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Although function can be assigned to genome sequence by homology at a macroscopic level, this can be misleading in the absence of data on enzyme activities. Together, such data can reveal whether open reading frames are expressed, identify multienzyme function and point to 'orphan' function. Because of their small size and small genomes, the genome sequences of some Mycoplasma spp. are very amenable to detailed analyses.
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The burden that heart failure imposes on the health care systems of the developed world is substantial and increasing. This article reviews the world literature on the epidemiology of heart failure and discusses the key methodological issues for research in this field.
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