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Trace element analysis of Bronze Age skeletal and crematory graves from southern Poland for diet reconstruction.

The basic aim of the present study was to determine the diet of human populations dating from the Bronze Age and the time of Roman influence. The osseous material under examination came from skeletal and crematory burials of the Lusatian and Przeworks Cultures, found in a cemetery at Opatów, Klobuck District, Silesian Province. Three elements: strontium, zinc and calcium were chosen as basic diet determinants. The three elements and the proportions between them are the most frequently used factors that permit a description of the relative proportion of animal and plant protein in a diet. It was assumed that the differentiation of burial ritual was paired with a diverse mode and quality of nourishment. Interdisciplinary osteological analyses, based on physicochemical studies of the odontologic material in the context of archaeological data (culture affiliation, type of burial, grave furnishings), permit a complex analysis of the issues connected with the biology of human groups, their demographic structure or, eventually, paleostratigraphy of primeval communities. It has been found that the type of burial and the richness of grave furnishings are most closely connected with the ultimate differentiation of Zn and Ca concentrations and the value of the Sr/Zn ratio. Because the richly furnished graves are at the same time mostly skeletal burials, it cannot be unequivocally stated which of the above-mentioned factors is of paramount importance. It has also been demonstrated that representatives of the Przeworsk Culture, chronologically younger than the Lusatian one that inhabited the same geographic region, show a lower Zn concentration and a higher Sr/Zn ratio.

Adult↗

The changing window of conditions that promotes germination of two fire ephemerals, Actinotus leucocephalus (Apiaceae) and Tersonia cyathiflora (Gyrostemonaceae).

BACKGROUND AND AIMS: Following a period of burial, more Actinotus leucocephalus (Apiaceae) and Tersonia cyathiflora (Gyrostemonaceae) seeds germinate in smoke water. The main aim of this study was to determine whether these fire-ephemeral seeds exhibit annual dormancy cycling during burial. This study also aimed to determine the effect of dormancy alleviation on the range of light and temperature conditions at which seeds germinate, and the possible factors driving changes in seed dormancy during burial. METHODS: Seeds were collected in summer, buried in soil in mesh bags in autumn and exhumed every 6 months for 24 months. Germination of exhumed and laboratory-stored (15 degrees C) seeds was assessed at 20 degrees C in water or smoke water. Germination response to light or dark conditions, incubation temperature (10, 15, 20, 25 and 30 degrees C), nitrate and gibberellic acid were also examined following burial or laboratory storage for 24 months. In the laboratory seeds were also stored at various temperatures (5, 15, 37 and 20/50 degrees C) for 1, 2 and 3 months followed by germination testing in water or smoke water. KEY RESULTS: The two species exhibited dormancy cycling during soil burial, producing low levels of germination in response to smoke water when exhumed in spring and high levels of germination in autumn. In autumn, seeds germinated in both light and dark and at a broader range of temperatures than did laboratory-stored seeds, and some Actinotus leucocephalus seeds also germinated in water alone. Dormancy release of Actinotus leucocephalus was slow during dry storage at 15 degrees C and more rapid at higher temperatures (37 and 20/50 degrees C); weekly wet/dry cycles further accelerated the rate of dormancy release. Cold stratification (5 degrees C) induced secondary dormancy. By contrast, no Tersonia cyathiflora seeds germinated following any of the laboratory storage treatments. CONCLUSIONS: Temperature and moisture influence dormancy cycling in Actinotus leucocephalus seeds. These factors alone did not simulate dormancy cycling of Tersonia cyathiflora seeds under the conditions tested.

Analysis of Variance↗

Mortality impact of AIDS in Addis Ababa, Ethiopia.

OBJECTIVE: To measure the impact of HIV on mortality in Addis Ababa, Ethiopia. DESIGN: A retrospective review of burials at three cemeteries, 1987-2001 and a prospective surveillance of burials at all (n = 70) cemeteries, February-May, 2001. METHODS: The age, sex, and date of burial were recorded; in the absence of denominators, we compared the ratio of deaths of persons 25-49 versus 5-14 years of age per calendar year, using logistic regression, adjusting for sex and site. The age- and sex- specific mortality were calculated and compared with pre-HIV mortality in 1984. RESULTS: Of 17,519 deaths, retrospectively reviewed, complete data were available for 6342 (47%) females and 7269 (53%) males. During 1987-2001, the '25-49' versus '5-14' group all-cause mortality ratio increased by 8.5% per calendar year (P < 0.05). A total of 5101 deaths were recorded in the prospective surveillance. Crude mortality rates were 9.5/1000 per year (men) and 7.1/1000 per year (women). In comparison with 1984, 5.0-times as many men and 5.3-times as many women died in the age group 35-39 years. Attributing the increase in mortality in ages 15-60 to HIV in the period 1984-2001, Ethiopian men and women have a probability of 18.8 and 17.8%, respectively, of dying of HIV before age 60. CONCLUSION: Burials increased significantly among the '25-49', versus the '5-14' group, during the period 1987-2001. This trend, and a five-times higher mortality in 2001 than in 1984 in those aged 35-39 years demonstrate a severe impact of HIV on mortality. Continuing surveillance of burials is recommended.

Acquired Immunodeficiency Syndrome↗

Mortality impact of AIDS in Addis Ababa, Ethiopia.

OBJECTIVE: To measure the impact of HIV on mortality in Addis Ababa, Ethiopia. DESIGN: A retrospective review of burials at three cemeteries, 1987-2001 and a prospective surveillance of burials at all (n = 70) cemeteries, February-May, 2001. METHODS: The age, sex, and date of burial were recorded; in the absence of denominators, we compared the ratio of deaths of persons 25-49 versus 5-14 years of age per calendar year, using logistic regression, adjusting for sex and site. The age- and sex-specific mortality were calculated and compared with pre-HIV mortality in 1984. RESULTS: Of 17,519 deaths, retrospectively reviewed, complete data were available for 6342 (47%) females and 7269 (53%) males. During 1987-2001, the '25-49' versus '5-14' group all-cause mortality ratio increased by 8.5% per calendar year (P < 0.05). A total of 5101 deaths were recorded in the prospective surveillance. Crude mortality rates were 9.5/1000 per year (men) and 7.1/1000 per year (women). In comparison with 1984, 5.0-times as many men and 5.3-times as many women died in the age group 35-39 years. Attributing the increase in mortality in ages 15-60 to HIV in the period 1984-2001, Ethiopian men and women have a probability of 18.8 and 17.8%, respectively, of dying of HIV before age 60. CONCLUSION: Burials increased significantly among the '25-49', versus the '5-14' group, during the period 1987-2001. This trend, and a five-times higher mortality in 2001 than in 1984 in those aged 35-39 years demonstrate a severe impact of HIV on mortality. Continuing surveillance of burials is recommended.

Acquired Immunodeficiency Syndrome↗

[Effects of sand-burying on seed germination and seedling emergence of six psammophytes species].

This paper studied the effects of sand-burying on the seed germination and seedling emergence of six dominance plants in Keerqin Sandy Land. Six treatments of artificially sand-burying to depths 0, 2, 4, 6, 8, 10 and 12 cm were designed. The results showed that the seed germination of Agriophyllum squarrosum was significantly different (P < 0.05) between different treatments, but that of Artemesia halodendron was only significantly different (P< 0.05) between 2 cm and other burial depths. For other four plants, their seed germination was significantly different (P < 0.05) only between 0 cm and other burial depths. Sand-burying significantly affected the seedling emergence of all plant species. The emergence rate decreased with increasing burial depth. Higher emergence percentages were obtained at 2 and 4 cm burial depths. The species with larger seeds emerged from deeper depth, and the maximum burial depth of seedling emergence was Sophora flavescdens > Atraphaxis manshurica > or = Agriophyllum squarrosum > Hedysarum fruticosum > Bassia dasyphylla > Artemesia halodendron.

Chenopodiaceae↗

A critical review of the German Paleolithic hominin record.

We review the hominin fossil record from western Central Europe in light of the recent major revisions of the geochronological context. The mandible from Mauer (Homo heidelbergensis), dated to circa 500,000 years ago, continues to represent the earliest German hominin and may coincide with the occupation of Europe north of the high alpine mountain chains. Only limited new evidence is available for the Middle Pleistocene, mostly in the form of skull fragments, a pattern that may relate to taphonomic processes. These finds and their ages suggest the gradual evolution of a suite of Neandertal features during this period. Despite new finds of classic Neandertals, there is no clear proof for Neandertal burial from Germany. Alternatively, cut marks on a skull fragment from the Neandertal type site suggest special treatment of that individual. New Accelerator Mass Spectrometry (AMS) radiocarbon dates of previous finds leave little reliably dated evidence for anatomically modern humans (AMH) in Europe before 30,000 BP; the remains from Hahnöfersand, Binshof-Speyer, Paderborn-Sande, and Vogelherd are now of Holocene age. Thus, a correlation of AMH with the Aurignacian remains to be proven, and the general idea of a long coexistence of Neandertals and AMH in Europe may be questioned. In western Central Europe, evidence of Gravettian human fossils is also very limited, although a new double grave from lower Austria may be relevant. The only dated burial from the German Upper Paleolithic (from Mittlere Klause) falls into a time period (circa 18,600 BP) represented by only a few occupation sites in western Central Europe. A number of human remains at Magdalenian sites appear to result from variable (secondary) burial practices. In contrast, the Final Paleolithic (circa 12,000-9600 cal. BC) yields an increase of hominin finds, including multiple burials (Bonn-Oberkassel, Neuwied-Irlich), similar to the situation in western and southern Europe.

Animals↗

Decomposition of buried bodies and methods that may aid in their location.

This is the second report on an ongoing study conducted to collect data on the decompositional rates of human cadavers and the first on buried cadavers. Six unembalmed human cadavers were buried separately in unlined trenches of various depths and allowed to naturally decompose for a time period ranging from a month to a year. During the period of burial, data were collected daily on the air, soil, and cadaver temperature at each burial site. At the end of each specified burial period the cadavers were exhumed and examined for the degree of decomposition which had taken place as well as changes in the soil pH, surface vegetation, and carrion insect activity. Analysis of the data shows that the decomposition rate of buried cadavers is highly dependent on the depth of burial and environmental temperatures. The depth at which the cadaver was buried also directly affected the degree of soil and vegetational changes as well as access by carrion insects. Application of this information can contribute to a more accurate estimation of time since death of a buried corpse and may aid in the location of such corpses.

Adult↗

Fijian cannibalism: osteological evidence from Navatu.

The hypothesis that the human remains from the Navatu midden (50 BC to AD 1900) in Fiji represent cannibalized individuals was tested by an analysis of the skeletal remains. The site includes formal human burials and a separate, contemporaneous midden containing commingled fragmentary human and nonhuman bones. All remains were examined for a variety of modifications. The medium mammal and human remains in the midden have similar modifications: ancient breaks (92% of midden human specimens and 88% of medium mammal specimens), burning (29% and 11%), crushing (1% and 1%), cutmarks (9% and 8%), peeling (4% and 1%), and percussion pits (1% and 1%). The human burials (for which cannibalism had not been hypothesized) are essentially unmodified except for some breakage. The pattern of element representation and low incidence of animal bitemarks rules out carnivores and rodents as major modifiers of the assemblage. The breakage patterns, element representation, light weathering, and rarity of random striae indicate that sediment pressure, wave action, weathering, and trampling did not significantly alter the remains. The modifications of the midden human and nonhuman remains generally correspond in type and frequency. The evaluation of the assemblage's taphonomic history suggests that most of the modifications were caused by humans. The Navatu midden human sample does not resemble assemblages interpreted as secondary burials with defleshing, nor does it resemble violence-derived assemblages. The burials at Navatu and other Fijian sites indicate that the various noncannibalistic Fijian mortuary rituals do not mimic butchery and consumption. Therefore, the hypothesis of cannibalism at Navatu is supported.

Bone and Bones↗

Differential skeletal preservation at Windover Pond: causes and consequences.

In this paper, we evaluate the causes of differential skeletal preservation in the Windover Pond skeletal series (8BR246). We collected data on sex and age for approximately 110 individuals, and calculated a preservation score for each individual based on the presence of 80 skeletal landmarks. Our research questions evaluated the relationship between bone preservation and individual age and sex, and between the presence of preserved brain material and skeletal preservation, and the effects of burial location on bone preservation. The results indicate variability in average preservation for the sample (micro = 0.53, SD = 0.22) with an apparent lack of sex-specific (P = 0.79) or age-specific (P = 0.37) differences in preservation. The relationship between brain and skeletal preservation (P = 0.15) was not significant. The horizontal distribution of burials was not significantly correlated with skeletal preservation (north: r = -0.10, P = 0.93; east: r = 0.09, P = 0.45); however, vertical depth was a significant predictor of preservation (r = -0.31, P = 0.005), indicating that skeletal preservation decreased as burials were located closer to the ground surface. The observed variability in preservation scores may be related to the partial drying and resubmergence of the uppermost burials for the last few millennia. Comparison of Windover element-specific survival rates with previous analyses based on terrestrial samples (Galloway et al. [1997] Forensic taphonomy, Boca Raton: CRC Press; Waldron [1987] Death, decay and reconstruction, Manchester: Manchester University Press; Willey et al. [1997] Am J Phys Anthropol 104:513-528) affirms the relationship between element weight or density and bone survival. The unique taphonomic context of our study sample effected little change in bone deterioration processes.

Age Factors↗

Alpha-helix stabilization by alanine relative to glycine: roles of polar and apolar solvent exposures and of backbone entropy.

The energetics of alpha-helix formation are fairly well understood and the helix content of a given amino acid sequence can be calculated with reasonable accuracy from helix-coil transition theories that assign to the different residues specific effects on helix stability. In internal helical positions, alanine is regarded as the most stabilizing residue, whereas glycine, after proline, is the more destabilizing. The difference in stabilization afforded by alanine and glycine has been explained by invoking various physical reasons, including the hydrophobic effect and the entropy of folding. Herein, the contribution of these two effects and that of hydrophilic area burial is evaluated by analyzing Ala and Gly mutants implemented in three helices of apoflavodoxin. These data, combined with available data for similar mutations in other proteins (22 Ala/Gly mutations in alpha-helices have been considered), allow estimation of the difference in backbone entropy between alanine and glycine and evaluation of its contribution and that of apolar and polar area burial to the helical stabilization typically associated to Gly-->Ala substitutions. Alanine consistently stabilizes the helical conformation relative to glycine because it buries more apolar area upon folding and because its backbone entropy is lower. However, the relative contribution of polar area burial (which is shown to be destabilizing) and of backbone entropy critically depends on the approximation used to model the structure of the denatured state. In this respect, the excised-peptide model of the unfolded state, proposed by Creamer and coworkers (1995), predicts a major contribution of polar area burial, which is in good agreement with recent quantitations of the relative enthalpic contribution of Ala and Gly residues to alpha-helix formation.

Alanine↗

Primary folding dynamics of sperm whale apomyoglobin: core formation.

The structure, thermodynamics, and kinetics of heat-induced unfolding of sperm whale apomyoglobin core formation have been studied. The most rudimentary core is formed at pH(*) 3.0 and up to 60 mM NaCl. Steady state for ultraviolet circular dichroism and fluorescence melting studies indicate that the core in this acid-destabilized state consists of a heterogeneous composition of structures of approximately 26 residues, two-thirds of the number involved for horse heart apomyoglobin under these conditions. Fluorescence temperature-jump relaxation studies show that there is only one process involved in Trp burial. This occurs in 20 micro s for a 7 degrees jump to 52 degrees C, which is close to the limits placed by diffusion on folding reactions. However, infrared temperature jump studies monitoring native helix burial are biexponential with times of 5 micro s and 56 micro s for a similar temperature jump. Both fluorescence and infrared fast phases are energetically favorable but the slow infrared absorbance phase is highly temperature-dependent, indicating a substantial enthalpic barrier for this process. The kinetics are best understood by a multiple-pathway kinetics model. The rapid phases likely represent direct burial of one or both of the Trp residues and parts of the G- and H-helices. We attribute the slow phase to burial and subsequent rearrangement of a misformed core or to a collapse having a high energy barrier wherein both Trps are solvent-exposed.

Animals↗

Energetic evidence for formation of a pH-dependent hydrophobic cluster in the denatured state of Thermus thermophilus ribonuclease H.

NMR studies on the denatured states of proteins indicate that residual structure often resides predominantly in hydrophobic clusters. Such hydrophobic cluster formation implies burial of apolar surface and, consequently, is expected to cause a decrease in heat capacity. We report here that, in the case of ribonuclease H from the thermophile Thermus thermophilus, a sharp decrease in denatured-state heat capacity occurs at about pH 3.8; this result points to the formation of hydrophobic clusters triggered by the protonation of several (about four) carboxylic acid groups, and indicates that the burial of apolar surface is favored by the less hydrophilic character of the uncharged forms of Asp and Glu side-chains. The process is not accompanied by large changes in optically active structure, but appears to be highly cooperative, as indicated by the sharpness of the pH-induced transition in the heat capacity. This acid-induced hydrophobic burial in denatured T.thermophilus ribonuclease H is clearly reflected in the pH dependence of the denaturation temperature (i.e. an abrupt change of slope at about pH 3.8 is seen in the plot of denaturation temperature versus pH), supporting a role for such denatured-state hydrophobic clusters in protein stability. The finding of cooperative protonation of several groups coupled to surface burial in denatured T.thermophilus ribonuclease H emphasizes the potential complexity of denatured-state electrostatics and advises caution when attempting to predict denatured-state properties on the basis of simple electrostatic models. Finally, our results suggest a higher propensity for hydrophobic cluster formation in the denatured state of T.thermophilus ribonuclease H as compared with that of its mesophilic counterpart from Escherichia coli.

Anilino Naphthalenesulfonates↗

Global accounting of PCBs in the continental shelf sediments.

The recycling longevity of hydrophobic organic contaminants (HOCs) within the global environment is set by their permanent removal through processes such as degradation and burial in geological reservoirs. More than 90% of the global sediment burial of organic carbon (OC) occurs on the continental shelves, representing < 10% of the earth's ocean area. The propensity of HOCs to associate with organic matter, and the proximity of most population centers and thus presumed source areas to coastal regions, led us to investigate shelf sediments as a depository of significance for global HOC budgets. Here, the global inventory and burial fluxes of polychlorinated biphenyls (PCBs) in continental shelf sediments were estimated on a congener-specific basis from a database of 4214 distinct continental shelf surface sediment samples. To account for near-urban sampling bias, the locations of each datum relative to nearest population center were classified as Local (< 1 km), Regional (1-10 km), or Remote (> 10 km), according to a comprehensive vector map of the world (Digital Chart of the World) in a GIS environment. The global inventory of, for instance, PCB congener 153 was 1200 ton (95% confidence limit maximum: 2100 ton; and minimum 720 ton). The Remote sub-basin of the North Atlantic contains approximately half of the global shelf sediment inventory for most of the PCB congeners studied. The shelf sediment inventories of individual PCB congeners constitute significant fractions of their recently updated cumulative historical global emissions estimates. The estimated inventory in the shelf corresponds to about 10% of maximum emission estimates for lower-chlorinated congeners. However, for the more bioaccumulable, higher-chlorinated, congeners the shelf reservoirs appear to account for up to 80% of the estimated maximum cumulative global emissions. These shelf inventories represent 1-6% of the global industrial production of PCBs. The global burial fluxes were estimated to be on the order of 8-24 ton/yr each for the eight major congeners investigated, again, with the shelf constituting a more significant removal sink of the more chlorinated congeners. The permanent removal into deeper shelf sediments of PCB153 and PCB180 suggests that the global environmental mean residence times of these pollutants are on the order of 110 and 70 years, respectively. Hence, even after production and direct releases have been halted, we may expect to be exposed to such persistent pollutants for decades and centuries to come.

Environmental Exposure↗

Measurement of helium isotopes in soil gas as an indicator of tritium groundwater contamination.

The focus of this study was to define the shape and extent of tritium groundwater contamination emanating from a legacy burial ground and to identify vadose zone sources of tritium using helium isotopes (3He and 4He) in soil gas. Helium isotopes were measured in soil-gas samples collected from 70 sampling points around the perimeter and downgradient of a burial ground that contains buried radioactive solid waste. The soil-gas samples were analyzed for helium isotopes using rare gas mass spectrometry. 3He/4He ratios, reported as normalized to the air ratio (RA), were used to locate the tritium groundwater plume emanating from the burial ground. The 3He (excess) suggested that the general location of the tritium source is within the burial ground. This study clearly demonstrated the efficacy of the 3He method for application to similar sites elsewhere within the DOE weapons complex.

Environment↗

Graveyard gleanings: socio-economic, geographical and gender inequalities in health at Tynemouth, UK, 1833-1853.

BACKGROUND: Inequalities in the health of different sections of populations are well recognized but were difficult to demonstrate before death registration was introduced in 1837. In the early years of civil registration, geographical and sex differences in mortality were clearly recognized, as were occupational hazards, but socio-economic differences were barely explored in the Annual Reports of the Registrar General. Tynemouth General Cemetery (TGC) was established in 1833 as a private cemetery with unusually detailed records. METHODS: A total of 2610 records from 1833 to 1853 were analysed. Variables used included sex, dates of death and burial, age at death, depth of burial, cause of death, place of residence and occupation. As no denominator population is available, median age at death has been used for comparisons. RESULTS: Depth of burial relates well to a hierarchy of specific occupations and so is used as a marker for socio-economic status. The median age for the burials was 12 years. People of higher socio-economic status survived longer. The people of North Shields, and especially the males, died younger than those from surrounding areas. Males outnumbered females in most age groups. CONCLUSION: Socio-economic, geographical and gender inequalities in mortality are clearly demonstrable in the early nineteenth century, without the use of registration data.

Age Distribution↗

Managing 'civilian deaths due to war operations': Yorkshire experiences during world war II.

Air raid precautions prior to and during the Second World War included guidance on the burial of civilians who had been killed by enemy bombardment. The Ministry of Health Circular 1779 was published before the start of the war, on the understanding that 'scheme-making' authorities would need time to prepare for the interment of mass fatalities. As the Blitz got underway, tensions emerged between the state's pragmatic concerns to restrict the cost and resources afforded to burial, and the wish of many local authorities to secure decent burial for their citizens. Key issues on which there was disagreement included the state-proposed use of shrouds instead of coffins, and mass interment. These practices carried overtones of the much-hated pauper funeral and ran counter to the political imperative to present civilian death as 'heroic'. This local study is placed in the wider context of the history of death and burial, and provides an indicator of the strength of attachment to customary funerary ritual during the middle of the twentieth century.

Death↗

Continental-pelagic carbonate partitioning and the global carbonate-silicate cycle.

A carbonate-silicate geochemical cycle model is developed and used to explore dynamic and climatic consequences of constraints on shallow-water carbonate burial and possible carbon loss to the mantle associated with sea-floor subduction. The model partitions carbonate deposition between shallow-water and deep-water environments and includes carbon fluxes between the mantle and lithosphere. When total lithospheric carbonate mass is constant, there are two stable steady states, one in which the carbonate burial flux is mostly continental and another in which it is mostly pelagic. The continental steady state is characterized by a low metamorphic CO2 flux to the atmosphere and predominantly shallow-water carbonate burial. The pelagic steady state is characterized by a high metamorphic CO2 flux and predominantly deep-water carbonate burial. For reasonable parameter values, when total lithospheric carbonate mass is allowed to vary, the model oscillates between predominantly continental and predominantly pelagic modes. Model results suggest that carbonate deposition patterns established during the Cenozoic may be pushing the Earth system from the continental to the pelagic mode on a time scale of 10(8) yr, with a possible consequent order-of-magnitude increase in the metamorphic CO2 flux to the atmosphere.

Carbon Dioxide↗

Time-of-flight secondary ion mass spectrometry analysis of hair from archaeological remains.

Hair from four individuals excavated from burial sites in Pacatnamu, Peru from the Moche (450-800 AD) and Lambayeque (900-1100 AD) periods was sectioned longitudinally and analysed with time-of-flight secondary ion mass spectrometry (ToF-SIMS). An attempt was made to distinguish biogenic and diagenetic contributions to the elemental concentrations in the hair samples. Significant contamination was observed to have penetrated the hair samples from the burial environment. Results from the analyses indicate that the burial environment plays an important role in the postmortem variation in elemental content of hair samples. Various elements demonstrated an ability to permeate through the hair matrix over time. In addition, NaCl and what are believed to be aluminosilicates and mineral sulphates, were observed to have accumulated on the surface of the samples. Degradation of the samples was also suspected due to the presence of molecular fragments, possibly resulting from oxidation of the keratin proteins. The results should assist in the identification of reliable elemental signals in the analysis of ancient hair samples and promote caution when considering elements that are abundant in the burial environment.

Archaeology↗