[Effects of prostaglandins (PGE1) on the development of amebas i experimentally infected rats].
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When comparing the results of coprological tests carried out in optimal conditions among 200 male homosexuals (MHS) (1) and 500 male heterosexuals, most of whom were suffering from intestinal disorders, it was found that 52% of MHS and only 3.6% of heterosexuals were Ent. histolytica carriers. The 14 to 1 ratio shows the "uncorrected" relative risk of amoebiasis among MHS, which becomes a 10-12 to 1 corrected relative risk, after taking into account intervening factors. Travelling to the tropics does not increase the incidence of amoebiasis among examined MHS. The occurrence rates of Ent. coli, End. nana, Giardia are higher among MHS, and the rates of two species which are usually rare, Pseudolimax (or Iodamoeba) and Enteromonas (flagellate of the colon), rise sharply in this group, although the occurrence rates of the other protozoa stay at the same level in both groups. Presently, we are unable to explain fully these different behaviors. The highly increased presence of intestinal protozoa and the unusual prevalence of some species among the MHS condition numerous and diversified parasitic associations. Some associations found only among MHS indicate oro-faecal contamination which is the only way for direct transmission of protozoa between individuals.
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Thermotolerance and resistance to ethyl alcohol were investigated in Amoeba proteus of polyploid series. Thermoresistance of these amoebae was determined by measuring the mean time of survival at the lethal temperature (43 degrees C). The resistance to ethyl alcohol was estimated by the mean percentage of individuals that survived following a 5-minute treatment with 7.5% ethyl alcohol. Neither thermotolerance, nor ethyl alcohol resistance were found to depend on the amount of nuclear DNA. It was also established that colchicine did not induce a hereditary instability of thermoresistance in amoebae.
Fourteen strains of amoebae of the Limax group were isolated by random selection in five species of farm and domestic animals. The amoebae were captured in the respiratory and digestive tracts and in sex organs. According to the systematic classification, the isolates belong to the following genera: Acanthamoeba, Hartmannella, and Vahlkampfia. Their temperature optimum was 37 degrees C. The trial to demonstrate a pathogenetic character of the representative of individual genera on laboratory animals gave a negative result.
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