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Cadmium, lead and mercury levels in feeding yeast produced in Czechoslovakia.

Ninety-six samples of the feeding yeast known as VITEX were analyzed for Cd, Pb and Hg content during 1987-1989. Cadmium content ranged from 0.30 to 5.12 mg/kg(-1), lead content from 0.21 to 3.01 mg/kg(-1) and mercury content from 0.008 to 0.187 mg/kg(-1). Our findings meet the current government standards (max. allowed Pb = 5.00, Cd = 0.50 and Hg = 0.100 mg/kg(-1)) only for lead, and with five exceptions, for mercury. With two exceptions, all cadmium levels found in the samples exceeded the limit. One raw material - the wood chips - was shown to be the main source of cadmium in the technological process. Relatively high Hg contents were measured in the wood chips (up to 0.155 mg/kg(-1)); the highest Hg level (1.105 mg/kg(-1)) however was found in a sample of KOH.

Animal Feed↗

Microbiological, chemical and toxicological characterization of contaminated sites in Czechoslovakia.

We investigated the contaminated area near the wood-preserving factory at the town Sobĕslav in South Bohemia. The factory has been working for approximately 90 years. The soils of this area are extremely contaminated with the polyaromatic hydrocarbons (PAHs) derived from the creosote oils and heavy metals (predominantly Zn, Cu, Hg). The biological activities of sample contaminated soils are very low compared with unpolluted soil. The comparison of actual and potential activity between samples is evaluated for the prediction of the efficiency of application of a bioremediation processes. The testing of frequency of occurrence of a bacterium resistant to heavy metals (Zn and Cu) is used for prediction of possible negative effects on the biodegradation of organic pollutants at the concentrations of heavy metals in soils. A concentration of 2 mmol/l zinc in medium (according Houba-Remarcle) does not negatively influence the number of bacteria. A concentration of 2 mmol/l copper practically does not influence the number of bacteria. Several bacterial strains are able to survive concentrations of 20 mmol/l zinc, 8.5 mmol/l copper and 2 mmol/l cadmium. The preliminary results show that practically all strains which are resistant to heavy metals are able to degrade fluoranthene, as a representative PAH. The real concentrations of heavy metals in soils are below the levels where heavy metals negatively influence the growth of bacteria.

Colony Count, Microbial↗

First strains of the genus Kluyvera in Czechoslovakia.

Fourteen of 21 strains isolated from stools, urine specimens and the hospital environment were identified as Kluyvera. All of these 14 strains corresponded with the literary description of the genus Kluyvera and were identical with two reference strains except that one of them failed to utilize sodium acetate within 7 days. One strain (No. 23441) produced massive growth on Jordan's tartrate, which some Kluyvera do. Important in differentiating indole- and Simmons' citrate-negative Kluyvera strains from Escherichia vulneris (two other of our 21 strains) is negative ornithin decarboxylase and negative Christensen citrate in the latter. Three strains were identified as Citrobacter, where especially indole-positive and urease-negative strains may be reminiscent of Kluyvera. An aberrant strain, No. 25115, which alone failed to grow at 42 degrees C and by some characters differed from Kluyvera, E. vulneris and Enteric Group 10, was identified as E. coli.

Acetates↗

Investigations of ticks for the presence of Borrelia in Czechoslovakia.

More than 570 ticks from different parts of the country were studied microscopically. In 45 cases (8%) Borreliae were found. The infectivity rate of ticks in different foci varied from 3-11%, in some instances nearly 20%. Only Ixodes ricinus, male and female ticks were positive in this study.

Animals↗