Mutagenic effects of combinations of chemical carcinogens and environmental pollutants in mice as shown by the micronucleus test.
The effects of combinations of the chemical carcinogens, dimethylnitrosoamine (DMN) or N-methyl-N'-nitro-N'-nitrosoguanidine (MNNG), with the environmental pollutants, cadmium (Cd) or polychlorinated biphenyls (PCB) were examined by Schmidt's micronucleus method with ddY strain mice. DMN had a positive mutagenic effect by oral administration, with a dose response between 25 and 100 mg/kg body weight. MNNG gave a slight positive, though not consistent result, and seemed to be more sensitive at an earlier stage after administration of the drug. Cd was given for up to 7 days in drinking water as a solution of CdCl2 at concentrations of 3, 30 or 300 ppm as Cd. PCB was administered orally at 100 mg/kg daily for 6 days as a solution in corn oil, or subcutaneously injected in ethanol. Neither Cd nor PCB gave positive results in the micronucleus test. However, a synergistic positive effect of Cd with DMN was seen, but PCB with either of these nitrosamines was not positive.