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PubMed1959

[Anaplasmosis].

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Anaplasma↗
PubMed2006

[Fever of unknown origin caused by anaplasmosis].

A 74-year-old man in good clinical condition presented with complaints of recurrent fever up to 38 degrees C and diffuse thoracic pain, both present for several weeks. Antibiotic therapy did not result in disappearance of the symptoms. Except for a picture of chronic inflammation and a positive Anaplasma IgM antibody titre, serology yielded no indication ofthe diagnosis. Further investigation, including imaging, showed no convincing cause. 'Anaplasma infection' remained the working diagnosis. One month after hospitalisation, the patient was free of fever without any type of therapy. Control blood tests revealed seroconversion to Anaplasma IgG antibodies, constituting serological evidence of a recent infection. The prevalence ofanaplasmosis is increasing. Mice are the principal reservoir for the intracellular bacteria and the infection is transmitted by ticks. In case of fever of unknown origin, since the transmission takes place in the same manner, one should consider both Borrelia and an infection with Anaplasma.

Aged↗
PubMed1991

Preliminary observations on ticks and tick-borne diseases in the north west province of Cameroon. I. Babesiosis and anaplasmosis.

During a survey on ticks and tick-borne diseases in the North-Western Cameroon at the Bamenda cattle market, the ticks identified were Boophilus annulatus (20%) and B. decoloratus (80%). More than 50% of the ticks were collected during the dry season. Of 524 blood smears 47.3% were positive for Babesia bovis, 31.1% for B. bigemina and 2.2% for Anaplasma marginale. 19.4% were negative.

Anaplasmosis↗
PubMed1985

Development of an indirect fluorescent antibody test, using microfluorometry as a diagnostic test for bovine anaplasmosis.

The indirect fluorescent antibody test for the diagnosis of Anaplasma marginale infection in cattle was modified for use with microfluorometry. The test was standardized by use of a fluorometer that measures intensity of fluorescence. Standardization included A marginale-infected blood smears on microscope slides as antigen, serum from an inoculated calf as a positive control containing specific antibody, and an affinity-purified fluorescein-conjugated anti-bovine immunoglobulin as 2nd antibody. The modified test and microfluorometry allowed for titration of sera from A marginale (Florida isolate)-inoculated cattle with a degree of accuracy exceeding visual determinations. In addition, the fluorometric test was more sensitive than the complement fixation or card agglutination tests in identifying cattle that had previous Anaplasma infections.

Agglutination Tests↗
PubMed1986

Epidemiologic aspects of bovine anaplasmosis in semiarid range conditions of south central Idaho.

The prevalance of Anaplasma marginale-infected cows, as determined by use of the modified rapid card agglutination (MRCA) test, was measured during a 4-year period (1980-1983). The prevalence of A marginale-infected cows, defined as positive reactors on the MRCA test, remained constant (31%-37%). The apparent incidence of A marginale transmission to susceptible cows was approximately 7% from 1980 to 1981, 8% from 1981 to 1982, and no transmission from 1982 to 1983. The occasional MRCA-positive cow became negative on the MRCA test, and 1 cow was determined to be free of A marginale infection by subinoculation of 100 ml of the cow's blood into a susceptible, splenectomized calf. Dermacentor andersoni, a known vector of A marginale, was often found on the cattle and in their environment. However, A marginale was not transmitted to susceptible, splenectomized calves, using collected ticks. Of 56 calves born to MRCA-positive cows, 82% were MRCA-positive within the first 3 months of life. These calves converted to MRCA-negative status and were determined to be free of A marginale infection by subinoculation of their blood into susceptible, splenectomized calves, indicating the passive transfer of colostral antibodies.

Anaplasma↗