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At least 289 records · Page 16Linked to original sources
PubMed2005

Central serotonin 3 receptors play an important role in the modulation of nociceptive neural activity of trigeminal subnucleus caudalis and nocifensive orofacial behavior in rats with persistent temporomandibular joint inflammation.

The role of central serotonin 3 receptors on neural activities recorded from superficial laminae of trigeminal subnucleus caudalis/upper cervical spinal cord junction region was investigated using rats with (Complete Freund's Adjuvant day 7 group) or without (non-Complete Freund's Adjuvant group) persistent temporomandibular joint inflammation evoked by Complete Freund's Adjuvant for 7 days. We identified two types of units, Deep-wide dynamic range units and Skin-wide dynamic range units from extracellular recordings. Deep-wide dynamic range units have mechanoreceptive fields in the deep craniofacial tissues including masseter muscle but do not have cutaneous mechanoreceptive fields. Deep-wide dynamic range unit discharges evoked by the formalin injection into masseter muscle were significantly enhanced in the late phase in Complete Freund's Adjuvant day 7 group. Discharges of Skin-wide dynamic range units evoked by the noxious pinch stimulation to facial skin in Complete Freund's Adjuvant day 7 group were significantly enhanced compared with those in non-Complete Freund's Adjuvant group. Topical administration of central serotonin 3 receptor antagonist, tropisetron, onto trigeminal subnucleus caudalis/upper cervical spinal cord junction region significantly reduced both formalin-evoked Deep-wide dynamic range unit and pinch-evoked Skin-wide dynamic range unit discharges in non-Complete Freund's Adjuvant and Complete Freund's Adjuvant day 7 groups significantly. The inhibitory effects of tropisetron on pinch-evoked Skin-wide dynamic range unit discharges were prolonged in Complete Freund's Adjuvant day 7 group compared with those in non-Complete Freund's Adjuvant group. The role of central serotonin 3 receptors in trigeminal subnucleus caudalis/upper cervical spinal cord junction region was also tested by orofacial formalin test in Complete Freund's Adjuvant day 7 group. Intracisternal administration of tropisetron decreased the orofacial nocifensive behavior in the late phase evoked by the injection of formalin into the masseter muscle. These results suggest that central serotonin 3 receptors in trigeminal subnucleus caudalis/upper cervical spinal cord junction region are involved in mediating pronociceptive effects in both superficial and deep craniofacial tissues nociception during persistent temporomandibular joint inflammation.

Action Potentials↗
PubMed2006

Linear and nonlinear hearing aid fittings--2. Patterns of candidature.

We studied candidature for linear, slow-acting AVC hearing aids, and fast-acting WDRC hearing aids in a within-subject within-device crossover design of 50 listeners with SNHL. Candidature dimensions include HTLs, ULLs, spectro-temporal and masking abnormalities, cognitive capacity, and self-reports and acoustic measures of auditory ecology. Better performance with linear fittings is associated with flatter audiograms, wider dynamic range, and smaller differences in dynamic range between low and high frequencies, and also with more restricted auditory lifestyles. Better performance with all nonlinear fittings is associated with more sloping audiograms, more restricted dynamic ranges, greater differences in dynamic range between low and high frequencies, and more varied auditory lifestyles. Differential performance between WDRC and AVC fittings is associated with patterns of variation in auditory ecology (rapid versus slow changes) and cognitive (high versus low) capacity. Differential performance between WDRC in two channels, and a hybrid with WDRC in a low-frequency and AVC in a high-frequency channel is associated with psychoacoustic tests of cochlear function (high susceptibility to spectral and temporal smearing, and high susceptibility to upward spread of masking respectively). Patterns of candidature include measures beyond auditory function in the domains of cognitive capacity and auditory ecology.

Aged↗
PubMed1994

Effect of compression ratio in a slow-acting compression hearing aid: paired-comparison judgments of quality.

Paired-comparison judgments of quality were obtained from 20 hearing-impaired listeners (half with a small dynamic range and half with a large dynamic range) for speech-in-noise (vent, apartment, and cafeteria) processed through a slow-acting compression hearing aid. Compression ratio was varied (1, 1.5, 2, 3, 5, and 10:1). Compression threshold, attack time, and release time were fixed. Sound quality judgments were significantly affected by compression ratio, noise, and dynamic range. Preference decreased with increasing compression ratio. The selection of compression ratio. The selection of compression ratios < or = 2:1 was significantly higher than of compression ratios > 3:1. Less compression (no compression or 1.5:1) was preferred with the highest level noise (cafeteria noise) than with the lower level noises (vent or apartment). In particular, the small dynamic range group preferred compression with the vent and apartment noises (noise below the compression threshold), but preferred a linear hearing aid with the cafeteria noise (above the compression threshold). The large dynamic range group showed a slightly greater preference for the linear hearing aid for all three noises.

Adolescent↗
PubMed2000

Quantitative reverse transcription-polymerase chain reaction to study mRNA decay: comparison of endpoint and real-time methods.

Four quantitative reverse transcription-PCR (RT-PCR) methods were compared to evaluate the time course of mRNA formation and decay. Mouse fibroblasts (NIH 3T3) transfected with the human beta-globin open reading frame/c-myc 3'-untranslated region chimeric gene under control of the c-fos promoter (fos-glo-myc) were used for serum-inducible transcription. The amount of fos-glo-myc mRNA, relative to beta-actin, was measured by quantitative, RT-PCR at various times following the addition of serum to serum-starved fibroblasts transfected with the chimeric gene. Both endpoint (band densitometry and probe hybridization) and real-time (SYBR green and TaqMan) PCR methods were used to assay the identical cDNA. The real-time methods produced a 4- to 5-log dynamic range of amplification, while the dynamic range of the endpoint assays was 1-log. The real-time and probe hybridization assays produced a comparable level of sensitivity that was considerably greater than band densitometry. The coefficient of variation from 22 replicate PCR reactions was 14.2 and 24.0% for the SYBR green and TaqMan detection, respectively, and 44.9 and 45.1% for the band densitometry and probe hybridization assays, respectively. The rank order for the values of r(2) obtained from the linear regression of the first-order mRNA decay plots was SYBR green > TaqMan > probe hybridization > band densitometry. Real-time PCR is more precise and displays a greater dynamic range than endpoint PCR. Among the real-time methods, SYBR green and TaqMan assays produced comparable dynamic range and sensitivity while SYBR green detection was more precise and produced a more linear decay plot than TaqMan detection.

3T3 Cells↗
PubMed1994

Suspension microlaryngoscopic surgery and indirect microlaryngostroboscopic surgery for benign lesions of the vocal folds.

A prospective study was designed to compare the effects on voice capacities after either suspension microlaryngoscopic surgery or indirect microlaryngostroboscopic surgery. Patients where the clinical diagnosis 'dysphonia due to a benign lesion of the vocal fold' was made, and who could be operated in either way, entered the study. Post-operative voice evaluation was performed on 21 patients after suspension microlaryngoscopic or indirect microlaryngostroboscopic surgery. The long-term voice results following indirect microlaryngostroboscopic surgery and suspension microlaryngoscopic surgery demonstrate a statistically significant improvement for the maximum intensity, maximum dynamic intensity range, dynamic intensity range at habitual speaking pitch, and melodic pitch range. In selected cases indirect microlaryngostroboscopic surgery offers a very good functional result.

Adolescent↗
PubMed1979

[Loss of hearing in cerebral palsy (author's transl)].

The hearing threshold level was examined in 75 cerebral palsied children (3--15 yrs.). In the case of 5 patients, we were unable to get an audiogram. 50 children had a normal audiogram on both ears. 13 patients had a bilateral, one a unilateral sensoneural loss of hearing. The rate of conductive loss of hearing was unexpectedly high: 4 patients with a bilateral pure conductive loss of hearing, 3 with a bilateral sensoneural loss of hearing and a bilateral additional airborne gap, and 2 with a monaural conductive loss of hearing. The treatment of the conductive deficiency is of primary importance in cases of a super-imposed senso-neural loss of hearing losses. 7 of our patients have been fitted with a hearing aid. The threshold of the stapedius reflex could be estimated in the case of 10 patients suffering from a sensoneural loss of hearing. All had a reduced dynamic range. Reduction of the dynamic range is not a specific trait of cerebral palsy as our cerebral palsied children with normal hearing had a normal dynamic range. No correlation could be found between asphyxia, underweight and hyperbilirubinaemia as single factors in the newborn period and sensoneural loss of hearing nor between athetosis and sensoneural loss of hearing.

Adolescent↗
PubMed1989

Coding of free field intensity in the auditory midbrain of the leopard frog. I. Results for tonal stimuli.

Single unit recordings in the auditory midbrain of the leopard frog were obtained in response to free field, random frequency, tone pip stimulation. Five sequences of 81 logarithmically distributed frequencies from 100 Hz to 3200 Hz inclusive at stimulus levels between 94 dB SPL and the unit's threshold were presented. Sigmoidal response-intensity functions were fit through the data points for monotonic and nearly monotonic units (53 in total) at all frequencies for which the units were responsive. From the fitcurves a generalized slope and inflection point were obtained and converted into a dynamic range and a threshold value. Single unit dynamic ranges at CF frequencies were between 5 and 80 dB, and thresholds were between 20 dB and 90 dB. For the population of units the dynamic ranges were positively correlated with the threshold values. A population response was computed by adding the response at each intensity-frequency combination, and again curve fitting with the sigmoidal function. The population dynamic range was about 70 dB for the basilar papilla range (around 1200 Hz) and somewhat less, about 45 dB, for the amphibian papilla neurons. The amount of synchronization of the firings of the individual units with the stimulus was calculated on basis of the shifted auto-coincidence function. The stimulus synchronization appeared to be largely independent of stimulus intensity. Inter-neuron synchronization was computed on basis of the cross-coincidence function of two neuronal spike trains. Also this synchronization was stimulus independent. It was concluded that synchronization or other forms of multiplicative action cannot on its own code for stimulus intensity. From the application of signal detection theory it is concluded that a rate coding on basis of a modest population of units has an acceptable dynamic range, roughly covering 30-100 dB SPL, produces a more than adequate sensitivity for intensity differences and may be used as a code for intensity.

Acoustic Stimulation↗
PubMed2000

Extended-range glucose sensor employing engineered glucose dehydrogenases.

An enzyme glucose sensor with an expanded dynamic range was constructed using a novel strategy. This strategy was based on a new concept of utilizing protein-engineered enzymes with a different Michaelis constant, which allows for the expanded dynamic range. We used the engineered Escherichia coli pyrroloquinoline quinone glucose dehydrogenase (PQQGDH) of which His775 was substituted for Asp which showed an increased Km value (25-fold). We first constructed the composite colorimetric analytical system employing the wild-type PQQGDH and His775Asp and evaluated its dynamic range. The composite colorimetric analytical system was constructed and showed a wide dynamic range of 0.5-30 mM with less than +/-5% error. The composite colorimetric analytical system, an extended-range colorimetric analytical system, enabled the determination of the concentration of glucose over a 30-fold range that could not have been achieved using the single colorimetric analytical system. Furthermore, we have demonstrated the composite amperometric glucose sensor employing the combination of His775Asn and His775Asp. The extended-range glucose sensor acquired not only the expanded dynamic range (3-70 mM) that covered both dynamic ranges of the single enzyme sensors but also the narrower substrate specificity of glucose due to the inherited property of engineered enzymes.

Biosensing Techniques↗
PubMed1995

Preferred listening levels for linear and slow-acting compression hearing aids.

OBJECTIVE: The purpose of the present experiment was to determine the relationship between most comfortable listening level and preferred listening levels for linear and slow-acting compression hearing aids as a function of variations in speech and noise level. DESIGN: A digital hearing aid test system was used to simulate six hearing aids having compression ratios of 1, 1.5, 2, 3, 5, and 10:1. Speech was presented in three different noises (vent, apartment, and cafeteria), with speech input level being varied (55, 70, 85 dB SPL). Subjects were 20 listeners with sensorineural hearing loss (half with a dynamic range < or = 30 dB and half with a dynamic range >30 dB). The boundaries of the most comfortable listening range were measured to estimate most comfortable listening level. Preferred listening level was measured by having subjects adjust the output of the hearing aid for satisfactory listening. RESULTS: On average, the deviation of preferred listening level from most comfortable loudness (MCL) was less than 5 dB. Dynamic range, noise type, and input level were all found to have small, but significant, effects on the deviation of preferred listening level from MCL. On average, subjects with a small dynamic range listened slightly below MCL, and subjects with a larger dynamic range listened slightly above MCL. For favorable signal-to-noise ratios, preferred listening levels were highest for high input levels and for conditions that resulted in high output levels before level adjustment. Although the pattern of average performance differed slightly at poorer signal-to-noise ratios, all preferred listening levels were close to MCL. CONCLUSIONS: The gain of a slow-acting compression hearing aid should place the output within 5 dB of MCL. The output for low and medium inputs should approximate MCL and the output for high input levels should be slightly above MCL. This pattern of gain may be obtained with mild compression ratios and a gain rule that places a speech input of 70 dB at MCL.

Audiometry, Pure-Tone↗
PubMed1996

Intensity discrimination as a function of stimulus level with electric stimulation.

Difference limens (DLs) for changes in electric current were measured from multiple electrodes in each of eight cochlear-implanted subjects. Stimuli were 200-microseconds/phase biphasic pulse trains delivered at 125 Hz in 300-ms bursts. DLs were measured with an adaptive three-alternative forced-choice procedure. Fixed-level psychometric functions were also obtained in four subjects to validate the adaptive DLs. Relative intensity DLs, specified as Weber fractions in decibels [10 log (delta I/I)] for standards above absolute threshold, decreased as a power function of stimulus intensity relative to absolute threshold [delta I/I = beta (I/I0) alpha] in the same manner as Weber fractions for normal acoustic stimulation reported in previous studies. Exponents (alpha) of the power function for electric stimulation ranged from -0.4 to -3.2, on average, an order of magnitude larger than exponents for acoustic stimulation, which range from -0.07 to -0.11. Normalization of stimulus intensity to the dynamic range of hearing resulted in Weber functions with similar negative slopes for electric and acoustic stimulation, corresponding to an 8-dB average improvement in Weber fractions across the dynamic range. Sensitivity to intensity change ¿10 log beta¿ varied from -0.42 to -13.5 dB compared to +0.60 to -3.34 dB for acoustic stimulation, but on average was better with electric stimulation than with acoustic stimulation. Psychometric functions for intensity discrimination yielded Weber fractions consistent with adaptive procedures and d' was a linear function of delta I. Variability among repeated Weber-fraction estimates was constant across dynamic range. Relatively constant Weber fractions across all or part of the dynamic range, observed in some subjects, were traced to the intensity resolution limits of individual implanted receiver/stimulators. DLs could not be accurately described by constant amplitude changes, expressed as a percentage of dynamic range ¿delta A(% DR)¿. Weber fractions from prelingually deafened subjects were no better or worse than those from postlingually deafened subjects. The cumulative number of discriminable intensity steps across the dynamic range of electric hearing ranged from as few as 6.6 to as many as 45.2. Physiologic factors that may determine important features of electric intensity discrimination are discussed in the context of a simple, qualitative, rate-based model. These factors include the lack of compressive cochlear preprocessing, the relative steepness of neural rate-intensity functions, and individual differences in patterns of neural survival.

Auditory Threshold↗
PubMed1988

Single-tone intensity discrimination based on auditory-nerve rate responses in backgrounds of quiet, noise, and with stimulation of the crossed olivocochlear bundle.

We use simple statistical models of the firing patterns of high, medium, and low spontaneous rate auditory-nerve fibers to study mechanisms which determine the overall dynamic range of the auditory periphery. The models relate experimentally measured rate response properties of fibers with best frequency (BF) near 8.0 kHz to their ability to encode changes in BF tone level by changes in discharge rate in backgrounds of quiet and noise, with and without electrical stimulation of the crossed olivocochlear bundle (COCB). Application of the models to the BF tone rate responses of auditory-nerve fibers in backgrounds of quiet shows that optimum processing of the rate responses of fibers with BF near 8.0 kHz yields performance in the intensity discrimination task meeting or exceeding that of human subjects over an 80 dB range of levels. By defining a statistical measure of dynamic range, we confirm the results of Costalupes et al. (1984) demonstrating that masking noise shifts the dynamic range of auditory-nerve fibers to higher stimulus levels, thus preventing rate saturation. However, model analysis shows that masking noise also produces large reductions of dynamic range as well as large increases in the minimum intensity difference that can be encoded by the rate responses of single and ensembles of fibers. Electrical stimulation of the COCB can restore auditory-nerve fiber dynamic range and sensitivity to changes in BF tone level in noise backgrounds, in some cases to roughly that observed in backgrounds of quiet.

Animals↗
PubMed2006

Presynaptic inhibition modulates spillover, creating distinct dynamic response ranges of sensory output.

Sensory information is thought to be modulated by presynaptic inhibition. Although this form of inhibition is a well-studied phenomenon, it is still unclear what role it plays in shaping sensory signals in intact circuits. By visually stimulating the retinas of transgenic mice lacking GABAc receptor-mediated presynaptic inhibition, we found that this inhibition regulated the dynamic range of ganglion cell (GC) output to the brain. Presynaptic inhibition acted differentially upon two major retinal pathways; its elimination affected GC responses to increments, but not decrements, in light intensity across the visual scene. The GC dynamic response ranges were different because presynaptic inhibition limited glutamate release from ON, but not OFF, bipolar cells, which modulate the extent of glutamate spillover and activation of perisynaptic NMDA receptors at ON GCs. Our results establish a role for presynaptic inhibitory control of spillover in determining sensory output in the CNS.

Action Potentials↗
PubMed1997

A digital filterbank hearing aid: three digital signal processing algorithms--user preference and performance.

OBJECTIVE: Three digital signal processing algorithms named RangeEar, DynEar, and LinEar were compared with regard to user preference and performance when a wearable digital filterbank hearing aid was used. All three algorithms provided individual frequency shaping via a seven-band filterbank. Compression was used in a low-frequency (LF) and a high-frequency (HF) channel. RangeEar and DynEar used wide dynamic range syllabic compression in the LF channel, whereas LinEar used compression limiting. In the HF channel, RangeEar used a slow acting automatic volume control, whereas DynEar and LinEar used compression limiting. The subjects had access to a manual volume control when using the LinEar or DynEar options. DESIGN: The study included 13 hearing aid users with symmetrical sensorineural losses. In a 1 mo long blind field test, the RangeEar algorithm was compared with the preferred algorithm from an earlier study, DynEar or LinEar. A data logger function was included for objective recording of the total time each algorithm was used and how the volume controls were used. The preference was based on the time used for each algorithm and from subjective statements. Threshold signal-to-noise ratio (S/N-threshold) for speech was tested, and sound quality ratings were obtained through a questionnaire. RESULTS: Of the 13 subjects, six preferred the RangeEar fitting and another four preferred the DynEar fitting. Two subjects preferred the LinEar fitting and one had equal preference for RangeEar and LinEar. The results from the questionnaire showed that the preferred fittings were rated higher concerning overall impression of sound quality and clearness, whereas the S/N for the speech test did not show any differences. Preferences, where stated, could be predicted from auditory dynamic range measurements in the LF and HF frequency ranges. The mean dynamic range was broader for low and narrower for high frequencies for those who preferred the RangeEar or DynEar fitting as compared with those who preferred the LinEar fitting. The preference between RangeEar and DynEar was predicted by differences in the HF range, with the narrower dynamic range for the DynEar preference subjects. CONCLUSION: Most subjects preferred the option of having a wide dynamic range syllabic compressor in the LF channel and having the overall gain in the HF channel adjustable, either manually (DynEar) or automatically (RangeEar).

Adult↗
PubMed1978

Accommodation dynamics I. Range nonlinearity.

We show the presence of a new nonlinearity in accommodation dynamics. Positive accommodation is slower when the eye is operating in the near range; conversely, relaxation of accommodation is apparently slower during work in the far range. Latency of accommodation is unaffected by working range. This new dynamic range nonlinearity has been defined in connection with a continuing research study of accommodation responses in normal subjects and in clinic patients. Instrumentation and analysis procedures are described.

Accommodation, Ocular↗
PubMed1989

Optical plankton analyser: a flow cytometer for plankton analysis, II: Specifications.

An analysing flow cytometer, the optical plankton analyser (OPA), is presented. The instrument is designed for phytoplankton analysis, having a sensitivity comparable with commercially available flow cytometers, but a significantly extended particle size range. Particles of 500 microns in width and over 1,000 microns in length can be analysed. Sample flow rates of up to 55 microliters/s can be used. Also, the dynamic range of the instrument is significantly increased for particles larger than about 5 microns. The optics, hydraulics, and electronics of the instrument are described, including the best form for a low fluid shear cuvette. The new pulse quantification technique we call digital integration is presented. This technique is essential for the instrument to handle both short and very long particles with a large dynamic range. Test measurements demonstrating particle size range and dynamic range are presented. Dynamic ranges of 10,000 and 100,000 were typically observed, measuring field samples with Microcystis aeruginosa colonies, whereas one sample showed a dynamic range of 10(6). A simple method for interpretation of time of flight (TOF) data in terms of particle morphology is presented. The specifications of the instrument are given.

Electronic Data Processing↗
PubMed1989

Coding of tone-pulse amplitude by single neurons in auditory cortex of albino rats (Rattus norvegicus).

We examined the neural representation of tone pulse amplitude in the auditory cortex of anesthetized albino rats. Rate-level functions for monaural, contralateral CF tones were obtained from single neurons. Most of these functions were saturating and monotonic in shape. The dynamic ranges of these functions were typically 5 to 35 dB in breadth, although the tail of this distribution extended to beyond 60 dB. The neurons with the widest dynamic ranges were usually those with the lowest CF thresholds. Nonmonotonic neurons were uncommon, and the nonmonotonicity was not as well developed as that seen in the cortex of cats and monkeys. For two individual rats, data are presented for neurons tuned to the same tone frequency in a single cerebral hemisphere. The CF thresholds of these neurons varied over a 50 dB range, and their collective dynamic ranges spanned at least 70 dB. These data provide a minimum estimate of the dynamic range of the cortical code for tone pulse amplitude in the rat. They suggest that there may be no serious mismatch between the neural and behavioral amplitude dynamic ranges.

Acoustic Stimulation↗
PubMed1987

Effect of electrical stimulation of the crossed olivocochlear bundle on auditory nerve response to tones in noise.

The discharge rates of single auditory-nerve fibers responding to best-frequency (BF) tones of varying level presented simultaneously with fixed level broadband noise were recorded with and without electrical stimulation of the crossed olivocochlear bundle (COCB). In the absence of COCB stimulation, monotonic increases in noise level produce monotonic increases in the low-level noise-driven response rate of auditory nerve fibers. As a result of adaptation, these increases in noise-driven response rate produce monotonic decreases in saturation discharge rate. At high noise levels, these compressive effects may eliminate the differential rate response of auditory nerve fibers to BF tones. COCB stimulation can restore this differential rate response by producing large decreases in noise-driven response rate and large increases in saturation discharge rate. In backgrounds of quiet, COCB stimulation is known to shift the dynamic range of single auditory nerve fiber BF tone responses to higher stimulus levels. In the presence of background noise, COCB stimulation produces upward shift of dynamic range, which decreases with increasing noise level. At high noise levels, COCB-induced decompression of rate-level functions may occur with little or no dynamic range shift. This enables auditory nerve fibers to signal changes in tone level with changes in discharge rate at lower signal-to-noise ratios than would be possible otherwise. Broadband noise also produces upward shift of the dynamic range of single auditory nerve fiber BF tone response. Noise-induced dynamic range shift of BF tone response was measured as a function of noise level with and without COCB stimulation. COCB stimulation elevates the threshold of noise-induced dynamic range shift. This shift is thought to result from two-tone rate suppression. Increases in the threshold of noise-induced shift due to COCB stimulation therefore suggests an interaction between the mechanism of two-tone rate suppression and the mechanism by which COCB stimulation produces dynamic range shift. These interactions were further investigated by recording auditory nerve fiber rate responses to fixed-level BF excitor tones presented simultaneously with fixed-frequency variable level suppressor tones. Rate responses were recorded with and without COCB stimulation. Experimental results were quantified using a phenomenological model of two-tone rate suppression presented by Sachs and Abbas.

Acoustic Stimulation↗
PubMed1991

Direct electrical stimulation of the cochlear nucleus: surface vs. penetrating stimulation.

Prosthetic stimulation of the cochlear nucleus (CN) has been used for rehabilitation of profoundly deaf patients who are not suitable candidates for cochlear implants. The goal of this article was to assess the relative effectiveness of surface vs. penetrating stimulation of the CN. Electrophysiologic and autoradiographic measures were used to study central auditory system activation elicited by direct stimulation of the CN. Eighteen pigmented guinea pigs, divided into three groups, underwent acute implantation of bipolar electrodes in the CN. One group was not stimulated and acted as a control (n = 7). Electrodes were placed on the surface of the CN in one test group (n = 4) and within the CN in a second test group (n = 7). Thresholds for electrically evoked middle latency responses (EMLR) were determined and input/output (I/O) functions were obtained. The two test groups were then pulsed with [14C]-2-Deoxyglucose (2-DG) intramuscularly and stimulated for 1 hour with biphasic; charge-balanced pulses having a total duration of 400 microseconds, a repetition rate of 100/sec, and an amplitude of 200 microA. After stimulation, animals were killed and brains were harvested and prepared for autoradiography using standard techniques. Threshold current for EMLRs in the surface-stimulated group had a mean of 67.5 +/- 23.9 microA (range, 40 to 100 microA). Thresholds for in-depth stimulated group had a mean of 11.4 +/- 3.5 microA (range, 10 to 20 microA). The saturation level of the I/O function for the surface-stimulated group had a mean of 287.5 +/- 41.5 microA (range, 250 to 350 microA). The saturation level for the in-depth stimulated group had a mean of 192.9 +/- 49.5 mciroA (range, 100 to 250 microA). The dynamic range for the surface electrodes had a mean of 13.1 +/- 2.7 dB (range, 9.9 to 15.9 dB), whereas the dynamic range for the penetrating electrodes had a mean of 24.5 +/- 2.6 dB (range, 20 to 28.0 dB). Autoradiographs generated by CNS tissue from stimulated animals demonstrated no significant difference in metabolic activity of the CN between surface and in-depth stimulated groups. However, there were highly significant differences in 2-DG uptake in the contralateral superior olivary complex, contralateral inferior colliculus, and ipsilateral and contralateral lateral lemniscus, with greater uptake in in-depth stimulated preparations. Electrophysiologic and autoradiographic data suggest that a penetrating CN prosthesis is capable of activating the auditory tract at a lower threshold, with a relatively wider dynamic range than a surface prosthesis.(ABSTRACT TRUNCATED AT 400 WORDS)

Animals↗