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Medial prefrontal cortex and pavlovian conditioning: trace versus delay conditioning.

Pavlovian eyeblink (EB) conditioning was studied in both trace and delay paradigms in rabbits (Oryctolagus cuniculus) with either medial prefrontal cortex (mPFC) lesions or sham lesions. mPFC lesions of prelimbic cortex (Brodmann's Area 32) retarded EB conditioning in the trace but not the delay paradigm. However, this effect was significant only when the conditioned stimulus (CS) was 500 rather than 100 ms in duration. Lesions of the anterior cingulate cortex (Area 24) did not affect EB conditioning in a trace paradigm. Accompanying CS-evoked heart rate slowing was attenuated under all conditions by the mPFC lesions, although this result was not always statistically significant.

Animals↗

Effects of contingency violations on the extinction of a conditioned fear inhibitor and a conditioned fear excitor.

Rats were used in a conditioned-suppression paradigm to assess the effects of contingency variations on responding to a conditioned inhibitor (CS-) and a conditioned excitor (CS+). In Experiment 1, various unconditioned stimulus (US) frequencies were equated across the presence and absence of a CS- in the context of either background cues (continuous-trial procedure) or an explicit neutral event (discrete-trial procedure). With both procedures, a CS-alone treatment enhanced inhibition, whereas treatments involving 50% or 100% reinforcement for the CS- eliminated inhibition without conditioning excitation to that CS. The latter outcome also occurred in Experiment 2, with discrete-trial training equating considerably reduced US frequencies for the presence and absence of the CS-. In further evidence that inhibition was eliminated without conditioning excitation to the CS-, Experiment 3 showed that a novel CS did not acquire excitation when 25%, 50%, or 100% reinforcement was equated across the presence and absence of that CS in the context of a discrete-trial event. Using the procedures of Experiment 1, Experiment 4 showed that a CS+ was extinguished by a CS-alone treatment but was substantially maintained by treatments involving 50% or 100% uncorrelated reinforcement. These effects for a CS+ and a CS- implicate CS-US contiguity, rather than contingency, as the factor determining the extinction of a CS.

Animals↗

Influence of visual conditioned stimulus characteristics on the form of Pavlovian appetitive conditioned responding in rats.

The influence of visual stimulus characteristics on the form of Pavlovian appetitive conditioned responding in rats was examined in three experiments. In Experiment 1, localized conditioned stimuli (CSs evoked behaviors mostly directed toward the CS source, and more diffuse CSs) evoked behaviors mostly directed toward the delivery site of the food unconditioned stimulus (US). In Experiment 2, localized CSs nearer the US delivery site evoked more CS-directed but less US site-directed behaviors than localized CSs farther from the US delivery site. In Experiment 3, the extent to which rats contacted CS sources depended on variation in a number of CS features casually described as affecting the feasibility of contact with those CSs. In all three experiments, some differences in behavior evoked by the various CSs were noted prior to conditioning; these differences were enhanced by conditioning and further magnified by discrimination training. These results are related to a hypothesis in which orienting responses play an important role in determining the character of Pavlovian conditioned responding.

Animals↗

Conditioned suppression and conditioned enhancement with the same positive UCS: an effect of CS duration.

Previous experiments have shown that positively reinforced operant responding is suppressed during a conditioned stimulus terminated with an electric shock (conditioned suppression). In the present experiment, the conditioned stimulus was terminated with a positive unconditioned stimulus, and it was found that the duration of the conditioned stimulus was a key factor in determining whether response suppression or response enhancement was observed during the stimulus. The lever-pressing responses of rats were maintained by a variable-interval schedule of food reinforcement. While the rats were pressing the lever, a light was occasionally turned on, its offset coincident with a brief period of access to a sucrose solution. In consecutive blocks of sessions, the light duration was 40 sec, 12 sec, or 120 sec. Results showed that the rate of lever pressing was substantially suppressed during the 12-sec stimulus, slightly suppressed during the 40-sec stimulus, and enhanced during the 120-sec stimulus.

Animals↗

Relative durations of conditioned stimulus and intertrial interval in conditioned suppression.

The effects of the relative durations of the conditional stimulus and the intertrial interval on bar pressing during a conditioned-suppression procedure were examined as a function of two additional variables--type of operant baseline schedule and rate of shock presentation. In Experiment 1, response suppression was compared across components of a multiple fixed-ratio, random-ratio, fixed-interval, random-interval schedule, at relative conditioned-stimulus/intertrial-interval durations of 1/1, 1/4, and 1/9. In Experiment 2, relative conditioned-stimulus/intertrial-interval duration (1/5, 3/3, or 5/1) was manipulated across groups, while shock frequency (2, 6, or 10 shocks/hr) was manipulated within groups. In both experiments, suppression during the signal was virtually complete at all relative durations. Responding was also suppressed during the intertrial interval, but that suppression varied as a function of experimental manipulations. In Experiment 1, intertrial-interval response rates were higher when relative signal duration was 1/9 than when it was 1/1, although both relative signal duration and shock frequency, which covaried, could have contributed to the difference. In Experiment 2, the patterning of response rates between successive shocks was affected by relative duration, absolute rates during the intertrial interval varied as a function of shock frequency, and differences between suppression during the signal and suppression during the intertrial interval were affected by both relative duration and shock frequency. The data support an analysis based upon relationships between shock-correlated and intertrial-interval stimuli and, as assessed by the relative-delay-to-reinforcement metric, are comparable to results that have been reported from experiments using similar manipulations under the autoshaping paradigm.

Animals↗

[Heat and moisture exchangers for conditioning of inspired air of intubated patients in intensive care. The humidification properties of passive air exchangers under clinical conditions].

Heat and moisture exchangers (HME) are used as artificial noses for intubated patients to prevent tracheo-bronchial or pulmonary damage resulting from dry and cold inspired gases. HME are mounted directly on the tracheal tube, where they collect a large fraction of the heat and moisture of the expired air, adding this to the subsequent inspired breath. The effective performance depends on the water-retention capacity of the HME: the amount of water added to the inspired gas cannot exceed the stored water uptake of the previous breath. This study evaluates the efficiency of four different HME under laboratory and clinical conditions using a new moisture-measuring device. METHODS. In a first step, the absolute efficiency of four different HME (DAR Hygrobac, Gibeck Humid-Vent 2P, Pall BB 22-15 T, and Pall BB 100) was evaluated using a lung model simulating physiological heat and humidity conditions of the upper airways. The model was ventilated with tidal volumes of 500, 1,000, and 1,500 ml and different flow rates. The water content of the ventilated air was determined between tracheal tube and HME using a new high-resolution humidity meter and compared with the absolute water loss of the exhaled air at the gas outlet of a Siemens Servo C ventilator measured with a dew-point hygrometer. Secondly, the moisturizing efficiency was evaluated under clinical conditions in an intensive care unit with 25 intubated patients. Maintaining the ventilatory conditions for each patient, the HME were randomly changed. The humidity data were determined as described above and compared with the laboratory findings. RESULTS AND DISCUSSION. The water content at the respirator outlet is inversely equivalent to the humidity of the inspired gases and represents the water loss from the respiratory tract if the patient is ventilated with dry gases. Moisture retention and heating capacity decreased with higher volumes and higher flow rates. These data are simple to obtain without affecting the patient and can easily be interpreted. It was demonstrated that, compared to physiological conditions, the DAR Hygrobac and Gibeck Humid Vent 2P-HME coated with hygroscopic salts-were able to maintain sufficient inspiratory humidity and heat. The Pall-HME, solely a condensation humidifier, did not meet the physiological requirements.

Adult↗

Effect of surface conditioning methods on the microtensile bond strength of resin composite to composite after aging conditions.

OBJECTIVES: This study evaluated the effect of two different surface conditioning methods on the repair bond strength of a bis-GMA-adduct/bis-EMA/TEGDMA based resin composite after three aging conditions. METHODS: Thirty-six composite resin blocks (Esthet X, Dentsply) were prepared (5 mm x 6 mm x 6 mm) and randomly assigned into three groups for aging process: (a) immersion in citric acid (pH 3.0 at 37 degrees C, 1 week) (CA); (b) boiling in water for 8h (BW) and (c) thermocycling (x5000, 5-55 degrees C, dwell time: 30s) (TC). After aging, the blocks were assigned to one of the following surface conditioning methods: (1) silica coating (30 microm SiO(x)) (CoJet, 3M ESPE)+silane (ESPE-Sil) (CJ), (2) phosphoric acid+adhesive resin (Single Bond, 3M ESPE) (PA). Resin composite (Esthet.X) was bonded to the conditioned substrates incrementally and light polymerized. The experimental groups formed were as follows: Gr1:CA+PA; Gr2:CA+CJ; Gr3:BW+PA; Gr4: BW+CJ; Gr5:TC+PA; Gr6: TC+CJ. The specimens were sectioned in two axes (x and y) with a diamond disc under coolant irrigation in order to obtain non-trimmed bar specimens (sticks, 10 mm x 1 mm x 1 mm) with 1 mm(2) of bonding area. The microtensile test was accomplished in a universal testing machine (crosshead speed: 0.5 mm min(-1)). RESULTS: The means and standard deviations of bond strength (MPa+/-S.D.) per group were as follows: Gr1: 25.5+/-10.3; Gr2: 46.3+/-10.1; Gr3: 21.7+/-7.1; Gr4: 52.3+/-15.1; Gr5: 16.1+/-5.1; Gr6, 49.6+/-13.5. The silica coated groups showed significantly higher mean bond values after all three aging conditions (p<0.0001) (two-way ANOVA and Tukey tests, alpha=0.05). The interaction effect revealed significant influence of TC aging on both silica coated and acid etched groups compared to the other aging methods (p<0.032). Citric acid was the least aggressive aging medium. SIGNIFICANCE: Chairside silica coating and silanization provided higher resin-resin bond strength values compared to acid etching with phosphoric acid followed by adhesive resin applications. Thermocycling the composite substrates resulted in the lowest repair bond strength compared to citric acid challenge or boiling in water.

Acrylic Resins↗

Release dynamic process identification for a cement based material in various leaching conditions. Part II. Modelling the release dynamics for different leaching conditions.

This paper deals with process identification and model development for the case of a porous reference material leaching under certain hydrodynamic conditions. Four different dynamic leaching tests have been applied in order to take into account different types of solid/liquid contact conditions corresponding to various real leaching scenarios: monolithic and granular material with sequential eluate renewal, and granular material and continuously renewed eluate with different hydrodynamic conditions (dispersion, residence time). A coupled chemical-mass transfer model has been developed to describe the leaching behaviour under all experimental conditions. Diffusion has been considered as the mass transport mechanism inside the saturated porous material and dispersive convection as that in the leachate. Two specific phenomena have been identified and considered in the model: (i) the early surface dissolution of the material which results in high Ca concentration and (ii) the late weak dissolution of Na and K giving rise to a long-term residual release. The intrinsic material parameters such as the initial concentrations in the pore water and solid phases were determined by applying equilibrium leaching tests and geochemical modelling. Diffusion coefficients for different elements and the late solubility of alkalines have been found to reach the same values in the four tests. The estimated values of the surface dissolution kinetic constant have shown a dependence on leachate hydrodynamics when the thickness of the degraded layer is nearly the same in the four tests (intrinsic parameter of the material). The competition between the four main dynamic processes, i.e. diffusion, convection, late dissolution, and surface dissolution, has been emphasized and compared in the four leaching tests: the hydrodynamic dispersion and the residence time had no effect on the leaching behaviour of alkalines, which is controlled by diffusion, whereas the behaviour of calcium (a major element of the material) was strongly influenced. This has significant effects on eluate pH values and on the concentration of Pb (the monitored pollutant). The model was then applied to simulate a landfill scenario in the case of a stabilized/solidified incinerator residue containing heavy metals and chloride. A high rain infiltration level and the use of small blocs are favourable conditions for enhanced pollutant release.

Construction Materials↗

Conditions for the acceptance of deontic conditionals.

Recent psychological research has investigated how people assess the probability of an indicative conditional. Most people give the conditional probability of q given p as the probability of if p then q. Asking about the probability of an indicative conditional, one is in effect asking about its acceptability. But on what basis are deontic conditionals judged to be acceptable or unacceptable? Using a decision theoretic analysis, we argue that a deontic conditional, of the form if p then must q or if p then may q, will be judged acceptable to the extent that the p & q possibility is preferred to the p & not-q possibility. Two experiments are reported in which this prediction was upheld. There was also evidence that the pragmatic suitability of permission rules is partly determined by evaluations of the not-p & q possibility. Implications of these results for theories of deontic reasoning are discussed.

Adolescent↗

Optimal conditional error functions for the control of conditional power.

Ethical considerations and the competitive environment of clinical trials usually require that any given trial have sufficient power to detect a treatment advance. If at an interim analysis the available data are used to decide whether the trial is promising enough to be continued, investigators and sponsors often wish to have a high conditional power, which is the probability to reject the null hypothesis given the interim data and the alternative of interest. Under this requirement a design with interim sample size recalculation, which keeps the overall and conditional power at a prespecified value and preserves the overall type I error rate, is a reasonable alternative to a classical group sequential design, in which the conditional power is often too small. In this article two-stage designs with control of overall and conditional power are constructed that minimize the expected sample size, either for a simple point alternative or for a random mixture of alternatives given by a prior density for the efficacy parameter. The presented optimality result applies to trials with and without an interim hypothesis test; in addition, one can account for constraints such as a minimal sample size for the second stage. The optimal designs will be illustrated with an example, and will be compared to the frequently considered method of using the conditional type I error level of a group sequential design.

Biometry↗

Sweetness of sweet protein thaumatin is more thermoresistant under acid conditions than under neutral or alkaline conditions.

Thermostability of thaumatin and mechanisms of thermoinactivation were examined at 80 degrees C in the pH range from 2 to 10. The sweetness of thaumatin disappeared on heating at pH above 7 for 15 min, but the sweetness remained even after heating at 80 degrees C for 4 h at pH 2. This indicated that the sweet protein thaumatin is more thermoresistant under acid conditions than under neutral or alkaline conditions. Prolonged heating of thaumatin under acid conditions slowly reduced sweetness, and produced a heterogeneous population of molecules, all of which was soluble and monomeric. The resultant molecules were clearly distinct from those generated by heating at pH above 7. Hydrolysis of peptide bonds and other irreversible chemical reactions slowly took place in the molecule heated under acid conditions, and it would be, in part, a cause of thermoinactivation of thaumatin under acid conditions. The thermostability of thaumatin and the mechanism of thermoinactivation were largely dependent on pH.

Drug Stability↗

A skill-based conditioning games approach to metabolic conditioning for elite rugby football players.

The purpose of this study was to evaluate changes in endurance fitness of elite-level rugby union players (n = 35) undertaking skill-based conditioning games for a 9-week preseason training period. Metabolic conditioning was conducted exclusively in the form of skill-based conditioning games in conjunction with heart rate (HR) telemetry. Two markers of cardiorespiratory fitness were assessed at weekly intervals via the recording of HR responses to an intermittent multistage shuttle test. Significant differences post-training were observed for the percentage of maximal HR (% HRmax) reached during the final test stage and the percentage of HR recovery (% HR recovery) from the end of the final stage to the end of the final 1-minute rest period. Significant improvements were demonstrated for % HR recovery at week 7 (p < 0.05) and week 9 (p < 0.01), and % HRmax in the final test stage was significantly lower at weeks 4, 5, and 7 (p < 0.05) and week 9 (p < 0.01). Further improvements from mid-preseason to the end of the preseason training period were observed for % HR recovery scores in week 8 (p < 0.01) and week 9 (p = 0.012) and for % HRmax reached in the final test stage at week 9 (p < 0.05). These results indicate skill-based conditioning games were successful at improving markers of cardiorespiratory endurance for the duration of a 9-week training period in the elite-level professional rugby union players studied. The HR monitoring was demonstrated to be an effective and practical means of quantifying intensity in the conditioning games format and of tracking changes in cardiorespiratory fitness.

Adaptation, Physiological↗

Vibrio parahaemolyticus growth under low-iron conditions and survival under high-magnesium conditions.

Since 1996, Vibrio parahaemolyticus serotype O3:K6 and closely related strains have been associated with an increased incidence of V. parahaemolyticus gastroenteritis worldwide, suggesting the emergence of strains with enhanced abilities to cause disease. One hypothesis for the recent emergence of V. parahaemolyticus O3:K6 and related strains is an enhanced capacity for environmental survival relative to other strains, which might result in increased human exposure to these organisms. Therefore, the objective of this study was to test the hypothesis that survival or growth characteristics of clinical V. parahaemolyticus isolates differ from those of nonclinical isolates under different environmental conditions. Twenty-six V. parahaemolyticus isolates selected to represent either clinical or food sources were monitored for either survival following exposure to high magnesium (300 mM) or growth under iron-limited conditions. Isolates in each category (clinical or food) differed widely in survival capabilities following 24 h of exposure to 300 mM Mg2+. Although 4 of 15 clinical isolates grew better at approximately 0.96 microM Fe2+ (iron-limited conditions) than at 50 microM Fe2+ (iron-rich conditions), as an entire group clinical isolates in this study were not more effective at growing under iron-limited conditions than were strains not associated with disease. Within the diverse collection of strains examined in these experiments, neither growth characteristics in low-iron environments nor survival capabilities following exposure to high magnesium concentrations were uniformly different between clinical and nonclinical V. parahaemolyticus isolates. Therefore, neither phenotypic characteristic can be used to reliably differentiate potentially pathogenic V. parahaemolyticus strains.

Animals↗

Mortality and work conditions: a retrospective follow-up assessment of the effects of work conditions on the mortality of male employees in the manufacturing industry.

A retrospective follow-up study was conducted to investigate the effects of work conditions on mortality. Mortality rates among male employees aged 40-60 years in the Japanese steel industry were calculated for separate work condition factors, such as job site location (parent company or subsidiary company), job category (white-collar or blue-collar) and work schedule (day work or shift work). Odds ratios were calculated to evaluate the independent effects of work condition factors on mortality using a logistic regression method. Death certificates and demographic statistics, 1991-1995, from the annual personnel reports of the employees which had been compiled and preserved by the company, were utilized. There were 19,642 employees in April, 1991, and 171 deaths and 76,761.7 person-years were observed. No work condition factors were associated with the mortality rates. Selection bias was speculated from the findings. Since there is a thorough medical checkup system at this company, the employees identified with serious health problems would probably already have been excluded as potential candidates for transferring to subsidiaries or for work schedule changes. These findings and the interpretation were consistent with those of other reports which have been conducted in Japanese industrial fields. Mortality was not an appropriate indicator for assessing health risks in the occupational environment, but it reflects the real status of health care management. Through careful interpretation it has been suggested that there is a possibility that past health care work conditions activity can be accessed.

Adult↗

[Characteristic features of adaptation to experimental conditions in primates with various initial keeping conditions].

Ethological study of emotional state and behavior was carried out in male macaques of two species (Macaca mulatta and Macaca fascicularis), which were divided in three groups according to their initial keeping: isolated, family, and gregarious. During instrumental conditioning, the initial keeping was changed for identical conditions (individual caging or sitting in a primatological chair). It was shown that adaptation to stress associated with a sharp enviromental change depended on initial keeping conditions. Monkeys brought up in family with experience of both intra- and interspecies interactions (for example, monkey--human) had the highest adaptation and learning abilities. Individual and gregarious keeping of monkeys impoverished their individual experience, and their adaptation to experimental conditions required additional efforts. The learning of individually caged monkeys was successful, if their keeping conditions were not changed. Initially gregarious monkeys were successfully trained after their habituation to contact with humans.

Adaptation, Psychological↗

Conditional gradient displacements: the effects of conditional discrimination training on human auditory frequency generalization.

The effects of conditional discrimination training on human auditory frequency generalization were examined. In Experiment 1 subjects learned to press a key to one tone, but not to a higher pitched tone when presented in the left earphone, and to respond to the higher pitched tone but not to the lower tone in the right earphone. Generalization tests revealed conditional peak shifts, with subjects responding to frequencies lower than the positive stimulus (S+) for left-earphone presentations and to frequencies higher than S+ for right-earphone presentations. The shifts observed following conditional discrimination were larger than those obtained from subjects who received simple discrimination training. Experiment 2 showed that these larger shifts were not simply due to extended discrimination training. In Experiment 3 subjects were trained with conditional discriminations involving an S+ and a negative stimulus (S-) in one earphone but only a single, positive stimulus in the other earphone. These subjects also showed conditional gradient shifts with displacements in both directions. The occurrence of peak shift without an S- can only be interpreted as involving relational responding.

Adolescent↗

Modulation of acute graft-versus-host-disease after allogeneic bone marrow transplantation by tumor necrosis factor alpha (TNF alpha) release in the course of pretransplant conditioning: role of conditioning regimens and prophylactic application of a monoclonal antibody neutralizing human TNF alpha (MAK 195F).

Contribution of host-related cytokine release in the course of pretransplant conditioning to early tissue damage and induction of acute graft-versus-host disease (GVHD) after allogeneic bone marrow transplantation (BMT) has been shown in experimental models. We performed a clinical phase I/II trial applying a monoclonal antibody neutralizing human tumor necrosis alpha (TNF alpha) during pretransplant conditioning as additional prophylaxis in high-risk patients admitted to allogeneic BMT; TNF alpha serum levels and clinical courses in 21 patients receiving anti-TNF alpha prophylaxis were compared with data from 22 historical controls. Absence of significant release of TNF alpha in the period of busulphan (BUS) treatment, but significant induction of TNF alpha by total body irradiation (TBI) and cyclophosphamide (CY) conditioning were correlated with significantly earlier onset of acute GVHD in patients receiving TBI/CY regimens as compared with BUS/CY-treated patients. Prophylactic application of monoclonal anti-TNF alpha seemed to postpone onset of acute GVHD from day 15 to day 25 (P < .05) after TBI/CY and from day 33 to day 53 after BUS/CY (P < .10) conditioning. Application of monoclonal anti-TNF alpha in low and intermediate doses was safe and not associated with an increased incidence of infectious or hematologic complications. Thus, our data provide indirect and direct evidence for involvement of conditioning-related cytokine release in induction of early acute GVHD in the clinical setting and support further investigation of this novel approach in randomized trials.

Acute Disease↗

Ethanol enhancement of Pavlovian conditioning: comparison with instrumental conditioning.

Yoked pairs of rabbits received conditioning and extinction of eyeblink and heart rate responses using aversive Pavlovian versus instrumental avoidance contingencies. A low dose of ethanol (375 mg/kg) increased the amplitude of conditioned eyeblink responses during training and during subsequent extinction following either ethanol or water; this effect was more pronounced in the Pavlovian than the instrumental contingency groups. Ethanol treatment did not affect the cardiac component of the Orienting Reflex to novel tones or heart rate conditioned responses to tone signals, but did bias heart rate responses to tone-shock stimulus pairs in a parasympathetic direction; this effect occurred in both contingency groups but appeared to involve associative factors. These results support the conclusion that mild ethanol intoxication enhances the acquisition of Pavlovian conditioned reflexes, and impairs the ability to modify these responses when stimulus contingencies later change.

Acoustic Stimulation↗