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Investigation of the infertile couple: a one-stop outpatient endoscopy-based approach.

Transvaginal hydrolaparoscopy (THL) is a new culdoscopic technique for exploration of the pelvic cavity that takes advantage of micro-endoscopic technology and uses aquaflotation for inspection of the tubo-ovarian structures. In infertility patients, THL is systematically combined with mini-hysteroscopy, chromopertubation, fimbrioscopy and, when indicated, salpingoscopy. Mini-hysteroscopy in combination with the chromopertubation test allows accurate assessment of the uterine cavity and tubal patency. The transvaginal access combined with the aquaflotation during THL facilitates detailed inspection of the tubo-ovarian structures and detection of subtle pelvic disease. This combined transvaginal endoscopic approach allows complete evaluation of the reproductive tract. THL is better tolerated than hysterosalpingography, less invasive than standard laparoscopy, and can be used safely as a first line investigation of the female partner in a one-stop infertility clinic.

Cost-Benefit Analysis↗

Salpingoscopic findings in women undergoing sterilization.

We report the results of a prospective study carried out to investigate the appearance of the Fallopian tube lumen in 30 apparently normal women with a normal reproductive career, patient Fallopian tubes and a normal pelvic appearance at laparoscopy. The salpingoscopic appearance of the ampulla and fimbria of both Fallopian tubes was normal in 20 women. Amongst the other 10 women there were intratubal adhesions (n = 2), fronds attached to mucosal folds (n = 4), irregular blood vessels in mucosal folds (n = 2), unusual mucosal folds (n = 3) and black 'spots' within mucosal folds (n = 3). In three women who underwent salpingoscopy during the preovulatory phase of the ovarian cycle, the mucosal fold edges appeared rounded and contained blood vessels with brightly reflective walls. Our findings suggest that there is variation in the endoscopic appearance of the tubal lumen in women with a normal reproductive career. Some of these findings may be perceived to be tubal pathology causing subfertility.

Culdoscopy↗

Scintigraphic and culdoscopic diagnosis of bile peritonitis complicating liver biopsy.

After an ultrasound-guided percutaneous liver biopsy, a 37-year-old woman developed a clinical syndrome suggestive of bile peritonitis, despite the presence of nondilated bile ducts and a surgically absent gallbladder. The diagnosis was established via hepatobiliary scintigraphy, which demonstrated aberrant biliary flow, as well as by culdocentesis, which yielded bilious fluid. The patient was managed medically and recovered uneventfully; follow-up biliary scanning established resolution of bile extravasation.

Adult↗

In vivo culture of IVM/IVF embryos in bovine oviducts by transvaginal endoscopy.

This study was conducted to establish a new approach for in vivo culture of in vitro produced embryos in the bovine oviduct by transvaginal endoscopy. Embryos were in vitro matured, fertilized and cultured for 1-4 days and assigned to groups consisting of 10-30 embryos. Embryos were transferred unilaterally into oviducts of 24 heifers by the means of transvaginal endoscopy. After 3-6 days of in vivo incubation embryos were re-collected. Experiment I aimed to evaluate the capability of embryos to migrate to the uterus. The uterine horns of four animals were flushed first, followed by a combined flushing of both oviducts and uterine horns resulting in collection rates of 31 and 34%, respectively. In experiment II, the transfer of embryos into the oviduct close to ovulation (day 1-2--experiment IIA) or at a more advanced cyclic stage (day 3--experiment IIB) succeeded in the collection of 46 and 34% of the transferred complexes, of which 13 and 37% showed the blastocyst stage. This is the first report of successful recovery of transferable blastocysts by transvaginal endoscopy after tubal in vivo culture in the homologous species of originally in vitro produced embryos.

Animals↗

Hormonal contraceptives, human papillomaviruses and cervical cancer; some observations from a colposcopy clinic.

Observations from a colposcopy clinic from 1982-1985 are presented and show that in women with histologically proven precancerous lesions, those using hormone contraception had a significantly higher infection rate with human papillomavirus (HPV). Hormone contraception was preferred by younger women. Because information relating to sexual behaviour was not available for analysis, the findings in this study need to be interpreted with caution. The association of HPV and cervical cancers is well established. If hormonal contraceptive use and simultaneous HPV infection are associated with cervical neoplasia, and laboratory evidence supports this thesis, then further urgent investigations are needed for the implications are grave.

Adolescent↗