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At least 307 records · Page 17Linked to original sources
PubMed2001

[Determination of sarsasapogenin in "tian dong" by TLCS].

This paper reported the determination of sarsasapogenin in 12 samples from 7 species and varieties of Asparagus genus whose commercial name is "Tian-Dong"(Radix Asparagi), Asparagus cochichinensis(Lour.) Merr, A. cochichinensis (lour.) Merr. var. gaudichaudianus (Kunth) X. D. Luo and G. J. Xu, A. taliensis Wang et Tang, A. munitus Wang et Tang, A. myriacanthus Wang et S. C. Chen, A. meioclados Levl and A. trichoclados (Wang et Tang) Wang et S. C. Chen by TLCS. The results showed that it was no pertinence between content of sarsaspogenin with the species, and the content of sarsasapogenin in the tuberous roots was inverse ratio with the commercial grande in same species.

Aspalathus↗
PubMedDate not supplied

[Use of enzymic preparations during diosgenine isolation from Dioscorea caucasica Lipsky].

The use of enzymic preparations of the cellulolytic and macerating effect was studied as applied to the isolation of diosgenine from rhizomes of Dioscorea caucasica Lypsky. The enzymic treatment of the steroid containing raw material prior to acid hydrolysis increased the yield of diosgenine by 30-48%. It is suggested that additional extraction of diosgenine takes place due to: 1) enzymic hydrolysis of structural polysaccharide components of the plant tissue and intercellular binding materials and 2) disintegration of glycoside bonds of saponins.

Cellulase↗
PubMed1999

[Determination of sarsasapogenin in Anemarrhena asphodeloides Bunge by GC].

OBJECTIVE: To determine sarsasapogenin in Anemarrhena asphodeloides. METHOD: Chloromethane extract (1 microliter) with cholesterol as internal standard was analyzed on HP-1 column, operated at 270 degrees C with N2 as carrier gas and FID. RESULT: The method was linear within the range of 0.245-2.94 micrograms.microliter-1, with a correlation coefficient of 0.9996. The average recovery was 95.52% +/- 1.77%, CONCLUSION: The method is reproducible, rapid and sensitive.

Anemarrhena↗
PubMed2001

[Screening of steroidal saponins from the bulbs of Lilium brownii var. colchesteri by combination of high performance liquid chromatography-electrospray ionization mass spectrometry and electron impact mass spectrometry].

With the combination of high performance liquid chromatography-electrospray ionization mass spectrometry (HPLC/ESI-MS) and electron impact mass spectrometry (EI-MS), two steroidal saponins, one compound containing three glycosyls and tigogenin and the other one containing three glycosyls and diosgenin, from the bulbs of Lilium brownii var. calchesteri in China have been screened. In the method, on-line HPLC/ESI-MS allows us to obtain rapidly useful information about the molecular weight and the glycosyl chain of glycoside without the necessity of isolating individual compounds, but little information about steroidal sapogenins. Just with 1 mg to 2 mg of pure sample, off-line EI-MS allows us to acquire useful information about a steroidal sapogenin of saponins, but it is difficult to obtain the molecular ion peak. The combination of HPLC/ESI-MS and EI-MS is well suitable for rapidly screening steroidal saponins from plants.

Chromatography, High Pressure Liquid↗
PubMed2003

Different contribution of apoptosis to the antiproliferative effects of diosgenin and other plant steroids, hecogenin and tigogenin, on human 1547 osteosarcoma cells.

Regulation of growth arrest and apoptosis are, in part, controlled by the tumor suppressor p53 after its phosphorylation which causes a determinant role in its functional activation. Moreover, PPAR regulate many functions such as proliferation and apoptosis. We compared the biological activity of diosgenin with hecogenin and tigogenin, plant steroids structurally close to diosgenin, on proliferation rate, cell cycle distribution and apoptosis in human 1547 osteosarcoma cells. We found that all three molecules have an antiproliferative effect but gel shift analysis demonstrated that none of the plant steroids transactivated PPAR in human 1547 osteosarcoma cells whereas these molecules induced NF-kappaB binding to DNA. Although these plant steroids have a very close structure, only diosgenin caused a cell cycle arrest associated with strong apoptosis. This biological action seems correlated with a large increase of p53 protein expression. This fact was showed by immunofluorescence analysis which confirmed that diosgenin strongly enhanced the activation of p53 in contrast to hecogenin and tigogenin actions.

Antineoplastic Agents↗
PubMed2003

Evidence for essential histidine and dicarboxylic amino-acid residues in the active site of UDP-glucose : solasodine glucosyltransferase from eggplant leaves.

Effects of several chemical probes selectively modifying various amino-acid residues on the activity of UDP-glucose : solasodine glucosyltransferase from eggplant leaves was studied. It was shown that diethylpyrocarbonate (DEPC), a specific modifier of histidine residues, was strongly inhibitory. However, in the presence of excessive amounts of the enzyme substrates, i.e. either UDP-glucose or solasodine, the inhibitory effect of DEPC was much weaker indicating that histidine (or histidines) are present in the active site of the enzyme. Our results suggest also that unmodified residues of glutamic (or aspartic) acid, lysine, cysteine, tyrosine and tryptophan are necessary for full activity of the enzyme. Reagents modifying serine and arginine residues have no effect on the enzyme activity.

Amino Acids, Dicarboxylic↗
PubMed2006

[Study on steroidal compounds from Dioscorea zingiberensis].

OBJECTIVE: To study the chemical constituents of Dioscorea zingiberensis. METHOD: Manifold chromatography methods were used to separate the chemical constituents, and the chemical stuctures were determined by spectral analyses. RESULT: 6 compounds were isolated from Dioscorea zingiberensis and identified as deltonin, protodeltonin, zingiberensis newsaponin, dioscin, 3-O-[beta-D-glucopyranosyl(1 --> 4)]-beta-D-glucopyranosyl-diosgenin, diosgenin. CONCLUSION: Deltonin and protodeltonin were isolated from this plant for the first time.

Dioscorea↗
PubMed1990

[Steroidal saponins from Asparagus filicinus].

Three new steroidal saponins, aspafilioside A, B and C together with a known saponin 22-methoxy ASP-IV and beta-ecdysone were isolated from the root of Asparagus filicinus Buch.-Ham. (Chinese name as xiao-bai-bu), a folk medicine of the minorities in Yunnan Province, China, used for the treatment of bronchitis, pneumonitis and cough. Their structures were established as sarsasa pogenin-3-O-beta-D-xy lopyranosyl (1----4)-beta-D-glucopyranoside (I), sarsasapogenin-3-O-beta-D-xylopyranosyl (1----4) [alpha-L-arabinopyranosyl (1----6)]-beta-D-glucopyranoside (II) and (25S)-5 beta-furost-3 beta, 22, 26-triol-3-O-beta-D-xylopyranosyl (1----4) [alpha-L-arabinopyranosyl(1----6)]-beta-D-glucopyranoside-26-O-bet a-D- glucopyranoside (III) by spectral and chemical methods.

Drugs, Chinese Herbal↗
PubMed1989

[TLC-densitometric determination of sarsasapogenin in Anemarrhena asphodeloides Bge].

The sarsasapogenin content in Anemarrhena asphodeloides and its preparations was assayed by TLC-densitometric method. The developing solvent used was benzene-acetone (9:1). The chromatographic plate was sprayed with a mixture of 8% vanillin in alcoholic solution-70% H2SO4 solution (0.5:5). This plate was scanned using 445 nm as wavelength lambda s and 600 nm as wavelength lambda s.

Chromatography, Thin Layer↗