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PubMed1991

[Comparative study of the effect of antibiotics and antiseptics on Staphylococcus aureus].

The routinely used antibiotics and antiseptics were compared with the same staphylococcal isolates by MIC ranges, the mean MIC for the strains, proportion of the variants resistant to the drugs, distribution of the strains by the resistance spectra and the number increase rate for the resistant variants in natural populations within 5-10-year periods. It was concluded that when used locally the antiseptics had some advantages over the antibiotics especially with respect to hospital strains. The authors believe that in developing new drugs, ++re-estimation of the routinely used ones, choosing the optimal drug for a particular case it is more important to consider the heterogeneity of microbial populations, the frequency of resistant variants and activity of the mechanisms of their selection under hospital conditions.

Anti-Bacterial Agents↗
PubMed1990

[The in vitro activity of veterinary antiseptics].

The in vitro activity of 28 veterinary antiseptic drugs was studied on 4 bacterial strains either with or without calf serum. Eight preparations (VT Dose, Dulcidrine, Collyre Clément, Cronic, Detecaïne, Lotion Maudor, Tano-Patt, Aurikan), half of which were eye-lotions, were found to be non antiseptic according to AFNOR standards. In the presence of calf serum, 4 other preparations (Albacetine, Nybocaïne, Pediplasme, Pedifort) did not meet the standard NF T 72.171 criteria.

Animals↗
PubMed1990

Antiseptic toxicity to breast carcinoma in tissue culture: an adjuvant to conservation therapy?

Of 50 patients who had scrape cytology of the excision cavity after conservative surgery for breast carcinoma, 10 (20%) had malignant cells remaining in the cavity recognised by cytology. Of these patients, 18 had histological evidence of tumour at the resection margin, giving an accuracy of the cytology of 84%, a sensitivity of 56%, and a specificity of 100%. When assayed for cytotoxicity against a breast tumour cell line (MCF7) or human fibroblasts, chlorhexidine gluconate was the most effective of eight antiseptics or antitumour agents (100% cytotoxicity at a 1/10,000 dilution) in killing breast tumour cells and had 70% toxicity to human fibroblasts at the same dilution. Hydrogen peroxide appeared to be the most useful agent overall with 94% cytotoxicity to breast tumour cells with only a 12% cytotoxicity to human fibroblasts at a dilution of 1/1,000,000. We suggest that free malignant cells left in the cavity after conservative surgery for breast cancer may be a cause of local recurrence. They can be recognised by scrape cytology at operation and the topical use of antiseptics as cytotoxic agents may be beneficial and warrants further investigation.

Adult↗
PubMed1989

[Determination of bactericidal minimum concentrations of 3 antiseptics and 1 disinfectant on 580 hospital gram-negative bacilli].

Minimal bactericidal activities (MBCs) of three antiseptics (povidone iodine, chlorhexidine digluconate, benzalkonium chloride) and one disinfectant (sodium hypochloride) where determined, on 580 hospital Gram negative bacilli. Previously the Afnor T 72-150 standard for antiseptic and disinfectant was established for two reference strains E. coli CIP 54 127 and P. aeruginosa CIP A 22. No difference was found between the MBC obtained with these strains in Afnor standard and in microdilution method. Microdilution method allows to test 11 hospital isolates and one reference strain. A strain was considered as resistant when the MBC was one dilution higher than the reference strain MBC. Results were as follows: None strain was resistant to sodium hypochloride and povidone iodine; 18.2% Enterobacteriaceae were resistant to chlorhexidine digluconate with 94.2% of Proteus; 4% of Enterobacteriaceae were resistant to benzalkonium chloride with 89.5% of Proteus and only 1.8% other bacilli. Results obtained in the present study are similar as those previously published particularly with Proteus; nevertheless other studies have reported P. aeruginosa strains resistant to chlorhexidine digluconate and benzalkonium chloride; this last point was not observed in our study.

Benzalkonium Compounds↗
PubMedDate not supplied

A trial of povidone-iodine antiseptic solution for the prevention of cannula-related thrombophlebitis.

In a prospective trial of 200 adult patients, using a semiquantitative technique for culturing material from intravenous cannulae, we studied the efficacy of povidone-iodine antiseptic solution (test), and the use of no topical agent whatsoever (control). The incidence of cannula-related thrombophlebitis was slightly higher in the test group than in the control group. It is concluded that the use of povidone-iodine antiseptic solution does not reduce the incidence of cannula-related thrombophlebitis.

Adult↗
PubMed1988

[Comparative study of 3 methods of evaluation of antiseptic products and disinfectants in quality control].

For choosing a protocol of determination of bactericidal activity of antiseptic and disinfectant products, we have compared a method using an MS2 Abbott system and the classic methods: membrane filtration and dilution-method by transfer loops. The bactericidal-activity of 12 antiseptic solutions are determined. The results shown that there was no significative difference between the automated system and AFNOR specifications. The dilution-method by transfer loops gave higher bactericidal concentrations than the two other methods.

Anti-Infective Agents, Local↗
PubMed1988

The toxicology of detergents, bleaches, antiseptics and disinfectants in small animals.

Small animals are exposed to a variety of household products. The most common route of exposure is oral by direct ingestion or from grooming soiled fur and feet. The majority of household products are complex chemical mixtures. The clinician must determine the body burden of toxic or potentially toxic substances and the potential for a combination of chemicals to produce intoxication in a particular species and then plan a course of action. Toxicoses of selected household and commercial products are discussed in detail. Animals are exposed to a variety of antiseptics by numerous routes. Potential for iatrogenic intoxication from antiseptics is also discussed. An abridged version of this article will be published in the 10th edition of CURRENT VETERINARY THERAPY: SMALL ANIMAL PRACTICE, RW Kirk, editor, WB Saunders Co, Philadelphia, PA.

Animals↗
PubMed1988

[Chlorhexidine as a vaginal antiseptic in obstetrics].

The antimicrobial efficiency of a chlorhexidine containing emulsion which is used as gliding or antiseptic remedy is examined. The killer-time for germs within the suspension test as well as the testing in clinical use confirm the efficiency known from literature. The use of chlorhexidine containing emulsions serving as antiseptic gliding remedy is recommended.

Chlorhexidine↗
PubMed1986

[Proposal for a method of studying the activity of soap or antiseptic against the microbial flora of the hands after a single surgical scrub].

The authors, all members of the Antiseptics group of the Societé Française de Microbiologie, propose a standardised method for the evaluation of the activity of antiseptics on the microbial flora of the hands in the context of surgical scrubbing. This paper defines the aims of the method, its field of application and describes the media and materials required. The various phases of the preliminary study and the definitive trial also described.

Anti-Infective Agents, Local↗
PubMed1987

Comparison of topical antibiotic ointments, a wound protectant, and antiseptics for the treatment of human blister wounds contaminated with Staphylococcus aureus.

An open, randomized, human-model study was conducted to compare the effects of topical antibiotics, a wound protectant, and antiseptics on the rate of wound healing and bacterial growth using a modification of a method employing ammonium hydroxide-induced intradermal blisters inoculated with Staphylococcus aureus. Each volunteer in the study had six blister wounds (three per forearm) to which a triple antibiotic (neomycin, polymyxin B, bacitracin) ointment or one of four other test agents was applied twice a day. A control wound remained untreated. All wounds were covered with an occlusive dressing after treatment. The time to healing (100 percent epithelialization) was evaluated for each wound. Wounds were cultured for bacterial growth after two treatments. Contaminated blister wounds treated with the triple antibiotic ointment healed significantly faster (mean nine days) than wounds treated with any antiseptic and those receiving no treatment. Only the neomycin-polymyxin B-bacitracin combination effectively eliminated bacterial contamination of the wounds after two applications (within 16 to 24 hours after contamination with Staphylococcus aureus). The overall clinical appearance and healing rates of wounds treated with the triple antibiotic were ranked superior to all treatments (and no treatment) except the other antibiotic ointment in the study.

Administration, Cutaneous↗
PubMed1977

[Bactericidal power's assessment of eight antiseptic products intended to surgeon's hand-washing (author's transl)].

A standard hand-washing technique was used in order to test the relative effectiveness of eight both detergent and alcoholic preparations intended to surgeon's hands disinfection. A single four or seven minutes washing with alcoholic solutions was shown to eliminate a much larger proportion of the skin flora than could be removed by a single four or seven minutes hand-washing with detergent antiseptic preparations. Authors have also determined effect of wearing surgical rubber gloves after skin disinfection and compared viable bacterial counts in hand washings immediately after the antiseptic treatment and when gloves had been worn for one hour.

Bacteriological Techniques↗
PubMed1986

Comparison of antipseudomonad activity of chlorine dioxide/chlorous acid-containing gel with commercially available antiseptics.

A chlorine dioxide-containing gel was compared with 3 commercially available antimicrobials and 1 antibiotic intended for topical use. This gel was tested at 0.5 X and 4 X and was found to be more effective as a 4 X gel in treating Pseudomonas aeruginosa-infected excised wounds on mice. To further compare this gel with other antiseptics, a murine bioassay was developed. This wound model consisted of an excised cutaneous wound on the dorsum of mice which were irradiated (800 rad) and inoculated with P aeruginosa at 10-fold dilutions, from 10(-2) to 10(-10). The wounds were observed for latency of infection or mice survival time as a function of concentration of viable organisms remaining after treatment. The advantage of this model was demonstrated where a standard curve based on latency did not consume as many test subjects and yet provided an estimate of viable organisms in each wound. In this model, the chlorine dioxide-containing gel was more active than were preparations of providone-iodine, chlorhexidene, or silver sulfadiazine and was similar to polymyxin-bacitracin-neomycin ointment as a topical antiseptic. The effectiveness of the tested gel was reduced if delays in treatment were longer than 1 hour.

Animals↗
PubMed1985

[In vitro studies on Geliperm Dry as a vehicle for antibiotics and antiseptics in local burn therapy].

Geliperm is a synthetic skin substitute, consisting of polysaccharid-polyacrylic plates. It is available as a soaked or a dried form (Geliperm dry). Geliperm dry can be soaked in antibiotic or antiseptic solutions. The in vitro inhibition of bacterial growth has been measured. The optimal concentrations have been evaluated. Geliperm dry soaked in antibiotic or antiseptic solutions can be used in the treatment of burns, but does not replace early excision of burned tissue or infected necrosis.

Acrylamides↗
PubMed1977

Tissue toxicity of antiseptic solutions. A study of rabbit articular and periarticular tissues.

The gross, histologic, and biochemical effects of four commercially available antiseptic solutions on rabbit knee articular and periarticular connective tissues were investigated. Rabbit knee joints were injected with 2.0 cc of either Betadine prep solution, Betadine scrub solution, pHisohex or 3% hexachlorophene. The opposite knees were injected with sterile saline. All animals were injected three times at 48-hour intervals and sacrificed 10 days after the last injection. The solutions containing detergents, Betadine scrub, and pHisohex caused severe gross and histologic damage to articular cartilage, synovia, and muscle. The hexachlorophene loss solution caused moderate histologic damage, but caused articular cartilage ground substance. Betadine prep solution caused only minimal gross and histologic damage, without any biochemical evidence of articular cartilage damage. If antiseptic solutions are to be used in irrigating or packing joint injuries, the use of Betadine prep is recommended.

Animals↗
PubMed1984

[Evaluation of the cytotoxicity of an antiseptic by a photometric micromethod].

Cytotoxicity of an antiseptic is usually evaluated by microscopic examination of a cell culture, after a set time of contact with the antiseptic. As this evaluation is largely subjective, a photometric method is proposed. The procedure consists in staining the cells by methylene blue after contact with the ATS and measuring the dye in a photometer after elution. Different dilutions of ATS are added to a 24 h-monolayer of Vero cells in a 96-well microtissue culture plate (8 wells for each dilution, 8 wells for cell control, and 8 wells for control of dye fixation by the plastic plate). The plate is incubated for three days at 37 degrees C in an atmosphere of 5% CO2 and 95% air. The plate is washed with PBS to remove dead cells and adherent cells are fixed. The plate is then washed with borate buffer and allowed to dry. The dye is eluted by adding 200 microliter of HCl 0.1 N to each well. The plate is then read automatically at 650 nm on a Titertek Multiskan, a vertical light path photometer. Cytotoxicity is expressed as the percentage of damaged cells as compared to control wells. Cytotoxic assay of glutaraldehyde shows that this technique is more reliable and more sensitive than microscopic examination. Moreover, result of cytotoxic assay and of a method consisting in measurement of protein in residual cells after exposure to ATS are significantly correlated.

Animals↗
PubMed1984

[Effectiveness of 9 soaps and/or antiseptics on hand flora after surgical-type washing].

We studied the effect of nine soaps and/or antiseptics on the bacterial flora of hands 5 minutes after a surgical scrub. Each agent was used by ten healthy volunteers, free of skin lesions. The following agents were used: chlorhexidine gluconate 4% and 1.5%, povidone iodine 4%, ethanol 70 degrees, isopropanol 70 degrees, a non-antiseptic soap, and another soap followed by either ethanol 70 degrees, isopropanol 70 degrees or a preparation containing H2O2. The surgical scrub procedure varied slightly according to whether or not the agent was soapy and required rinsing. Sampling was carried out using Gaschen's bag method with 400 ml of neutralising solution. Counts were made after 48 hours aerobic incubation at 35 degrees C on tryptic soy agar with 1% Tween 80, and after 8 days anaerobic incubation at 35 degrees C on Brewer's yeast agar with 1% Tween 80. Results were expressed as the log 10 to the number of bacteria per hand. Statistical significance was determined using the Student t test. The greatest reduction in aerobic flora was produced by isopropanol 70 degrees C (1.7 log 10). 1.5 to 0.5 log 10 reductions were produced, in the following decreasing order, by ethanol 70 degrees, povidone iodine, chlorhexidine gluconate 4% and 1.5% and a soap with ethanol 70 degrees. A reduction of less than 0.5 log 10 was produced by a soap with isopropanol 70 degrees, and soaps with H2O2. Similar results were obtained with the facultative anaerobes.

Anti-Infective Agents, Local↗
PubMed1984

[Experimental models for the study of antiseptics].

Determination of the bactericidal concentration of antiseptics in vitro is now standardized, allowing interesting comparisons. Conversely, measurement of this activity in vivo in man is difficult since results are dependent upon skin sampling techniques and variations in bacterial growth from individual to individual and from one site to the next. Skin sampling can be standardized by using the hologenic Hairless mouse as an experimental model (skin needle biopsy and crushing). However, variations from one animal to the next remain a problem, even though results are very similar to those found in man. We therefore used monoxenic Hairless mice contaminated with S. aureus, S. epidermidis and P. aeruginosa. All experiments on these animals demonstrated consistency of bacterial cutaneous and digestive growth over time and smallness of variations in bacterial flora from animal to animal. We advocate use of this second model for standardization of evaluation of bactericidal activity of antiseptics.

Animals↗