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At least 289 records · Page 16Linked to original sources
PubMed1994

Foodhandler-associated Salmonella outbreak in a university hospital despite routine surveillance cultures of kitchen employees.

OBJECTIVE: To describe an outbreak of salmonella food poisoning that probably was due to contamination of mashed potatoes by a foodhandler, which occurred despite a policy for routine surveillance stool cultures of kitchen employees. DESIGN: A case control study of 223 individuals who ate the lunch meal on September 23, 1989, at the Jordan University Hospital (JUH) cafeteria. SETTING: Tertiary care university hospital in Amman, the capital of Jordan. PATIENTS: Individuals who developed loose stool or vomiting 6 to 72 hours after eating the lunch meal of September 23, 1989, at the JUH cafeteria. RESULTS: Of 619 individuals, 183 fit the case definition (attack rate, 19.6%); 150 were employees, 26 were inpatients, and seven were visitors. Twelve other employees became sick 4 to 6 days later and probably were infected secondarily. The incubation period ranged from 16 to 72 hours in 183 instances. Symptoms included diarrhea (88%), fever (71%), abdominal pain (74%), dehydration (34%), and bloody stool (5%). Eighty-four were hospitalized. Cultures of eight food items were negative, but stool culture on 90 of 180 patients and 11 of 61 kitchen employees yielded Salmonella enteritidis group D. A cohort study of 223 individuals revealed a food-specific attack rate of 72% for the steak and potato meal and 18% for the rice and meat meal (RR, 4; CI95, 2.62 to 6.24; P < 0.01). Stratified analysis of the steak and potato meal revealed that the potatoes were implicated most strongly (RR, 1.93; CI95, 1.42 to 2.64; P < 0.01). Cultures were obtained from all kitchen employees, and 11 of 61 grew Salmonella enteritidis group D. One asymptomatic, culture-positive employee prepared the mashed potatoes on September 23. All of these employees had negative stool cultures 3 months earlier. CONCLUSION: This outbreak probably was caused by massive contamination of mashed potatoes by the contaminated hands of the foodhandler. Routine stool culture of foodhandlers is not cost-effective and should not be used as a substitute for health education and proper hygienic practices.

Bacteriological Techniques↗
PubMed2002

Quantitative and qualitative feed restriction on growth characteristics of male broiler chickens.

Four experiments were conducted to evaluate the quantitative and qualitative feed restriction on the performance of male broiler chickens. In the first experiment, broilers fed identical pelleted diets were feed-restricted from 5 to 42 d by giving 95, 90, or 85% of the feed consumed ad libitum by control birds the previous day. In a second experiment, broilers were fed pellet or mash diets from 1 to 7 wk. In Experiments 3 and 4, broilers were feed-restricted to 90% of the ad libitum intake of a control group during different intervals. Results from Experiment 1 indicated that live body weight at 42 d was significantly different between unrestricted and restricted broilers (P < 0.01). A reduction in mortality was noted when feed intake was reduced (P < 0.05). In the second experiment, broilers fed mash had lower body weights and breast meat yields at 42 and 49 d (P < 0.05). Cumulative mortality at 42 and 49 d was reduced in broilers fed mash (P < 0.05). Results from Experiment 3 indicated that body weight and mortality were not significantly different (P > 0.05) at 42 d across treatments. There was a significant (P < 0.01) linear improvement on feed conversion at 42 d due to duration of feed restriction. Breast meat yield at 42 d was less in broilers subjected to mild feed restriction (P > 0.05) (Experiments 3 and 4). Broilers appeared able to recover from mild feed restriction, and there was always an associated reduction in mortality.

Animal Feed↗
PubMed2005

Effects of qualitative feed restriction during rearing on the performance of broiler breeders during rearing and lay.

To prevent health and fertility problems associated with excessive weight gain, broiler breeders are severely feed restricted during rearing, which may affect welfare. We compared the effects of an experimental ad libitum feeding regimen based on qualitative restriction of food intake with conventional quantitative food restriction on the performance of female broiler breeders during rearing and lay. During rearing up to 20 wk of age, control birds were fed restricted amounts of standard broiler breeder mash once daily. Experimental birds had ad libitum access to the same standard mash mixed with 400 g of oat hulls/kg of feed and increasing concentrations of Ca propionate, an appetite suppressant. Mean total mash intake during rearing was 8.12 kg and did not differ between treatments. Both control and experimental birds showed an almost linear growth curve, treatment mean body weights were always within 100 g of the target weight line, and treatments did not differ for body weight uniformity. Groups were subjected to the same quantitative feed restriction from during lay. Feeding regimen during rearing did not affect number of eggs produced, egg weight, or egg quality up to 46 wk of age. We concluded that it may not be necessary to subject chicks to severe quantitative feed restriction to achieve desirable growth curves and body weight uniformity during rearing. Qualitative restriction of feed intake can achieve desirable growth curves in ad libitum fed chicks during rearing, and such a feeding regimen does not have negative effects on hen performance during lay.

Aging↗
PubMed2006

Histological intestinal recovery in chickens refed dietary sugar cane extract.

Sugar cane extract (SCE), the residue after removing glucose, fructose, and sucrose from sugar cane juice, has growth-promoting, antistress, and immunostimulation effects. The objective of this study was to investigate the effects of refeeding dietary SCE on recovery of BW and intestinal histology after withdrawing feed from chickens. Forty-eight male Sanuki Cochin chickens were assigned randomly to 6 treatments and 8 replicates in a completely randomized design. The 6 treatments were intact control chickens fed ad libitum a basal commercial grower mash diet; 3 d of feed withdrawal; feed withdrawal followed by 1 d of ad libitum access to the same commercial mash diet (AFC); and free access to the commercial mash diet with 0.05, 1, or 3% SCE for 1 d. All SCE groups gained more weight in 1 d of refeeding than the AFC group (P < 0.05). Compared with the AFC group, the SCE groups increased cell mitosis (P < 0.05). On the villus apical surface, flat epithelial cells of the feed withdrawal group developed more protuberated cells than those of the intact control group in all refeeding groups. Compared with the AFC group, the SCE groups showed more protuberated cells. In addition, in the 0.05% SCE group, cell clusters aggregated by many cells were observed on the villus apical surface. The present histological intestinal alterations in chickens refed a SCE-containing diet demonstrate that the villi and epithelial cells might be hypertrophied because of some component in the SCE, resulting in quicker BW recovery in SCE-fed birds compared with those in the AFC group.

Animal Feed↗
PubMed1985

The possible relation between mastication and parotid secretion in the rabbit.

Salivary flow has been recorded from conscious rabbits during 1 min periods whilst continuously chewing standard laboratory dry pellets or pieces of carrot and, in some animals, a mash of pellets with water. Flow was measured using contact drop recorders or a continuous flow recorder via Polythene tubes permanently inserted into one or both parotid ducts. Large variations in flow were obtained with unilateral recordings particularly during dry pellet chewing. Bilateral recordings showed that the flow was always greater on one side than on the other and that dominant secretion alternated from side to side in an apparently random manner. Rabbits chew unilaterally. Videotaped recordings of chewing movements showed that the greater secretion was always produced on the chewing side. To test the possibility that drying of the oral mucosa, or the prolonged hardness of the pellets may result in higher flow rates in animals with cannulated ducts than would normally be seen in intact animals, water was injected downstream into the mouth through a third cannula. This was inserted in an anterograde direction in the parotid duct on one side. Significant reductions in flow were recorded during dry pellet eating, but not during carrot eating. When animals were fed a soft pellet mash, salivary flow was significantly lower than with dry pellets. Recordings have been made from strain gauges attached to the ascending ramus of the mandible. Previous findings that dry pellets produce greater strain than carrots have been confirmed. It has also been shown that less strain is produced with soft pellet mash. The strain gauge data suggested that a relation exists between masticatory force and parotid salivary flow. The results are compatible with the hypothesis that intra-oral mechanoreceptors may be involved in a masticatory-salivary reflex.

Animals↗
PubMed1994

Fuel alcohol production: optimization of temperature for efficient very-high-gravity fermentation.

The time required to end ferment wheat mash decreased as the temperature was increased from 17 to 33 degrees C, but it increased as the concentration of dissolved solids was raised from 14.0 to 36.5 g/100 ml. Ethanol yield was not appreciably affected. Over the range of fermentation temperatures tested, the addition of urea accelerated the rate of fermentation, decreased the time required to complete fermentation at all dissolved-solid concentrations, and stimulated the production of slightly more ethanol than was produced by the corresponding unsupplemented control mashes. The optimum temperature for maximum ethanol production in urea-supplemented very-high-gravity wheat mash was 27 degrees C. These data are important for the industrial assessment of very-high-gravity fermentation technology.

Journal Article↗
PubMed1997

Effects of lactobacilli on yeast-catalyzed ethanol fermentations.

Normal-gravity (22 to 24 degrees Plato) wheat mashes were inoculated with five industrially important strains of lactobacilli at approximately 10(5), approximately 10(6), approximately 10(7), approximately 10(8), and approximately 10(9) CFU/ml in order to study the effects of the lactobacilli on yeast growth and ethanol productivity. Lactobacillus plantarum, Lactobacillus paracasei, Lactobacillus #3, Lactobacillus rhamnosus, and Lactobacillus fermentum were used. Controls with yeast cells but no bacterial inoculation and additional treatments with bacteria alone inoculated at approximately 10(7) CFU/ml of mash were included. Decreased ethanol yields were due to the diversion of carbohydrates for bacterial growth and the production of lactic acid. As higher numbers of the bacteria were produced (depending on the strain), 1 to 1.5% (wt/vol) lactic acid resulted in the case of homofermentative organisms. L. fermentum, a heterofermentative organism, produced only 0.5% (wt/vol) lactic acid. When L. plantarum, L. rhamnosus, and L. fermentum were inoculated at approximately 10(6) CFU/ml, an approximately 2% decrease in the final ethanol concentration was observed. Smaller initial numbers (only 10(5) CFU/ml) of L. paracasei or Lactobacillus #3 were sufficient to cause more than 2% decreases in the final ethanol concentrations measured compared to the control. Such effects after an inoculation of only 10(5) CFU/ml may have been due to the higher tolerance to ethanol of the latter two bacteria, to the more rapid adaptation (shorter lag phase) of these two industrial organisms to fermentation conditions, and/or to their more rapid growth and metabolism. When up to 10(9) CFU of bacteria/ml was present in mash, approximately 3.8 to 7.6% reductions in ethanol concentration occurred depending on the strain. Production of lactic acid and a suspected competition with yeast cells for essential growth factors in the fermenting medium were the major reasons for reductions in yeast growth and final ethanol yield when lactic acid bacteria were present.

Ethanol↗
PubMed1975

Stability of aflatoxin B-1 and ochratoxin A in brewing.

The stability of aflatoxin B-1 and ochratoxin A in brewing was investigated by adding the purified toxins to the raw materials at 1 and 10 mug/g levels during mashing in a conventional micro-brewing process. The results indicate that both toxins are stable to heat and are insensitive to cooker mash treatment. Both mycotoxins were partially removed in the mashing and brewing processes. About 14 to 18% and 27 to 28% of the added toxins were found in the final beers brewed from starting materials containing 1 and 10 mug, respectively, of either toxin per g. The possible route of transmission of mycotoxins into beer is discussed.

Aflatoxins↗
PubMed2003

Effect of fungi fermentation on organoleptic properties, energy content and in-vitro multienzyme digestibility of cassava products (flour & gari).

The present study sought to investigate the effect of fungi fermentation on the energy content, sensory quality and the digestibility (in vitro) of cassava products (flour and gari). The fungi fermented cassava products (gari and flour) were produced, by fermenting cassava mash with pure strains of some common saprophytes, namely, Aspergillus flavus, Aspergillus niger, Rhizopus oryzae and Saccharomyces spp (Baker's yeast and palm wine yeast) for 72 hrs before processing into cassava flour and gari, the forms in which cassava is popularly consumed in Nigeria. Parameters determined include energy (Bomb calorimetry), digestibility (in vitro) and sensory quality by trained taste panel. The results of the study indicated that fungi fermentation of the cassava mash significantly (P < 0.05) increased the acceptability of the colour, texture, aroma and taste of the "gari", with that of Rhizopus oryzae fermentation having the highest general acceptability. Furthermore, the results also indicated that fungi fermentation of cassava mash significantly increased (P < 0.05) the in vitro multienzyme protein digestibility of the cassava products. In view of this, fungi fermentation could be used to improve the sensory quality and protein digestibility of cassava products without any significant (P > 0.05) effect on the energy-giving role of cassava products.

Aspergillus flavus↗
PubMed2006

Effects of fat on gastric emptying of and the glycemic, insulin, and incretin responses to a carbohydrate meal in type 2 diabetes.

CONTEXT: Gastric emptying (GE) is a major determinant of postprandial glycemia. Because the presence of fat in the small intestine inhibits GE, ingestion of fat may attenuate the glycemic response to carbohydrate. OBJECTIVE: The objective of this study was to evaluate the effect of patterns of fat consumption on GE and glucose, insulin, glucagon-like peptide-1 (GLP-1), and glucose-dependent insulinotropic polypeptide (GIP) concentrations after a carbohydrate meal in type 2 diabetes. DESIGN: This was a randomized, cross-over study in which GE of a radioisotopically labeled potato meal was measured on 3 d. SETTING: The study was performed at the Royal Adelaide Hospital. PATIENTS: Six males with type 2 diabetes were studied. INTERVENTION: Subjects ingested 1) 30 ml water 30 min before the mashed potato (water), 2) 30 ml olive oil 30 min before the mashed potato (oil), or 3) 30 ml water 30 min before the mashed potato meal that contained 30 ml olive oil (water and oil). MAIN OUTCOME MEASURES: GE, blood glucose, plasma insulin, GLP-1, and GIP concentrations were the main outcome measures. RESULTS: GE was much slower with oil compared with both water (P < 0.0001) and water and oil (P < 0.05) and was slower after water and oil compared with water (P < 0.01). The postprandial rise in blood glucose was markedly delayed (P = 0.03), and peak glucose occurred later (P = 0.04) with oil compared with the two other meals. The rises in insulin and GIP were attenuated (P < 0.0001), whereas the GLP-1 response was greater (P = 0.0001), after oil. CONCLUSIONS: Ingestion of fat before a carbohydrate meal markedly slows GE and attenuates the postprandial rises in glucose, insulin, and GIP, but stimulates GLP-1, in type 2 diabetes.

Aged↗
PubMed2005

Monitoring the lactic acid bacterial diversity during shochu fermentation by PCR-denaturing gradient gel electrophoresis.

The presence of lactic acid bacteria (LAB) during shochu fermentation was monitored by PCR-denaturing gradient gel electrophoresis (DGGE) and by bacteriological culturing. No LAB were detected from fermented mashes by PCR-DGGE using a universal bacterial PCR primer set. However, PCR-DGGE using a new primer specific for the 16S rDNA of Lactococcus, Streptococcus, Tetragenococcus, Enterococcus, and Vagococcus and two primers specific for the 16S rDNA of Lactobacillus, Pediococcus, Leuconostoc, and Weissella revealed that Enterococcus faecium, Lactobacillus casei, Lactobacillus fermentum, Lactobacillus nagelii, Lactobacillus plantarum, Lactococcus lactis, Leuconostoc citreum, Leuconostoc mesenteroides, and Weissella cibaria inhabited in shochu mashes. It was also found that the LAB community composition during shochu fermentation changed after the main ingredient and water were added during the fermentation process. Therefore, we confirmed that PCR-DGGE using all three primers specific for groups of LAB together was well suited to the study of the LAB diversity in shochu mashes. The results of DGGE profiles were similar to the results of bacteriological culturing. In conclusion, LAB are present during shochu fermentation but not dominant.

Bacteria, Aerobic↗
PubMed2003

Analysis of the pyruvate permease gene (JEN1) in glucose derepression yeast (Saccharomyces cerevisiae) Isolated from a 2-deoxyglucose-tolerant mutant, and its application to sake making.

We isolated mutants of S. cerevisiae in which expression of the JEN1 gene encoding a pyruvate transporter was insensitive to glucose repression. The isolated mutant GDR19 expressed JEN1 and absorbed pyruvate in the presence of glucose. In a DNA microarray analysis, GDR19 highly expressed many more genes, including JEN1, in the presence of glucose compared with the parental strain B29. Some of these genes are under the control of the transcription factor Mig1p and are normally repressed in the presence of glucose. The concentrations of organic acids in sake made with GDR19 were different from those in sake made with B29. Changes in the pyruvate concentration in the sake mash made with GDR19 were not very different from those in sake mash made with B29, and both GDR19 and B29 expressed JEN1 during fermentation. When the ethanol concentration was over 2%, JEN1 expression in B29 was similar in the presence and absence of glucose. The expression of JEN1 in sake mash in spite of the presence of glucose appeared to be caused by the coexistence of ethanol.

Deoxyglucose↗
PubMed2004

Enteric dopaminergic neurons: definition, developmental lineage, and effects of extrinsic denervation.

The existence of enteric dopaminergic neurons has been suspected; however, the innervation of the gut by sympathetic nerves, in which dopamine (DA) is the norepinephrine precursor, complicates analyses of enteric DA. We now report that transcripts encoding tyrosine hydroxylase (TH) and the DA transporter (DAT) are present in the murine bowel (small intestine > stomach or colon; proximal colon > distal colon). Because sympathetic neurons are extrinsic, transcripts encoding TH and DAT in the bowel are probably derived from intrinsic neurons. TH protein was demonstrated immunocytochemically in neuronal perikarya (submucosal >> myenteric plexus; small intestine > stomach or colon). TH, DA, and DAT immunoreactivities were coincident in subsets of neurons (submucosal > myenteric) in guinea pig and mouse intestines in situ and in cultured guinea pig enteric ganglia. Surgical ablation of sympathetic nerves by extrinsic denervation of loops of the bowel did not affect DAT immunoreactivity but actually increased numbers of TH-immunoreactive neurons, expression of mRNA encoding TH and DAT, and enteric DOPAC (the specific dopamine metabolite). The fetal gut contains transiently catecholaminergic (TC) cells. TC cells are the proliferating crest-derived precursors of mature neurons that are not catecholaminergic and, thus, disappear after embryonic day (E) 14 (mouse) or E15 (rat). TC cells appear early in ontogeny, and their development/survival is dependent on mash-1 gene expression. In contrast, the intrinsic TH-expressing neurons of the murine bowel appear late (perinatally) and are mash-1 independent. We conclude that the enteric nervous system contains intrinsic dopaminergic neurons that arise from a mash-1-independent lineage of noncatecholaminergic precursors.

3,4-Dihydroxyphenylacetic Acid↗
PubMed2005

Individual and combined effects of Fusarium moniliforme culture material, containing known levels of fumonisin B1, and Salmonella gallinarum infection on liver of Japanese quail.

Three hundred day-old Japanese quail (Coturnix coturnix japonica) were divided into two groups with 150 quail in each group. One group was maintained on quail mash alone, while Fusarium moniliforme culture material was added to quail mash in the second group from day 5 of age and was supplied at a rate of 150 ppm fumonisin B1 (FB1)/kg mash. At day 21, each group was further subdivided into two groups, yielding four groups with 75 birds apiece, which served as the control (group CX), the Salmonella Gallinarum alone group (group CS), the FB1 alone group (group FX), and the group fed FB1 and infected with Salmonella Gallinarum (group FS). An oral challenge with Salmonella Gallinarum organisms (2 x 10(4) colony-forming units/ml) was given to groups CS and FS at 21 days of age. Three quail each were necropsied on day 21 (0 day interval) from groups CX and FX only. At subsequent intervals (i.e., 1, 2, 3, 5, 7, 10, 14, and 21 days postinfection [DPI]), three quail were euthanatized from all four groups (CX, CS, FX, and FS). The gross and microscopic lesions were recorded in both mortality and euthanatized birds at the above intervals. The ultrastructural studies were done at 5 DPI. Mild to moderate hepatomegaly and pale discoloration of liver were observed in group FX, while congestion, hemorrhages, necrosis, and mild to severe hepatomegaly were the predominant gross lesions in both infected groups (CS and FS). The gross lesions in quail inoculated with Salmonella Gallinarum alone (group CS) generally developed slowly, appeared more widely scattered, and involved comparatively less surface area in contrast to the rapidly progressive and frequently confluent lesions in the combination group (FS), especially in the first 5 days of infection. Mild to marked hepatocellular swelling, multifocal hepatic necrosis, and hepatocellular and bile duct hyperplasia were the characteristic microscopic changes in the FX group. Microscopic lesions in quail of group CS comprised congestion, vacuolar changes, and focal necrosis in early stages, followed by granulomatous lesions at later intervals. Similar but more severe lesions were observed in the combination group (FS). Based on transmission electron microscopy, the maximum effect of FB1 toxicity was observed on mitochondria and endoplasmic reticulum. In general, the mitochondriae showed diverse form and structure, some of which appeared to lose their intact outer membrane, and the mitochondrial cristae were disoriented. The deformity in the cisternae structure of rough endoplasmic reticulum, with their rearrangement into round or tubular forms either bearing granular surface or leading to accumulation of smooth endoplasmic reticulum, was evident only in groups FX and FS. We conclude that the continuous presence of fumonisins in the diets of young quail might increase their susceptibility to or the severity of Salmonella Gallinarum infection.

Animals↗
PubMed1987

An animal model of excessive eating: schedule-induced hyperphagia in food-satiated rats.

Nineteen rats were maintained throughout the experiment on ad libitum wet mash and water and were trained to press a lever on fixed-interval or fixed-ratio schedules of reinforcement with electrical brain stimulation. Fourteen rats ate at least 150% more mash during intermittent reinforcement sessions than during baseline, massed reinforcement control, and/or extinction sessions. In a 3-hr session, 11 of those 14 consumed more than 22 g of wet mash (13 g dry weight), the equivalent of nearly half an animal's daily food intake. In subsequent control sessions, the electrodes did not support stimulus-bound eating despite attempts to make stimulation parameters optimal. These results indicate that the eating was schedule induced or adjunctive, and suggest that the procedure may provide an animal model of excessive nonregulatory eating that contributes to obesity in humans.

Animals↗
PubMed1982

The effect of crumbled and pelleted feed on the incidence of sudden death syndrome among male chicken broilers.

An experiment involving 7200 male broiler chickens was conducted to estimate the effect of feeding crumble-pellet diets on the incidence of Sudden Death Syndrome (SDS). Mortality due to SDS was significantly higher for birds fed a crumble-pellet regimen in its usual form or in a ground form compared with birds fed all-mash diets. Birds fed the crumble-pellet dietary regimen grew more rapidly than those on either the ground crumble-pellet regimen or on all-mash. There was no significant (P = .05) difference in the growth rate of the birds fed the ground crumble-pellet regimen compared with those fed all-mash. It was concluded that the higher incidence of SDS was due to some factor(s) in the pelleting process itself rather the rapid growth resulting from the higher density of pelleted feeds.

Animal Feed↗
PubMed1983

Anticoccidial drugs and duckling performance to four weeks of age.

Frequently, publications pertaining to waterfowl state that medicated feeds should not be fed to ducklings and goslings. In some localities, producers and hobbyists who raise a small number of ducklings and goslings can purchase only medicated chick, turkey, or gamebird starter and grower feeds. Because of the lack of documented information on this subject and the numerous requests for advice on this matter, anticoccidial drugs, zoalene, sulfaquinoxaline, and amprolium, were mixed in mash feed and fed to Khaki Campbell male ducklings to 4 weeks of age. No significant differences in mean body weight, mortality, and anatomical development were observed among the treatments. Medicated commercial crumble turkey and chick starter feeds produced significantly better feed conversion than the mash medicated or nonmedicated feeds. These differences can be attributed to greater feed spillage with the mash feed. Some ducklings in all treatments showed unsteadiness of gait and shaky legs. These conditions disappeared when the ducklings were moved from the battery brooder to an outside pen. Zoalene, sulfaquinoxaline, and amprolium used at the recommended levels for chickens and turkeys did not cause any leg or anatomical problems in ducklings.

Amprolium↗
PubMed1983

Salmonella and other Enterobacteriaceae found in commercial poultry feed.

Poultry feed (mash and pelleted) and meat and bone meal samples were collected from commercial mills. All samples were analyzed for Enterobacteriaceae count (ENT) and Salmonella. The genus and species of the various Enterobacteriaceae present were also determined. The average ENT for mash, pelleted, and meal samples was log 4.1, .8, and 1.8/g, respectively. Enterobacteriaceae were present in 100, 60, and 92% and Salmonella in 58, 0, and 92% of the mash, pelleted, and meal samples, respectively. Overall, the Enterobacteriaceae most frequently isolated from all samples were Enterobacter agglomerans, Enterobacter cloacae, and Klebsiella pneumoniae. Although no Salmonella were found in the pelleted samples, the presence of other Enterobacteriaceae suggests that commercial pelleting may not totally destroy Salmonella since their heat resistance is similar to the other organisms found.

Animal Feed↗