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Comparative evaluation of molecular technologies for the identification of prevalent non-tuberculous mycobacteria in pulmonary infections: a systematic review and meta-analysis.

BACKGROUND: The increasing prevalence of non-tuberculous mycobacteria pulmonary disease (NTM PD) is a burden to public health. Successful management of NTM PD critically depends on accurate species identification and reliable drug susceptibility testing to guide appropriate antibiotic therapy. Emerging molecular technologies offer rapid diagnostic solutions compared to conventional methods, but their performance varies. This study aims to provide a comprehensive evaluation of current molecular techniques for NTM identification and to present a global antibiotic resistance profile. METHODS: A systematic literature search was conducted in PubMed and Web of Science for studies published between 2005 and 2024. Studies applying molecular methods for NTM identification and resistance detection in humans were included. Data on study characteristics, diagnostic methods, sample types, sample sizes, identification sensitivity, and drug susceptibility results were extracted. Meta-analysis was performed using R with the meta4diag package. The quality of included studies was assessed using the QUADAS-2 tool. RESULTS: The analysis included 49 studies on NTM identification and 33 studies on antibiotic resistance. For species identification, all evaluated molecular technologies (MALDI-TOF MS, PCR-based methods, Sequencing, DNA chip, and DNA strip) demonstrated high pooled sensitivities (>0.92). Subgroup analysis revealed that sample type significantly affected performance for MALDI-TOF MS. Preliminary analysis of antibiotic resistance rates revealed varying patterns. For slowly growing mycobacteria, a significantly high Ethambutol resistance rate was observed in M. avium (69.20%). Among rapidly growing mycobacteria, resistance to Imipenem was notable (54.22%), and Clarithromycin resistance varied significantly within the Mycobacterium abscessus complex. CONCLUSION: Emerging molecular technologies have revolutionized the methodology for NTM identification with excellent performance. However, their performance can be influenced by sample type, particularly for MALDI-TOF MS. The alarming and heterogeneous antibiotic resistance patterns also highlight the critical need for rapid and accurate species identification and drug susceptibility testing to inform effective therapeutic strategies. Key messagesMolecular technologies demonstrate high accuracy for NTM identification.Antibiotic resistance is a serious concern with variations among NTM species and subspecies.Rapid and accurate species identification and drug susceptibility testing are crucial for guiding effective clinical management of NTM PD.

Humans↗

When teachers go the extra mile: foci of organisational identification as determinants of different forms of organisational citizenship behaviour among schoolteachers.

BACKGROUND: Psychological variables should play an important role in determining teachers' involvement in behaviours not directly or formally forced by contracts. Organisational identification as proposed from the Social Identity Approach is examined as a possible determinant of organisational citizenship behaviour (OCB) among schoolteachers. AIM: The aim of the present study was to explore the relationships between different foci of organisational identification and different forms of OCB in schools. SAMPLE: Data sets of altogether 447 German school teachers who filled in all relevant items in a cross-sectional questionnaire are used for analyses in the present study. METHODS: Standardised questionnaires measuring organisational identification and OCB were administered. RESULTS: Exploratory and confirmatory factor analyses revealed the proposed foci of identification (i.e., career identification, team identification, and organisational identification), as well as different forms of OCB (i.e., OCB towards the own qualification, towards the team, and towards the organisation). Structural equation modelling supports the main hypothesis that foci of identification relate differentially to forms of OCB. CONCLUSIONS: The results emphasise the importance of organisational identification as a determinant of OCB in schools. Practical implications are discussed.

Adult↗

Patient's permanent identification and PMSI chaining system. Complementary or competing methods?

In order to "chain" all the information concerning their patients' files, hospitals have set up their own identification system. This system is not easy to manage and can encounter several difficulties. When entering the hospital, each patient is given an identification number resulting mainly from his ID card, his Health Care card data and other data... The clerk who registers a patient seeks for possible prior hospital stays of this patient: should the patient be unknown, a new record of identification will be created; but should this patient be already registered, the clerk will link the patient's new hospital stay to the already existing file and update the permanent patient's identification data if necessary. In spite of all the care taken to carry out this process, some errors may occur: creation of "doubles", which means creating a new patient's file for an already existing patient, or conversely wrong chaining for an already existing patient. In order to track down these kinds of errors hospitals have set up a quality system. Moreover, since January 2001, in the frame of the Prospective Payment System (in French PMSI), a hospital stay chaining system based on a calculated key (H coding) has been designed. If the use of this key is meant to provide statistics concerning patients' cares and not patients' stays it may also become part of the quality policy of an establishment concerning patients' permanent identification data. Considering the 61 486 hospital stays at the C.H.U of Nancy for the first six months in 2001, it has been possible to compare the results in term of a patient population calculated from the permanent identification system of the hospital with the results obtained by the PMSI chaining system. No important differences have been detected and the results are very close. Besides, in order to track down possible doubles in the C.H.U patient identification system, chaining differences have been analysed. For the 22 detected cases, no identification management anomalies can be found; the doubles are the result of the calculating system of the chaining key used by the PMSI. A single interesting case can be mentioned: it concerns an anonymous registration stay; despite a previous stay in the hospital, the patient was given another identification number to ensure her complete confidentiality.

France↗

Identification in the theory and technique of psychoanalysis. Some thoughts on its farther reaches and functions.

A re-valuation of the concept of identification appears pertinent as it has come to embrace a multitude of psychoanalytic notions. Historically Freud has already differentiated meaning and function of identification. The original choice of object in the infant is coupled with his narcissism; identification is unconscious, partial and ambivalent; both determine the outcome if object loss occurs. Through identification the individual is being constituted. This notion culminated in Klein's concept of projective identification, among her other metapsychological extensions. By projecting not only phantasies and impulses but also part of its self the infant becomes capable of understanding and using symbols. Klein's notions on thought processes are commented upon here, with the inclusion of some language research, and it is argued that they are in line with Freud's paper 'Negation'. The traditional notion of identification is then extended to an 'autoplastic' one which discusses autochthonic intrapersonal processes and hereditary factors. Autoplastic identification is primary, it becomes modified by the traditionally accepted mechanism of (alloplastic) identification. A vignette from an analysis is given for illustration. The paper concludes with a discussion of the death-instinct in so far as it touches upon identification.

Cognition↗

Identification and posttraumatic stress disorder symptoms in rescue workers in the Marmara, Turkey, earthquake.

After the 1999 Marmara Turkey earthquake, 434 volunteer rescue workers and 154 soldiers who were control subjects completed the Questionnaire for Identification with Deceased Victims and the Impact of Event Scale-Revised (IES-R). Identification with the Deceased as Oneself, Identification with the Deceased as a Friend, Identification with the Deceased as a Family Member, and IES-R Intrusion, Avoidance, and Hyperarousal subscale scores were significantly higher in rescue workers. Identification-Self, Identification-Friend, and Identification-Family correlated with the IES-R subscale and total scores. The set of identification variables was significantly associated with group membership before, but not after, IES-R scores were controlled. Findings suggest that identification with deceased victims, not rescue work per se, is the risk factor of concern for posttraumatic stress disorder in rescue workers.

Adult↗

Olfactory identification deficiency and WCST performance in men with schizophrenia.

Several studies using the University of Pennsylvania Smell Identification Test (UPSIT), which requires forced choice olfactory identifications, have reported olfactory identification deficits in patients with schizophrenia. This report examines the possible links between olfactory identification (usually attributed to the orbitofrontal cortex) and executive functions (usually attributed to dorsolateral prefrontal cortex) in 24 male patients with schizophrenia and 21 male comparison subjects. Olfactory performance was investigated under two conditions: spontaneous identification and forced choice identification. Executive function was assessed with the Wisconsin Card Sorting Test (WCST). Compared with controls, patients with schizophrenia exhibited a higher average number of cigarettes smoked per day, lower spontaneous identification scores on olfactory performance, and a higher percentage of perseverative errors on the WCST; there was a significant relationship between the two performance measures. Simpson-Angus scores, neuroleptic drug treatment levels, and scores on the Positive and Negative Syndrome Scale were not correlated with either olfactory measure. The lack of correlation between the forced choice olfactory identification score and the WCST score is consistent with findings in previous studies that used the UPSIT. By contrast, use of a spontaneous identification condition to assess olfactory performance did produce a significant association with WCST performance.

Adult↗

SPECT imaging of odor identification in schizophrenia.

Deficits in olfactory identification, despite normal odor perception, are found in some neuropsychiatric disorders, including schizophrenia. We examined if regional cerebral blood flow (rCBF) differed between schizophrenia patients and controls during odor identification, hypothesizing that these brain regions could be relevant to odor identification impairments. Eight schizophrenia and eight comparison subjects provided a baseline (picture identity matching) and activation (odor identification) SPECT scan, obtained using 99mTc-HMPAO in a low dose/high dose design. Six patients and seven controls had analyzable data. MEDX data saved in ANALYZE format for SPM 95 generated paired t-test statistical data for display in Talairach space, with rCBF changes given as Z-scores. There was no schizophrenia vs. control group difference in rCBF for the baseline picture-matching test. For odor identification, schizophrenia patients had a hypometabolic right-sided cortical region that included the frontal lobe Broca's area, superior temporal lobe, and supramarginal and angular gyri. Post hoc within-group contrasts of picture-matching vs. odor identification showed that the controls significantly increased rCBF in the right-sided inferior temporal fusiform gyrus, and bilateral hippocampi and visual association areas for the odor test. The schizophrenia group showed no rCBF differences for picture-matching compared to odor identification. Patients showed significant hypometabolism in right-sided cortical areas for odor identification. They also failed to show increased rCBF in the hippocampus and visual association area, as seen in controls for odor identification compared to picture-matching. These regions may be unique to schizophrenia or have broader implications for olfactory memory retrieval.

Adult↗

Identification of trichloroethanol visualized proteins from two-dimensional polyacrylamide gels by mass spectrometry.

Proteins visualized by 2,2,2-trichloroethanol (TCE) on two-dimensional electrophoresis gels are efficiently identified by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) and MS/MS. In a previous study, a method was developed that placed TCE in the polyacrylamide gel so that protein bands can be visualized without staining in less than 5 min. A visible fluorophore is generated by reaction of TCE with tryptophan that allows for protein visualization. In this study, MALDI-TOF MS and LC-MS/MS are used to identify randomly selected Escherichia coli proteins. The identification of TCE visualized proteins is compared to the identification of Coomassie brilliant blue (CBB) stained proteins from two-dimensional gel electrophoresis of E. coli proteins. This study demonstrated that TCE visualized proteins are compatible with protein identification by MALDI-TOF peptide mass fingerprinting. For 10 randomly selected spots, TCE visualization lead to statistically significant identification of 5 proteins and CBB visualization lead to identification of 6 proteins. TCE visualized proteins are also shown to be well suited for protein identification using LC-MS/MS. In 16 spots selected for MS/MS analysis, TCE samples lead to the identification of 79 peptides; while CBB samples lead to the identification of 65 peptides. TCE samples also supported the identification of more proteins. The low stoichiometry of labeling of tryptophan residues does not require inclusion of this modification for database searches. In addition to being a rapid visualization technique compatible with MS, TCE visualization utilizes rapid washing conditions for sample preparation of proteins spots excised from polyacrylamide gels.

Amino Acid Sequence↗

Exploring the identification-production hypothesis of repetition priming in young and older adults.

The identification-production hypothesis of age-related differences in repetition priming predicts reduced priming in older adults on indirect memory measures that require stimulus production (production priming) together with age constancy on indirect measures that require stimulus identification, verification, or classification processes (identification priming). Although some evidence is consistent with this hypothesis, comparisons of identification and production priming in young and older adults often confound stimulus, subject, and dependent measure factors across tasks. Consequently, repetition priming has yet to be assessed in healthy young and healthy older adults using identification and production tasks that control for these factors. The identification-production hypothesis was tested using verb generation as a measure of production priming (Experiments 1-3) and semantic classification (Experiment 1) or semantic verification (Experiments 2 and 3) as the measure of identification priming. Contrary to the identification-production hypothesis, no age-related diminutions in identification or production forms of priming were found.

Adolescent↗

The importance of sound identification training in phonological intervention.

Little is known about the relevance of sound identification training in phonological intervention. Some treatment approaches incorporate sound identification training; others do not. The purpose of the present study was to compare articulatory improvement following treatment with and without sound identification training. Nine preschool children with severe phonological disorders were randomly assigned to 2 groups for the treatment of stimulable sound errors: (a) mixed training with concurrent production and sound identification training and (b) production-only training. Articulatory improvement was evaluated as a function of treatment type and pretraining sound identification scores. No overall difference was found between the 2 treatment types except for sounds that had been poorly identified. Articulatory errors with low identification scores made greater progress after receiving mixed training with both production and sound identification training. For error sounds receiving production training, significant relationships were found between both pre- and posttraining identification scores and articulatory improvement, suggesting (a) that perception of error sounds prior to treatment may affect degree of improvement and (b) that production training may improve perception of error sounds. Different views exist with regard to the targeting of stimulable error sounds for treatment. Results of the present study suggest that sound identification in addition to stimulability may be an important consideration in target selection as well as treatment mode.

Articulation Disorders↗

Orthonasal and retronasal odorant identification based upon vapor phase input from common substances.

Subjects were trained to identify by assigned number common substances presented as vapor phase stimuli via an orthonasal or a retronasal route. Following training, odorant identification learning was evaluated by measuring ability to correctly identify to a criterion. Those who met the criterion were then tested first with the stimuli presented to the nares that differed in location from the nares used in training, and second to the nares that corresponded in location to the nares used in training. It was found that, under conditions of natural retronasal breathing, orthonasally trained subjects made correct identifications on approximately 80% of the trials upon retronasal testing, but for the following orthonasal testing identifications were significantly more frequent, approaching 100% correct. After subsequent retronasal training, the same subjects' orthonasal identifications remained significantly higher, although identifications improved to approximately 92% correct on retronasal trials. Other subjects were instructed in a breathing technique designed to enhance retronasal stimulation. After orthonasal training, retronasal testing of these subjects still gave significantly fewer correct identifications than orthonasal testing, notwithstanding the modified retronasal breathing, but after subsequent retronasal training correct identifications by these subjects no longer differed significantly between orthonasal and retronasal testing. Efficacy of modified retronasal breathing was confirmed in two subsequent experiments. The observed substantial positive transfers between retronasal and orthonasal odorant identification training and testing loci demonstrate that these odorant pathways do not subserve completely independent olfactory systems, while the less accurate identifications via the retronasal route, unless instruction in retronasal breathing was given, suggest a difference in the efficiency with which odorants are normally delivered to the olfactory mucosa.

Adolescent↗

Evaluation of the ATB 32 C system for identification of clinical yeast isolates.

OBJECTIVE: To compare the ATB 32 C system for routine identification of clinical yeast isolates in a clinical microbiology laboratory with identification carried out by conventional methods in a mycology reference laboratory. METHODS: A total of 113 strains initially isolated at our hospital and identified in the reference laboratory were returned in duplicate, under separate code numbers, to the microbiology laboratory where the ATB 32 C system was used for identification by: 1) visual assessment of turbidity at 72 h with use of identification table; 2) visual assessment at 72 h with use of ATB 32 C analytical profile index; and 3) automatic readings with the ATB reader at 48 h and 72 h with results of growth assessments transmitted to a computer and interpreted by the ATB 32 C software. RESULTS: Visual assessment plus identification table and visual assessment plus profile index provided correct identification in 98% and 91% of strains, respectively. Visual assessment was, however, sometimes difficult and required more experience than is usually available in a routine clinical microbiology laboratory. Automatic readings with computer identification plus supplementary tests correctly identified 87% and 86% after 48 h and 72 h, respectively. CONCLUSIONS: The ATB 32 C system with automatic readings and computer identification is a satisfactory system for identification of clinical yeast isolates in a routine clinical microbiology laboratory.

Journal Article↗

Identification through X-ray fluorescence analysis of dental restorative resin materials: a comprehensive study of noncremated, cremated, and processed-cremated individuals.

Tooth-colored restorative materials are increasingly being placed in the practice of modern dentistry, replacing traditional materials such as amalgam. Many restorative resins have distinct elemental compositions that allow identification of brand. Not only are resins classifiable by elemental content, but they also survive extreme conditions such as cremation. This is of significance to the forensic odontologist because resin uniqueness adds another level of certainty in victim identification, especially when traditional means are exhausted. In this three-part study, unique combinations of resins were placed in six human cadavers (total 70 restorations). Simulated ante-mortem dental records were created. In a blind experiment, a portable X-ray fluorescence (XRF) unit was used to locate and identify the resin brands placed in the dentition. The technique was successful in location and brand identification of 53 of the restorations, which was sufficient to enable positive victim identification among the study group. This part of the experiment demonstrated the utility of portable XRF in detection and analysis of restorative materials for victim identification in field or morgue settings. Identification of individuals after cremation is a more difficult task, as the dentition is altered by shrinkage and fragmentation, and may not be comparable with a dental chart. Identification of processed cremains is a much greater challenge, as comminution obliterates all structural relationships. Under both circumstances, it is the nonbiological artifacts that aid in identification. Restorative resin fillings can survive these conditions, and can still be named by brand utilizing elemental analysis. In a continuation of the study, the cadavers were cremated in a cremation retort under standard mortuary conditions. XRF was again used to analyze retrieved resins and to identify the individuals based on restorative materials known to exist from dental records. The cremains were then processed and the analysis was repeated to determine whether restorative resins could be found under this extreme condition. Under both circumstances, sufficient surviving resin material was found to distinguish positively each individual in the study group. This study showed the utility of XRF as an analytical tool for forensic odontology and also the significance of the role of restorative resins in victim identification, even after cremation.

Bone and Bones↗

Superiority of molecular techniques for identification of gram-negative, oxidase-positive rods, including morphologically nontypical Pseudomonas aeruginosa, from patients with cystic fibrosis.

Phenotypic identification of gram-negative bacteria from Cystic Fibrosis (CF) patients carries a high risk of misidentification. Therefore, we compared the results of biochemical identification by API 20NE with 16S rRNA gene sequencing in 88 gram-negative, oxidase-positive rods, other than morphologically and biochemically typical P. aeruginosa, from respiratory secretions of CF patients. The API 20NE allowed correct identification of the bacterial species in 15 out of 88 (17%) isolates investigated. Agreement between the API and the 16S rRNA gene sequencing results was high only in isolates with an API result classified as "excellent identification". Even API results classified as "very good identification" or "good identification" showed a high rate of misidentification (67% and 84%). Fifty-two isolates of morphological and biochemical nontypical Pseudomonas aeruginosa, representing 59% of all isolates investigated, were not identifiable or misidentified in the API 20NE. Therefore, rapid molecular diagnostic techniques like real-time PCR and fluorescence in situ hybridization (FISH) were evaluated in this particular group of bacteria for identification of the clinically most relevant pathogen, P. aeruginosa. The LightCycler PCR assay with a P. aeruginosa-specific probe showed a sensitivity and specificity of 98.1% and 100%, respectively. For FISH analysis, a newly designed P. aeruginosa-specific probe had a sensitivity and specificity of 100%. In conclusion, molecular methods are superior over biochemical tests for identification of gram-negative, oxidase-positive rods in CF patients. In addition, real-time PCR and FISH allowed identification of morphologically nontypical isolates of P. aeruginosa within a few hours.

Cystic Fibrosis↗

Identification, evaluation, and management of obesity in an academic primary care center.

BACKGROUND: The rapidly increasing prevalence of obesity among children is one of the most challenging dilemmas facing pediatricians today. While the medical community struggles to develop effective strategies for the treatment of this epidemic, timely identification of obesity by pediatric health care providers remains the crucial initial step in the management of obesity. OBJECTIVE: Direct assessment of pediatric clinicians' performance in identifying and managing obesity in clinical practice has not been conducted to date. The objective of this study was to determine rates of identification of obesity by pediatric residents, nurse practitioners, and faculty members in an academic primary care setting and to describe the actions taken by these providers in their evaluation and management of obesity. DESIGN: A retrospective medical record review of all health supervision visits for children 3 months to 16 years of age, examined between December 1, 2001, and February 28, 2002, was performed. For children <5 years of age, a weight >120% of the 50th percentile of weight-for-height was defined as obese. For children > or =5 years of age, a body mass index of >95th percentile for age and gender was defined as obese. SETTING: A large, primary care practice located in a tertiary-care, academic, pediatric hospital, which serves a predominantly urban, minority (70% African American), Medical Assistance-insured (90%) population. PARTICIPANTS: Primary care providers, including pediatric residents, nurse practitioners, and faculty physicians. RESULTS: Of the 2515 visits reviewed, a total of 244 patients met the study definition of obesity, yielding an estimated prevalence of obesity visits of 9.7% among health supervision visits for children 3 months to 16 years of age. This prevalence of obesity visits cannot be used to estimate the population prevalence of obesity, given the skewed distribution of visits toward very young children. For all children who met the study definition of obesity, providers documented obesity in their assessments in only 53% of the reviewed visits (129 visits). Although the majority of charts (69%) contained an adequate dietary history, only 15% included a description of the child's activity level or television viewing. Obesity was noted in the physical examination in 39% of cases. For children for whom obesity was identified as a problem by their clinicians (129 patients), 81% of charts contained an adequate dietary history, whereas 27% contained a description of the child's activity level or television viewing. Obesity was noted in the physical examination in 64% of cases. Most children identified as obese by their providers received some management specific to their obesity, including education, screening, and specialist referral. Dietary changes were recommended for 71%, increased activity for 33%, and limitation of television viewing for only 5%. Eighty-three percent of providers recommended close follow-up monitoring. Other recommendations included referral to a dietitian (22%), screening laboratory studies (13%), a food diary (9%), endocrine referral (5%), or preventive cardiology referral (3%). Provider identification of obesity was affected by the age of the patient and by the degree of obesity. Obesity identification was lowest among preschool children (31%) and highest among adolescent patients (76%). Providers evaluating older and heavier children were also more likely to obtain activity histories, note obesity in the physical examination, recommend changes in activity, refer the patient to a nutritionist, obtain screening laboratory studies, and recommend close follow-up monitoring. Identification of obesity and other outcome variables were not significantly influenced by the level of provider training or patient gender. CONCLUSIONS: Although the prevalence of childhood obesity has now reached epidemic proportions, it was under-recognized and under-treated by pediatric primary care providers in our study. Providers identified obesity as a problem for only one-half of the obese children examined for health supervision. The lowest rates of obesity identification occurred among children <5 years of age and those with milder degrees of obesity. Identification did not improve with additional years of pediatric training. Even for the subset of children identified as obese by their providers, evaluation and treatment often were not consistent with current recommendations. For example, more attention was given to the role of diet, compared with activity, in the evaluation of obesity. In particular, only a small number of providers (5%) recommended a decrease in television viewing to their obese patients, despite evidence linking television viewing and pediatric obesity. This finding is of concern, because obesity is known to be a multifactorial disease that responds only to significant changes in both dietary and activity behaviors. Only 13% of providers requested laboratory studies as part of their recommendations. The American Academy of Pediatrics currently recommends obtaining a lipid profile, total cholesterol level, and screening test for type 2 diabetes mellitus as part of the evaluation of obesity. The majority of clinicians who requested laboratory studies included thyroid function tests, which are not recommended by the American Academy of Pediatrics because of the very low likelihood of hypothyroidism as a cause of obesity. Although the extent of evaluation and management for children who were recognized as obese did not meet current guidelines, it was far better than that for patients who were not identified as obese by their providers. This demonstrates the importance of timely identification as the crucial initial step in the management of obesity. The results of this study are disheartening, especially as evidence mounts regarding the importance of early intervention in preventing the medical and psychosocial sequelae of obesity, as well as the persistence of obesity into adulthood. This study highlights the need for increased awareness and identification of obesity in the primary care setting, especially among younger children and those with mild obesity.

Academic Medical Centers↗

Reaction time and critical duration for lines' detection and identification of their orientation.

Reaction time (RT) and duration of complete temporal summation (tc) were studied for both detection of lines and identification of their orientation. The RT was studied as a function of stimulus intensity, number of orientations and angular difference between them. The temporal summation was studied for detection and identification at several angular differences. It was found that the intensity-dependent part of RT for identification at angular difference of 22 degrees equaled that for detection but considerably lengthened at smaller angular differences (10 degrees). The intensity-independent part of RT was minimal for detection and maximal for identification with eight alternatives. The tc increased from 30 msec for detection and identification with the accuracy of 22 degrees to 100 msec for identification with the accuracy of 5 degrees. The results support the conclusion that detection and coarse identification differ only in their later stages and that accurate identification involves additional processes with longer time-constants compared to coarse identification.

Humans↗

[Forensic odontological identification of disaster victims. Experience with the disaster of the Martinair DC-10 in Faro, Portugal].

Forensic odontology plays an important, often decisive role in the identification of disaster victims. In the Dutch situation, the forensic odontologist is part of the Disaster Victim Identification (DVI)-team of the Dutch National Police Force. The extensive experience with forensic odontological identification of disaster victims, gained within the Dutch DVI-team is described and illustrated with reference to the identification procedure following the Martinair DC-10 aircraft crash at Faro, Portugal, december 21st, 1992. In the identification of the total of 56 victims, forensic odontology contributed in 55 cases (98.2%) to the identification; in 40 cases there were such clear agreements that identification based on forensic odontology alone would have been sufficient. The success of the forensic odontological identification method appeared to be greatly dependent of the adequate cooperation of dentists that made their treatment data available for comparison.

Aircraft↗

A computational method for assessing peptide- identification reliability in tandem mass spectrometry analysis with SEQUEST.

High-throughput protein identification in mass spectrometry is predominantly achieved by first identifying tryptic peptides by a database search and then by combining the peptide hits for protein identification. One of the popular tools used for the database search is SEQUEST. Peptide identification is carried out by selecting SEQUEST hits above a specified threshold, the value of which is typically chosen empirically in an attempt to separate true identifications from false ones. These SEQUEST scores are not normalized with respect to the composition, length and other parameters of the peptides. Furthermore, there is no rigorous reliability estimate assigned to the protein identifications derived from these scores. Hence, the interpretation of SEQUEST hits generally requires human involvement, making it difficult to scale up the identification process for genome-scale applications. To overcome these limitations, we have developed a method, which combines a neural network and a statistical model, for normalizing SEQUEST scores, and also for providing a reliability estimate for each SEQUEST hit. This method improves the sensitivity and specificity of peptide identification compared to the standard filtering procedure used in the SEQUEST package, and provides a basis for estimating the reliability of protein identifications.

Algorithms↗