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Isolation and characterization of virus-specific double-stranded DNA from tissues infected by bean golden mosaic virus.

A double-stranded (ds) DNA which may be a replication intermediate was isolated from bean (Phaseolus vulgaris L. "Top Crop") leaves systemically infected with bean golden mosaic virus, a whitefly-transmitted plant virus with a genome of circular single-stranded (ss) DNA. The isolation method used phenol/chloroform extraction, hydroxyapatite column chromatography, and rate-zonal centrifugation. The dsDNA had sequences complementary to those of viral DNA. The guanine-plus-cytosine content was 35%, and the sedimentation coefficient in alkaline sucrose density gradients was similar to that of viral ssDNA. Digestion of the dsDNA by Hha I endonuclease produced fragments that corresponded exactly in number and size with those produced by complete digestion of circular viral ssDNA by Hha I, when the fragments were denatured and analyzed on polyacrylamide gels. The dsDNA molecule was a circular structure with one discontinuity in one strand; hybridization results suggest that some of a the dsDNA has a discontinuity in the viral strand and some has a discontinuity in the nonviral strand. On the basis of these structures for the dsDNA, a preliminary model for replication of viral DNA is discussed.

Journal Article↗

Effect of hypobaric conditions on ethylene evolution and growth of lettuce and wheat.

Elevated levels of ethylene occur in enclosed crop production systems and in spaceflight environments, leading to adverse plant growth and sterility. There are engineering advantages in growing plants at hypobaric (reduced atmospheric pressure) conditions in biomass production for extraterrestrial base or spaceflight environments. Objectives of this research were to characterize the influence of hypobaria on growth and ethylene evolution of lettuce (Lactuca sativa) and wheat (Triticum aestivum). Plants were grown under variable total gas pressures [from 30 to 101 kPa (ambient)]. In one study, lettuce and wheat were direct seeded, germinated and grown in the same chambers for 28 d at 50 or 101 kPa. Hypobaria increased plant growth and did not alter germination rate. During a 10-day study, 28-day-old lettuce and 40-day-old wheat seedlings were transplanted together in the same low and ambient pressure chambers; ethylene accumulated in the chambers, but the rate of production by both lettuce and wheat was reduced more than 65% under 30 kPa compared with ambient pressure (101 kPa). Low O2 concentrations [partial pressure of O2 (pO2) = 6.2 kPa] inhibited ethylene production by lettuce under both low (30 kPa) and ambient pressure, whereas ethylene production by wheat was inhibited at low pressure but not low O2 concentration. There was a negative linear correlation between increasing ethylene concentration and decreasing chlorophyll content of lettuce and wheat. Lettuce had higher production of ethylene and showed greater sensitivity to ethylene than wheat. The hypobaric effect on reduced ethylene production was greater than that of just hypoxia (low oxygen).

Atmospheric Pressure↗

Review of sorption and diffusion of lipophilic molecules in cuticular waxes and the effects of accelerators on solute mobilities.

Many agrochemicals are applied to the leaf surfaces of crop plants. Systemic chemicals have to penetrate through the cuticle, which forms an effective transport barrier. The barrier properties of cuticles are mainly due to the cuticular waxes deposited as partially crystalline aggregates on the outer surfaces of leaves. Substances increasing the mobilities of agrochemicals in cuticular waxes are called accelerators and it is shown that they act as plasticizers when absorbed by cuticular waxes. They decrease the barrier properties of the waxes and thus increase the mobilities of the agrochemicals through them. In order to analyse the efficiency of different accelerators, the sorption and mobility of both agrochemicals and accelerators within cuticular waxes was measured. Such information was used to establish correlations between the internal concentrations of accelerators and their mobility-enhancing effects on agrochemicals in the cuticle. This, in turn, allowed the determination and comparison of the intrinsic effects of different accelerators and to rationalize the effect of accelerators on the cuticular permeability of agrochemicals. Results describing the sorption (partition coefficients) and mobility (diffusion coefficients) of lipophilic organic molecules in reconstituted cuticular waxes from different plant species, and the effect of two different classes of accelerators (alcohol ethoxylates and n-alkyl esters), on the mobility of organic molecules are presented and discussed.

Agrochemicals↗

Root system architecture determines fitness in an Arabidopsis mutant in competition for immobile phosphate ions but not for nitrate ions.

Plant root systems often have complex branching patterns. Models indicate that a complex architecture is only required for the acquisition of immobile resources, such as phosphate; mobile ions, notably nitrate, can be effectively taken up by very restricted root systems. We have tested this prediction using the axr4 mutation of Arabidopsis thaliana, the principal phenotypic effect of which is to reduce the number of lateral roots. Arabidopsis thaliana is not a host for mycorrhizal fungi and so acquires all its nutrients through the root system. In both a pot experiment and a field experiment conducted under natural conditions for A. thaliana, we found that only phosphate, and not nitrate, affected the fitness of the mutant relative to the isogenic wild-type line, Columbia. These results confirm model predictions and have implications both for the evolution of complex root systems and for the design of efficient root systems for crops.

Arabidopsis↗

Simulated effects of crop rotations and residue management on wind erosion in Wuchuan, west-central Inner Mongolia, China.

For decades, wind erosion has triggered dust and sand storms, buffeting Beijing and areas of northwestern China to the point of being hazardous to human health while rapidly eroding crop and livestock productivity. The EPIC (Environmental Policy Integrated Climate) field-scale simulation model was used to assess long-term effects of improved crop rotations and crop residue management practices on wind erosion in Wuchuan County in Inner Mongolia. Simulation results indicate that preserving crop stalks until land is prepared by zone tillage for the next year's crop in lieu of using them as a source of heating fuel or livestock fodder significantly reduces wind erosion by 60%. At the same time, grain and potato (Solanum tuberosum L.) yields were maintained or improved. Significant reductions in erosion, 35 to 46%, also resulted from delaying stalk removal until late January through late April. Yearly wind erosion was concentrated in April and May, the windiest months. Additionally, the use of alternative crop rotations resulted in differences in wind erosion, largely due to a difference in residue stature and quality and differences in biomass produced. As a result, altering current crop rotation systems by expanding corn (Zea mays L.), wheat (Triticum aestivum L.), and millet [Sorghum bicolor (L.) Moench] and reducing potato and pea (Pisum sativum L.) production significantly reduced simulated wind erosion, thus diminishing the severity of dust and sand storms in northwestern China. Saving and protecting topsoil over time will sustain land productivity and have long-term implications for improving conditions of rural poverty in the region.

Agriculture↗

Nitrate losses in subsurface drainage from a corn-soybean rotation as affected by time of nitrogen application and use of nitrapyrin.

Subsurface drainage, a water management practice used to remove excess water from poorly drained soils, can transport substantial amounts of NO3 from agricultural crop production systems to surface waters. A field study was conducted from the fall of 1986 through 1994 on a tile-drained Canisteo clay loam soil (fine-loamy, mixed, superactive, calcareous, mesic Typic Endoaquoll) to determine the influence of time of N application and use of nitrapyrin [NP; 2-chloro-6-(trichloromethyl) pyridine] on NO3 losses from a corn (Zea mays L.)-soybean [Glycine max (L.) Merr.] rotation. Four anhydrous ammonia treatments [fall N, fall N + NP, spring preplant N, and split N (40% preplant and 60% sidedress)] were replicated four times and applied at 150 kg N ha(-1) for corn on individual drainage plots. Sixty-two percent of the annual drainage and 69% of the annual NO3 loss occurred in April, May, and June. Flow-weighted NO3-N concentrations in the drainage water were two to three times greater in the two years following the three-year dry period compared with preceding and succeeding years. Nitrate N concentrations and losses in the drainage from corn were greatest for fall N with little difference among the other three N treatments. Nitrate losses from soybean were affected more by residual soil NO3 following corn than by the N treatments per se. Averaged across the four rotation cycles, flow-normalized NO3-N losses ranked in the order: fall N > split N > spring N = fall N + NP. Under these conditions NO3 losses from a corn-soybean rotation into subsurface drainage can be reduced by 13 to 18% by either applying N in the spring or using NP with late fall-applied ammonia.

Agriculture↗

Solubility of phosphorus and heavy metals in potting media amended with yard waste-biosolids compost.

The potential risk of surface and ground water contamination by phosphorus (P) and heavy metals leached from compost-based containerized media has become an environmental concern. Solubility and fractionation of P and heavy metals were evaluated in media containing 0, 25, 50, 75, or 100% compost derived from biosolids and yard trimmings for potential impacts on the environment. As compost proportion in peat-based media increased from 0 to 100%, concentrations of total P, Cd, Cu, Ni, Pb, Zn, and Mn in the media increased whereas concentrations of total Co and Cr decreased. Except for Cu, all heavy metals in the water-soluble fraction decreased with increasing compost proportion in the media, because of higher Fe, Al, and Ca concentrations and pH values of the composts than the peat. When the media pH is controlled and maintained at normal range of plant growth (5.5-6.5), leaching of the heavy metals is minimal. Incorporation of compost to the peat-based media also decreased the proportion of total P that was water-soluble. However, concentrations of bioavailable inorganic phosphorus (NaHCO3-IP), readily mineralizable organic phosphorus (NaHCO3-OP), potentially bioavailable inorganic phosphorus (NaOH-IP), and potentially bioavailable organic phosphorus (NaOH-OP) were still higher in the media amended with compost because of higher total P concentration in the compost. Further study is needed to verify if less or no topdressing of chemical P fertilizer should be applied to the compost-amended media to minimize P effect on the environment when compost-amended potting media are used for nursery or greenhouse crop production systems.

Fertilizers↗

Nitrate losses in subsurface drainage from a corn-soybean rotation as affected by fall and spring application of nitrogen and nitrapyrin.

Substantial amounts of NO3 from agricultural crop production systems on poorly drained soils can be transported to surface water via subsurface drainage. A field study was conducted from the fall of 1993 through 2000 on a tile-drained Canisteo clay loam soil (fine-loamy, mixed, superactive, calcareous, mesic Typic Endoaquoll) to determine the influence of fall vs. spring application of N and nitrapyrin [NP; 2-chloro-6-(trichloromethyl) pyridine] on NO3 losses from a corn (Zea mays L.)-soybean [Glycine max (L.) Merr.] rotation. Four anhydrous ammonia treatments (fall N, fall N + NP, spring preplant N, and spring N + NP) were replicated four times and applied at 135 kg N ha(-1) for corn on individual drainage plots. Drainage occurred in all seven years. Seventy-one percent of the annual drainage and 75% of the annual NO3 loss occurred in April, May, and June. Fifty-four percent of the NO3 lost in the drainage occurred during the corn phase and 46% during the soybean phase. Annual flow-weighted NO3-N concentrations for the fall, fall + NP, spring, and spring + NP treatments averaged 14.3, 11.5, 10.7, and 11.3 mg L(-1) during the corn phase but annual NO3-N concentrations were still > or =10 mg L(-1) in three of six years for the spring preplant treatment. Averaged across the six rotation cycles, flow-normalized NO3-N losses ranked in the order: fall N > spring N + NP > fall N + NP > spring N. Under these conditions, NO3 losses in subsurface drainage from a corn-soybean rotation can be reduced 14% by spring N and 10% by late fall N + NP compared with fall-applied N. Nitrate losses were not appreciably reduced by adding NP to spring preplant N.

Agriculture↗

A comparison of native tallgrass prairie and plains bluestem forage systems for cow-calf production in the southern great plains.

The objective of this study was to compare an introduced warm-season perennial grass (plains bluestem, Bothriochloa ischaemum) to native tallgrass prairie for cow-calf production. Three systems were used, two based on tallgrass prairie with two different forms of protein supplementation and one based on plains bluestem as the primary forage. The systems were as follows: 1) native tallgrass prairie with pelleted oilseed meal as the winter protein supplement (native-control); 2) native tallgrass prairie with limited access to wheat pasture as the winter protein supplement (native-wheat); and 3) plains bluestem with limited access to wheat pasture as the protein supplement (bluestem-wheat). Oilseed meal protein supplements were fed twice weekly. Cows grazing wheat pasture were allowed 6 h of grazing twice weekly. Ninety-nine cows per year were used over the 3-yr study. Cows were sired by either Charolais, Gelbvieh, Angus, or Hereford bulls out of commercial Angus-Hereford dams. Calves were sired by Simmental bulls. Calving and weaning rate increased over time but did not differ among systems or breed types. System did not influence the size or body condition score of cows or the performance of calves, but changes in the weight and condition scores of cows were greater on either native system than on the bluestem-wheat system. Cows from Charolais and Gelbvieh bulls were taller (P < 0.05), and heavier (P < 0.05), and weaned heavier (P < 0.05) calves than cows from Angus or Hereford bulls. The weight of cows on the bluestem-wheat system tended to decrease over time, whereas cows grazing on the native systems tended to gain weight over time. The native-control system was the most profitable system based on cow production. If excess hay produced from the bluestem-wheat system was sold as a cash crop, then this system was the most profitable. In general, we conclude that limit-grazing wheat pasture is a viable alternative to oilseed meal as protein supplement for wintering dry cows. Although the bluestem system had 2.5 times the carrying capacity of the native prairie systems, increased productivity was offset by increased production costs. All systems were equal on a cow basis for providing nutrients for the cow-calf production system.

Animal Feed↗

Effectiveness of programs to decrease antimicrobial resistance in the intensive care unit.

Resistance of microbes to antibiotics is an increasing problem in intensive care units (ICUs) with a prevalence of 86% in some isolates. Resistance results in increased morbidity, mortality, and increased costs. Risk factors associated with the development of resistance and strategies to combat resistance are discussed. Risk factors include increased antibiotic use, host factors including severity of illness and length of stay, and lack of adherence to infection control practices. Multiple strategies to decrease resistance have been studied. Changing antimicrobial practices via guideline development, antibiotic restriction, use of information systems technology, crop rotation, narrowing spectrum of empiric antibiotics, multidisciplinary approaches, and selective decontamination have had variable results. Prevention of horizontal transmission via handwashing, glove and gown use, alternatives to soap, and improving the workload and facilities for health care workers is discussed. Primary prevention via decreased length of stay, selective digestive decontamination, vaccine development, and decreased use of invasive devices also plays a role.

Anti-Bacterial Agents↗

[Smoking--II. Duration of smoking and factors affecting smoking as a social phenomenon].

According to the poll of 5,555 individuals (91% of the expected number) over 40 years of age, selected from electoral registers in all three districts of the community of Mali Idjos in 1987, the average duration of smoking among active smokers increases with age, divergently with regard to sex. The age of smokers at the time they started smoking increases with age groups, from 22.2 to 33.2 in males and from 28.1 to 49 in females. The onset of smoking in the oldest male group in this rural area occurred in the first years after the war (first land confiscation) while in the group from 70 to 74 years of age it occurred in the years of compulsory crop-purchase system. Agglomeration in the onset of smoking in two male age groups (60-64, 65-69) occurred at the time of the second land confiscation. The onset of smoking among females from all age groups, from 45 years on occurred in the first decade of the sexual revolution (1961-1971). The prevalence of smoking in the studied population was different in previous decades as well. It changes according to social factors: from political and economic changes among males to the changes in women's social status among females.

Adult↗

Influence of packing media on nitrogen removal in a subsurface infiltration system.

Influence of packing media on nitrogen removal in a subsurface infiltration system was studied. System A was filled with loamy soil and system B was filled with mixed soil of 75% red clay with 25% cinder. Both systems were fed with sewage at the same hydraulic loading of 2 cm/d at continuous operation mode. The same excellent removal performances of COD and T-P could be achieved in both infiltration systems with removal rates about 85% and 98%, respectively. In system A, NH4+ -N removal rate was as high as 96.5% and T-N removal rate was relatively much lower as 55.7%. And in system B, NH4+ -N removal rate was as low as 75.4% and T-N removal rate was relatively much higher as 75.5% . The difference was attributed to different soil oxidation-reduction condition that was greatly influenced by soil texture in subsurface infiltration system. Loamy soil led to oxidative condition that was favorable to nitrification and disadvantageous to denitrification. The results were just adverse to the system filled with clay. Intermittent operation was adopted to improve nitrogen removal in system B. NH4+ -N removal rate could be increased to about 95% and T-N removal rate could be increased to about 90% at intermittent operation mode in system B. Analysis of nitrogen removal mechanisms indicated that nitrification-denitrification was the primary nitrogen removal path in subsurface infiltration system and crop uptake was another important nitrogen removal way. It was the key to improve the total N removal performance that a suitable packing soil was available to present favorable oxidation-reduction condition for simultaneous nitrification and denitrification.

Aluminum Silicates↗

Recurrent and niche-specific functional bacteriome of maize hybrid revealed by integrated metabarcoding and culturomics.

The plant microbiome plays a pivotal role in plant survival in natural habitats by facilitating nutrient acquisition, stress adaptation, and disease suppression, while also offering opportunities to enhance crop productivity and climate resilience. However, the distribution of persistent and culturable bacteriome across maize-associated niches and their functional potential remain poorly resolved. This study integrated metagenomic next-generation sequencing (mNGS-based metabarcoding) and culturomics to characterise the maize-associated bacteriome of bulk soil, rhizoplane, phylloplane, and cob of the maize hybrid PHM-1 under contrasting cropping and tillage systems, and to identify recurrent and agriculturally promising bacteriome components. The bacteriome exhibited pronounced niche-specific structuring, whereas overall bacterial community composition did not differ significantly across cropping and tillage treatments (ANOSIM, R&#x2009;=&#x2009;0.038, p&#x2009;=&#x2009;0.306). Proteobacteria predominated in the culturable bacteriome (69-84%; mean, 76.2%) but accounted for only 1% of the total bacteriome, whereas Patescibacteria and Firmicutes were relatively enriched. Niche-specific dominance was evident, with Pantoea accounting for 40.79% of the total and 56.27% of the culturable phylloplane bacteriome under cereal monocropping, while Serratia represented 31.59% and 59.40% of the total and culturable cob bacteriomes, respectively. Across niches, mNGS captured substantially greater bacteriome diversity, particularly uncultured and unidentified taxa in soil-associated compartments, whereas culturomics recovered a narrower but functionally accessible fraction. Culturomics yielded 99 isolates representing 32 species across 12 genera, including six genera shared with the mNGS-derived recurrent bacteriome: Bacillus, Enterobacter, Pantoea, Pseudomonas, Serratia, and Stenotrophomonas. Functional screening identified strong biocontrol and plant-beneficial traits among core-associated isolates. Pseudomonas oryzihabitans ZM-DL-PA10 inhibited Rhizoctonia solani, Macrophomina phaseolina, and Bipolaris maydis by up to 40.6%, 43.9%, and 45.2%, respectively, through secreted and volatile metabolites; exhibited P, K, and Zn solubilisation; and produced IAA and siderophores. It also recorded the lowest B. maydis disease index (ADI) of 1.00. Pantoea ananatis ZM-BH-EA4 showed 52.4% and 68.5% inhibition of R. solani and B. maydis, respectively, through volatile metabolites. Collectively, the integration of mNGS and culturomics revealed a strongly compartmentalised maize bacteriome and identified recurrent, culturable, and functionally promising bacterial taxa, providing a targeted resource for microbiome-based crop protection and climate-resilient maize production.

Zea mays↗

Predicting pesticide residues to reduce crop contamination.

The system of dynamics between pesticides and plants is reviewed, and a conceptual model capable of reflecting the necessary qualitative and structural peculiarities is proposed as a means of predicting residue levels. The degradation processes of various chemical classes of pesticides in plants under different conditions of use are analyzed. Formulas are developed that enable recognition and estimation of residue levels and duration of retention for "new" pesticides and "old" substances under varying treatment conditions. Estimated data are verified to provide positive assessments of the accuracy of the predictions. Mathematical modeling as a means of perception is stressed. With this method, the outlet value can be controlled by changing such inlet parameters as application rate, frequency of treatments, types of plants, and so on. Residue levels are predicted for different combinations of use conditions in various climatic-geographical regions. The method of estimation also enables the development of important standards such as post-treatment waiting intervals. A more flexible technique can be employed, in which specific periods are established for different plants under various treatment conditions. Thus, both unjustified shortened waiting intervals or unnecessarily elongated intervals periods can be avoided. The greatest value of the modeling approach is that information can be obtained on the degree of potential food contamination and major standards can be developed without the need for extensive experimental use of pesticides under actual conditions. This technique fully considers optimal use conditions for agrochemicals in terms of human requirements and protection of plants. Mathematical modeling protects the environment while enabling the speedy selection of safe parameters for pesticide use conditions nd realization of significant savings in manpower and time. Overall, this estimation method appears to be an efficient link in the broad system of preventing environmental pesticide contamination and protecting human health.

Humans↗

The need to look beyond the production and provision of relief seed: experiences from southern Sudan.

Free distribution of seeds in selected areas of southern Sudan has been widespread as a way of increasing food security. Field research in areas targeted for seed relief found that farmer seed systems continue to meet the crop and varietal needs of farmers even following the 1998 famine. Donor investments in seed multiplication of improved sorghum have not been sustained due to a lack of effective demand for the improved seed beyond that created by the relief agencies. The article argues that rather than imposing outside solutions, whether through seed provisioning or seed production enterprises, greater attention needs to be given to building on the strengths of existing farmer systems and designing interventions to alleviate the weaknesses. The case is made to support dynamically the process of farmer experimentation through the informed introduction of new crops and varieties that can potentially reinforce the strength and diversity of local cropping systems.

Agriculture↗

Relationship between leaf area index and yield in double-crop and full-season soybean systems.

Previous research indicates a correlation between leaf area index (LAI) and yield of full-season soybean [Glycine max (L.) Merrill], which is a single crop planted early in the season. Leaf area index values of at least 3.5-4.0 in the reproductive stages are required for maximum potential yield. It is unknown how yields of double-crop soybean, which is planted late into harvested small grain fields, respond to changes in leaf area index. We hypothesized that double-crop soybean would be more sensitive to defoliation than full-season soybean. This study used linear and linear plateau models to describe the yield response of full-season and double-crop soybean to reductions in leaf area index through manual defoliation, and evaluated the yield response of double-crop soybean to reductions in leaf area index through natural insect defoliation. From 15 manual defoliation experiments over 3 yr, significant linear decreases in yield occurred in both full-season and double-crop soybean when leaf area index values were below 3.5-4.0 by developmental stages R4 to R5, whereas yields usually reached a plateau at higher leaf area index levels. Average yield loss was 769 +/- 319 kg ha(-1) for each unit decrease in leaf area index below the plateau; average maximum yield was 3,484 +/- 735 kg ha(-1). From eight field experiments over 2 yr, insect defoliators had no effect on double-crop soybean yield; leaf area index levels were above 4.0 by the developmental stage when leaf area index estimates were taken (R3 to R6). Therefore, double-crop soybean that maintains leaf area index values above the 3.5-4.0 critical level by mid-reproductive developmental stages can tolerate defoliating pests.

Animals↗

Blue light requirements for crop plants used in bioregenerative life support systems.

As part of NASA's Advanced Life Support Program, the Breadboard Project at Kennedy Space Center is investigating the feasibility of using crop plants in bioregenerative life support systems (BLSS) for long-duration space missions. Several types of electric lamps have been tested to provide radiant energy for plants in a BLSS. These lamps vary greatly in terms of spectral quality resulting in differences in growth and morphology of the plants tested. Broad spectrum or "white" light sources (e.g., metal halide and fluorescent lamps) provide an adequate spectrum for normal growth and morphology; however, they are not as electrically efficient as are low-pressure sodium (LPS) or high-pressure sodium (HPS) lamps. Although LPS and HPS, as well as the newly tested red light-emitting diodes (LEDs), have good photosynthetically active radiation (PAR) efficiencies, they are deficient in blue light. Results with several of the crops tested for BLSS (wheat, potato, soybean, lettuce, and radish) have shown a minimum amount of blue light (approximately 30 micromoles m-2 s-1) is necessary for normal growth and development. For example, the lack of sufficient blue light in these lamps has resulted in increased stem elongation and significant reductions in photosynthesis and yield. To avoid problems with blue-deficient lamps and maximize yield, sufficient intensity of HPS or blue light supplementation with red LEDs or LPS lamps is required to meet spectral requirements of crops for BLSS.

Biomass↗

Assessing long-term impacts of increased crop productivity on atmospheric CO2.

A full assessment of the impacts of land clearance and crop production on atmospheric CO2 requires a systems approach. By considering long-term soil carbon changes and fossil fuel energy inputs, we show that increased crop productivity will alleviate CO2 release to the atmosphere primarily by preventing additional land cultivation. Each hectare of cropland undergoing a simulated threefold crop productivity increase here prevents a net release on the order of 150-200 Mg C to the atmosphere over 100 years by avoiding additional land cultivation which would otherwise be required. This effective carbon sink would slowly diminish with time due to fossil fuel energy input requirements. However, future self-containment of the energy needs of high-yield crop production may displace on the order of 1.0 Pg C per year of fossil fuel carbon, in addition to the carbon sink attributable to avoided land cultivation. By avoiding land cultivation, high yield crop systems also preserve natural ecosystems.

Agriculture↗