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At least 307 records · Page 17Linked to original sources
PubMed1991

Isolation and ultrastructural analysis of microfibrillar structures from foetal bovine elastic tissues. Relative abundance and supramolecular architecture of type VI collagen assemblies and fibrillin.

Extensive intact assemblies of matrix macromolecules have been solubilized from foetal calf skin, nuchal ligament and aorta by a new procedure that includes bacterial collagenase digestion under non-reducing, non-denaturing conditions and gel filtration chromatography. Type VI collagen was identified as the major microfibrillar element of these tissues by SDS-PAGE analysis and Western blotting. Rotary shadowing electron microscopy of these preparations revealed by far the most abundant and extensive arrays of intact collagen VI microfibrils isolated to date. The distinct microfibrillar species, fibrillin, which was identified on the basis of its periodicity and morphology, was also solubilized in abundance by this protocol. Analysis of these complex polymers has generated new information on their supramolecular architecture and relative abundance in these tissues. The protocol also demonstrates that the release of intact collagen VI microfibrils from these tissues is largely dependent on the removal of the major collagen fibrils.

Animals↗
PubMed1977

Preparation of a specific antiserum towards the microfibrillar protein of elastic tissues.

The microfibrillar protein (MFP) of bovine foetal ligamentum nuchae was prepared and used to induce an antiserum (I) in rabbit. Antibody (I), reacted with a dispersion of MFP in Ouchterlony plates, produced one major precipitin band (A) and two minor bands (B and C). The major band (A) was used to prepare antiserum (II) in a second rabbit. Antibody (II) was monospecific for precipitin band (A) and was shown to be selectively directed against MFP of bovine and chick elastic fibres by immunofluorescent and immunoperoxidase techniques. Immunoperoxidase-labelling techniques demonstrated the regular organization of microfibrillar protein on the surface of elastic fibres and suggested the ability of MFP to assemble into a framework defining the three dimensional polymerization of elastin monomers during the formation of elastin fibres. It was observed that collagen fibrils, which were in close association with the microfibrillar protein on the surface of elastic fibres, also possessed a protein which was immunologically related to MFP.

Animals↗
PubMed1982

Isolation and partial characterization of an elastase-type protease in human vulva fibroblasts: its possible involvement in vulvar elastic tissue destruction of patients with lichen sclerosus et atrophicus.

A complete disappearance of orcein positive material was observed in the superficial dermis of patients suffering from Lichen sclerosus et atrophicus. An elastase-type protease was isolated and partially purified from Triton X-100 extracts of human vulvar fibroblasts by gel permeation chromatography. It presents the characteristics of a metalloenzyme hydrolyzing Succinoyl-tri-alanine paranitroanilide maximally at pH 8.0 and is also active towards insoluble elastin. When partially purified enzyme is directly applied on to rabbit skin sections or when injected intradermally to young rabbits, it produces appreciable degradation of elastic fibers. The involvement of this protease in the disappearance of elastic fibers in Lichen sclerosus et atrophicus is postulated.

Adult↗
PubMedDate not supplied

Localisation of endothelin-1 in rat aortae, the relationship to flow and elastic tissue tears.

The aortic expression of endothelin-1 (ET-1) was examined in three species of rat using a novel en face immunohistochemical technique. A genetically hypertensive strain was compared to two normotensive strains, one of which is known to develop spontaneous lesions within the abdominal aorta. ET-1-positive staining was increased about the major aortic branch ostia and over the dorsal abdominal aortic wall in all three species indicating a flow-related expression pattern. Mitotic and hyperchromatic endothelial cells stained strongly for ET-1 as did occasional multi-nucleated endothelial cells. The aortic-lesion-prone normotensive strain developed transverse tears of the internal elastic lamina with a corresponding endothelial cell response. Endothelium at the edge of these lesions was strongly stained for ET-1 and appeared to be associated with increased leucocyte adhesion as did other strongly ET-1-stained areas in all three species. This study indicates that increased ET-1 expression is anatomically localised within the rat aorta, possibly by haemodynamic stress. This may have implications for maintaining endothelial cell confluence, aortic smooth muscle cell reparative processes and possibly eventual pathophysiological conditions such as atherosclerosis.

Animals↗
PubMed1998

Force heterogeneity in a two-dimensional network model of lung tissue elasticity.

We have developed a model of forces developed in lung tissue in which the stress-bearing units are heterogeneous. Each element of the fiber network is composed of an idealized elastin and collagen element in parallel. Elastin is represented by linear springs and collagen by stiff strings that extend without resistance until taut. The model can quantitatively account for the nonlinear shape of the length-tension curve of lung tissue strips when the knee lengths of the collagen fibers are distributed according to an inverse power law. The novel feature of this model is that as macroscopic strain increases the load is carried by progressively fewer elements with progressively higher forces, and preferential pathways of force transmission emerge within the matrix. The topology of these self-organizing pathways of force transmission takes the rough appearance of cracks, but, unlike real cracks, they represent the locus of force concentration rather than force release.

Collagen↗