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APIC position paper: responsibility for interpretation of the PPD tuberculin skin test. Association for Professionals in Infection Control and Epidemiology, Inc.

The Association for Professionals in Infection Control and Epidemiology, Inc (APIC) is a multidisciplinary, voluntary, international organization of professionals who practice infection control and the application of epidemiology in all health settings. APIC is an international leader in prevention and control of infection transmission.

Humans↗

Multivariate regression analysis of distance matrices for testing associations between gene expression patterns and related variables.

A fundamental step in the analysis of gene expression and other high-dimensional genomic data is the calculation of the similarity or distance between pairs of individual samples in a study. If one has collected N total samples and assayed the expression level of G genes on those samples, then an N x N similarity matrix can be formed that reflects the correlation or similarity of the samples with respect to the expression values over the G genes. This matrix can then be examined for patterns via standard data reduction and cluster analysis techniques. We consider an alternative to conventional data reduction and cluster analyses of similarity matrices that is rooted in traditional linear models. This analysis method allows predictor variables collected on the samples to be related to variation in the pairwise similarity/distance values reflected in the matrix. The proposed multivariate method avoids the need for reducing the dimensions of a similarity matrix, can be used to assess relationships between the genes used to construct the matrix and additional information collected on the samples under study, and can be used to analyze individual genes or groups of genes identified in different ways. The technique can be used with any high-dimensional assay or data type and is ideally suited for testing subsets of genes defined by their participation in a biochemical pathway or other a priori grouping. We showcase the methodology using three published gene expression data sets.

Animals↗

Use of computer interview data to test associations between risk factors and pregnancy outcomes.

Computer interviews have been used in a variety of settings as a means of gathering data and providing health education information. The objective of this study was to determine whether data gathered from a computer interview have predictive validity in determining pregnancy outcomes. Pregnant women (N = 190) completed a computer-assisted interview to provide risk factor information. Medical records were reviewed to obtain prenatal and birth outcome information. Twenty-nine percent experienced prenatal complications and nearly half experienced problems related to labor and delivery. After known risk factors were controlled for, self-reported psychosocial problems, low social support, and substance abuse by the father were associated with pregnancy complications. An unbalanced diet and low social support were associated with lower infant birth weight. Findings suggest that data collected via computer interviews can provide useful risk factor screening information.

Adolescent↗

Vascular lesions in testes associated with male infertility in Cameroon. Possible relationship to parasitic disease.

Testicular biopsies in 40 of 41 infertile males with severe oligospermia in Cameroon presented massive subendothelial "fibrinoid" deposits in the small and medium sized vessels. Fibrinogen, complement and IgM were demonstrated in these deposits by immunofluorescence. Evidence strongly suggestive of parasitic testicular involvement was also observed in 2 cases. It is postulated that the "fibrinoid" deposits are the result of repeated formation and deposition of circulating immune complexes by reaction of antibodies with antigens. These antigens could be of various origins and in the cases described here they could be derived from living or dying parasites in the region. The accumulation and incorporation of the "fibrinoid" deposits may lead to vascular stenosis resulting in chronic ischaemia, tubular atrophy and fibrosis, and finally oligospermia.

Adult↗

Family-based association tests for quantitative traits using pooled DNA.

Interest in whole-genome QTL mapping has spurred efforts to reduce the cost of studies now based primarily on individual genotyping. Pooled DNA tests are a possible solution, and understanding how measurement error affects test power could assist in study design. Here we describe pooled tests explicitly optimised for measurement error, including family-based tests robust to population stratification. Our results suggest that pooled DNA whole-genome screens may be feasible with current instruments.

Chromosome Mapping↗

Toothless tests: associations between the results of routine screening medical examinations and ill-health retirement of firefighters in west central Scotland.

The final routine medical examinations (RME) of 526 full-time firefighters, all male, retiring from Strathclyde Fire Brigade in the decade beginning 1 January 1985 were studied to determine differences between the results of the 328 taking ill-health retirement (IHR) and the 198 completing maximum service (MS). Mean ages at IHR and MS were 48 and 54 years/respectively. This was statistically significant (P = 0.0000), so the results of the third RMEs, performed after the subjects' 46th birthdays, were also analysed. The findings were also reduced into quinquennia. Since the RMEs were weighted towards cardiovascular disorders, the outcomes of the 37 IHRs from arterial disease were sought and compared to the final results of those with MS. In both final and third RMEs, those completing MS were more likely to be non-smokers (60.9 vs 41.4%; 51.6 vs 42.6%; P = 0.01 and 0.015). In the final RME, subjects with MS generally had normal lung function (abnormal results 4.8 vs 10.4%; P = 0.002), abnormal chest X-rays and near vision (22.4 vs 7.6%; 91.1 vs 51.6%; P = 0.000 for both). In the third RME, firefighters with IHR were more likely to have hypertriglyceridaemia (39.6 vs 22.4%; P = 0.004), and reduced distance vision (32.2 vs 21.2%; P = 0.03). Where IHR resulted from arterial disease, sufferers had raised mean cholesterol (6.76 vs 6.20 mmol/l; P = 0.049), raised median triglycerides (2.1 vs 1.55 mmol/l; P = 0.0236), hypertriglyceridaemia (44.4 vs 21.2%; P = 0.008) and less likely to be non-smokers (32.4 vs 60.9%; P = 0.001). Differences between body mass index, systolic and diastolic blood pressure, fasting and HDL cholesterol, electrocardiogram abnormalities, urinary abnormalities and total numbers of risk factors did not reach statistical significance, apart from isolated quinquennial episodes. These results suggest that, except for highlighting known health hazards, current RMEs are ineffective in identifying those at risk of ill-health retirement in this group.

Blood Pressure↗