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PubMed1996

Differential expression of tenascin by astrocytes associated with the supraoptic nucleus (SON) of hydrated and dehydrated adult rats.

The present study evaluated the expression of tenascin by astrocytes in the supraoptic nucleus and associated ventral glial limitans (SON-VGL) under conditions that induce reversible changes in neuronal organization (dehydration and rehydration). Immunostaining of astroglia cultured from rat neonatal SON-VGL confirmed that these cells are capable of both expressing and secreting tenascin. Observations of immunostained tissue sections from adult rats revealed tenascin immunoreactivity primarily in the VGL and dendritic zone, subjacent to SON neuronal somata. Comparison of immunostained tissues from hydrated and dehydrated animals showed an apparent decrease in the intensity of immunostaining with dehydration. Subsequent Western blots of similar tissues confirmed the presence of the 210-220-kDa tenascin protein in the SON-VGL. SON-VGL tissues from control, dehydrated, and rehydrated rats were then studied by using SDS-PAGE and quantitative gel densitometry. A consistent decrease in tenascin concentration was observed by 6 days of dehydration that, with rehydration, reversed back toward or beyond control levels. Together, these observations indicate that SON-VGL astrocytes variably express tenascin and that this protein may play a role in adult SON plasticity.

Animals↗
PubMed2001

Plasticity of neurohypophysial terminals with increased hormonal release during dehydration: ultrastructural and biochemical analyses.

Arginine vasopressin- (AVP) and oxytocin- (OXT) secreting magnocellular neurons undergo gross structural changes with chronic physiological stimulation. Here, we investigated subcellular aspects of plasticity in rat neurohypophysial terminals during dehydration. Ultrastructural analyses demonstrated that chronic dehydration by 2% NaCl drinking for 7 days significantly decreased the numbers of neurosecretory granules and microvesicles but not the numbers of mitochondria. Moreover, in dehydrated rats, terminals making neurovascular contacts enlarged, whereas terminals in apposition to astrocytes, i.e., neuroglial contacts, became smaller. Western blot analyses demonstrated significant decreases in the levels of F3 and Thy-1 together with those of AVP- and OXT-neurophysin, but the levels of synaptophysin, SNAP-25, and GAP-43 were unchanged. Both F3 and Thy-1 were recovered in the buffer-insoluble pellet, and phosphatidyl inositol-specific phospholipase C treatment released both molecules from the crude membrane fraction, indicating that they are attached to terminal membranes by glycosylphosphatidyl inositol anchors. Confocal microscopic observations demonstrated that F3 colocalized with Thy-1 in the same terminals of magnocellular neurons. In contrast, the level of calretinin, a Ca(2+) binding protein was significantly increased with chronic dehydration. Thus, the present results suggest that enhancement of neurovascular contacts results from rearrangement of terminal-astrocyte and terminal-vessel contacts rather than enlargement or sprouting of magnocellular terminals themselves. The down-regulation of F3 and Thy-1 may contribute to enhancement of neurovascular contacts that accompany increased peptide release during dehydration.

Animals↗
PubMedDate not supplied

Therapeutic strategies for prevention of sickle cell dehydration.

The intracellular concentration of Hb S is an important determinant of the kinetic of polymer formation and cell sickling. A variable fraction of dense, dehydrated erythrocytes with high Hb S concentration is seen in the blood of patients with sickle cell disease; these dense cells play an important role in the pathophysiology of the vasoocclusive events of sickle cell disease, due to their higher tendency to polymerize and sickle. Sickle cell dehydration is due to loss of K+, Cl-, and water: the two major determinant pathways of dehydration of sickle erythrocytes are the Ca2+-activated K+ channel (IK1 or Gardos channel) and the K-Cl cotransport (KCC). Specific inhibitors of these pathways being tested in patients with sickle cell disease are Mg2+ pidolate, which inhibits KCC by increasing the sickle cell content of Mg2+, and clotrimazole and derivatives of clotrimazole metabolites, which specifically block the Gardos channel. An inhibitor of Cl- conductance has been shown to reduce dehydration in a transgenic mouse model of sickle cell disease but has not been tested in humans. If clinical efficacy and benefit are demonstrated, an inhibitor of cell dehydration could be used in patients as a single agent or in combination with existing therapies, such as hydroxyurea.

Anemia, Sickle Cell↗
PubMedDate not supplied

Green tea extract and aged garlic extract inhibit anion transport and sickle cell dehydration in vitro.

Both green tea extract (GTE or tea polyphenols) and aged garlic extract (AGE) effectively inhibited in vitro dehydration of sickle red blood cells induced by K-Cl cotransport or red cell storage. For K-Cl cotransport induced by 500 mM urea, 0.3 mg/ml EGCg (epigallocatechin gallate; a major component in GTE) almost completely inhibited dehydration, and 6 mg/ml AGE inhibited dehydration to 30% of the control level. Both vitamins E and C had no effect at the level of 2 mM. Different tea extracts had different degrees of inhibition, but the inhibitory activity increased when the number of hydroxyl groups in the compounds increased. With storage of sickle cells at 4 degrees C for 6 days, the cells started to undergo spontaneous dehydration when incubated at 37 degrees C. Neither inhibitors for Ca-induced K efflux nor K-Cl cotransport could inhibit cell dehydration of stored sickle cells, but both GTE and AGE effectively inhibited it. Chloride efflux measurements using a chloride electrode demonstrated that both GTE and AGE inhibited anion transport in red blood cells. The inhibitory mechanism of these compounds may be related to anion transport inhibition, although involvement of their antioxidant activities can not yet be ruled out.

Anemia, Sickle Cell↗
PubMed1997

De novo protein biosynthesis responses to water stresses in wood frogs: freeze-thaw and dehydration-rehydration.

Protein biosynthesis responses occurring during the postthaw (after 12 h freezing at -1.4 degrees C), dehydration (to 27 or 40% of total body water lost), or rehydration (after the loss of 40% of body water) were monitored in tissues of spring-collected wood frogs (Rana sylvatica) after intraperitoneal injection of 35S-labeled methionine + cysteine. All six organs tested accumulated radiolabeled amino acids and organs of both thawing and rehydrating frogs held at 3-5 degrees C showed a linear increase in amino acid incorporation into the acid-precipitable protein fraction over time. By contrast, dehydrating animals showed little or no increment in protein bound radioactivity over the course of the stress, a result that may be indicative of metabolic suppression in organs when dehydration became severe. Isoelectrofocusing (IEF) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) were used to characterize the proteins synthesized by liver under each experimental state. IEF revealed both new peaks of 35S-labeled proteins and enhanced labeling of others in extracts from experimental animals, compared with controls. In particular, new synthesis of proteins with isoelectric points of about 6.0 was prominent and labeled proteins in this IEF peak persisted at 5, 10, or 24 h postinjection, becoming proportionally more important over time. SDS-PAGE analysis of the p(I) 6.0 peaks from thawed, dehydrated, and rehydrated frogs revealed the presence of one major low molecular weight protein in each case with molecular masses of 15, 13, and 21 kDa, respectively. These data indicate that the biochemical adaptations supporting freeze tolerance and dehydration tolerance in anurans include the stress-induced biosynthesis of a suite of proteins including the novel synthesis of selected specific proteins. These proteins may represent stress-related (or shock) proteins or may have specific roles in metabolic adaptation in each state such as in water and ionic balance or cell volume regulation.

Animals↗
PubMed1996

Renal function and organic anion and cation transport during dehydration and/or food restriction in chickens.

The effect of dehydration in the presence or absence of continued food intake on renal function was evaluated in chickens. In addition, renal transport of organic anions and cations under these conditions was assessed in vitro by uptake of 14C-para-aminohippuric acid and 14C-tetraethylammonium bromide by renal slices. Water restriction with continued food intake resulted in increases in serum osmolality and serum concentrations of sodium, uric acid, calcium and total protein. If food was restricted in addition to water, only serum osmolality and sodium concentration were significantly increased after 48 hours. Dehydration with continued access to food resulted in marked decreases in extracellular fluid volume, glomerular filtration rate and effective renal plasma flow. If food was restricted during dehydration, the decrease in effective renal plasma flow was attenuated despite reductions in glomerular filtration rate and extracellular fluid comparable to that seen in dehydrated birds allowed free access to food. Transport of organic anions was significantly increased after 24 and 48 hours of water restriction, regardless of whether food was withheld. Enhanced transport of organic anions in the presence of decreased glomerular filtration rate and effective renal plasma flow during dehydration may promote precipitation of urates and nephrosis in chickens.

Animals↗
PubMed1988

Precedence of head homoeothermia over trunk homoeothermia in dehydrated men.

Three male humans were subjected repeatedly to 20 min exercise on a bicycle ergometer: twice when hydrated normally and twice when dehydrated. Tympanic (Tty) and oesophageal (Tes) temperatures were recorded and sweat rates on forehead and back were measured. Dehydration did not change the forehead sweat rate, but on the back it reduced significantly, resulting in an increase of Tes. However, Tty was decreased by dehydration. 20 min after the end of exercise subjects were allowed to drink water in order to trigger the potohidrotic response. A potohidrotic response was noted on the back of dehydrated subjects only. It is concluded that dehydration results in active inhibition of sweating on the body but not on the forehead, where evaporation is needed for selective cooling of the brain.

Body Temperature↗
PubMed1986

Effect of dehydration and rapid rehydration on renal function and on plasma renin and aldosterone levels in the black Bedouin goat.

Bedouin goats in the extreme deserts of the Middle East are regularly subjected to severe dehydration and possess a capacity to rapidly rehydrate by drinking large volumes of water. Urine flow, glomerular filtration rate (GFR) and effective renal plasma flow (ERPF) in the fully hydrated animals amounted to 0.74 +/- 0.4 ml X min-1, 76 +/- 29 ml X min-1 and 344 +/- 146 ml X min-1 respectively. In goats that were dehydrated to a loss of about 30% of their initial body weight, urine flow dropped to 24% of the value recorded in the hydrated animals and GFR and ERPF dropped to half their level recorded in the hydrated phase. Na and K+ excretion decreased in the water depleted goats and further decrease was recorded following drinking. Following drinking the urine flow, GFR and ERPF of the recently rehydrated goats dropped to below the rates recorded in the dehydrated animals. During the 3 h of the continuous recording that followed the drinking, all three rates did not exceed the predrinking level. Plasma renin activity amounted to 0.37 +/- 0.32 ng AI X ml-1 X h-1 in the hydrated animals. In dehydrated ones it amounted to 4.8 +/- 2.8 ng AI X ml-1 X h-1 and a further increase was recorded following drinking. Aldosterone in the hydrated goats was 5.5 +/- 4.3 ng% and increased to 13.9 +/- 2.3 ng% in the dehydrated animal and amounted to 20.1 +/- 5.5 ng% 2 h following drinking.(ABSTRACT TRUNCATED AT 250 WORDS)

Aldosterone↗
PubMed1997

Response to dehydration in elderly patients in long-term care.

Elderly patients in long-term care have a high plasma osmolality (pOSM) and associated increased mortality. In these patients, we examined pOSM, thirst (visual analogue scale, VAS) and arginine vasopressin (AVP) response to 16-hour dehydration and 2-hour rehydration. Twelve patients were randomly divided into two groups (A & B). Group A had their fluid balance monitored for two weeks, group B did not. Single measurements of VAS, pOSM and AVP were conducted on days 0, 7 and 13. Dehydration tests were conducted in both groups at the start of the study (DAY 1), and at the end of the two-week period (DAY 14). There were no significant differences between group A and B, so the results are presented for the two groups combined. There was a significant increase in both pOSM and VAS during dehydration, and a significant fall in both variables during rehydration [F (9,99) = 18.69, p < 0.001, and F(9,99) = 11.25, p < 0.001, respectively]. Plasma AVP did not change significantly during either dehydration or rehydration [F (9,99) = 0.59, p = 0.8]. There were no significant differences in response for any of the three variables between DAY 1 and DAY 14. We conclude that elderly patients in long-term care exhibit changes in thirst and osmolality during dehydration and rehydration, and that these responses are reproducible. The lack of a significant AVP response requires further investigation.

Aged↗
PubMed2004

The novel gene CpEdi-9 from the resurrection plant C. plantagineum encodes a hydrophilic protein and is expressed in mature seeds as well as in response to dehydration in leaf phloem tissues.

The resurrection plant Craterostigma plantagineum Hochst. is used as an experimental system to investigate desiccation tolerance in higher plants. A search for genes activated during early stages of dehydration identified the gene CpEdi-9, which is expressed in mature seeds and in response to dehydration in the phloem cells of vascular tissues of leaves. Elements for the tissue-specific expression pattern reside in the isolated promoter of the CpEdi-9 gene, as shown through the analysis of transgenic plants. The CpEdi-9 promoter could be a suitable tool for expressing genes in the vascular system of dehydrated plants. CpEdi-9 encodes a small (10 kDa) hydrophilic protein, which does not have significant sequence homologies to known genes. The predicted protein CpEDI-9 shares some physicochemical features with LEA proteins from plants and a nematode. Based on the unique expression pattern and on the nucleotide sequence we propose that CpEdi-9 defines a new class of hydrophilic proteins that are supposed to contribute to cellular protection during dehydration. This group of proteins may have evolved because desiccation tolerance requires the abundant expression of protective proteins during early stages of dehydration in all tissues.

Abscisic Acid↗
PubMed1992

Ovine maternal and fetal renal vasopressin receptor response to maternal dehydration.

OBJECTIVE: Arginine vasopressin secretion increases in response to increased plasma osmolality or hypovolemia. Dehydration-induced increases in plasma arginine vasopressin levels have been shown to down-regulate arginine vasopressin V2 receptors in adult rat kidneys. Our study determined ovine maternal and fetal renal arginine vasopressin receptor characteristics and receptor response to maternal dehydration. STUDY DESIGN: Eight pregnant ewes (113 +/- 1 days) were dehydrated for 72 hours; eight animals served as controls. Renal medullary tissue was isolated from maternal and fetal kidneys, and arginine vasopressin receptor characteristics determined with saturation and competition assays using tritiated arginine vasopressin, arginine vasopressin, and arginine vasopressin analogs. RESULTS: Euhydrated maternal and fetal renal medullary arginine vasopressin receptor dissociation constant (3.0 +/- 0.3 and 1.9 +/- 0.3 nmol/L) and maximal binding capacity (149 +/- 15 and 111 +/- 33 fmol/mg protein) values were similar. Pharmacologic profiles with selective agonists indicated a predominance of V2 receptors. Dehydration significantly increased maternal and fetal plasma osmolalities (304 +/- 2 to 320 +/- 2; 296 +/- 1 to 319 +/- 3 mOsm/kg water, respectively) and arginine vasopressin levels (3.8 +/- 1.4 to 29.3 +/- 4.6; 4.4 +/- 1.0 to 16.9 +/- 5.0 pg/ml, respectively) but had no effect on arginine vasopressin receptor binding. CONCLUSION: Specific, saturable, single-site tritiated arginine vasopressin binding is present in ovine maternal and fetal renal medullary membranes. Ovine maternal and fetal renal arginine vasopressin receptors do not down-regulate in response to dehydration-induced elevations in plasma arginine vasopressin levels.

Animals↗
PubMed1985

Neuronal/glial plasticity in the supraoptic dendritic zone in response to acute and chronic dehydration.

The magnocellular neurosecretory cells of the supraoptic nucleus increase production and secretion of oxytocin and/or vasopressin in response to dehydration, gestation and lactation. Dynamic neuronal/glial interactions have also been shown to occur in response to these stimuli, resulting in a reversible increase in soma-somatic direct membrane apposition at these times. Chronic (lactation, 10 days of saline drinking) but not acute stimuli (4-24 h water deprivation) are further accompanied by the reversible formation of axo-somatic double synapses (one presynaptic terminal contacting two postsynaptic elements), which are virtually absent in control animals. The dendrites of these cells course ventrolaterally toward the ventral glial lamina, and have also been shown to be involved in this plasticity: dendro-dendritic direct membrane apposition and axo-somatic double synapses significantly vary with gestation and parturition. The present study investigated the dendritic zone response to both chronic and acute dehydration and rehydration. Increased dendro-dendritic membrane contacts resulted from both stimuli. Rehydration following acute dehydration resulted in a dose-dependent return to control levels, while rehydrated chronic dehydrates did not show such a return until 35 days of rehydration. The percentage of dendrites contacted by double synapses did not vary with treatment, and there were no sex differences. The recalcitrance on the part of the dendrites to return to normal following chronic dehydration may reflect a readiness to respond to renewed hormone demand.

Acute Disease↗
PubMed1995

Effects of dehydration and salt-loading on hypothalamic vasopressin mRNA level in male and female rats.

Experiments were carried out on 9- to 11-week-old male and female Sprague-Dawley rats. Dot-blot analysis and 3'-end digoxigenin-labeled 26mer oligonucleotide probe were used in the investigation of vasopressin (AVP) mRNA level in the hypothalamus of male and female rats. The normal hypothalamic AVP-mRNA level in males was 48% higher than that in females (P < 0.05). Plasma osmolality was also higher in males than in females (P < 0.05). In dehydrated rats, the hypothalamic AVP-mRNA level was 2.47 and 1.98 times in females (P < 0.001) and males (P < 0.01), respectively, as much as in their normal controls; the difference in hypothalamic AVP-mRNA level between dehydrated females and males was statistically insignificant. Plasma osmolality was higher in dehydrated females than in dehydrated males (P < 0.01). In salt-loaded rats, hypothalamic AVP-mRNA level was 2.47 and 2.17 times in females (P < 0.001) and males (P < 0.01), respectively, as much as in their controls. The difference in hypothalamic AVP-mRNA level between salt-loaded males and females was not statistically significant. Plasma osmolality in salt-loaded females was also higher than that in salt-loaded males (P < 0.001). These findings indicate that there is sex difference in hypothalamic AVP mRNA level and plasma osmolality under normal conditions; during dehydration and salt-loading AVP mRNA level increases and the difference in AVP mRNA level between males and females becomes insignificant.

Animals↗
PubMed1984

Plasma prolactin during the body fluid and electrolyte changes of dehydration and sodium depletion in steers.

The effect of dehydration and sodium depletion on plasma prolactin levels in steer calves is very different from the changes seen in the rat and possibly in man. Removal of drinking water was followed by progressive dehydration for 96 h during which time packed cell volume (PCV) increased from 39.9% to 44.7% and plasma osmolarity (pOsm) rose from 303.3 mOsm to 342.0 mOsm/l with hypernatraemia. At the same time plasma prolactin ( pPRL ) was rapidly reduced from a basal value of 2.3 ng/ml to barely measurable amounts and remained low during dehydration. Restoration of ad lib drinking water was followed by rapid reduction of PCV and pOsm to sub-basal levels during which time the pPRL increased significantly to persist at 15 ng/ml. Sodium depletion was produced by continuous loss of sodium-rich saliva from unilateral fistulation of a parotid duct. During sodium deficiency PCV increased from 38.6% to 45.6% but pOsm fell significantly from 299.9 mOsm/l to 286 mOsm/l with hyponatraemia. As in dehydration, during sodium depletion pPRL was suppressed, and after 7 days was reduced from a basal level of 5.4 ng/ml to 0.5 ng/ml. The sodium depleted steers when given 0.3M NaHCO3, which they consumed readily to restore sodium homeostasis, restored the deficiency gradually in 5 days when pPRL , pOsm and PCV all returned to basal levels without any 'overshoot' or hypersecretion of pPRL . Our finding indicate that extracellular fluid volume changes, not electrolyte content, affect pPRL . This is in agreement with results obtained in the rat, and possibly in man, but the fact that in the steer, the endogenous changes in prolactin level show a profound reduction provides an extreme example of species difference. The means whereby both divergent physiological processes of dehydration and sodium depletion generate stimuli which inhibit prolactin secretion and the relevance of this response in fluid balance homeostasis requires further research.

Animals↗
PubMed1996

Plasma endothelin-1 level in athletes after exercise in a hot environment: exercise-induced dehydration contributes to increases in plasma endothelin-1.

We investigated whether dehydration due to exercise contributes to the increase in plasma endothelin-1 (ET-1) concentration. We measured the plasma concentration of ET-1 before and after exercise in a hot environment (about 30 degrees C). Five male intercollegiate Kendo (Japanese fencing) players entered the present study. Each athlete participated in 15 min of Kendo fighting, followed by 5 min of rest and another 15 min of Kendo fighting (i.e., total exercise 30 min), with or without oral intake of 700 ml of water. Body weight and left atrial diameter, a parameter that reflects changes in circulating plasma volume, were significantly decreased after exercise under both conditions. However, the decreases in both values were significantly greater after exercise without water intake than after exercise with water intake, indicating that dehydration and decreased circulating plasma volume were more marked after exercise without water intake. The extent of the increase in plasma ET-1 concentration appeared to be closely related to the extent of exercise-induced dehydration; the greater the dehydration, the greater the increase in plasma ET-1 concentration. These findings suggest that exercise-induced dehydration may contribute to increases in plasma ET-1 concentrations.

Adult↗
PubMedDate not supplied

Summating-potential/action-potential ratio in normal ears: effects of dehydration.

INTRODUCTION: Studies using electrocochleography (ECoG) in patients with Meniere's disease and in patients with perilymph fistula have demonstrated abnormally large summating-potential (SP) components compared with the amplitude of the action-potential (AP). The possible influence of normal physiological variation, methodological differences, and test-retest variations are often difficult to interpret. The purpose of this study is to specify what constitutes a clinically important change in the SP/AP ratio in normal hearing subjects under the condition of dehydration induced by urea. MATERIALS AND METHODS: Baseline ECoG was performed on 20 normal hearing volunteer subjects after an 8 hour fast. Urea, 20 g, was administered orally, following which recordings were made at 60 and 90 minutes. The majority of subjects were found to have maximal SP/AP changes at 60 minutes post dehydration; therefore, only these results were included in the final analysis. Subsets of data were statistically evaluated. RESULTS: In all ears but one, the preingestion SP/AP ratio was less than or equal to 0.37. Male and female data were shown to comprise separate populations both before and following dehydration. The 95% upper limit for baseline SP/AP ratio was 0.39 for males and was 0.25 for females. The gender-related difference remained after dehydration. The mean male SP/AP ratio showed a statistically significant but slight decrease whereas the female SP/AP ratio was unaltered. CONCLUSION: These data demonstrate a statistically significant gender-related difference in SP/AP ratio in normals. The upper limit of normal is 0.39 for males and 0.25 for females. A basis for the gender difference is puzzling and requires further investigation. Dehydration produces a statistically significant reduction in SP/AP ratio in men. This change was not observed in women.

Action Potentials↗
PubMed1994

Structural dynamics of neural plasticity in the supraoptic nucleus of the rat hypothalamus during dehydration and rehydration.

It has been known that magnocellular neuroendocrine cells (MNCs) of mammalian hypothalamus show structural plasticity in response to chronic osmotic stimulation. In this study, we investigated the relationships among plasma osmolarity and several structural changes such as alterations of soma size, juxtaposition, and synapses of the supraoptic nucleus (SON) in the rat hypothalamus during dehydration and rehydration. Male rats were osmotically stimulated by supplying with 2% NaCl solution instead of tap water for 10 days, and then they were rehydrated with tap water. Plasma osmolarity was gradually elevated with progress of salt loading and returned to control level on the seventh day of rehydration. Both the percentage of membrane contact (juxtaposition) and the soma size of MNCs were increased in response to the rise of plasma osmolarity, and decreased to control level on the seventh day of rehydration. The number of synapses including both single synapses and multiple synapses per 100 microns soma membrane was lower than control on the fifth day of dehydration, but it was not different from controls on the tenth day of dehydration, and on the seventh and fourteenth day of rehydration. The total number of synapses per 100 microns soma membrane, the synaptic density, was maintained relatively constant, although soma size was progressively changed during dehydration or rehydration. This synaptic reorganization seems to be mainly regulated by synaptic sprouting during dehydration and by degradation of synapses during rehydration.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1993

The effect of sound level, temperature and dehydration on the brainstem auditory evoked potential in anuran amphibians.

Brainstem auditory evoked potentials (BAEPs) were used to examine the effects of sound level, temperature, and dehydration on the auditory pathway of three species of anuran amphibians: Rana pipiens, Bufo americanus and B. terrestris. BAEP latency, amplitude and a measure of threshold were determined for all stimulus and test conditions. Threshold values obtained with this technique were similar to other neural measures of threshold in anurans, and were stable for repeated measures within 12 h and over three days. Transient changes in temperature caused non-linear changes in BAEP threshold and latency. Above 20 degrees C small threshold shifts were elicited, while below 20 degrees C we observed rapid deterioration of threshold. Animals acclimated to a cold temperature (14 degrees C) were acoustically less sensitive than warm (21 degrees C) animals, even when both groups were tested at colder temperatures. Because peripheral components of the BAEP were most affected by both transient and acclimation (longer term) cooling and warming, the sensory epithelium appears to be the most temperature-sensitive component of the auditory pathway. Dehydrated frogs showed no auditory dysfunction until a critical level of dehydration was reached. More dehydration-resistant species (B. terrestris and B. americanus) were less susceptible to BAEP degradation near their critical dehydration level.

Acoustic Stimulation↗