THYMUS GLAND FRACTIONATION AND THYMOCYTE LYSIS.
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The system for the immunomorphological evaluation of the degree of the human and animal thymus transformation is suggested. The degree of the epithelium demasking and the character of lymphocyte emigration were revealed due to the increase of the surface and alteration of the size of epithelial cells which under normal conditions are completely screened by lymphocytes. This was done by means of the fluorescent antibodies to the cells of epithelial reticulum of the thymic lobule cortex and some cells of the medulla layer (prekeratins). This system permits a more circumstantial characteristics of the histological changes in the thymus when the cortical part of the lobule loses the capacity for lymphocyte retention in the epithelial reticulum.
Using 75Se methionine the authors performed a scintigraphic examination of the thymus in 65 patients, 15 of whom were suffering from myasthenia and 48 (control) from various neoplastic pathologies of the thyroid and the larynx. The examination has made it possible to discover parts of the thymus active tissue including those remaining after thymectomy. The method enables one to control the results of radiation and surgical treatment of myasthenic patients.
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