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At least 307 records · Page 17Linked to original sources
PubMed1997

A novel 90-kDa stress protein induced in fish cells by fish rhabdovirus infection.

A 90 kDa cellular protein in a fish cell, CHSE-214, showed increased expression by the infection of infectious hematopoietic necrosis virus (IHNV), heat shock, 2-mercaptoethanol, copper sulfate, and cadmium sulfate, and was detected in various kinds of cells such as human, rat, and mouse cells. The molecular mass of the 90 kDa protein was different from those of the hsp90 and grp94. In addition, all the anti-stress protein MAbs did not react with the 90 kDa protein. Finally, the subcellular distribution of the 90 kDa protein, determined by Western blots of subcellular fractions, was found to be mainly nuclear, both in normal and IHNV-infected CHSE-214 cells. The present results indicate that the 90 kDa protein is a kind of stress protein. However, based on its molecular mass, antigenic characteristics, and subcellular distribution, it is likely that this protein is a novel stress protein that has not been previously described in animal systems, especially in fish systems.

Animals↗
PubMed1998

Hsp27 phosphorylation is induced in alveolar macrophages exposed to CdO-coated silica particles.

Protein phosphorylation in bovine alveolar macrophages (BAM) activated by quartz dusts and metal oxide-coated silica particles was investigated by means of two-dimensional electrophoresis (2D-PAGE) and densitometric 32[P]-phosphate image analysis. BAM activity was monitored by determining generated superoxide anions and hydrogen peroxide. In vitro stimulation of BAM with cadmium oxide-coated silica particles (LiC-CdO) resulted in characteristic time-dependent changes in 2D-PAGE spot patterns, that were similar to the effects induced by 4ss-phorbol-12-myristate-13-acetate (PMA). Phosphorylation of two proteins with apparent molecular masses of 29 and 42 kDa appeared as main signals in both LiC-CdO and in PMA treated BAM but with different kinetics. Phosphoprotein spot pp29 was identified as an isoelectric form of Hsp27 by microsequence and Western blot analysis. In contrast to PMA stimulation, LiC-CdO-induced Hsp27 and p42 phosphorylation did not correlate with the amount of generated reactive oxygen intermediates. Other potent BAM activators like quartz dust SIKRON F600 or VO-coated silica particles did not show Hsp27 and p42 phosphorylation. LiC-CdO-mediated Hsp27 phosphorylation was inhibited by SB 203580 indicating that p38 MAP kinase is the upstream mediator of the activated signaling pathway(s), while MEK inhibitor PD 98059 had no effect.

Animals↗
PubMed1996

Accumulation and physiological and biochemical effects of cadmium in a simple aquatic food chain.

The toxicity of cadmium with regard to the vegetative reproduction of duckweed, Lemna gibba, grown in sterile culture, was determined. The EC50 was found to be 800 ppb. Duckweed grown in 2.24 ppm cadmium (supplied as cadmium nitrate) for 7 days accumulated 98.5% of the available cadmium from the growth medium. Plants that had been grown for 7 days in 2.24 ppm cadmium and control plants were fed to red swamp crayfish, Procambarus clarkii, for 14 days. The concentrations of cadmium were measured in hepatopancreata and muscles of crayfish on Day 0 and in crayfish fed duckweed grown in cadmium for 14 days. Accumulation of this metal in hepatopancreata increased 26-fold, i.e., 176.80 ppb on Day 0 to 4657.56 ppb on Day 14, and in muscles almost 7-fold, i.e., 6.75 ppb on Day 0 to 46.28 ppb on Day 14. Crayfish fed cadmium-containing duckweed demonstrated inhibition (55% after 14 days of feeding) of acetylcholinesterase activity in their central nervous tissue compared to crayfish fed cadmium-free duckweed. The ovarian index and total lipids content in the ovaries of crayfish fed cadmium-containing duckweed demonstrated significant increases on Day 14.

Animals↗
PubMed1999

Resistance of Eisenia fetida (Oligochaeta) to cadmium after long-term exposure.

The reliance on earthworms as test organisms in risk assessment studies of polluted environments raises the question whether they can evolve resistance, e.g., by adaptation to specific toxicants. Protection criteria may be biased if sensitivity data from adapted populations are used. Increased resistance to the heavy metal cadmium has not yet been determined for terrestrial Oligochaeta. Eisenia fetida was exposed to a sublethal concentration of cadmium sulfate for more than 10 generations. Clitellate worms from this culture were used in experiments to determine the extent of possible tolerance for the heavy metal. Preexposed animals as well as worms with no previous history of exposure to cadmium were exposed to a control substrate without cadmium and also to two substrates with 600 and 1200 microg g-1 cadmium. Changes in biomass, cocoon production, and hatching success were monitored. The results obtained indicated that in both substrates in which cadmium was present the preexposed worms performed better than the unexposed worms with respect to growth rate but not reproductively. In the substrate without cadmium the preexposed worms exhibited signs of poisoning after a few weeks. Preexposed and unexposed worms were also exposed to concentrations of 1500 to 4000 microg g-1 cadmium sulfate in an artisol medium for a period of 2 weeks. The preexposed worms survived higher concentrations of cadmium than the unexposed group and some specimens from the unexposed group had a gross increase in body fluids. It is concluded that worms with a long-term history of exposure to the metal developed resistance to cadmium.

Animals↗
PubMed1999

Short-term toxicological effects of cadmium on the woodlouse, Porcellio laevis (Crustacea, Isopoda).

Terrestrial isopods have been used in a number of studies as test organisms concerning the effects of chemicals in a (eco-)toxicological context. They are saprophages, which are in direct contact with polluted plant material. Cadmium is a metal with no known biological function and is toxic to life. There are a variety of anthropogenic sources of cadmium in the environment. The aims of this study were to determine the LC50 for cadmium sulfate for Porcellio laevis and the acute effect of this metal salt on the mass of this species and also to determine if P. laevis could distinguish and avoid cadmium sulfate-contaminated leaves. Acute toxicity tests and behavioral tests were conducted using concentrations ranging from 10,000 to 40,000mg/kg cadmium sulfate. The LC50 obtained of 26,700 mg/kg cadmium sulfate is an indication of P. laevis' tolerance to cadmium contamination. P. laevis also exhibited the ability to distinguish and avoid leaves contaminated with high cadmium sulfate levels.

Animals↗
PubMed1997

Regulation of promoter-CAT stress genes in HepG2 cells by suspensions of particles from ambient air.

A panel of HepG2-derived cell lines (CAT-Tox [L] assay, Xenometrix), harboring stress genes consisting of a sequence for chloramphenicol acetyltransferase (CAT) under the transcriptional regulation from mammalian promoters and response elements, was exposed for 18-24 hr to aqueous suspensions of urban dusts (SRM-1648, SRM-1649, EHC-93) or PM2.5 particles (particulate matter < 2.5 micron). Expression of CAT protein was measured by enzyme-linked immunosorbent assay. Induction of the CAT genes was verified with benzo[a]pyrene (CYP1A1, cytochrome P450 1A1 promoter; GSTYa, glutathione transferase subunit Ya promoter; XRE, xenobiotic response element), cadmium sulfate, and copper sulfate (HMTIIa, metallothionein IIa promoter; HSP70, heat shock protein 70 promoter). The urban dust suspensions were active on CYP1A1, GSTYa, and XRE cell lines. SRM-1648 and SRM-1649 were twice as potent as EHC-93 per unit mass in inducing the xenobiotic-dependent responses, which correlated with contents in polycyclic aromatic hydrocarbons. These three reference particles, as well as six PM2.5 preparations collected on hi-vol filters in the Great Lakes basin, were also found to induce HMTIIa and HSP70, the magnitude of the responses correlating closely with the amount of soluble copper in the particulate preparations. The results indicate that bioavailable chemical species in the unfractionated particles can directly and quantitatively induce xenobiotic, metal, and stress-dependent responses in a target cell model, resulting in patterns of gene induction consistent with the chemical compositions of the environmental materials. We propose that cell culture models could be helpful for toxicodynamic inferences in adjunct to environmental monitoring and exposure assessments.

Air Pollutants↗
PubMed2002

The sorption of divalent metal ions on AlPO4.

The sorption of Cu(2+), Pb(2+), Ni(2+), and Cd(2+) ions on the aluminum(III) phosphate was observed to increase with increases in the concentration, temperature, and pH of the system. The apparent dissociation (pK(a)), binding (pK(b)) and exchange (pK(ex)) constants of aluminum(III) phosphate were evaluated and found to be dependent upon the temperature and nature of the metal cations. The values of the dissociation constants (pK(a)) followed the order Pb(2+)<Cu(2+)<Cd(2+)<Ni(2+)<K(+), opposite to the values of the binding constants and selectivity shown by the solid. Ion exchange between protons from the surface and metal cations from aqueous solution was found to be responsible for the metal ions sorption by AlPO(4). The values of the dissociation (pK(a)) and exchange (pK(ex)) constants were used to estimate the corresponding apparent thermodynamic parameters (DeltaH(o)(diss), DeltaS(o)(diss) and DeltaH(o)(exch), DeltaS(o)(exch)). The values of DeltaH(o)(diss) and DeltaH(o)(exch) were found to be positive, which showed that both the dissociation and exchange processes were endothermic in nature. The DeltaS(o)(diss) and DeltaS(o)(exch) were also found to be positive, which showed that both the deprotonation and exchange processes on the AlPO(4) were entropy driven and spontaneous.

Adsorption↗
PubMed1998

Action potential prolongation and potassium currents in left-ventricular myocytes isolated from hypertrophied rabbit hearts.

We have studied the action potential characteristics and potassium currents in single left ventricular myocytes isolated from control and hypertrophied rabbit hearts. Left-ventricular hypertrophy (LVH) was induced following perinephritis-induced hypertension. Control animals underwent a sham operation. Animals were killed at 10 weeks post-operation. Left-ventricular myocytes were isolated by an enzyme dissociation technique. Action potential duration (APD) at 50 and 90% repolarisation was prolonged in myocytes obtained from hypertrophied compared to control hearts over the range of stimulation frequencies (0.1-1.5 Hz). This prolongation in APD was more pronounced in epicardial compared to endocardial myocytes. Steady-state ionic current, measured at the end of voltage clamp steps of 3-s duration, stepping at intervals of 10 mV, from a holding potential of -40 mV, was similar in control and hypertrophied myocytes. However, when normalised for capacitative cell surface area, steady-state current was significantly smaller in hypertrophied myocytes over the voltage range -40 to -60 mV and at potentials greater than +10 mV. Inward rectifier potassium current (IKl), identified as the barium chloride (0.1 mM)-sensitive current, contributed to the steady state current at negative potentials. Normalised IKl was significantly smaller in hypertrophied compared to control myocytes at potentials negative to -60 mV. Peak transient outward potassium current (Ito) density was reduced in hypertrophied compared to control myocytes at 0 and +10 mV, from a holding potential -80 mV (12.9 +/- 2.3 v 24.9 +/- 3.9 microA cm2, at +10 mV, P < 0.05). Steady-state inactivation of Ito was similar in control and hypertrophied myocytes. In conclusion, LVH induced by perinephritis hypertension in the rabbit is associated with a prolongation in APD. Reductions in IKl, sustained outward current and Ito may contribute to the prolongation in APD.

Action Potentials↗
PubMed1997

Comparison of inflammatory lung responses in Wistar rats and C57 and DBA mice following acute exposure to cadmium oxide fumes.

Inhalation of cadmium oxide (CdO) is a significant form of human exposure to cadmium (Cd). Furthermore, there is epidemiological and experimental data relating Cd inhalation with lung cancer. Animal studies indicate that rats are more susceptible to Cd-induced lung cancer than mice, but interstrain sensitivity differences to Cd-induced pulmonary inflammation or carcinogenesis have not been addressed in either species. We compared pulmonary inflammatory processes in Wistar Furth (WF) rats with those in C57 and DBA mice exposed to freshly generated CdO fumes in nose-only inhalation chambers. Animals were exposed to 1 mg Cd/m3 for 3 hr and terminated immediately or 1, 3, and 5 days after exposure. Control animals were exposed to air/argon furnace gases. Cd-induced lung injury was assessed by bronchoalveolar lavage fluid (BALF) analyses, histopathology, and immunohistochemical detection of cell proliferation. Inhalation of CdO resulted in pulmonary inflammatory processes that varied widely across species and strains. C57 mice responded with faster and greater influx of neutrophils and proliferation of alveolar macrophages, type II epithelial cells, and bronchiolar epithelial cells compared to DBA mice or WF rats. DBA mice retained a greater percentage of inhaled Cd in the lungs and presented higher levels of BALF protein than C57 mice or rats. In comparison to mice, WF rats responded with a more transient inflammatory response in BALF parameters and higher degree of acute inflammation in lung tissue. The more pronounced proliferation of alveolar and bronchiolar epithelial cells observed in C57 mice might indicate higher susceptibility of this mice strain to Cd-induced lung carcinogenesis compared to DBA mice or WF rats. Furthermore, the present results of fewer inflammatory cells and lower proliferation of epithelial cells in DBA mice in association with our previous observation of higher Cd-induced metallothionein protein in this strain suggest that DBA might be less susceptible to the pulmonary carcinogenic effects of inhaled Cd than C57 mice or WF rats. We conclude that mice might not necessarily be more resistant than rats to the carcinogenic effects of inhaled Cd, since intraspecies susceptibility differences are strongly suggested by the present data. An extrapolation of this conclusion is that genetic variations in the human population may determine individual sensitivity differences to inhaled Cd.

Administration, Inhalation↗
PubMed1985

Effect of some heavy metal salts on hepatic monooxygenases after subchronic exposure.

The effects of subchronic exposure of some heavy metal salts on the activity of the rat liver monooxygenases and NADPH-dependent lipid peroxidation have been studied. The salts of Co, Cd and Zn (when given repeatedly for one month in subtoxic doses) shortened the duration of hexobarbital sleeping time, increased the activities of Ethylmorphine-N-demethylase (EMD) and Benzphetamine-N-demethylase (BND), and the cytochrome P-450 and heme contents. The same metal salts increased the activity of delta-aminolevulinic acid synthetase (ALAS), decreased that of Heme-oxygenase and decreased NADPH-dependent lipid peroxidation. Whereas the salts of Co, Cd and Zn seem to exert an enzyme-inducing action on the mixed function oxidizing enzyme systems, the salts of Hg and Ni did not show such effects.

5-Aminolevulinate Synthetase↗
PubMed1996

Increase in number of Weibel-Palade bodies and endothelin-1 release from endothelial cells in the cadmium-treated rat thoracic aorta.

Male rats received daily intraperitoneal injections of cadmium sulphate (2.0 mg/kg) for 3 (Cd-3 group), 6 (Cd-6 group) and 8 days (Cd-8 group). The blood samples were prepared for endothelin (ET)-1 assay, and the thoracic aorta was investigated by both electron microscopy and immunoelectron microscopy using anti ET-1 sera. The plasma ET-1 concentrations of both Cd-6 and Cd-8 groups increased significantly in a cumulative dose-dependent manner. The cadmium-treated rat aorta showed an increase in the number of Weibel-Palade (WP) bodies in endothelial cells, and degranulation and exocytosis of WP bodies occurred exclusively in the Cd-8 group. Immunoreaction for ET-1 was localized preferentially in WP bodies of both cadmium-treated and control groups, and in the rough endoplasmic reticulum of the cadmium-treated groups only. Reactivity was also found on the WP bodies undergoing exocytosis in the Cd-8 group. Cadmium intoxication induces an increase in number of ET-1-storing WP bodies in the rat aorta endothelium. The enhancement of extracellular release of their contents by exocytosis results in elevation of the plasma ET-1 concentration.

Animals↗
PubMed1992

Effect of photocorrosion on cadmium sulfide suspensions applied in animal inhalation studies with CdS particles.

Cadmium sulfide aerosol (90 micrograms Cd/m3) was generated from an aqueous suspension for 12 weeks and the ratio of Cd++/Cdtotal in the suspension and the resulting aerosol was determined. After 4 weeks the concentration of Cd++ in the suspension and the aerosol leveled off at 43.5 +/- 11.4% and 35.8 +/- 6.8%, respectively. Therefore, lung tumors in rats observed after inhalation exposure to such CdS aerosols (Glaser et al. 1990) may have been caused not only by the effect of CdS but also by the influence of inhaled Cd++ or CdSO4. The contribution of CdS particles to the observed lung tumor rate depends on the bioavailability of CdS retained in the lungs. The photochemical decay of CdS in aqueous suspensions only occurred when exposed to light and adjusting the suspension up to pH 3 did not increase the decomposition rate of CdS. Ethanolic suspensions of CdS are much more stable than aqueous ones and experiments with suspensions kept under shield gases showed that the decay of CdS is not dependent on oxygen.

Administration, Inhalation↗
PubMed1982

Evaluation of a miniature CdTe detector for monitoring left ventricular function.

A miniature CdTe probe interfaced to a microcomputer was used to measure left ventricular ejection fraction (LVEF) in 25 patients. LVEF obtained with the CdTe module, in the beat-to-beat mode, or the integrated gated mode agreed well with LVEF obtained with a gamma camera (r = 0.80; r = 0.82 respectively). Similarly, LVEF by CdTe probe agreed with LVEF obtained by gated equilibrium studies performed with a computerized NaI probe. The CdTe probe can provide comparable measurement of LVEF at a fraction of the cost of a camera-computer system and, being small and lightweight, the CdTe probe is adaptable for monitoring patients in intensive care facilities.

Adult↗
PubMed1995

Energy-dispersive X-ray analysis of the extracellular cadmium sulfide crystallites of Klebsiella aerogenes.

Klebsiella aerogenes forms electron-dense particles on the cell surface in response to the presence of cadmium ions in the growth medium. These particles ranged from 20 to 200 nm in size, and quantitative energy dispersive X-ray analysis established that they comprise cadmium and sulfur in a 1:1 ratio. This observation leads to the conclusion that the particles are cadmium sulfide crystallites. A combination of atomic absorption spectroscopy, inductively coupled plasma mass spectrometry, and acid-labile sulfide analysis revealed that the total intracellular and bound extracellular cadmium:sulfur ratio is also 1:1, which suggests tha the bulk of the cadmium is fixed as extracellular cadmium sulfide. The tolerance of K. aerogenes to cadmium ions and the formation of the cadmium sulfide crystallites were dependent on the buffer composition of the growth medium. The addition of cadmium ions to phosphate-buffered media resulted in cadmium phosphate precipitates that remove the potentially toxic cadmium ions from the growth medium. Electron-dense particles formed on the surfaces of bacteria grown under these conditions were a combination of cadmium sulfide and cadmium phosphates. The specific bacterial growth rate in the exponential phase of batch cultures was not affected by up to 2mM cadmium in Tricine-buffered medium, but formation of cadmium sulfide crystallites was maximal during the stationary phase of batch culture. Cadmium tolerance was much lower (10 to 150 microM) in growth media buffered with Tris, Bistris propane, Bes, Tes, or Hepes. These results illustrate the importance of considering medium composition when comparing levels of bacterial cadmium tolerance.

Cadmium↗