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Rinderpest surveillance performance monitoring using quantifiable indicators.

This paper describes an objective system of monitoring the performance of disease surveillance. The system was developed through dialogue with a number of countries in Africa and adopted as part of the Global Rinderpest Eradication Programme of the Food and Agriculture Organization of the United Nations. The performance monitoring system uses a clinical stomatitis-enteritis case definition, an outbreak investigation classification scheme, and a series of eight performance indicators to measure the sensitivity, specificity and timeliness of the surveillance system. Field-testing indicates that the approach is successful when good record-keeping is practiced and highlights the importance of dialogue in helping to ensure that the system is simple and acceptable. The system provides a quantitative measure of the efficacy of national disease surveillance programmes and of the quality of data derived from such programmes for use in international disease control, animal health information exchange and trade risk analysis.

Animals↗

Analysis of structural proteins of measles, canine distemper, and rinderpest viruses.

Serological relationships among measles virus (MV), canine distemper virus (CDV), and rinderpest virus (RV), which constitute morbillivirus subgroup of paramyxoviridae, were investigated by immunoprecipitation and SDS-polyacrylamide gel electrophoresis for their major structural proteins, i.e., hemagglutinin (H), nucleocapsid (NC), fusion (F), and matrix (M) proteins. The molecular weights of the four structural proteins of MV and CDV were confirmed to correspond to those previously reported by several investigators. Structural proteins of RV were analyzed for the first time in the present study and found to have molecular weights of 74,000, 62,000, 44,000, and 40,000 for H, HC, F, and M proteins, respectively. By labeling with glucosamine, the presence of carbohydrate moiety was found in H protein for all the three viruses and in F protein of CDV. The serums from the convalescent animals infected with respective virus disclosed one-way cross pattern depending on the combinations of virus and antiserums, but failed to show the reciprocal cross reactivity. On the other hand, hyperimmune serums to respective virus showed the reciprocal cross-reactivity with the four structural proteins indicating that each of the major structural proteins possesses the antigen common to all three morbilliviruses.

Antigens, Viral↗

Diminished antibody response to rinderpest vaccination in cattle undergoing experimental East Coast fever.

The immunocompetence of cattle undergoing East Coast Fever (ECF) reactions of varying degrees of intensity was evaluated using the neutralising antibody response of these cattle to the vaccine response was found in animals undergoing severe ECF reactions. The results suggest that the massive lymphoid cell involvement of such severe ECF reactions diminishes the immune responsiveness of cattle to rinderpest vaccine.

Animals↗

Development of a thermoresistant tissue culture rinderpest vaccine virus.

The currently used Plowright's tissue culture rinderpest vaccine (RBOK strain) gives full protection and lifelong immunity, but it is highly thermolabile and requires maintenance of cold chain from vaccine production till delivery. Keeping in view the need for a thermostabile vaccine in tropical developing countries with limited refrigeration facilities, we passaged serially the RBOK strain of rinderpestvirus (RPV) at gradually elevated temperature up to 40 degrees C to obtain a thermoresistant RPV (TR-RPV) mutant. The thermoresistance (thermostability) and antigenicity of TR-RPV were compared with those of the vaccine virus by various methods, confirming the acquired properties. Thus, the infectivity titres of the TR-RPV mutant and vaccine virus were determined after incubation for various times at 37 degrees C. Regression analysis indicated that TR-RPV had a half-life of 1.81 hr and a degradation constant of 0.1656, while the parent vaccine virus had a half-life of 1.11 hr and a degradation constant of 0.2686. In capture ELISA with four different monoclonal antibodies (MAbs) to the N protein of RPV, TR-RPV showed a 10-fold higher reactivity with one MAb as compared to the vaccine virus. Although TR-RPV did react also with the other three MAbs, its reactivity was only 4-5 times higher than that of the vaccine virus. A treatment of the virus with Triton X-100 resulted in 2-4 times higher reactivity with the MAbs. The 35S-methionine-labeled vaccine virus-and TR-RPV-infected Vero cell lysates showed 6 polypeptide bands with identical pattern of migration in polyacrylamide gel electrophoresis in the presence of SDS (SDS-PAGE). Radioimmunoprecipitation assay (RIPA) of the TR-RPV and vaccine virus with a rabbit anti-RPV immune serum (RHIS) and bovine anti-RPV hyperimmune serum (BHIS) showed the presence of four identical antigenic proteins, namely H, N, F and M, for both viruses. It can be concluded that TR-RPV has indeed retained the antigenic properties of the parental vaccine virus besides acquiring thermoresistance.

Animals↗

Localization of antigenic sites at the amino-terminus of rinderpest virus N protein using deleted N mutants and monoclonal antibody.

The nucleocapsid (N) protein of rinderpest virus (RPV) is highly conserved, immunogenic, and abundantly expressed during infection. Six antigenic sites (sites A, B, C, D, E and F), defined previously by a competitive binding assay using corresponding monoclonal antibodies (Mabs), have been further localized by immunoassays using deleted N mutants. Five different forms of RPV N protein, containing residues aa 1-79, aa 1-149, aa 1-421, aa 414-525 and aa 1-525, were expressed as glutathione S transferase (GST) fusion proteins (designated as GST-N1-79, GST-N1-149, GST-N1-421, GST-N414-525, and GST-N1-525, respectively) in E.coli BL21 cells. In ELISA using deleted N mutants, Mabs recognizing sites A, B, C, D and E reacted with 3 GST fusion proteins (GST-N1-149, GST-N1-421 and GST-N1-525), indicating that they are located at aa 80-149. Mab recognizing site F reacted with 4 GST fusion proteins (GST-N1-79, GST-N1-149, GST-N1-421 and GST-N1-525), indicating that site F is located at aa 1-79. Identification of the amino-terminal antigenic sites of the N protein would provide antigen basis for developing sensitive and specific diagnostic reagents for RPV, although it remains to be further investigated antigenic sites at the carboxyl-terminus.

Amino Acid Sequence↗

[Control of post-vaccinal immunity against rinderpest after the 1989 and 1990 vaccination campaigns in Ivory Coast].

The Central Laboratory of Animal Pathology of Bingerville (Côte-d'Ivoire) made an evaluation of post vaccinal immunity against rinderpest in 1990 and 1991 after the 1989 and 1990 national vaccination campaigns. The random sampling method was chosen to collect 6,020 sera in 255 places in 1990 and 3,301 sera in 158 places in 1991. ELISA analysis gave a standard positive rate of 82.39% +/- 0.08 in 1990 and 88.26 +/- 0.06% in 1991.

Animals↗

Robert Koch and the rinderpest.

This is an account of the rinderpest outbreak in southern Africa in the closing decade of the 19th century, with special reference to the Transvaal and northern Cape which brought socio-economic disaster in its wake in President Kruger's Zuid-Afrikaansche Republiek. Robert Koch was invited to South Africa by the Cape Government to investigate the cattle plague and to find a cure. His stay in Kimberley was sponsored by the De Beer's Company and the Kimberley Diamond Fields Advertiser gave regular, sometimes dramatic, reports of the progress of the work. Although doubt seems to have existed in some quarters as to the complete success of Robert Koch's vaccine, the consensus appears to be that it was efficacious.

Animals↗

Detection of rinderpest antigen by latex agglutination test.

A slightly modified latex agglutination test was applied for detection of rinderpest antigen. The antigen was added to sensitized latex particles in the presence of hyperimmune antiserum to facilitate agglutination. Out of 129 samples tested by latex agglutination (LA), solid phase aggregation of coated erythrocytes (SPACE), reverse phase passive haemagglutination (RPHA) and counter immunoelectrophoresis (CIE) test, 86.0, 86.8, 84.4 and 79.8 per cent, respectively, were found positive.

Antigens, Viral↗

Microtitre techniques for the assay of rinderpest virus and neutralising antibody.

Microtitre techniques were compared with conventional tube techniques for their ability to assay rinderpest virus and neutralising antibody to the virus. The microtitre technique was as sensitive and reliable for assaying the virus as the recommended tube technique, using cell suspensions. Both of these methods, however, were less sensitive than tube titrations on preformed cell monolayers. The microtitre test was as sensitive as the tube test for detecting and assaying virus neutralising antibody and more robust in that it was less sensitive to variations in virus dose.

Animals↗

[Evaluation of the vaccinal protection against rinderpest in Cameroon. I. Province of Adamaoua].

As a part of the panafrican rinderpest campaign, a serological survey of 5,720 samples was undertaken in the Adamaoua province of Cameroon. The herd immunity level varied from 60 to 73% in the four departments investigated. Vaccination of all animals from the age of six months, replacement of vaccination equipment, restoration of crushes, and use of magnesium sulfate, or as a substitute, saline for lyophilized vaccine reconstitution are recommended.

Animals↗

Detection of anti-DNA antibody responses in rabbits infected with rinderpest virus by ELISA.

ELISA was standardized to detect anti-DNA antibodies (ADAbs) in sera of rabbits infected with rinderpest virus (RPV). These antibodies were found in late (28th and 36th day post inoculation (dpi)) sera of rabbits infected with rabbit virulent lapinized RPV, but not in early (7th dpi) sera of the same rabbits. ADAbs were not found either in early or late sera of rabbits infected with attenuated RBOK strain of RPV. Rabbit anti-RPV hyperimmune serum was also found to be positive for ADAbs. Detection of these antibodies by ELISA proved DNA to be one of the target antigens for antinuclear antibodies induced in RPV infection.

Animals↗

Protective vaccination of ferrets against canine distemper with recombinant pox virus vaccines expressing the H or F genes of rinderpest virus.

OBJECTIVE: To investigate the ability of rinderpest virus (RPV) antigens, expressed in pox virus vectors, to protect against canine distemper virus (CDV) infection in ferrets. ANIMALS: Ferrets (Mustela putorius; n = 27) with no previous exposure to CDV. PROCEDURE: Ferrets were inoculated intradermally with recombinant vaccinia viruses expressing the H gene of RPV, the F gene of RPV, the H and F genes of RPV, or fowlpox virus recombinant expressing both genes. Two ferrets were vaccinated s.c. with CDV vaccine as positive controls, and 1 group was left unvaccinated as a negative control. Blood was obtained from ferrets biweekly; antibody titer to RPV was detected by ELISA, and CDV antibody titer was measured by serum neutralization testing and ELISA. RESULTS: Partial protection was seen in all groups, with vRVFH vaccination being the most protective (60%). CONCLUSIONS AND CLINICAL RELEVANCE: A single inoculation with a vaccinia virus expressing the H and F genes of RPV was able to protect 60% of the vaccinated ferrets challenge exposed with a high dose of CDV. These results indicate the ability of RPV antigens expressed by vaccinia virus to protect ferrets against a related morbillivirus. Further, they document the safety and efficacy of a recombinant vaccinia virus vaccine for ferrets. Such vaccines may be useful given the susceptibility of ferrets to CDV and the problem of maternal antibody interfering with vaccination of young animals.

Animals↗

Rinderpest in South Africa--100 years ago.

To commemorate the centenary of the outbreak of rinderpest in South Africa, the historical events leading up to and following this major epidemic are recounted. Its impact on livestock and human populations, as well as initial and eventual successful attempts to control it are discussed.

Animals↗