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Regional strengths of bonding agents to cervical sclerotic root dentin.

The regional bond strengths of three current-generation bonding systems (All Bond 2, Scotchbond Multi-Purpose, and Clearfil Liner Bond 2) were measured in natural wedge-shaped defects in the cervical area of extracted human teeth. A microtensile testing method was used to compare the strengths of resin bonds made to occlusal margins with those made to gingival margins. Controls consisted of normal teeth which had artificial wedge-shaped defects, of the same depth and dimension, created with a high-speed bur. The results indicated that there were no regional differences in bond strength, although bonds made to natural lesions were from 20 to 45% lower than those made to normal dentin in artificially created wedge-shaped defects, depending on the bonding agent. Scanning electron microscopy revealed that Clearfil Liner Bond 2 created the thinnest hybrid layers, which were difficult to measure in the natural lesions. The natural lesions contained sclerotic dentin, whereas the artificial lesions were composed of normal dentin. Although the bond strengths to sclerotic dentin were lower than those to normal dentin, the absolute values (ca. 16 to 17 MPa) were high relative to previous-generation bonding agents.

Acid Etching, Dental↗

Effect of perfusion with water on demineralization of human dentin in vitro.

Dentinal fluid rarely features in caries studies of dentin. The mutual effects of in vitro perfusion and dentin demineralization were investigated. The correlation between the remaining dentin thickness and demineralization was also analyzed. Buccal cervical dentin windows in human tooth segments were demineralized either with or without perfusion with water at 3.14 kPa. Transverse microradiography revealed that dentin perfusion reduced the amount of mineral loss from the lesions by 22 vol%; the reduction in lesion depth was 8%. Perfusion rate, which was measured throughout the demineralization process by means of a fluid transport model, did not change significantly. Lesions formed closer to the pulp exhibited increased mineral loss and lesion depth. In conclusion, dentinal fluid flow offers some protection against demineralization. For a better approximation of clinical reality, therefore, in vitro studies on dentinal caries should consider the effect of dentinal fluid flow.

Analysis of Variance↗

Incompatibility of oxalate desensitizers with acidic, fluoride-containing total-etch adhesives.

The use of oxalate desensitizers on acid-etched dentin prior to adhesive application can result in subsurface tubular occlusion by calcium oxalate crystals. However, the solubility of calcium oxalate increases in acidic solution. We hypothesized that total-etch adhesives can, depending upon their pH, interact with oxalate-desensitizer-treated dentin in an adverse manner. Acid-etched human dentin treated with 2 oxalate desensitizers (BisBlock and Super Seal) was bonded with 4 simplified total-etch adhesives: One-Step (OS), Single Bond (SB), OptiBond Solo Plus (OB), and Prime&Bond NT (PB). Composite-dentin beams were examined by SEM and TEM, both of which revealed numerous spherical globules on OB- and PB-bonded, desensitizer-treated dentin, but not in OS or SB samples. Bond strengths produced by OB and PB were significantly lower in oxalate-treated specimens than those produced by OS or SB. These surface globules may have interfered with hybridization of demineralized dentin with OB and PB resins and caused compromised bond strengths.

Analysis of Variance↗

Reduced antigenicity of type I collagen and proteoglycans in sclerotic dentin.

Antigenic alterations to the dentin organic matrix may be detected by an immunohistochemical approach. We hypothesized that alterations in the antigenicity of type I collagen and proteoglycans occur in sclerotic dentin under caries lesions. Transverse sections were prepared from carious teeth in the sclerotic zone and normal hard dentin. A double-immunolabeling technique was performed on these sections, with anti-type I collagen and anti-chondroitin 4/6 sulfate monoclonal primary antibodies. We used gold-conjugated secondary antibodies to visualize the distribution of intact collagen fibrils and proteoglycans by high-resolution SEM. For sclerotic dentin, labeling densities were 19.57 +/- 3.01/microm2 for collagen and 9.84 +/- 2.62/microm2 for proteoglycans. For normal hard dentin, values were 35.20 +/- 2.73/microm2 and 17.03 +/- 1.98/microm2, respectively. Distribution of intact collagen fibrils and proteoglycans in sclerotic dentin was significantly lower than in normal hard dentin. Reductions in antigenicity from the organic matrix of sclerotic dentin under caries lesions raise concern about the potential of intrafibrillar remineralization.

Collagen Type I↗

Invasion of dentinal tubules by oral bacteria.

Bacterial invasion of dentinal tubules commonly occurs when dentin is exposed following a breach in the integrity of the overlying enamel or cementum. Bacterial products diffuse through the dentinal tubule toward the pulp and evoke inflammatory changes in the pulpo-dentin complex. These may eliminate the bacterial insult and block the route of infection. Unchecked, invasion results in pulpitis and pulp necrosis, infection of the root canal system, and periapical disease. While several hundred bacterial species are known to inhabit the oral cavity, a relatively small and select group of bacteria is involved in the invasion of dentinal tubules and subsequent infection of the root canal space. Gram-positive organisms dominate the tubule microflora in both carious and non-carious dentin. The relatively high numbers of obligate anaerobes present-such as Eubacterium spp., Propionibacterium spp., Bifidobacterium spp., Peptostreptococcus micros, and Veillonella spp.-suggest that the environment favors growth of these bacteria. Gram-negative obligate anaerobic rods, e.g., Porphyromonas spp., are less frequently recovered. Streptococci are among the most commonly identified bacteria that invade dentin. Recent evidence suggests that streptococci may recognize components present within dentinal tubules, such as collagen type I, which stimulate bacterial adhesion and intra-tubular growth. Specific interactions of other oral bacteria with invading streptococci may then facilitate the invasion of dentin by select bacterial groupings. An understanding the mechanisms involved in dentinal tubule invasion by bacteria should allow for the development of new control strategies, such as inhibitory compounds incorporated into oral health care products or dental materials, which would assist in the practice of endodontics.

Animals↗

Sensitivity answers sought in amalgam alloy microleakage study.

Sensitivity often occurs shortly after an amalgam restoration is placed. This phenomenon has been related in part to microleakage at the amalgam/tooth interface. When microleakage characteristics of a large number of commercially available dental amalgams were examined, results showed wide variation in the amount of microleakage.

Analysis of Variance↗

Managing dentin hypersensitivity.

BACKGROUND: The objective of this review is to inform practitioners about dentin hypersensitivity (DH) and its management. This clinical information is described in the context of the underlying biology. TYPES OF STUDIES REVIEWED: The authors used MEDLINE to find relevant English-language literature published in the period 1999 to 2005. They used combinations of the search terms "dentin*," "tooth," "teeth," "hypersensit*," "desensiti*" and "desensitiz*." They read abstracts and then full articles to identify studies describing etiology, prevalence, clinical features, controlled clinical trials of treatments and relevant laboratory research on mechanisms of action. RESULTS: The prevalence of DH varies widely, depending on the mode of investigation. Potassium-containing toothpastes are the most widely used at-home treatments. Most in-office treatments employ some form of "barrier," either a topical solution or gel or an adhesive restorative material. The reported efficacy of these treatments varies, with some having no better efficacy than the control treatments. Possible reasons for this variability are discussed. A flowchart summarizes the various treatment strategies. CLINICAL IMPLICATIONS: DH is diagnosed after elimination of other possible causes of the pain. Desensitizing treatment should be delivered systematically, beginning with prevention and at-home treatments. The latter may be supplemented with in-office modalities.

Adhesives↗

Short-term assessment of the Nd:YAG laser with and without sodium fluoride varnish in the treatment of dentin hypersensitivity--a clinical and scanning electron microscopy study.

BACKGROUND: The aim of the present study was to evaluate clinically and under scanning electron microscopy (SEM) the efficacy of Nd:YAG laser irradiation alone and in combination with 5% sodium fluoride varnish in the management of dentin hypersensitivity. METHODS: The study was conducted on 40 patients divided into four groups who had at least one tooth of Grade III mobility with clinically elicitable dentin hypersensitivity. Following the pretreatment assessment of hypersensitivity using the visual analog scale (VAS) and cold air blast test, the selected tooth in all the groups received 1% citric acid treatment for 1 minute. Group 1 patients received no further treatment; group 2, 3, and 4 patients received additional treatment with 5% sodium fluoride varnish, Nd:YAG laser for 2 minutes, and a combination of 5% sodium fluoride varnish and Nd:YAG laser, respectively. Two hours following treatment, hypersensitivity was again assessed, and the teeth were extracted, sectioned, and scanned using scanning electron microscopy (SEM). RESULTS: The mean VAS score in group 1 showed a 27% increase from baseline, but groups 2, 3, and 4 showed a decrease of 33%, 44%, and 62%, respectively. The mean cold air blast score showed a 22% increase in group 1, but values decreased by 43%, 50%, and 83% in groups 2, 3, and 4, respectively. The number of patent tubules also progressively decreased from group 1 through group 4. CONCLUSIONS: The combination of Nd:YAG laser and 5% sodium fluoride varnish seems to show an impressive efficacy, when compared to either treatment alone, in treating dentin hypersensitivity. The SEM findings seem to relate to the clinical findings in that reduction in number/patency of tubules was associated with improvement in treatment efficacy.

Adult↗

Effects of plaque control on the patency of dentinal tubules: an in vivo study in beagle dogs.

BACKGROUND: The aim of this in vivo study was to evaluate the effects of plaque control on the patency of dentinal tubules using vital teeth of beagle dogs. METHODS: Class V cavities were prepared on the cervical areas of the mandibular and maxillary molars in each dog with a diamond point. To simulate the state of dentinal hypersensitivity, the teeth were etched with 50% citric acid for 2 minutes to obtain patent dentinal tubules. Plaque control was achieved by brushing the left-side teeth every day, whereas no plaque control was performed for the right-side teeth. A dentin biopsy was performed after 1, 2, and 3 weeks using the cylindrical diamond point to obtain dentin specimens. RESULTS: In the plaque control group, some of the dentinal tubules were occluded with precipitate (Ca/P=1.49), and the diameter of the dentinal tubules decreased from 2.42+/-0.33 microm (mean+/-SD) to 1.11+/-0.51 microm after 7 days, although most of the dentinal tubules remained open. In contrast, no precipitate was observed in the dentinal tubules of the non-plaque control group. Also, the diameter of the dentinal tubules increased from 2.42+/-0.33 to 2.9+/-0.49 microm, due to the demineralization of the peritubular and intertubular dentin. CONCLUSION: Plaque control plays a key role in reducing the patency of dentinal tubules and, therefore, might promote the natural repair of dentinal hypersensitivity.

Analysis of Variance↗

Durability of FTLA treatment as a medicament for dentin hypersensitivity--abrasion resistance and profiles of fluoride release.

The purpose of this study was to evaluate the durability of tubules occluded with FTLA treatment by toothbrush abrasion test on the applied surface and by measuring fluoride release from the FTLA components. Dentin specimens with simulated hypersensitive surfaces were treated with APF containing tannic acid. After which, the specimens received lanthanum-chloride-with-powdered-fluoroapatite-glass-ceramics treatment. The specimens were subjected to toothbrush abrasion test up to 6,000 strokes. SEM observation revealed that dentinal tubules of the FTLA treated specimens were completely occluded with fine deposits even after toothbrush abrasion of 6,000 strokes. EPMA analysis revealed that fluoride, lanthanum, and aluminum were the main FTLA components on the dentin surface after 6,000-stroke abrasion. To measure fluoride release from the FTLA components, a slurry was enclosed in a cellulose tube and suspended in deionized water at 37 degrees C. After fluoride was dialyzed against deionized water, a high concentration of fluoride was found to be released from FTLA the components, indicating FTLA treatment's prominent durability. These results suggested that FTLA treatment has a superior resistance against toothbrush abrasion and a high fluoride-releasing performance. These characteristics lend much weight to showing that the FTLA method is an effective and durable medicament for dentin hypersensitivity.

Acidulated Phosphate Fluoride↗

Comparison of the occluding ability of dentinal tubules with different morphology between calcium phosphate precipitation method and potassium oxalate treatment.

The aim of this study was to evaluate the occluding ability of calcium phosphate precipitation (CPP) method and potassium oxalate treatment when each method was applied to dentin disks with different surface morphology. Occluding ability was observed by scanning electron microscopy. Irrespective of the diameter of the dentinal tubules, the CPP method showed a consistent occluding ability for dentinal tubules at the dentin surface, and that the depths of the precipitate formed in the dentinal tubules by CPP method were not significantly different. In contrast, the occluding ability of potassium oxalate treatment was reduced with increasing diameter of the dentinal tubules. However, the reduction of the occluding ability of potassium oxalate treatment was more markedly affected by the demineralization of dentin surface. Since the CPP method showed a consistent occluding ability irrespective of the diameter of the dentinal tubules, it is suggested that the CPP method would be a useful means for treating dentin hypersensitivity.

Adult↗

In vitro study of DP-bioglass paste for treatment of dentin hypersensitivity.

Sealing of exposed dentinal tubules is generally considered the most effective strategy to treat dentin hypersensitivity. On this account, we fabricated a DP-bioglass paste that created a homogeneous blockage on open dentinal tubules and formed a deep precipitate within dentinal tubules. DP-bioglass paste was prepared by mixing 20% to 60% phosphoric acid and DP-bioglass to treat dentin surfaces. CO2 laser irradiation was used to melt the DP-bioglass paste. The results demonstrated that 30% phosphoric acid was the optimum concentration to produce homogeneous occlusion on exposed dentinal tubules and 60 microm of sealing depth. CO2 laser irradiation could melt the DP-bioglass paste and create about 10 microm of sealing depth. Moreover, temperature rise during CO2 laser irradiation was only 4.86 +/- 0.47 degrees C. The results presented in this work suggested that DP-bioglass paste could produce considerable sealing depth in dentinal tubules with the potential of prolonging the therapeutic effect efficaciously.

Adolescent↗

Hardness and Young's modulus of transparent dentin associated with aging and carious disease.

This study investigated the changes in hardness and Young's modulus of the transparent layer of dentin associated with aging and the carious process. Eighteen extracted human molars with or without coronal caries were used in this study. The normal teeth were divided into two groups by age, and the carious teeth were divided into two groups of active or arrested caries. After polishing the specimens parallel to the long axis of the tooth, both hardness and Young's modulus were measured using a nanoindentation tester. The hardness and Young's modulus of the transparent layer in aged dentin were higher than the other portions of aged dentin. The transparent layer under carious lesions had a significantly lower hardness than the underlying normal dentin, whereas its Young's modulus was not significantly reduced. The hardness and Young's modulus of the transparent layer in active carious lesions were lower than those in arrested carious lesions.

Aging↗

Thickness and morphology of resin-infiltrated dentin layer in young, old, and sclerotic dentin.

The purpose of the present study was to evaluate the morphology of the resin tags and the resin-infiltrated dentin layer (RIDL) of several bonding systems in superficial vs deep young, old, and sclerotic human dentin. Dentin was obtained after the removal of occlusal enamel from extracted molars. Phosphoric acid gels (35-37%) were used to etch dentin before the application of bonding systems (OptiBond FL; Prime & Bond 2.0; Scotchbond Multi-Purpose Plus and Scotchbond 1; One Step). Each bonded specimen was then sectioned into two halves. One half was polished using a standard procedure to evaluate RIDL thickness and morphology by SEM. The other half was demineralized and deproteinized to evaluate the presence and the morphology of resin tags. RIDL was thinner in superficial dentin than in deeper dentin for all the materials tested regardless of the type of dentin. Sclerotic and old dentin showed thinner RIDL, with short resin tags, and fewer lateral branches than normal dentin.

Acid Etching, Dental↗

Clinical evaluation of a one-bottle bonding system for desensitizing exposed roots.

PURPOSE: To evaluate the effectiveness of a one-bottle adhesive system as a desensitizing treatment, with or without a prior phosphoric acid-etch step. MATERIALS AND METHODS: Two different clinical procedures using a one-bottle adhesive bonding system were tested for its efficacy on desensitizing exposed roots. Thirty subjects with a history of dentin hypersensitivity were selected and 60 exposed sensitive teeth were divided into two groups (n = 30). In Group 1 samples, Scotchbond 1 bonding system (Single Bond in USA) was applied without a prior acid-etching, while in those of Group 2, the exposed sensitive roots were acid-etched with 35% phosphoric acid for 15 seconds, prior to application of bonding agents. The sensitivity was evaluated at 0, 1, 2, 4 weeks and 3 months using cold air blast stimuli, and recorded using a visual-analogue scale. The scores were analyzed as non-parametric data by means of the Mann-Whitney and Sign tests. Five samples from each group were randomly selected in order to make scanning electron microscope observations of the sensitive dentin surfaces at baseline and at each recall using the replica technique. RESULTS: At baseline, after both desensitizing procedures all the patients reported distinct reductions in dentin sensitivity. At 1- and 2-week recalls, 20 teeth of Group 1 and 17 teeth in Group 2 exhibited a return of sensitivity, and in five teeth in each group, the sensitivity score was between 5-8. During the first recall (1-2 weeks), the sensitive teeth were retreated and showed an immediate reduction of sensitivity. At the 1-month recall, eight teeth in Group 1 and four teeth in Group 2 showed a return of some sensitivity, and in six teeth in Group 1 and in three teeth in Group 2, old amalgam restorations were replaced and this procedure almost completely resolved the sensitivity. At the 3-month recall, only five teeth from each group showed residual mild sensitivity (score 1-2). For both Groups 1 and 2 there was a statistically significant (P < 0.0005) reduction in sensitivity levels between the baseline pain scores and immediately after treatment, and between the first recall pre-treatment pain scores and the last recall pain scores. Overall, the two methods of treatment demonstrated a general decrease in sensitivity in both groups over the 3-month study period, adequately solving the clinical and patients' perception of the discomfort of sensitivity. The SEM observations of the replicas showed a progressive loss of the adhesive resin from the treated surfaces. This fact was more evident in the non-etched samples (Group 2) than in those in Group 1. The results of this study showed that exposed root dentin sealed with Scotchbond 1, with or without prior acid-etching, did not show spontaneous sensitivity after 3 months.

Acid Etching, Dental↗

Clinical effectiveness of a Class V flowable composite resin system.

This clinical study evaluated a Class V flowable composite resin restoration system and its ability to reduce dentin sensitivity. Twenty-one patients required Class V restorations because of erosion, abrasion, or decay at the cervical area. Each patient exhibited moderate or severe sensitivity and required replacement of tooth structure. The degree of sensitivity was determined by either tactile scraping of the tip of an explorer over the surface of the tooth or by an air blast from a syringe for 5 seconds. A total of 52 restorations were performed. According to phone conversations and questionnaires, 1 patient experienced continued sensitivity on 1 treated tooth for a duration of 1 week, but this sensitivity was gone at 1-month, 3-month, 6-month, and 1-year recalls. None of the other patients exhibited sensitivity at any phone checks or during recall visits.

Composite Resins↗

Dental applications of amorphous calcium phosphates.

Certain commercial materials and equipment are identified in this paper to specify the experimental procedure. In no instance does such identification imply recognition or endorsement by the National Institute of Standards and Technology or the ADA Health Foundation, or that the material or equipment identified is necessarily the best available for the purpose.

Calcium Phosphates↗