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Quantitative electron microscopy has been used to study the number of callosal axons in the corpus callosum of normal and hypothyroid rats during postnatal development. At birth, the normal corpus callosum contains 4.4 x 10(6) axons. This number increases to 11.4 x 10(6) by 5 days of age (P5) and then, in contrast to cats and primates, remains constant until at least P60, the oldest age examined. The number of axons in the corpus callosum of hypothyroid animals is not significantly different from the values observed in normal rats at all ages studied, although the callosal axons of hypothyroid rats remain structurally immature. As extensive elimination of callosal axons has been shown to occur in normal rats past P5, we conclude that new callosal processes grow through the corpus callosum past this age that compensate numerically for the loss. Moreover, as the number of callosally projecting neurons seems to be higher in hypothyroid rats than in normal controls, it seems that the constant axon number derives from more parent neurons, and thus that there are more axon collaterals per callosal neuron in a normal animal than in a hypothyroid one. Taken together, these data indicate that although hypothyroidism does not alter the total number of callosally projecting axons, it interferes with the normal processes that define or sculpt the projection fields, thereby leading to a numerically normal projection with abnormal topography.
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Down syndrome (DS) thymocytes have a markedly diminished proportion of cells expressing high levels of the alpha, beta T cell receptor (TCR alpha, beta) and the associated CD3 molecule. Thus, we examined the surface expression of TCR alpha, beta and CD3 as well as TCR gamma, delta, CD4, CD8, CD16, and CD45RA on peripheral blood lymphocytes (PBL) from 13 noninstitutionalized subjects with DS and 13 closely age-matched sibling controls using immunofluorescence and flow cytometry. DS PBL expressed high surface levels of TCR alpha, beta and CD3, but, as compared to controls, they had a lower proportion of cells expressing TCR alpha, beta (61% vs. 68%, respectively; P less than or equal to 0.05). Moreover, the absolute number of TCR alpha, beta+ cells was considerably lower for DS subjects than for controls (1634 +/- 229 vs. 2763 +/- 530, respectively; P less than or equal to 0.05). DS subjects had a markedly higher proportion of cells expressing TCR gamma, delta than did the controls (12% vs. 7%, respectively; P less than or equal to 0.02). In addition, DS subjects had a lower proportion of CD4+CD45RA+ cells than controls (22% vs. 35%, respectively; P less than or equal to 0.02), representing naive T cells which have recently emigrated from the thymus. The imbalance in the proportions of T cell subpopulations we have observed in DS PBL may contribute to the increased susceptibility to infection associated with DS and may represent a diminished efficiency in the production of newly differentiated T cells by the DS thymus.
A two-color immunofluorescent analysis indicated that dendritic cells (DCs) in the human axillar lymph nodes (ie, lymph nodal DCs (LnDCs)) can be classified into three subsets. The first subset consists of CD1a+/CD86(- or dim)/CD83(- or dim) nondendriform DCs found mainly in lymph sinuses, the second is of CD1a-/CD86+/CD83+ dendriform DCs scattered in normal T zones, and the third is of large CD1a(bright)/CD86+/CD83+ dendriform DCs occasionally found in hyperplastic T zones. A three-color flow cytometric analysis, immunoperoxidase staining, and electron microscopic observation indicated that the majority of LnDCs corresponded to the first subset, which showed distinctive characteristics of DCs but did not fulfill the ultrastructural criteria for interdigitating reticulum cells (IDCs) and did not contain Birbeck granules. When LnDCs were cultured for 7 days, they became large CD1a(dim)/CD86+/CD83+ dendriform cells, which formed large complexes with many T cells and exhibited distinctive ultrastructural features of interdigitating reticulum cells. LnDCs cultured in the presence of granulocyte/macrophage colony-stimulating factor became markedly larger CD1a(bright)/CD86+/CD83+ dendriform cells forming large complexes with numerous T cells. These findings suggest that cells of the first subset represent immature LnDCs just migrating from epidermis, those of the second subset represent interdigitating reticulum cells, and those of the third subset represent interdigitating reticulum cells probably stimulated with certain immunostimulatory cytokines such as granulocyte/macrophage colony-stimulating factor. It is also suggested that either the second or the third subsets of LnDCs are derived from the first subset.
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This study was designed to evaluate the role of follicular cells in the maintenance of meiotic arrest (germinal vesicle [GV] stage). Bovine ovaries were obtained from a slaughterhouse. Ten oocytes were selected and cocultured for 24 h with two follicular hemisections in a 50-microliters drop of TCM-199 supplemented with 10% fetal calf serum. Follicular hemisections were separated into cellular components--granulosa, theca interna, and theca externa. When the oocytes were cocultured in physical contact with granulosa cells, 36% were maintained in the GV stage. However, the percentage of oocytes in the GV stage was significantly increased (p < 0.05) when the theca interna layer associated with (86%) or not with (78%) granulosa cells was used rather than the hemisection consisting of all three follicular cell layers (57%). When we cocultured oocytes without direct contact, using theca interna associated with granulosa cells, only 35% of the oocytes were maintained in the GV stage. Conditioned medium obtained after culture with two follicular hemisections also maintained a high percentage of oocytes in the GV stage when it was replenished every 4 h. An additional culture of 12 h with fresh unconditioned medium resulted in 95% meiotic resumption. In conclusion, these results suggest that the maintenance of bovine oocytes in the GV stage is influenced by theca cells, independent of contact, and that the oocytes can be maintained by conditioned medium from follicular hemisections.
This study was undertaken to assess the role of follicular cells in the maintenance of meiotic arrest (germinal vesicle [GV] stage). Bovine cumulus-oocyte complexes (COC) were obtained by puncture of ovaries collected at a slaughterhouse. Different monolayers of follicular cells--granulosa cells, theca interna, theca externa, or both types of theca cells together--were cultured in 24-well plates with 1 ml of TCM-199 supplemented with 10% fetal calf serum. Theca cells were obtained by digesting theca layers with collagenase. The medium was renewed 48 h before coculturing selected COC with confluent follicular cell monolayers. Oocytes were maintained in GV stage when cocultured for 12 h directly on monolayers of theca cells. The percentage of oocytes in GV stage was not significantly different between treatments using different types of theca cells (51-66%). Whether COC were cocultured in contact or not in contact with theca cells, the percentage of GV stage was similar (61%). The reversibility of this inhibition was high (85%). However, granulosa cells did not exert meiotic arrest (10%). When oocytes were denuded of their cumulus cells and cocultured with theca cells, only 3% were maintained in GV stage; 59% were maintained in GV stage when COC were not removed before culture. This data provided evidence of the essential role of cumulus cells in maintaining GV stage. In conclusion, we have demonstrated that theca cells, in vitro, maintained bovine oocytes in meiotic arrest. The inhibitory factor(s) produced by theca cells is soluble in the medium and acts through the cumulus cells.
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