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Longitudinal changes in the relationship between body mass index and percent body fat in pregnancy.

OBJECTIVE: To determine the longitudinal relationship between body mass index (BMI) and percent body fat in women before and during pregnancy. METHODS: Twenty-seven healthy, nonobese women were evaluated before conception, in early gestation (12-17 weeks), and in late gestation (33-36 weeks). Height and weight were measured and BMI was calculated. Percent body fat was estimated using hydrodensitometry with correction for residual lung volume. RESULTS: The correlation between BMI and percent body fat before conception was r = 0.693 (P < .005); in early gestation it was r = 0.723 (P < .005) and in late gestation r = 0.633 (P < .005). The mean pregravid BMI was 21.54 and the 95% predictive confidence interval (CI) for percent body fat was 18.2, 26.5%. For the mean BMI of 22.26 in early gestation, the predictive 95% CI for percent body fat was 20.0, 29.0%. In late gestation, the mean BMI was 26.04 with a predictive 95% CI for percent body fat 22.5, 30.8%. CONCLUSION: In nonobese women the correlation between BMI and percent body fat remains significant during pregnancy, although the 95% CI for predicting percent body fat from the mean BMI ranges widely.

Adipose Tissue↗

Effect of the amount of body condition loss from the dry to near calving periods on the subsequent body condition change, occurrence of postpartum diseases, metabolic parameters and reproductive performance in Holstein dairy cows.

We investigated the effect of body condition loss from the dry to near calving periods on the subsequent body condition change, the occurrence of postpartum diseases, the serum metabolic parameters total cholesterol, triglyceride, glucose, and urea nitrogen and the number of days to first breeding after calving in Holstein dairy cows. Body condition scoring (using a 5-point scale with quarter-point divisions) was performed on 67 pregnant Holstein dairy cows. Cows were scored once for body condition during the dry period (prepartum day 35 +/- 16), near calving (postpartum day 5 +/- 4), and subsequently at months 1 (+/- day 4), 2 (+/- day 5), 3 (+/- day 5), and 4 (+/- day 4) of lactation. At the same time, blood samples were collected to evaluate serum total cholesterol, triglyceride, glucose and urea nitrogen concentrations. Cows were categorized based on body condition loss from the dry to near calving periods into two groups: the moderate condition loss group (0-0.75 points, n=41), or the marked condition loss group (1.0-1.5 points, n=26). Regular reproductive health examination was conducted by the same investigator once a week. The marked condition loss group lost more body condition (P<0.01) than the moderate condition loss group consistently until month 1 of lactation. The recovery of body condition in the marked condition loss group was more delayed (P<0.01) than in the moderate condition loss group from months 1 to 4 of lactation. The occurrence of metritis and metabolic diseases (including abomasal displacement, milk fever, ketosis) was greater (P<0.01) in the marked condition loss group (62 and 23%) than in the moderate condition loss group (27 and 2%), respectively. The total cholesterol concentration was lower (P<0.05) in the marked condition loss group (167 +/- 5.3 mg/dl) than in the moderate condition loss group (183 +/- 5.8 mg/dl) at month 1 of lactation, however, the cholesterol levels at the other periods were not different (P>0.05) between the two groups. The triglyceride, glucose, and urea nitrogen concentrations were not different (P>0.05) from the dry period to month 4 of lactation between the two groups. The number of days to first breeding after calving was longer (P<0.05) in the marked condition loss group (103 +/- 7.8 day) than in the moderate condition loss group (87 +/- 5.3 day). We conclude that marked body condition loss from the dry to near calving periods results in the increased occurrence of postpartum metabolic and reproductive diseases, decreased serum total cholesterol concentrations at month 1 of lactation and a longer interval to first breeding after calving in Holstein dairy cows, probably due to the more severe energy deficit reflected by unfavorable body condition score change during early lactation.

Animals↗

The effect of conjugated linoleic acid supplementation after weight loss on body weight regain, body composition, and resting metabolic rate in overweight subjects.

OBJECTIVE: To study the effects of 13 weeks conjugated linoleic acid (CLA) supplementation in overweight subjects after weight loss on weight regain, body composition, resting metabolic rate, substrate oxidation, and blood plasma parameters. DESIGN: This study had a double-blind, placebo-controlled randomized design. Subjects were first submitted to a very-low-calorie diet (VLCD 2.1 MJ/d) for 3 weeks after which they started with the 13-week intervention period. They either received 1.8 g CLA or placebo per day (low dosage, LD) or 3.6 g CLA or placebo per day (high dosage, HD). SUBJECTS: A total of 26 men and 28 women (age 37.8+/-7.7 y; body mass index (BMI) 27.8+/-1.5 kg/m(2)). MEASUREMENTS: Before VLCD (t=-3), after VLCD but before CLA or placebo intervention (t=0) and after 13-week CLA or placebo intervention (t=13), body weight, body composition (hydrodensitometry and deuterium dilution), resting metabolic rate, substrate oxidation, physical activity, and blood plasma parameters (glucose, insulin, triacylglycerol, free fatty acids, glycerol and beta-hydroxy butyrate) were measured. RESULTS: The VLCD significantly lowered body weight (6.9+/-1.7%), %body fat, fat mass, fat-free mass, resting metabolic rate, respiratory quotient and plasma glucose, insulin, and triacylglycerol concentrations, while free fatty acids, glycerol and beta-hydroxy butyrate concentrations were increased. Multiple regression analysis showed that at the end of the 13-week intervention, CLA did not affect %body weight regain (CLA LD 47.9+/-88.2%, CLA HD 27.4+/-29.8%, Placebo LD 32.0+/-42.8%, Placebo HD 22.5+/-37.9%). The regain of fat-free mass was increased by CLA (LD 6.2+/-3.9, HD 4.6+/-2.4%) compared to placebo (LD 2.8+/-3.2%, HD 3.4+/-3.6%), independent of %body weight regain and physical activity. As a consequence of an increased regain of fat-free mass by CLA, resting metabolic rate was increased by CLA (LD 12.0+/-11.4%, HD 13.7+/-14.4%) compared to placebo (LD 9.1+/-11.0%, HD 8.6+/-8.5%). Substrate oxidation and blood plasma parameters were not affected by CLA. CONCLUSION: In conclusion, the regain of fat-free mass was favorably, dose-independently affected by a 13-week consumption of 1.8 or 3.6 g CLA/day and consequently increased the resting metabolic rate. However, it did not result in improved body weight maintenance after weight loss.

Adult↗

Body image or body images?: Comparative, multidimensional assessment among college students.

A major criticism of research on body image concerns the dubious assumptions of the unidimensionality of the construct and the equivalence of body-image measures. Our study of 125 male and female college students compared multiple measurement methods within each of two modalities of body image: (a) perceptual (body-size estimation) and (b) attitudinal (body-image affect and cognition). Clinically relevant indices of psychological adjustment and eating disturbance were included. Results clearly supported the distinction between attitudinal and perceptual modalities of body image and indicated more convergent and discriminant validity for the former than for the latter. Whereas one method of assessing size-estimation accuracy and most body-image attitudes produced relationships with maladjustment, only attitudinal body image and perceptual, self-ideal discrepancy measures were significantly linked to eating disturbance. Relationships were generally more consistent for women than for men. Implications are discussed in the context of extant and future research on body image with clinical and nonclinical populations.

Adaptation, Psychological↗

The use of comparative 137Cs body burden estimates from environmental data/models and whole body counting to evaluate diet models for the ingestion pathway.

Rongelap and Utirik Atolls were contaminated on 1 March 1954, by a U.S. nuclear test at Bikini Atoll code named BRAVO. The people at both atolls were removed from their atolls in the first few days after the detonation and were returned to their atolls at different times. Detailed studies have been carried out over the years by Lawrence Livermore National Laboratory (LLNL) to determine the radiological conditions at the atolls and estimate the doses to the populations. The contribution of each exposure pathway and radionuclide have been evaluated. All dose assessments show that the major potential contribution to the estimated dose is 137Cs uptake via the terrestrial food chain. Brookhaven National Laboratory (BNL) has carried out an extensive whole body counting program at both atolls over several years to directly measure the 137Cs body burden. Here we compare the estimates of the body burdens from the LLNL environmental method with body burdens measured by the BNL whole body counting method. The combination of the results from both methods is used to evaluate proposed diet models to establish more realistic dose assessments. Very good agreement is achieved between the two methods with a diet model that includes both local and imported foods. Other diet models greatly overestimate the body burdens (i.e., dose) observed by whole body counting. The upper 95% confidence limit of interindividual variability around the population mean value based on the environmental method is similar to that calculated from direct measurement by whole body counting. Moreover, the uncertainty in the population mean value based on the environmental method is in very good agreement with the whole body counting data. This provides additional confidence in extrapolating the estimated doses calculated by the environmental method to other islands and atolls.

Body Burden↗

The relation between carotid body chemoreceptor discharge, carotid sinus pressure and carotid body venous flow.

1. Activity in forty-two single chemoreceptor afferent fibres from the carotid body in thirty-nine cats was measured when the carotid body was naturally and artificially perfused. In nine of these cats, carotid body venous flow was also measured.2. When pressure within the carotid sinus segment was suddenly raised or lowered, chemoreceptor activity changed in the opposite direction within the first 5-10 sec by an amount which was significantly greater than the variation of activity in the control period. Thereafter activity stabilized at a level which was not different from control.3. Whether the carotid body was naturally or artificially perfused, carotid body chemoreceptor activity, following this initial transient change, was unaffected in eight out of twelve fibres by alterations in carotid sinus pressure within the range 60-160 mm Hg and carotid body venous flow 10-60 mul./min, blood gas tensions and pH being maintained constant. In the four remaining fibres, chemoreceptor activity increased slightly but significantly as pressure was lowered in this range. Chemoreceptor activity increased in all fibres tested when pressure was lowered below 50-60 mm Hg.4. Chemoreceptor response curves to changes in P(a,O2) (30-450 mm Hg), P(a,CO2) (27-62 mm Hg) or [H(+)](a) (3-7 x 10(-5) m-equiv/l.) were not significantly different whether the carotid body was perfused (a) naturally at the prevalent systemic pressure, (b) artificially at the same pressure, or (c) artificially at one higher pressure, 130 or 140 mm Hg.5. These results indicate that the carotid body chemoreceptors are relatively unaffected by sustained changes in arterial pressure or in total carotid body flow within the physiological range.

Animals↗

Dietary protein adequacy and lower body versus whole body resistive training in older humans.

This study assessed the effects of long-term consumption of the United States Recommended Dietary Allowance (RDA) for protein by older people who were sedentary or performed resistive training (RT) on body composition, skeletal muscle size and protein metabolism, and if the number of muscle groups trained influenced the muscle hypertrophy response to RT. Twelve men and 17 women (age range 54-78 years) completed this 14 week controlled diet and exercise study. Throughout the study, each subject completely consumed daily euenergetic menus that provided the RDA of 0.8 g protein kg(-1) day(-1). From study weeks 3-14 (weeks RT1-RT12), 10 subjects (four men, six women) performed whole body RT (WBRT), nine subjects (four men, five women) performed lower body RT (LBRT) and 10 subjects (four men, six women) remained sedentary (SED). Both the LBRT and WBRT groups performed knee extension and flexion exercises, and the WBRT group also performed chest press and arm pull exercises (three sets per exercise at 80 % of one repetition maximum, 3 days per week for 12 weeks). From week 2 (baseline) to week RT12, muscle strength increased in muscle groups trained in the LBRT and WBRT groups, and was not changed in the SED group. From baseline to week RT12, whole body muscle mass and protein-mineral mass were not changed, fat-free mass (P = 0.004) and total body water (P = 0.013) were decreased, and percentage body fat was increased (P = 0.011) in these weight-stable older people, independent of group assignment. The RT-induced increases in mid-thigh muscle area (from computed tomography scans) were comparable in the LBRT and WBRT groups (2.13 +/- 1.26 cm(2) and 2.17 +/- 1.24 cm(2), respectively), and were different from those in the SED group, which lost muscle area (-1.74 +/- 0.57 cm(2); group-by-time P < 0.05). From baseline to week RT12, 24 h urinary total nitrogen excretion decreased (P < 0.001), nitrogen balance shifted from near equilibrium to positive, whole body leucine oxidation (from the infusion of L-[(13)C]leucine) decreased (P < 0.05) and net (postabsorptive vs. postprandial) leucine balance (P < 0.05) increased from near equilibrium to positive, with no differences in responses over time among the three groups. In conclusion, the number of muscle groups trained did not influence whole body protein metabolism or RT-induced muscle hypertrophy in older people. Most of these data are consistent with a successful adaptation to the RDA for protein. However, research should continue to question whether the decreases in fat-free mass and total body water observed in all subjects, and the decrease in mid-thigh muscle area in the SED group, are physiological accommodations, and whether the RDA for protein might be marginally inadequate for older people to maintain skeletal muscle.

Aged↗

Comparison of different body size parameters for individual dose adaptation in body CT of adults.

PURPOSE: To investigate prospectively which of several body size parameters are suitable for individual dose adaptation in body computed tomography (CT) of adults. MATERIALS AND METHODS: Three body regions (thorax, abdomen, pelvis) were scanned exclusively for clinical reasons, with institutional ethical approval and informed consent. For each of the three regions, 50 men and 50 women (aged 18-87 years) were studied (300 scans total). Individual x-ray properties for each scan were summarized with a water-equivalent diameter (Dw). Different body size parameters, based on weight, height, and shape, were correlated with Dw by using regression analysis. This resulted in Dw estimation errors of different magnitudes, indicated with 95% prediction intervals. The errors from weight were compared with those from each of the other body parameters by using comparison of variance in paired samples (P < .05). In addition, a topogram-based estimate for Dw was studied, which simulated an automated body size measurement. RESULTS: For the thorax, abdomen, and pelvis, mean Dw was 28.0, 29.1, and 29.3 cm, and estimation of Dw from weight enabled 95% prediction intervals of +/-2.5, +/-2.4, and +/-2.6 cm, respectively. Combinations of height and weight were only slightly more or even less exact than were measurements from only weight. Diameter-related parameters such as body circumference were similar to or better than weight. However, the topogram-based estimate was significantly more exact. CONCLUSION: Body weight and circumference enable suitable estimates for individual dose adaptation in body CT of adults if automated dose adaptation is not available.

Adolescent↗

Density of fat-free body mass: relationship with race, age, and level of body fatness.

The two-compartment body composition method assumes that fat-free body mass (FFM) has a density of 1.100 kg/l. This study tested the hypothesis that FFM density is independent of race, age, and body fatness. Subjects were 703 black and white subjects, ages 20-94 yr, with body mass index (BMI) 17-35 kg/m2. Body composition was assessed using a four-compartment model based on tritium dilution volume, body density by underwater weighing, bone mineral by dual-energy X-ray absorptiometry, and body weight. No relationship was observed between FFM density and race or BMI. A tendency was observed for a lower FFM density only in older white women. The difference in percent body fat (delta fat) between the four-compartment model and underwater weighing was < 2% for all groups. Race, age, and BMI explained only 2.3 (women) and 1.4% (men) of the variance in delta fat, whereas the total body water fraction of FFM explained 77%. In contrast to current thinking, these results show that the assumption of constant FFM density is valid in black, elderly, and obese subjects.

Adipose Tissue↗

C bodies and R bodies in the epithelial cells of normal and diseased human rectum.

Small spherical, extracellular bodies (C bodies) measuring 20 to 80 nm in diameter and enclosed by a trilaminar membrane 7 nm wide surrounded the microvilli of human rectal epithelial cells. Similar bodies were observed within membrane-bound vesicles (R bodies) in the apical cytoplasm. The C bodies associated with the mature epithelial cells of the luminal surface were more numerous than those associated with the immature cells of the crypts, where the R bodies predominated. A decreased number of C bodies was observed in ulcerative colitis. We concluded that the membrane-bound C bodies surrounding the microvilli were cellular in origin and arose from R bodies within the cytoplasm of the rectal epithelial cells.

Cell Membrane↗

Predicting body weight from body measurements in Asian elephants (Elephas maximus).

Accurate estimates of body weight can be useful in the evaluations of feeding programs, nutritional status and general health, and in calculation of dose levels (such as for anesthesia)-thus providing a valuable tool for captive elephant management. We used body measurements of 75 Asian elephants (Elephas maximus) to predict body weight. Weight, heart girth, height at the withers, body length, and foot-pad circumference were measured. All possible linear regressions of weight on one, two, three, or four body measurements were calculated. The highest correlation with a single measurement was that between heart girth and weight (R2 = 0.90). The data were also divided into age groups (1-13, 18-28, 29-39, and 40-57 yr), and all possible linear regressions were calculated for each group (there were no elephants aged 14-17 yr). Adding body length or pad circumference to heart girth resulted in a slight increase in R2. We conclude that body weight in Asian elephants can be predicted from body measurements and that heart girth is the best predictor. A second body measurement might improve predictive accuracy for some age groups.

Age Factors↗

Tissue-specific lipoprotein lipase: relationships to body composition and body fat distribution in normal weight humans.

Twenty-six normal weight subjects (22 female, 4 male) were studied to determine the relationships of fasting levels of lipoprotein lipase in gluteal adipose tissue (ATLPL) and skeletal muscle (SMLPL) to body composition and body fat distribution. No relationship was found between fasting gluteal ATLPL and percent (%) body fat. There was, however, an inverse relationship between fasting SMLPL (from the vastus lateralis) and %body fat (p=0.005). A strong inverse correlation was also seen between fasting ATLPL and waist/hip ratio (p=0.0006), a measurement of body fat distribution. These relationships existed with or without the male subjects included. The tissue-specific relationships of lipoprotein lipase to body composition and body fat distribution could relate to the development of obesity or the maintenance of normal body weight by the effects of the lipase on the partitioning of lipoprotein triglyceride fatty acids.

Adipose Tissue↗

Body composition by the four-compartment model: validity of the BOD POD for assessing body fat in Mexican elderly.

OBJECTIVE: The aims of this study were to validate BOD POD in a wide sample of healthy and independent Mexican elderly men and women subjects using the 4 compartment (4C) model as the reference method, and to evaluate the assumptions of the densitometric two-compartment (2C) model. DESIGN: Cross-sectional study designed to assess body composition and validation of a method based on 2C model (BOD POD). SETTING: Urban and rural regions of Sonora, Mexico. SUBJECTS: Two hundred and two free-living subjects >or=60 years old were completed in this study. METHODS: Body density and body fat were measured by the BOD POD, total body water by deuterium dilution and total body bone ash by dual energy X-ray absorptiometry. Body composition was determined using Baumgartner's equation. RESULTS: Percent body fat by the 4C model was 31.2 and 42.5% in men and women, respectively (P<0.001). Group mean accuracy of body fat by BOD POD against that of the 4C model showed an effect of sex (P<0.001), but not the method (P=0.27). Results of individual accuracy showed no significant difference with the identity line and the slope was significantly different from zero or a slope similar to one. Precision assessed by model R (2) was high for all subjects and for men and women by separate. The standard error of the estimate was low for all and for men and women by separate. Bland and Altman analysis showed no significant bias. CONCLUSION: The BOD POD technique is a valid and reliable method compared to the 4C model and it could be applied in subjects with similar physical and anthropometric characteristics to subjects of this study.

Absorptiometry, Photon↗

Estimation of minimal wrestling weight using measures of body build and body composition.

This study evaluated field methods for establishing minimal body weight in wrestlers based upon estimates of fat-free body weight (FFB): 239 high school wrestlers were measured for height, weight, 13 diameters, 24 circumferences, and 7 skinfolds, as well as for underwater weight to determine body density and resultant FFB. To estimate FFB, anthropometric models were used which were based upon diameters, circumferences, and/or skinfolds. Correlations between actual and predicted FFB were high, ranging from r = 0.89 to 0.92 for the models employing diameters and r = 0.96 for those employing circumferences, which respectively increased to r = 0.96 and r = 0.97 when body weight was included in theses models. The skinfold-based and mixed model estimates of FFB were r = 0.97 and r = 0.91 to 0.97, respectively. The corresponding total error (TE) in these estimates for the diameter-based models ranged from 6.54 to 3.80 kg (inclusion of body weight further reduced TE to 2.77 kg). Among the circumference models, TE ranged from 2.50 to 2.33 kg, with inclusion of body weight accounting for the lower value. Skinfold-based estimates of FFB demonstrated a TE level ranging from 2.48 to 2.33 kg. The lowest TE (2.32 kg) was demonstrated by a mixed model which included skinfolds, diameter, height, and body weight. Cross-validation of these new equations revealed the lowest TE values (less than 2.2 kg) for the skinfold and the mixed model equations, thereby identifying highly valid field techniques for determining the present level of FFB, which can serve as the basis upon which minimal body weight estimates can be made.

Adipose Tissue↗

Validation of a 3-dimensional photonic scanner for the measurement of body volumes, dimensions, and percentage body fat.

BACKGROUND: The 3-dimensional photonic scan (3DPS) technique has been used during the past decade in the fashion industry and for epidemiologic surveys to estimate human body sizes. OBJECTIVE: The objective of the study was to validate the accuracy of a recently developed 3DPS (C9036-02; Hamamatsu Photonics KK, Hamamatsu, Japan) for the measurement of body volume, circumferences, lengths, and percentage body fat with the use of underwater weighing (UWW) and tape measures as criterion methods. DESIGN: Ninety-two subjects (44 females and 48 males) aged 6-83 y and weighing 23-182 kg (52-400 lbs) participated in the study. The subjects were measured while they wore minimal clothing and a head cap. Similar measurements were performed on a mannequin with and without clothing RESULTS: All subjects were measured with 3DPS and a tape measure; 63 subjects underwent UWW and residual lung volume measurements. The values obtained with 3DPS were slightly but significantly greater than those obtained with UWW for body volume (81.9 +/- 4.0 L compared with 81.5 +/- 4.0 L, P < 0.0001) and those obtained with a tape measure for circumferences (P < 0.001), but the values for percentage body fat were not significantly different between 3DPS and UWW (P = 0.648). The values obtained with 3DPS were significantly greater than those obtained by UWW and a tape measure for the clothed mannequin, but the values were not uniformly significantly different for the mannequin without clothing. CONCLUSIONS: The 3DPS measures body volume, circumferences, and length rapidly and accurately. However, to generate an accurate total-body volume measurement with 3DPS to estimate percentage body fat, the subjects must wear close-fitting minimal clothing and be able to stand motionless for 10 s (normal scan mode) while holding their breath, which is done immediately after a maximum expiration.

Adipose Tissue↗

Use of bioelectrical impedance analysis to estimate body fluid compartments after acute variations of the body hydration level.

Physiological measurements including body mass, plasma osmolality, natremia, plasma volume measured by Evans Blue dilution, and total body water (TBW) and extracellular water (ECW) volumes estimated by bioelectrical impedance analysis (BIA) were recorded in eight healthy young Caucasian subjects before and after acute variations of their body hydration state on four separate occasions: 1) euhydration or control trial (C); 2) heat-induced dehydration of 2.8% body mass (D); 3) exercise-induced dehydration of 2.8% body mass (E); and 4) glycerol-hyperhydration (H). Heart rate, rectal and mean skin temperatures were also recorded throughout the experiment. The main result of the study is that BIA only half predicted the body water loss after exercise, although conditions were standardized (electrode placement, side of the body, limb position, posture, and ambient temperature). Differences in body temperatures cannot explain such an unexpected result, nor did the study of plasma osmolality and sodium concentration. If BIA appears to adequately predict changes in TBW after heat-induced dehydration and glycerol hyperhydration, further studies including measures of TBW and ECW by dilution tracer methods would be necessary to establish the validity of using the BIA method to measure such changes and to interpret ECW variations.

Adult↗

Body composition by three-compartment model and relative validity of some methods to assess percentage body fat in mexican healthy elderly subjects.

BACKGROUND: In Mexico, there is scarce information about the body composition in the elderly. Some researchers have measured body fat by anthropometry and bioelectrical impedance. These techniques are practical and can be used in the field; however, proper validation is required. This implies the use of accurate and precise methods. In the elderly, Siri's three-compartment (3C) model has been proposed as feasible alternative to the gold standard four-compartment model. OBJECTIVE: To evaluate the body composition by the 3C model and the relative validity of densitometry and hydrometry methods to assess the percentage body fat (%BF) in 37 healthy Mexican elderly subjects. METHODS: The body density was measured by air displacement plethysmography, total body water was evaluated by means of an isotopic dilution technique, and two different hydration factors were used to determine the fat-free mass. These measurements were used to calculate %BF by the 3C model. Accuracy and precision of the methods were tested by a two-way analysis of variance and regression procedures and bias by Bland and Altman analysis. RESULTS: In men and women as a whole group, the %BF by the 3C model was 34.4 +/- 8.01. Women had higher values of %BF as compared with men (41.7 +/- 3.7 vs. 29.4 +/- 6.4; p < 0.0001). The regression procedures showed that air displacement plethysmography was accurate and precise only in women and in the whole group. Regression analyses of %BF by total body water (using both hydration factors) and 3C model showed that the intercepts were not different from zero and that the slopes were not different from 1.0 in men and women separately and as group. CONCLUSION: In this study, the total-body water method revealed a good relative validity (accuracy, precision, and freedom from bias) as compared with the 3C model in men and women both separately and as a group and may be acceptable for the estimation of %BF in individuals or groups of healthy elderly subjects.

Adipose Tissue↗

Creatine monohydrate supplementation on body weight and percent body fat.

Seventeen active males (age 22.9 +/- 4.9 year) participated in a study to examine the effects of creatine monohydrate supplementation on total body weight (TBW), percent body fat, body water content, and caloric intake. The TBW was measured in kilograms, percent body fat by hydrostatic weighing, body water content via bioelectrical impedance, and caloric intake by daily food log. Subjects were paired and assigned to a creatine or placebo group with a double-blind research design. Supplementation was given for 4 weeks (30 g a day for the initial 2 weeks and 15 g a day for the final 2 weeks). Subjects reported 2 days a week for supervised strength training of the lower extremity. Significant increases before and after the study were found in TBW (90.42 +/- 14.74 to 92.12 +/- 15.19 kg) and body water content (53.77 +/- 1.75 to 57.15 +/- 2.01 L) for the creatine group (p = 0.05). No significant changes were found in percent body fat or daily caloric intake in the creatine group. No significant changes were noted for the placebo group. These findings support previous research that creatine supplementation increases TBW. Mean percent body fat and caloric intake was not affected by creatine supplementation. Therefore weight gain in lieu of creatine supplementation may in part be due to water retention.

Adipose Tissue↗