Intradermal testing after severe reactions to anaesthetic drugs.
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A purified concentrated BHV1 antigen that had been tested in field trials was also tested under isolated conditions in BHV1 positive and negative cattle. The antigen proved to act specifically even after storage for 2 years at +4 degrees C or for 6 months at 37 degrees C. The best results were obtained when the test was read 48-72 hours after the injection, which is in agreement with the field trials. The increase in skin thickness decreased unless boosted by infection or vaccination gradually. The test is unsuitable to control a vaccination programme. There was no correlation between the increase in thickness and humoral antibody titers. Repeated application of the intradermal injection of the antigen did not result in seroconversion in seronegative cattle. The biological limits of the test evaluation are discussed.
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Pollen of Artemisia annua is considered to be one of the most important allergens in autumnal hay fever in China, just as ragweed is in North America. In order to clarify the allergenicity of non-pollen containing components of the plant, Artemisia annua leaves and stems were collected and extracted before pollination time. The extracts of these pollen-free plant components were studied for allergenic activities using skin prick tests, intradermal tests, intranasal challenge and bronchial provocation tests. In 52 subjects sensitive to Artemisia pollen, 92.3% gave positive responses in skin prick tests, 100% gave positive responses in intradermal tests, 66.7% gave positive responses in intranasal challenge and 59.3% gave positive responses in bronchial provocation tests. Negative results to skin prick tests, intradermal tests and bronchial provocation tests were revealed in 30 seasonal asthmatics who gave negative responses to the pollen skin tests. Strict placebo control showed all negative tests in non-atopic adult human volunteers. We concluded that pollen-free plant extracts did have in vivo allergenic activities. Analysis of the plant allergenic components in vitro will be the subject of further studies.
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