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At least 289 records · Page 16Linked to original sources
PubMed1989

Shoot culture dynamics of six Populus clones.

Shoot tips of five genotypically diverse Populus clones, P. alba x P. grandidentata 'Crandon,' P. nigra 'Betulifolia' x P. trichocarpa, P. nigra x P. laurifolia 'Strathglass,' P. maximowiczii x P. trichocarpa 'Androscoggin' and P. deltoides x P. nigra 'Eugenei,' were collected from hardwood cuttings, sterilized,and established in vitro. Stable shoot cultures were obtained from all clones except P. deltoides x P. nigra 'Eugenei'. The four poplar clones that formed stable shoot cultures together with a previously established P. tremula 'Erecta' clone were placed as two-node explants on either Murashige and Skoog medium or Woody Plant Medium containing benzyladenine to determine the rate of shoot multiplication, shoot growth and other responses of the clones. All five poplar clones showed rapid shoot multiplication when cultured in the presence of 0.4-1.0 microM benzyladenine on Murashige and Skoog medium, although P. tremula 'Erecta' produced a greater number of healthy shoots when grown on Woody Plant Medium. Individual shoot growth of all clones was more vigorous when the medium contained 0-0.1 microM benzyladenine, and 100% of such shoots rooted ex vitro.

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PubMed1990

Validation of photosynthate production in ECOPHYS, an ecophysiological growth process model of Populus.

A model of photosynthate production is the central component of a larger whole-tree ecophysiological growth process model for Populus (ECOPHYS). This photosynthesis model was validated by comparing predicted photosynthate production values for individual leaves and the total tree with hourly field measurements collected on four days spaced throughout a growing season. Simulated trees had identical numbers of leaves and leaf areas as the sample trees studied in the field, and hourly weather data collected on the plantation site were supplied as a model input. Total production for the four sample days ranged between 200 and 4900 mg CO(2) tree(-1) day(-1). Model predictions of total daily photosynthate production were within 12% of the observed rates for three of the four sampling days. Diurnal variations in stomatal conductance and ambient CO(2) concentrations and seasonal variations in area leaf weight were the primary sources of error. Total leaf area, proportion of sunlit leaf area, and photosynthetic efficiency were the most important factors influencing carbon dioxide exchange rates.

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PubMed1990

Effects of leaf display on light interception and apparent photosynthesis in two contrasting Populus cultivars during their second growing season.

Effects of the contrasting leaf display of poplar cultivars Eugenei (Populus x euramericana) and Tristis (P. tristis x P. balsamifera) on light interception and photosynthesis were studied in the second year of growth in an irrigated plantation near Rhinelander, Wisconsin, USA (lat. 45 degrees N). Leaves on the current terminal (CT) and on proleptic branches were measured between 0900 and 1500 h on five clear days from June to September 1980. Leaf orientation-based differences between these cultivars were evident as the second growing season progressed and the crowns of the trees in the plantation grew together. Leaves of Eugenei are erectophile or tilted from the horizontal. In this cultivar light penetrated throughout the crown; many leaves on the lowest branches were illuminated as fully as those on the upper CT and had higher photosynthetic rates than equivalent leaves in Tristis. However, by early September many of the lower branches on Eugenei trees had abscised. In the planophile Tristis, adaxial photon flux densities (PPFD) of leaves on the lower portion of the CT and on branches were only a fraction of those measured on the upper CT. This pattern became more extreme as the season progressed. Few of the lower branches of Tristis abscised during the growing season. Photosynthesis rates, especially on a whole-leaf basis, were closely related to incident PPFDs in both cultivars. The ecological significance of these results are discussed, as well as the hypothesized effect of leaf inclination on crop productivity.

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PubMed1991

Carbon dioxide enrichment accelerates the decline in nutrient status and relative growth rate of Populus tremuloides Michx. seedlings.

Changes in growth dynamics and mineral nutrient concentrations were measured in Populus tremuloides Michx., trembling aspen, grown for 100 days following germination in atmospheres containing 350 or 750 microl l(-1) CO(2). Seedlings were fertilized with nitrogen (N) at concentrations of 15.5 mM (high-N), 1.55 mM (medium-N), or 0.155 mM (low-N). Initially, relative growth rates were enhanced by CO(2) enrichment in each N regime, but the effects did not persist. In plants grown in high-N or medium-N, foliar concentrations of Ca and Mg decreased in response to CO(2) enrichment. During the 100-day study, whole-plant concentrations of N and P decreased in all treatments. The decreases in mineral nutrient concentrations over time were accelerated in CO(2)-enriched plants and accompanied the disappearance of the CO(2)-induced growth enhancement. It is concluded that the depression of relative growth rates often associated with long-term CO(2) enrichment of plants may result from decreases in plant nutrient status.

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PubMed1991

Clonal variation of Populus tremuloides responses to diurnal drought stress.

We have developed an automated microprocessor controlled system for subjecting hydroponically grown plants to drought. Pumps and valves were used to move nutrient solutions into and out of a system of culture vessels in a growth chamber to provide periods of drought. Drought conditions were obtained by exposing the roots of hydroponically grown clones of aspen, Populus tremuloides Michx., to air in culture vessels temporarily emptied of nutrient medium. Over a 3-week period, the daily duration of drought was increased from 0 to 6 h. During this period, the plants became increasingly tolerant to drought, as shown by a decreasing propensity to wilt. All three clones sustained diurnal drought periods of 6 h for up to 5 weeks without detectable deterioration of health. Typical drought stress symptoms were observed including inhibition of growth, increased tissue amino acid content, and decreased water, solute, and turgor potentials in young leaves. In all clones, control plants had leaf water potentials between -1.0 and -1.6 MPa, whereas leaf water potentials of drought-treated plants were significantly lower, ranging from -1.7 to -3.0 MPa. Only one of the clones showed a significant decrease in leaf solute potential in response to drought. The decrease in leaf solute potential paralleled the decrease in water potential resulting in no significant difference in turgor potential. The other two clones had nonsignificant decreases to more negative leaf solute potentials under drought conditions resulting in significantly lowered turgor potentials. Leaf water potentials, solute potentials, and turgor potentials of the drought-treated plants returned to control values within two hours after rewatering. The growth inhibitions observed could not have been the consequence of loss of turgor. These results demonstrate genetic differences among aspen clones in water relations responses to drought.

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PubMed1993

Modification of Rubisco and Altered Proteolytic Activity in O3-Stressed Hybrid Poplar (Populus maximowizii x trichocarpa).

Exposing hybrid poplar (Populus maximowizii x trichocarpa) plants to ozone (O3) resulted in an acceleration of the visual symptoms of senescence and a decrease in the activity and quantity of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco). Whole plants, crude leaf extracts, and isolated intact chloroplasts of hybrid poplar clone 245 were used to test the hypothesis that O3-induced structural modifications of Rubisco affect the activity of this key photosynthetic enzyme. Proteolytic activity, per se, could not account for losses in Rubisco; acidic and alkaline protease activities declined or were unaffected in foliage of O3-treated poplar saplings. In vitro treatment of leaf extracts with O3 decreased total Rubisco activity and binding of the enzyme's transition-state analog, 2-carboxyarabinitol bisphosphate. Additionally, O3 increased the loss of Rubisco large subunit (LSU) when extracts were incubated at 37[deg]C. Treatment of isolated intact chloroplasts with O3 accelerated both the loss of the 55-kD Rubisco LSU and the accumulation of Rubisco LSU aggregates, as visualized by immunoblotting. The time-dependent modification in Rubisco structure was the primary response of the isolated organelles to O3 treatment, with little proteolytic degradation of the LSU detected.

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PubMed1993

Metabolism of Transpired Ethanol by Eastern Cottonwood (Populus deltoides Bartr.).

Ethanol has previously been shown to be present in the xylem sap of flooded and nonflooded trees. Because of the constitutive presence of alcohol dehydrogenase in the mature leaves of woody plants, we hypothesized that the leaves and shoots of trees had the ability to metabolize ethanol supplied by the transpiration stream. 1-[14C]Ethanol was supplied to excised leaves and shoots of eastern cottonwood (Populus deltoides Bartr.) in short- and long-term experiments. More than 99% of the radiolabel was incorporated into plant tissue in short-term experiments, with more than 95% of the label remaining in plant tissue after 24 h. In all experiments, less than 5% of the label was transpired as ethanol and less than 1% was emitted as CO2. In excised leaf experiments, less than 0.5% of the radiolabel escaped from the leaf. Fifty percent of the label was incorporated into the petioles of excised leaves; 56% was incorporated into the stems of excised shoots. Very little label reached the leaf mesophyll cells of excised shoots, as revealed by autoradiography. Radiolabel appeared primarily in the water- and chloroform-soluble fractions in short-term experiments, whereas in long-term experiments, label was also incorporated into protein. These results demonstrate that the leaves and stems of trees appear to have substantial ability to scavenge ethanol from the transpiration stream, allowing efficient recovery of ethanol produced elsewhere by hypoxic tissues. When labeled ethanol was supplied to excised petioles in a 5-min pulse, 41% of the label was incorporated into organic acids. Some label was also incorporated into amino acids, protein, and the chloroform-soluble fraction, with very little appearing in neutral sugars, starch, or the insoluble pellet. Labeled organic acids were separated by high performance liquid chromatography and were composed of acetate, isocitrate, [alpha]-ketoglutarate, and succinate. There was no apparent incorporation of label into phosphorylated compounds. We conclude that, in higher plants, ethanol is metabolized to acetaldehyde and then to acetate by alcohol and aldehyde dehydrogenases, and then into general metabolism.

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PubMed1993

Physiological and Environmental Requirements for Poplar (Populus deltoides) Bark Storage Protein Degradation.

In poplar (Populus deltoides Bartr. ex Marsh), a 32-kD bark storage protein (BSP) accumulates in the bark during autumn and winter and declines during spring shoot growth. We investigated the physiological and environmental factors necessary for the degradation of poplar BSP. Poplar plants were exposed to short-day (SD) photoperiods for either 28 or 49 d. Plants exposed to short days for 28 d formed a terminal bud but were not dormant, whereas exposure to short days for 49 d induced bud dormancy. BSP accumulated in bark of plants exposed to both SD treatments. The level of BSP declined rapidly when nondormant plants were returned to long days. BSP levels did not decline in dormant plants that were exposed to long-day (LD) conditions. If dormant plants were first treated with either low temperatures (0[deg]C for 28 d) or with 0.5 M H2CN2 to overcome dormancy and then returned to long days, the level of BSP declined. Removal of buds from non-dormant or dormant plants in which dormancy had been overcome inhibited the degradation of BSP in LD conditions. BSP mRNA levels rapidly declined in plants exposed to long days, irrespective of the dormancy status of the plants or the presence or absence of buds. These results indicate that the buds of poplars are somehow able to communicate with bark storage sites and regulate poplar BSP degradation. These results further support an association of BSP mRNA levels with photoperiod because short days stimulate BSP mRNA accumulation, whereas long days result in a decline of BSP mRNA abundance.

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PubMed1971

Chlorophyll, Ribulose-1,5-diphosphate Carboxylase, and Hill Reaction Activity in Developing Leaves of Populus deltoides.

The synthesis of chlorophyll and ribulose diphosphate carboxylase as well as the development of Hill reaction activity were followed in expanding Populus deltoides leaves and related to photosynthetic patterns. Total chlorophyll, which was not correlated with photosynthetic rate in expanding leaves, decreased slightly with age in very young leaves, due to a decrease in chlorophyll b, but then increased linearly. The ratio of chlorophyll a to b, which rose sharply in young leaves, was highly correlated with the onset of net photosynthesis. Hill reaction activity was very low in young leaves and did not increase significantly until leaves were about half expanded. Ribulose diphosphate carboxylase activity increased in a sigmoid fashion with leaf ontogenesis and closely paralleled development of the photosynthetic system. The study demonstrates the importance of chlorophyll a and Calvin cycle enzyme synthesis to photosynthetic development in expanding leaves.

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PubMed1972

Photophosphorylation and Carbon Dioxide Fixation by Chloroplasts Isolated from Populus deltoides.

A system has been developed for the isolation of photosynthetically active chloroplasts from leaves of Populus deltoides. A high proportion of the chloroplasts appeared intact. The maximum rates of different photosynthetic processes were as follows: CO(2) fixation 3.5 micromoles per milligram chlorophyll per hour, noncyclic ATP synthesis 10 micromoles per milligram chlorophyll per hour, and cyclic ATP synthesis 300 micromoles per milligram chlorophyll per hour.

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PubMed1972

Decrease of Glucose 6-Phosphate and 6-Phosphogluconate Dehydrogenase Activities in the Xylem of Populus gelrica on Budding.

The activities of glucose 6-phosphate and 6-phosphogluconate dehydrogenases, transketolase, phosphoglucose isomerase, and fructose 6-phosphate kinase were studied in extracts of wintering poplar (Populus gelrica) xylem. The xylem of wintering poplar showed high levels of transketolase, glucose 6-phosphate, and 6-phosphogluconate dehydrogenases. On recommencement of growth, the two dehydrogenase activities decreased. The three remaining enzymes appeared to be unchanged. In spring and early summer, glucose 6-phosphate dehydrogenase of the xylem was extremely low. On the other hand, 6-phosphogluconate dehydrogenase, which also became lower during the metabolic shift from winter to spring, was readily detected, and was several times higher than glucose 6-phosphate dehydrogenase throughout the year. The low dehydrogenase activities lasted into late October and then appeared to resume their original activity. A shift of metabolism at the beginning of growth was also observed by measuring the amount of sugar phosphates, soluble amino acids and amides, and proteins in the xylem. In contrast to the decrease of the two dehydrogenases and soluble proteins at the time of budding, incorporation of lysine-U-(14)C into the xylem protein ramained constant. A method to transfuse radioactive compounds into a section of stem was described.

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PubMed1973

Functional and Structural Changes in Senescing Populus deltoides (Bartr.) Chloroplasts.

Chloroplasts isolated from Populus deltoides leaves were used to study age-dependent changes in the rate of cyclic photophosphorylation. Single leaves were used to measure CO(2) fixation by leaf discs, chlorophyll concentration, and ATP synthesis. The ability of chloroplasts to synthesize ATP diminished steadily from the time of full leaf expansion, regardless whether the results are expressed on a leaf area or chlorophyll basis. This decline in the rates of ATP synthesis was paralleled by the decline in the rate of CO(2) fixation. The results suggest that the efficiency of the membrane-bound ATP synthesizing system declines with age.

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PubMed1975

Incorporation of C-Photosynthate into Protein during Leaf Development in Young Populus Plants.

Gas exchange and protein metabolism were studied in expanding, mature, and near-senescent leaves of young clonal Populus x euramericana cv. Wisconsin-5 plants. Dark respiration, CO(2) evolution in the light, and CO(2) compensation concentrations were highest in unexpanded leaves but declined markedly as leaves matured and aged. Net photosynthesis was highest in nearly mature leaves. Fresh weight continued to increase after leaf expansion was complete, whereas soluble protein levels declined. Changes in the distribution of photosynthetically incorporated (14)C indicated that a high level of protein synthesis and rapid formation of structural components occurred only in expanding leaves. Protein turnover was slight in expanding leaves but was substantial after leaves were mature. Expanding leaves synthesized predominantly fraction I protein (ribulose diphosphate carboxylase). However, formation of this protein from photosynthate was slight once leaves matured.

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PubMed1976

The Occurrence of Peroxide in a Perennial Plant, Populus gelrica.

A large amount of peroxide was found in twigs of poplar, Populus gelrica, which was grown in the field under natural conditions. The peroxide found in xylem and living bark was about 1.2 and 0.5 mumoles per gram dry weight sample, respectively, and served as a substrate both for catalase and cytochrome c peroxidase.

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PubMed1976

Physiological Characteristics of Photosynthesis and Respiration in Stems of Populus tremuloides Michx.

The physiological responses of 6- to 8-year-old aspen (Populus tremuloides Michx.) stems to temperature, light, and CO(2) concentration were investigated in the field throughout the year using infrared CO(2) analysis. Light response studies showed that the rate of gross photosynthesis was linear from 0 to 400 ft-c (0 to 1.6 mw/cm(2) of 400-700 nm) with light saturation being reached between 800 to 1400 ft-c (3.2 to 5.6 mw/cm(2) of 400-700 nm). At this light intensity, the respiratory CO(2) loss was reduced to 10 to 15% of dark rates. Net photosynthetic CO(2) uptake was not observed even at intensities as high as 3400 ft-c (13.6 mw/cm(2) of 400-700 nm). The light response curve was similar for both winter and summer stems.During summer months, the respiratory and photosynthetic rates of the aspen stem increased with temperature at a near constant rate between 5 and 35 C. For winter stems, the gross photosynthetic rate increased in a pattern similar to the dark respiratory rate as the temperature rose from 3 to 17 C. Below 0 C and above 17 C, however, the gross photosynthetic rate fell off in relation to the respiratory rate so that the per cent of CO(2) reassimilated decreased from 75% to less than 50%. Measurable bark photosynthetic activity was not observed below -3 C.The gross photosynthetic rate of stems was not affected when the gas passing through the cuvette contained concentrations of CO(2) ranging from 0 to 580 mul CO(2)/l air.

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PubMed1976

Diurnal and Seasonal Patterns of Photosynthesis and Respiration by Stems of Populus tremuloides Michx.

The photosynthetic and respiratory rates of 5- to 7-year-old aspen stems (Populus tremuloides Michx.) were monitored in the field for 1 year to determine the seasonal patterns. The stem was not capable of net photosynthesis, but the respiratory CO(2) loss from the stem was reduced by 0 to 100% depending on the time of year and the level of illumination as a result of bark photosynthesis. The monthly dark respiratory rate ranged from 0.24 mg CO(2)/dm(2). hr in January to a maximum 7.4 mg CO(2)/dm(2). hr in June. Individual measurements ranged from 0.02 mg CO(2)/dm(2). hr in February to 12.3 mg CO(2)/dm(2). hr in June. Gross photosynthesis followed a pattern similar to the dark respiratory rate. The mean monthly rate was highest in June (1.65 mg CO(2)/dm(2). hr) and lowest in December (0.02 mg CO(2)/dm(2). hr). Individual measurements ranged from 0.0 mg CO(2)/dm(2). hr in winter to 5.5 mg CO(2)/dm(2). hr in July.Winter studies showed that stem respiration continued down to -11 C, the coldest temperature during this study. Upon warning to -3 C, the dark respiratory rate showed a sudden sharp increase (7- to 12-fold) which required many hours to return to normal levels. No measurable photosynthesis occurred below -3 C. Between -3 and 0 C, the maximal photosynthetic rate was reduced to less than 50% of the respiratory rate, but increased to 89% between 5 to 10 C.On a yearly basis, bark photosynthesis in P. tremuloides reduced the stem respiratory CO(2) loss by 28.7% on a daytime basis and an estimated 16 to 18% on a 24-hour basis.

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PubMed1978

Quaking and Gas Exchange in Leaves of Cottonwood (Populus deltoides, Marsh.).

Cottonwood (Populus deltoides, Marsh.) leaves are amphistomatous and have an adaptation in their petiole which allows them to oscillate in wind. A possible function of these oscillations in enhancing gas exchange was studied.Cottonwood leaves were found to oscillate in the presence of wind velocities frequently encountered in nature. A pressure differential across the leaf was shown to result in bulk flow of air through that leaf. Oscillating a cottonwood leaf at frequencies found to occur in nature was found to increase the rate of O(2) flux through the leaf. The measured changes in boundary layer resistances during oscillations were found to be insufficient to account for the increased O(2) flux. Thus, the bulk flow of air through an oscillating cottonwood leaf results in a decreased total resistance which is typically 25% less than that of a still leaf.

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PubMed1981

Stomatal Conductance and Sulfur Uptake of Five Clones of Populus tremuloides Exposed to Sulfur Dioxide.

Plants of five clones of Populus tremuloides Michx. were exposed to 0, 0.2 or 0.5 microliter per liter SO(2) for 8 hours in controlled environment chambers. In the absence of the pollutant, two pollution-resistant clones maintained consistently lower daytime diffusive conductance (LDC) than did a highly susceptible clone or two moderately resistant clones. Differences in LDC among the latter three clones were not significant. At 0.2 microliter per liter SO(2), LDC decreased in the susceptible clone after 8 hours fumigation while the LDC of the other clones was not affected. Fumigation with 0.5 microliter per liter SO(2) decreased LDC of all five clones during the fumigation. Rates of recovery following fumigation varied with the clone, but the LDC of all clones had returned to control values by the beginning of the night following fumigation. Night LDC was higher in the susceptible clone than in the other clones. Fumigation for 16 hours (14 hours day + 2 hours night) with 0.4 microliter per liter SO(2) decreased night LDC by half. Sulfur uptake studies generally confirmed the results of the conductance measurements. The results show that stomatal conductance is important in determining relative susceptibility of the clones to pollution stress.

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