[Our current experience with sensitivity to acari and to house dust in children].
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The Authors report the milestones which led to the discovery of the mite Dermatophagoides pteronyssinus as the main allergen source in house dust, and discuss its etiological role in allergic respiratory diseases. The present knowledge on the importance of other species of mite potentially allergenic in man is focused. From a biological point of view, the taxonomy, anatomy and physiology of these important Arthropods are described. Furthermore, the techniques for the collection and identification of mite specimens in house dust samples are briefly described. From an ecological point of view, the most important factors which regulate the peculiar distribution of some mite species in the domestic and occupational environments (i.e. relative humidity of air, temperature, sources of food) are described. The Authors, furthermore, report the theoretical criteria and the practical procedures for the control of mite growth in the houses. The main physical, chemical and immunological characteristics of the mite allergens are described. The Authors emphasize the studies on cross-reactions between different mite species, from which the importance of the storage mites as allergens of clinical relevance appears clearly. Furthermore, the techniques for the diagnosis and immunotherapy of the mite-related allergic diseases are briefly described.
The seasonal variations in the number of mites present in the house dust of six homes in Barcelona were studied. The samples were taken at monthly intervals between September 1972 and January 1974. Tables I and II show the total number of mites and the number of Pyroglyphidae present in each of the samples. Table III shows the monthly average of the atmospheric temperature and relative humidity (R.H.). Graphing the average of mites present in the monthly samples and comparing them to the graph of the atmospheric R.H. an almost perfect correlation of the atmospheric R.H. with the number of mites was observed (Graph 1). The statistical analysis of the results shows the existence of a highly significant correlation (alpha less than 0.001) between the average of the atmospheric R.H. of a given month and the decimal logarithm of the number of mites present in the house dust the following month (Tables IV and V; Graphs 4 and 5). The following results can be pointed out: a) The quality of mites in the house dust of Barcelona changes considerably during the year. b) The quantity is greatest during spring and autumn. c) The annual variation differs from year to year. In conclusion, it is shown that the seasonal variations in the quantity of mites present in the house dust of Barcelona are proportional to the variations in atmospheric R.H.
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In Sardinia, a great Mediterranean island, house dust mites allergy is very spread. This investigation was carried out to determine the species, occurrence and abundance of mites in the house dust of sensitized subjects. 55 samples of house dust were examined and the mites fauna identified. Dermatophagoides pteronyssinus was the most abundant (54% of the found mites), and the most frequent mite (94.5%). Two others pyroglyphid species were recovered: Dermatophagoides farinae and Euroglyphus maynei that were common in Sardinian house dusts. The frequency with which E. maynei was found in this region (50%) suggests that he may well be an important cause of allergy in this region underestimated. Several species of storage mites were common in house dust, chiefly Glycyphagid and Acarid species, that have been shown to provoke strong reactions in sensitized subjects. The Mediterranean climate, and its effect on the physical conditions within the house, and the personal life style of the inhabitants are the most important factors associated with high mite numbers in Sardinian house dust.
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In this work we collect the results of the variation of the variable "rechange of the wind with the exterior" in the three possibilities of a bedroom: --close window, semi close and totally open. And we unite the relation that we already know between the prevail of acariens (Dermatophagoides) and the relative humidity of the wind in a Bilbao city's house.
Respiratory allergy and bronchial asthma in particular can be serious afflictions in younger as well as in older children. Therefore interest has been focused on methods for the prevention of atopy. The role and allergenic importance of Dermatophagoides pteronyssinus (Dpt) in house and other environments have been identified. This mite provokes asthma in children at an earlier age than pollens. However the most under-valued area of asthma prevention is environmental control. The steps may seem difficult at first, but the results will be well worth the efforts and the sacrifices. We report on the high effectiveness of the guanine detection test (Acarex) based on the colorimetric quantification of allergen sources in house dust (mattresses, pillows, upholstered furniture, carpets, moquettes, etc), which can be treated with house dust mite extermination products such as benzyl benzoate. The eradication measures will allow the parents to reduce the allergen exposure, to be monitored at 3-6 month intervals. The guanine detection test performed in our Division on house dust samples assembled by the patents of children with house dust mite induced asthma (study group) or pollen asthma (control group) yielded highly significant statistical differences.
Parasitic associations between larval Unionicola foili and the dipteran Chironomus tentans were established in the laboratory, and the effects of these water mite larvae on survival and reproduction of C. tentans were determined. Longevity of parasitized insects was not significantly different from those that were unparasitized. Furthermore, there was no significant difference in the onset of oviposition between parasitized and unparasitized female chironomids. However, infected females oviposited significantly more eggs compared to uninfected controls. This study contradicts others indicating that water mite larvae severely reduce the survivorship and reproductive output of insects. The results are consistent with analyses indicating that degree of parasitism is not correlated with either duration of the parasitic phase or longevity of larvae following their parasitic association with chironomids. Larval U. foili apparently secure sufficient nutritional resources to complete their development, yet their hosts still exhibit fitness components expected for unparasitized individuals. Extreme site specificity exhibited by larval U. foili may limit the degree of parasitism on host insects and in doing so preclude larval mites from achieving infection levels that could negatively impact host survival and reproduction.
BACKGROUND AND OBJECTIVE: Measurement of guanine in dust proved a good assessment of mite allergen exposure. METHODS: Exposure to mite allergens may lead to atopic inflictions. In a semi-natural test system the development of Dermatophagoides pteronyssinus (Trouessart) and Glycyphagus domesticus (De Geer), and the presence of their guanine excretion, was examined in a dust-soiled and mouldy environment. Mites were counted after heat-escape, and guanine was detected by means of capillary zone electrophoresis. For each species, 50 mites randomly taken, were inoculated on soiled test-surfaces of 10 x 10 cm. Rough wooden board, gypsum board, tufted carpet, and a self-made mattress representing wall surfaces and home-textiles, respectively, were used. Eight weeks after inoculation with mites only, the surfaces were all mould ridden, and mite and guanine measurements were taken. The Spearman rank correlation test and the Mann-Whitney U-test were used in statistical analysis. The confidence limit was set at 1%. RESULTS: Among the various test-surfaces, no differences were found regarding total mite numbers and amount of guanine present (P > 0.01). For the dust-eating mite D. pteronyssinus, total mite numbers correlated with the amount of guanine present (P = 0.002) on all inoculated surfaces, indicating feeding on the protein-rich dust. For the mould devouring mite G. domesticus, however, no such correlation was found (P = 0.72). Apparently, they mainly consumed fungal carbohydrates during this experiment. CONCLUSION: The allergological relevance of storage mites has been under discussion for the last 25 years. In humid homes, these mites will feed almost exclusively on fungi and may produce allergenic or irritating substances different from those arising on protein-rich laboratory media used in allergen extract production or present in carpets, bedding and furniture.