the only common cereal that did not cross react immunologically with the antibody raised against 20 kD prolamin of kodo millet.

Amino Acids↗
PubMed1996

A cereal centromeric sequence.

We report the identification of a family of sequences located by in situ hybridisation to the centromeres of all the Triticeae chromosomes studied, including the supernumerary and midget chromosomes, the centromeres of all maize chromosomes and the heterochromatic regions of rice chromosomes. This family of sequences (CCS1), together with the cereal genome alignments, will allow the evolution of the cereal centromeres and their sites to be studied. The family of sequences also shows homology to the CENP-B box. The centromeres of the cereal species and the proteins that interact with them can now be characterised.

Autoantigens↗