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[Preliminary study of the antagonistic effects between fosfomycin and beta-lactams on Pseudomonas aeruginosa observed on the antibiogram].

Antagonism patterns between beta-lactams (BL) and fosfomycin (FOS) were observed in antibiotic assay by agar diffusion method against the most of Pseudomonas aeruginosa strains. This antagonism was confirmed by antibiotic assays made on Mueller-Hinton agar containing subinhibiting concentrations of FOS. FOS + BL combinations, studied on the strain of Pseudomonas aeruginosa ATCC 27853 by means of checker board method, exhibit antagonism of FOS concentrations below 1 mg/l on ceftriaxone, piperacillin, ticarcillin and, less strongly, on imipenem. These results suggest that in vivo combination FOS + BL dosages must avoid low concentrations of FOS at the site of infection.

Anti-Bacterial Agents↗

[Value of the antibiogram for the treatment of urogenital Ureaplasma infections].

We have currently established the spectrum of sensitivity of the isolated strains to various antibiotics before prescribing the appropriate treatment. A microtechnic has been used. The most active antibiotics are: minocyclin, pristinamycin, erythromycin, chloramphenicol, oxytetracyclin, kanamycin, tobramycin, streptomycin, in this order.

Anti-Bacterial Agents↗

Phage types, antibiograms and R-plasmids of Klebsiella and Enterobacter isolated from hospital environment and food.

Four-hundred and twenty-two Klebsiella strains and 294 Enterobacter strains were isolated from direct or indirect environment of hospitalized patients, from foodstuffs, foods, culinary utensils and staff in hospital and in catering establishments. Of Klebsiella, the species K. aerogenes (76.5%) of Enterobacter, the species E. cloacae (77.6%) occurred the most frequently in all specimens. Klebsiella strains were typable in 68.5%; 53.1% of the Enterobacter strains were sensitive to phage. Most of the untypable Klebsiella and Enterobacter strains and the multiresistant strains originated from screening in hospitals. Sensitive bacteria as well as those resistant to one or two antibiotics may be potentially dangerous for the patient consuming them, since they may become multiresistant due to R-plasmid transfer.

Anti-Bacterial Agents↗

[Critical evaluation of the Abbott MS-2 automatic system in the rapid determination of antibiograms].

The Abbott MS-2 system for rapid determinations of the bacterial growths in the kinetic mode is briefly described. Some details of the operations of the apparatus and about the experimental procedure are given by referring to a phenomenological description for the bacterial growth. A critical analysis of the antimicrobic susceptibilities and of the minimum inhibiting concentrations (MIC) provided by the apparatus is also carried out. In particular the problems arising for cephalosporins is emphasized.

Ampicillin↗

[The Sceptor system: relation of results from antibiograms in diffusion agar to MIC].

The Sceptor system and the susceptibility of Gram-positive cocci and Gram-negative rods to several antibiotics were presented. The accuracy of methicillin and gentamicin MIC of Staphylococcus aureus was determined. The MIC of methicillin-resistant strain was less than 8 mg/l. Finally the MIC accuracy to 29 Pseudomonas aeruginosa strains was compared with the results obtained by the standard disk diffusion method.

Diffusion↗

Comparative evaluation of a commercial microdilution method and a conventional disk diffusion method for determination of antibiograms of gram-positive cocci.

A commercial broth microdilution system for testing the antimicrobial susceptibility of gram-positive cocci was compared with the standardized disk agar-diffusion method by testing 254 clinical strains of staphylococci and streptococci using both methods. A total of 2,794 parallel determinations were made with 92.3% complete agreement between the 2 methods; of the discrepancies encountered, 3.0% were minor, 2.5% were major, and 2.1% were very major. The results indicate that the commercial microdilution system may provide a reliable quantitative method for antimicrobial susceptibility testing of clinical isolates from animals.

Anti-Bacterial Agents↗