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Field trials of a new sub-acute rodenticide flupropadine, against wild Norway rats (Rattus norvegicus).

Fifteen experimental treatments with rodenticidal baits containing 0.1, 0.15 or 0.2% flupropadine were conducted on farmsteads against Rattus norvegicus infestations. Eight treatments were completely successful and the others gave kills ranging from 36 to 72% in 28 days. Treatments with 0.1 and 0.15% flupropadine were less successful against large infestations than against small ones. Flupropadine was most successful at 0.2% but still gave incomplete kills on farms where abundant alternative food was available. The compound was more effective than acute poisons in achieving complete control of Norway rat infestations, but was less reliable in doing so than anticoagulants. On the other hand, many flupropadine treatments gave quicker control and used smaller quantities of bait than anticoagulant treatments.

Animals↗

Field trials of the rodenticide gophacide against wild house mice (Mus musculus L.).

The acute rodenticide gophacide was tested against urban infestations of the house mouse (Mus musculus L.) and treatment success was assessed from the results of census baitings conducted before and after each treatment. Seven of eight populations of mice living in premises where alternative food supplies were limited were successfully controlled when medium oatmeal bait containing gophacide at 0.1% was laid directly for 4 days. In further treatments against mice inhabiting more complex environments and having greater access to other foods, the performance of gophacide at 0.1% and at 0.25% in a wholemeal flour/pinhead oatmeal/corn oil bait was compared with that of zinc phosphide at 3.0% in the same bait-base. The poison treatments were conducted for 1 or 4 days and always after 3 days pre-baiting. Treatment success varied considerably irrespective of the type of treatment or of the poison used. In general, however, gophacide proved to be as effective as zinc phosphide for the control of mice.

Amidines↗

A standard procedure for measuring rodent bedding particle size and dust content.

Hardwood dust can cause dermatitis, respiratory disease, allergies and nasal cancer in humans. A major concern with animal hardwood bedding is its dust content and its possible effects on animals and animal technicians. Previous reports on the quality control of rodent bedding did not specify sample size or shake time for measuring bedding particle size and dust content. These variables could alter particle size analyses. In an effort to more accurately characterize bedding particle size and dust content, 50g and 100g samples of hardwood bedding were shaken for 0.5, 1, 2, 3, 4, or 5 minutes in a portable sieve shaker containing U.S. standard sieves (Nos. 8, 20, 30 and 50) to determine optimum sample size and shake time. Significant differences (P less than 0.05 or greater) were observed in the percent of bedding retained on a No. 8 sieve when 50g and 100g samples were taken for 30 seconds or for 1 minute. Samples shaken for 2 or more minutes did not show any statistical differences in the percent of bedding which was retained on or passed through the different sieves. Major differences occurred in the percent of bedding which was retained or passed through the different sieves, when the shake time was varied from 0.5 to 5 minutes. These results indicated that 0.5 or 1 minute was definitely not enough time to accurately measure bedding particle size and dust content and that the sample size and shake time must be consistent in order to accurately compare the particle size and dust content of different shipments of bedding or to compare bedding from different vendors.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗

Synaptic control of motoneuron excitability in rodents: from months to milliseconds.

1. Motoneurons (MN) shape motor patterns by transforming inputs into action potential output. This transformation, excitability, is determined by an interaction between synaptic inputs and intrinsic membrane properties. Excitability is not static, but changes over multiple time scales. The purpose of the present paper is to review our recent data on synaptic factors important in the dynamic control of MN excitability over time scales ranging from weeks to milliseconds. 2. Developmental changes in modulation of MN excitability are well established. Noradrenergic potentiation of hypoglossal (XII) MN inspiratory activity in rhythmically active medullary slice preparations from rodents increases during the first two postnatal weeks. This is due to increasing alpha 1- and beta-adrenoceptor excitatory mechanisms and to a decreasing inhibitory mechanism mediated by alpha 2-adrenoceptors. Over a similar period, ATP potentiation of XII inspiratory activity does not change. 3. Motoneuron excitability may also change on a faster time scale, such as between different behaviours or different phases of a behaviour. Examination of this has been confounded by the fact that excitatory synaptic drives underlying behaviour can obscure smaller concurrent changes in excitability. Using the rhythmically active neonatal rat brain-stem-spinal cord preparation, we blocked excitatory inspiratory drive to phrenic MN (PMN) to reveal a reduction in PMN excitability specific to the inspiratory phase that: (i) arises from an inhibitory GABAergic input; (ii) is not mediated by recurrent pathways; and (iii) is proportional to and synchronous with the excitatory inspiratory input. We propose that the proportionality of the concurrent inhibitory and excitatory drives provides a means for phase-specific modulation of PMN gain. 4. Modulation across such diverse time scales emphasizes the active role that synaptic factors play in controlling MN excitability and shaping behaviour.

Adenosine Triphosphate↗

Sexual behavior and aggression in male mice: involvement of the vomeronasal system.

Recent observations have implicated the vomeronasal (accessory olfactory) system in the chemosensory control of rodent social behaviors. The purpose of this study was to observe the effects of peripheral vomeronasal organ extirpation on sexual behavior, aggression, and urine marking in male mice. Relative to sham-operated control animals, mice lacking vomeronasal organs displayed significantly reduced levels of copulatory behavior and intermale aggression. Urine marking rates were not reduced. The peripheral removal of the vomeronasal organ resulted in complete bilateral deafferentation of the accessory olfactory bulbs but spared the peripheral input to the main olfactory bulbs as evidenced by the lack of anterograde vomeronasal nerve transport but normal anterograde olfactory nerve transport of intranasally applied horseradish peroxidase. Neither body weights, paired testes weights, nor seminal vesicle weights of mice with vomeronasal system lesions differed significantly from those of control animals. Thus, an intact vomeronasal organ is important for the normal display of sexual behavior and aggression in male mice, and the reductions in these androgen-dependent behaviors following peripheral deafferentation of the vomeronasal system cannot be attributed to a chronic reduction of gonadal hormone secretion.

Aggression↗

Evaluation of two rodenticides in the paddy fields during Samba and Thaladi seasons.

The single dose anticoagulant rodenticide, bromadiolone (0.005%) and the acute rodenticide, zinc phosphide (2%) were evaluated in the paddy fields during Samba and Thaladi seasons for two crop stages, viz. 20 and 40 days after transplantation. Three baiting methods namely, burrow, station and burrow+station were adopted for both rodenticides. Both the rodenticides were exposed for one and two days in the partitioned plots. In the plots with 20 days after transplantation, the two day exposure of both rodenticides in burrow+station baitings during both seasons cleared cent percent rodent population. The cost-benefit ratio of the employed rodenticides favoured zinc phosphide than bromadiolone. Thus, zinc phosphide is deemed to be an economic rodenticide than bromadiolone and it can be suggested for the control of rodent population with two day exposure by burrow+station baiting methods preferably 20 days after transplanted paddy fields in both seasons.

4-Hydroxycoumarins↗

A method for measuring locomotor behavior in rodents: contrast-sensitive computer-controlled video tracking activity assessment in rats.

A newer locomotor activity system for rodents is described. The system consists of a black, ventilated test chamber, internally lighted with a ceiling mounted video camera. The camera's image is transmitted to a contrast-sensitive tracker which maps the point of highest contrast and relays the digitalized coordinates to a PC. Dedicated software stores the information and simultaneously displays a map of the tracked subject. To illustrate the system's utility, results from an experiment are presented using an established behavioral teratogen, phenytoin. Pregnant Sprague-Dawley CD rats were exposed to 0 or 200 mg/kg of phenytoin by gavage on embryonic days 7-18 and the offspring tested in the videotracker activity monitoring device. Phenytoin is known to induce hyperactivity and circling behavior in the offspring. The system revealed that phenytoin-exposed offspring that exhibit neurological impairment were hyperactive compared to controls and the effect was predominantly seen in females. These animals exhibited more section transitions and more central and peripheral activity. When peripheral activity was subdivided into that occurring along corners versus sides, it was found that corner activity represented only a small component of the group differences whereas the side component represented most of the effect. Moreover, phenytoin offspring exhibiting the circling defect were found to display a qualitatively different pattern than noncirclers or controls. Videotracking represents a different approach to the analysis of locomotor activity patterns in experimental animals compared to older methods. Two advantages of this method are higher spatial resolution and not having to pre-specify data capture intervals. Other features are detailed regional movement information and qualitative mapping of ambulatory patterns.

Analysis of Variance↗

Pen and field trials of a new anticoagulant rodenticide flocoumafen against the house mouse (Mus musculus L.).

The efficacy of flocoumafen, a novel anticoagulant rodenticide, was evaluated in feeding tests on confined and free-living populations of house mice (Mus musculus L.). In four pen trials, family groups of laboratory-reared wild mice were conditioned to feeding on plain foods and then offered flocoumafen at 0.005% in pinhead oatmeal bait. All 68 mice, comprising juvenile and adult animals, died within 10 days. Ten field trials were carried out, using the same formulated poison bait, against mice infesting farm buildings. Mean treatment success, estimated from live-capture and mortality data, ranged between 87.1 and 100%. The performance of flocoumafen is compared with that of difenacoum, bromadiolone and brodifacoum used at the same concentration in oatmeal bait. Flocoumafen gave an equally effective but quicker kill of mice. It is concluded that flocoumafen is a promising new rodenticide for the control of M. musculus.

4-Hydroxycoumarins↗

The adoption of management and husbandry procedures by Western Australian pig farmers.

A postal survey was conducted to determine the management and husbandry procedures adopted by a sample of 101 Western Australian pig farmers. Seventy seven replies were received and analysed. Over 80% of farmers vaccinated breeding stock against leptospirosis and erysipelas, 35% vaccinated against parvo virus and 15% vaccinated against Escherichia coli. Most farmers used antibiotics: 72% incorporated them into their pig diets and 60% used parenteral antibiotics. Fifty three per cent of farmers reported that they regularly used a veterinarian. Over 59% of piggeries had another piggery located within 10 km, thus increasing the possibility for disease transmission between herds. It is concluded that there is potential for veterinarians to offer advice to pig farmers, particularly on disease control measures and adoption of technological advances.

Animal Husbandry↗

Food preferences of wild house-mice (Mus musclus L).

The relative acceptance of various plain foods by wild house-mice (Mus musculus L.) was compared in laboratory choice tests. The palatability of glycerine and six oils, each included at 5% in pinhead oatmeal, was compared in a similar manner.The most favoured food was found to be whole canary seed (Phalaris canariensis). Pinhead oatmeal and wheat were also comparatively well accepted. Glycerine, corn oil, arachis oil and mineral oil were more palatable than either olive, linseed or cod-liver oils.The results of the choice tests are considered in relation to the use of poison baits for the control of free-living mice.

Animals↗